10/5/19 Day 43. Removed a few fan leaves and lowered the height of the lamp to flowering distance. The plant is just starting to show pistals. No feed today. Think all is good. 11/5/19 Day 44. Fed 1.5L. 3.0ml/L coco A and 3.0ml coco B, 1.0ml silicon, 0.5ml cal mag, 1.5ml boost. Ph 6.0 Ec2.1 run off 0.4L ph 6.4 ec 2.3 So far so good really, if anyone thinks I'm doing anything wrong then give me a shout. 12/5/19 Fed 2L. 3.5ml/L coco A and 3.5ml coco B, 1ml silicon, 0.5ml cal mag, 1.5ml boost. Ph5.9 Ec2.5 run off 1.5L Ph 6.3 Ec2.3 13/5/19 Day 46 fed 1L. 3.0ml/L coco a and b, 1ml silicon, 0.5ml cal mag, 1.5ml boost. Ph6.0 Ec2.3. run off 0.5L Ph6.4 Ec 2.5 Going to start reducing Coco a and b, then adding Pk9/18 soon. 14/5/19 Day 47. No feed today, just did a little more LST and leaf tucking. Think that's it for the LST. May release all the clamps in next few days and drop my net on it. 15/5/19 Day 48. Decided to release the plant from the LST, I have managed to get the hole canopy level with a cig box, so with it hopefully flower any day now it can stretch evenly (touch wood). Also removes a load of fan leaves. Fed 2L. 2.5ml/L coco A&B, 1ml silicon, 1ml cal mag, 1.5ml boost. Ph6.0 Ec2.1 Run off 0.7L Ph6.4 Ec2.9. yikes that's a big jump for my run off on the Ec. So fed again but 1L and changed A&B from 2.5ml/L to 2.0ml/L. Final Ph6.0 Ec1.9 Run off 0.6L Ph 6.3 Ec2.5 Going to try and get the run off down a bit more 2mo. Looks like I might have to feed once a day from this point onwards. 16/5/19 Day 49. Week hasn't gone as expected, I thought pistils would be showing by now. I am going to leave it released from the LST for a while and apply when it starts to get uneven again. I do think I have got the canopy quite even. Fed 2L. 2ml/L coco A&B, 1ml silicon, 0.25ml cal mag, 1.5ml boost. Ph 5.9 Ec 1.6 Run off 1.4 L Ph 6.3 Ec 2.6. This is quite high which I think is caused by my boosting agent. I might ditch sumo boost and try something else.

DAY 65: Whelp, 8 weeks of shaming, abuse, experimentation, more shaming, and neglect-- the Lucky Charms plant is a f***ing female! Absolutely in-shock. I was so happy to learn she's a girl, but at the same moment was filled with so much regret, for how much better I could have treated this plant/how much larger I could have grown it. Just wow. So I took a clone cutting. It was a little small, but hopefully she takes because this plant is more resilient than a cockroach!! The White Widow plant is steady bushin' in the veg area with the little ones. I really love how short and stocky I've managed to get this plant through all the LST. Its starting to feel. like a bonsai tree. It has certainly earned its adulthood after the catastrophe it endured in Week-5. DAY 68: The re-potting of the Lucky Charms stunted its growth as expected, but its got a long way to stretch, so it should be able to utilize most of the new space. Itching to repot the White Widow. She's ready. Im just short on medium and space at the moment :/

Perdi mais uma semente , agora vamos tocar essas duas para ver se elas crescem bem saudáveis e fortes. Estão com um bom tamanho vieram para o quintal, ainda pode ter algum ataque de pássaros mas preciso do espaço na estufa para novas sementes que germinaram. Perdi mais uma só restou uma , mas ela está bem .

Second week of flush strong aromas nice tricombs.

So this week after I gave it under nutrients I apparently gave it too much nitrogen while having a potassium deficiency. Shiney dark leaves, So i fixed that, but some didn't bounce back, and I tried nitrogen. I think they are doing pretty good considering everything I've put them through SO FAR. lol. Nutrients are NPK Raw's total lineup, follow their instructions at first, Fastbuds adjustments as of this week.

Another great week. Thoroughly impressed so far. Flowers are developing nicely and have already started showing some color.

Weather has been rainy and windy so she has been living under a shelter at nights and most days. The climate is getting moisty here so i decides to remove some fanleafs again to keep a good airflow. Buds are getting bigger and they make me wanna sing ' I like big buds and i can not lie..'

28/04/2021 (Day 43) Se realizo un nuevo cambio de solución, las plantas ya están tirando pelos y los leds están al 80%, se observo un gran crecimiento radicular y las paredes del tallo son increíblemente gruesas y duras. 03/05/2021 (Day 48) Las plantas vienen con un crecimiento optimo pero la flora se está tardando demasiado en hacerse presente, se estima que es debido al estrés que tuvo en semana 3 de vege produjo un retraso de algunos días. Esta semana se incorpora Safe Roots al sistema con la finalidad de que deje la solución lo mas limpia posible. También se realizaron bastantes defoliaciones ya que no existía penetracion de luz teniendo en cuenta el tamaño que están ocupando (85% del espacio de cultivo a 30/35 cm de altura) 05/05/2021 (Day 50) Se realizo un nuevo cambio de solución, esta vez utilizando safe roots para limpiar todo el sistema hidroponico. 600w de Citizen a 30cm en 160x80 y la plantas parecieran que si les doy mas luz van a usarla, es increíble la tolerancia a la alta intensidad de ppfd que tienen, gracias a esto logramos subsanar esa semana de raíces marrones. La temperatura bajo drásticamente y nos dejó el indoor en unos 22 grados, donde todo marcha perfecto.

Dopo due settimane senza postare nulla siamo all’ultima settimana di prodotti prima del risciacquo. Tutte e 4 le piante sembrano avere un ottima struttura, una buona ramificazione degli apicali e teste compatte e profumate (a super incollose, senza guanti non si possono toccare. Partiremo a breve con il risciacquo con il ghiaccio per incentivare i pigmenti viola a formarsi ed espandersi su tutta la pianta. Ci aggiorniamo a inizio risciacquo!

Note: At time of writing the Runtz has had 2 weeks cure, will update the review and rate it after 2+ months cure. Hermied a bit, has very few seeds so not too bad. Growth review: - vigorous grower during veg - solid stretch - medium internode distance - small sized buds - lower buds not too airy - very easy/not fussy - medium amount of smell - 80+ days flower (12/12) in hydro DWC under full spectrum led - responded well to various lst techniques Ease of growth rating: 9/10 General note: --> For me personally this strain NEEDS a good cure and is not worth smoking yet... It has a bit of a citrus grassy (not hay) taste, with some 'special' fruity smells to it that remind a bit of grapes/cassis. When grinding/opening the curing pots there is a pretty dank heavy weed smell so I think the quality will come out after a few months of cure... In its current state not worth smoking for the taste or effect after 14 days cure. --> Currently the effect is not very strong, easy to handle and I need a lot to feel a bit. The effect lasts for short compared to the average strains I smoke. Should develop more character with a longer cure. Hash making notes: - water extraction of the trim yielded 16g of hash (45mc-220mc) - rosin has a bit too much of a weird spicy grassy taste which make it a bit unpleasant, waiting till the buds improve with cure to try again - rosin color at 80c is yellow/white/beige creamy waxy, with hard consistency - rosin color at 95c is golden with hard consistency

Day 70/71 gotta install new fans, so we have a good circulation and airflow

Feed 2.56 EC PH 5.65 Runoff RP7 PH6.06 EC 3.2 RP8 PH6.27 EC 1.96 RP6 PH 6.35 EC 1.93 RP5 PH 6.26 EC 3.5 RP3 PH 6.4 EC 1.71 RP4 PH6.5 EC1.75 RP2 PH 6.44 EC 2.44 RP1 PH 6.4 EC 2.12

Ideally you want to drastically up your PK Week 5 of flower. Unfortunately adding PK late last week forced me to ramp up by 2ml/gal every day until 9 ml/gal to avoid shocking the plants. These plants were growing super bushy despite being Sativa dominant. Definitely played it too safe Week 3, gonna go heavy next run.

The buds grow and more pistils are turning brown. O.G. Kush top buds which are just under the light are fox-tailing a little bit but the rest of the buds look nice. I'm watering with BioBloom as the previous week and overall, the leaves are still green so I think it works well. This week, the plants reached the 100th day since the start of the germination :)

Flowering is going well, I removed Big Bud and introduced Overdrive. The buds are already full of resin and as you can see they're already tending to orange, I'm really curious to see what the final product will be like. 💪😋😊

Since week 7, I have used some sewing string and tape with paper towel pieces (so string doesn't imbed) to slightly pulled down on main stems to open up canopy in middle and 3 or 4 fan leaves snipped. The bottom buds in 2 days became like the top buds. Ppfd is like 600 at 13 inches away causing sun Tan on anterior part of stem, or its genetics. 2nd pic is today, DAY 58...... P.S. I journal everything in a book since my 1st grow. Helps me look back on what this strain does not like. Also will upload diary of nothern lights and trainwreck. 1 of each survived out of 4 total. In 4x4 rn. Starting bananablaze, and will have white window started hopefully this weekened. Happy grows and Happy 🌲💨

My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems.

Gave her 45ml of bloom and barley and 5ml of grow and she's loving it.

Smell is getting intense which is a good problem I always like having lol got the carbon filter running 24/7. The GroBucket system really keeps the soil at the perfect moisture level. Made a aeroponics system that’s got me intrigued lol but all and all things are coasting by it’s just on me to keep environment stable and the reservoirs filled. Check out the channel I got one video I need to upload then I’ll be uploading daily on YouTube check me out https://youtube.com/@C_More_Budz