All content on this diary is for inspirational and educational purposes only. The ideas shared are not a substitute for professional advice. This diary/account is not officially affiliated with Alan Watts or his estate. All materials are used under the principles of fair use. I honor the legacy of Alan Watts by sharing his wisdom respectfully and with the intention of inspiring awareness and self-understanding. 2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Ende 6. Blütewoche Die Buds schwellen immer mehr. Dieser Durchgang ist nicht mein schönster ( besonders die Special Kush) aber ich denke das ich trotzdem eine schöne hochwertige Ernte haben werde und verzichte jetzt auch auf großes entgegensteuern mit irgendwelchen Nährstoffen. Ich denke wenn ich jetzt einfach so weiter fahre wie zuvor bring ich beide easy ans Ziel. :)

Pulled the shorter of the 2 Sater day week 11 flower the tall one I’ll flush next week and give her 2 weeks for 13 weeks of flower total! Found one seed on the branch I pollenated with FW10 on the short one I have drying !!

This plant was planted a week after the other six plants in the tent and she stretched very good during the first week of flower, in veg I saw she was gonna get big fast, she grows super fast and ended up the same size if not bigger than her sisters, very recomended strain, amazing powerful smell, very strong cerebral happy high, I enjoy a lot sativas, diesel it's not my favorite flavor but it's definitely awesome for everyone specially you diesel fans! Congratulations to Gea Seeds providing amazing quality strains to the growers!

The water consumption rise significantly since last week. The plant is watered now every 3-4 days with one liter of water. High temperatures and loser humidity are still present, even though not as bad as before. I put the light today to 80% (40 watts) and increased the nutrition dosis nearly to the recommended level by Canna, since the plant is developing faster than expected.

Nothing added this week other than a compost teas with freshly cut alfalfa & fenugreek with a little frass. Growing very fast and very well

24 Jan - 30 Jan Started Flushing on Sunday night (23 Jan) ---- 25 Jan A lot of leaves are starting to die, from all of the plants, maybe due to nutrients maybe due to climate, or maybe is just the strain on the end of its life. Either way, I'm planning to harvest next week.

Promising

Easy trim✂️ The buds are solid like a diamond 💎💍 The smell : citrus pine & rosemary🍋🌲absolutely divine super fresh it’s a one way to the Mediterranean Sea☀️✈️

El lec lo he bajado a 245w por la temperatura.

Ok this was my first grow and I made mistakes. -forgot to buffer coco - nutrient burn -started the manlining to early - probably worried to much about every stage these plants. -ended up with fungus gnats And probably appt more I can’t rmeber in the moment. But i learned so much from all of my mistakes and spent so much time researching and seeing in real time how these plants react and grow. I did do some experimentation just a normal top with one blue dream. I topped twice one another blue dream and did a 3 times topped on all the blue dreams and I see why you should at least top three times the eight main colas and spread out the plant I see some of the buds on the one I only topped once not matured at all because there was minimal light other buds were growing so fat it suffocated it basically. I could have probably defoliated and manliness better but ayy. Beginners wil make Mistakes and I’m happy with what came out of these girls. Not excited for trim hail but I am at the same time 😅😅

Bud forming so fast!!!!!! Comparing to previous plants I growth in witch I used biobizz light soil I changed kind of soil in this one I’m using canna professional and the different is quite noticeable I’m really satisfied. I was worried that the small box I’m growing in would eventually make the plant suffer but it’s totally not, the plant is responding well!! Started with the maximum dose of bud ignitor by advanced nutrient. I’m really exited for this lady can’t wait to se the beautiful complete resinous buds.. keeping growing with LOVE 💚

GOOD MORNING VIETNAM😈💚 Week 6 Day 35: Pics from this noon. Mutants main cola is strecthing like crazy😁 like 30 cm in a week. Not as many white pistils as the other 2 girls. Tb; i topped the 2 other ones. I'm lil worried mutant cola will continue streching till it hits light😁 they continue drinking lots of nute water and no complaints yet😎 One love 💚 Day 37: no nute burn!! I was fully expectin bad nute burn when i fed them on 1600ppm😁 but they seemed to be ok with it ä, although i gave them ph water the next day (36). I might be dumb but seeing the girls are not showing any sings of slowing down or nute burning and all checks out, i gave 900ppm feeding. ( All cal w 0.5 ). Colas have starting to see first snow💚 Day 38: Morning fellow greenthumbs💚 Today i defoliated #3 mutant, did some lst aswell. Gave feeding of 1.9 ec ph 5.7. checked runoff and came as 6.3 and ec 1.9 (might be high from 1600ppm feed😂) no signs of lockout or burns👌👌snapped brach yesterday cos forgot not to train after watering🙁 but got it taped up really quickly and seems like it has maked a recovery😇 Day 39: Had quite busy day as i germinated 2 other strains so no pics. Gave girls ph water of 5.9, 1.8L per gal. No news, mutant is streching but 1 and 2 are full flower and fattening sites😎💚 Day 40: gave #3 and #1 feeding and ph 6.3. skipped plant 2 cos pot was heavy and had still lil bit of runoff. Amazing budsite and frostness development! Even the fan leaves are getting real frosty❄️😎 Day 43: All good , giving 5.9ph 900ppm feedings and check runoff. Beatiful snowy tops😍

Even though the temperatures have gone extremely to high for my liking this plant seems to love it. She’s a very resilient plant I highly recommend tho she strain to everyone. After I clear out my flowering tent I will put this bad baby into flower.

The new additions in the group Pic are LSD auto and Amnesia auto by Barney's Farm. Singen the belong to my Flatmate, I don't know if they will get their own journal.

CHERRY 🍒 COLA BY FASTBUDS Week #7 Overall Week #2 Flower This week has continued a trend of hot weather with no relief for the plants as it's having some effect on the leaves with burning. Stay Growing!! Fast Buds CHERRY 🍒 COLA

Hi Growmies, thanks for stopping by this week for another HighCloudz update ✌️ The plant has been busy growing buds and now they are not only growing in number but in size as well! The top buds are growing bigger by the day, and underneath the canopy the smaller side branches are also forming many buds. The smell is amazing. It's a bit less pepper and more sweet tones at the moment, but boy I'm excited about this terpene profile 👌 Some of the top leafs are looking tarnished because of the nitrogen burn, but luckily there are still many healthy leafs left to prevent any stagnation in growth. Thanks for stopping by and stay tuned for more updates and pics as the week progresses ☘️

Gracias al equipo de Anesia Seeds, Marshydro y XpertNutrients sin ellos esto no seria posible. 💐🍁 Captain Future: Nuestros criadores en Anesia Seeds están siempre en busca de nuevas dimensiones en la genética del cannabis y están comprometidos a ayudar a dar forma al futuro de la cría de cannabis. Después de que nuestra Future # 1 fuera probada varias veces por encima del 37% de THC y se convirtiera en un éxito entre los dispensarios, teníamos claro que el viaje no podía terminar aquí. Después de numerosos intentos, encontramos el socio ideal para mantener o aumentar la potencia y llevar la experiencia de sabor al extremo. Esta variedad Premium puede cultivarse tanto en interior como en exterior. En el interior, las plantas tienen un tiempo de floración de 10 a 11 semanas y deliever rendimientos de 500-600g por metro cuadrado, al aire libre una planta puede producir hasta 800g 🚀 Consigue aqui tus semillas: https://anesiaseeds.com/es/product/captain-future/ 💡FC6500: Eficiencia líder en el mercado: la lámpara de cultivo LED FC-E6500, que ostenta un estatus líder en el mercado, es una solución rentable con un PPE de 2,8 µmol/J y un rendimiento máximo de 2,5 g/vatio. Ofrece un PPF alto de 2026 umol/S y es adecuada para una cobertura de vegetación de 1,50 m x 1,50 m y una cobertura de flores de 1,20 m x 1,20 m. Iluminación versátil y uniforme. https://marshydro.eu/products/mars-hydro-fc-e-6500-730w-commercial-led-grow-light/?gad_source=1&gclid=Cj0KCQjw1qO0BhDwARIsANfnkv9IIrYSbmJqz8PqpJOIyWwJfp5bc3SGJgUV68USLQ4tjmXDYwoBuAsaAue3EALw_wcB 🚥 MarsHydro ADLITE UV/IR/RED: Para lograr un crecimiento óptimo de las plantas y maximizar los rendimientos es un arte simple que depende en gran medida de las condiciones ambientales adecuadas. Reconociendo las limitaciones de la iluminación natural y las soluciones de iluminación tradicionales para satisfacer estas necesidades únicas, lanzamos ADLITE. Estas luces especiales UV, IR y roja están diseñadas para llenar áreas del espectro, proporcionando las altas longitudes de onda que las plantas necesitan para un crecimiento y desarrollo óptimos. 🚀 Consigue aqui tu Adlite: https://marshydro.eu/collections/adlite-supplemental-lights/ 🏠 : Marshydro 1.50 x 1.50 x 1.80, carpa 100% estanca con ventanas laterales para llegar a todos los lugares durante el grow https://marshydro.eu/products/diy-150x150x200cm-grow-tent-kit 🌬️💨 Marshydro 6inch + filtro carbon para evitar olores indeseables. https://marshydro.eu/products/ifresh-smart-6inch-filter-kits/ 🍣🍦🌴 Xpert Nutrients es una empresa especializada en la producción y comercialización de fertilizantes líquidos y tierras, que garantizan excelentes cosechas y un crecimiento activo para sus plantas durante todas las fases de cultivo. Consigue aqui tus Nutrientes: https://xpertnutrients.com/es/shop/ Semana 13: Última semana de lavado de raices antes del corte final, ha quedado un cogollo central enorme, lleno de resina y con un olor apestoso, la dureza de estos es desmesurada. Tanto XpertNutrients como Marshydro han hecho un trabajo increíble, con los Adlite he notado un incremento en la resina y tamaño de las flores.

Buds should be dried in the dark, 50% humidity, at 20 degrees