Day 15 and things are rolling right along.Ive just been giving ph water until the pots are dry.Noticing a lot of growth last few days and they are looking good.Ive decided to go from the 24 hour straight to 20 hours on 4 off light schedule.I have never really noticed much of a difference with auto flower from 18/6,20/4,and 24 hours on the light schedule.I think it is best for the equipment to give them a short rest time.I do like the consistency of the environment with 24 hours during the winter time though :)

In week 10 I have some plants, which have grown taller than the rest (canopy is appr. 80 cm high now). In order to not have to raise my lamp, I rather correct their height by 'supercropping' them. Supercropping is a technique developed by SOMA from SOMA_seeds and works VERY WELL. Instead of topping your plants (=cutting off the main stem) it is better to take the main stem between your fingers (appr. 15-20 cm form the top) and brake the fibers inside and then bend it downwords. This way the stem will stay intact and heal itself and you dont loose the main buds growing on it later. In the video above I show and explain how I supercrop a plant. The plants are now getting more and more into flowering and therefore I switch the dimmer on my SANlight EVO4-120 plants to 100% now (see in video above). The BIO NOVA nutrients are increased as well a little bit, so the plants can build big buds.

May 2nd: She seems to be growing strong. She's trying to grow vertically! Just natural well water. Light intensity is at 75%. No nutes, yet.

Going good so far. I am shocked how little water I have had to feed them honestly. My preperation has paid off very well. 6/13/2024 Day 22 Temp: 82 F RH: 70% VPD: 1.17kPa I went ahead and chopped the middle plant. Mycelium is appearing below and that’s rad! I lowered the plant off the crate I had it on and went ahead and raised the light power to 10/10 because my PAR was good. All plants are showing pistils now. Bloom/Flower has begun. Light distance 18 inches now light power 10/10 50kLux 6/14/2024 Day 23 Temp: 87 RH: 65% VPD: 1.48kPa Plant #1 11 inches Plant #3 8 inches Light to plant 12 and 14 inches KLux at 10/10 65 & 45 Water good, mycelium growing all over. By the time I get back on Sunday the water should be gone, the plants will be huge and I’ll have to hurry to move the light lol. I don’t like how hot it’s staying but I’m afraid to turn it off because how cold it gets at night. 6/18/2024 Day 27 Temp: 75.6 F RH: 58% VPD: 1.25kPa Plant #1 height: 18 inches! Plant #3 height: 12.5 inches 6/19/2024 Day 28 Temp: 85 F RH: 77% VPD: 0.92kPa Plant #1 Height: 19” in Plant #2 Height: 14” in 6/20/2024 Day 29 Temp: 84 F RH: 64% VPD: 1.32kPa Plant #1 Height: 21 inches Plant #2 Height: 16 inches Light is 13” from plant 🌱 #1 Small was sweet today, that seemed new for sure but I think it’s the sugars in the Quillaja extract. I disconnected the humidifier and plugged in the heater into the UIS plug so that I can start controlling the heat at night.

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Hello, nouvelle session, et 1ere expérience cette fois ci en coco. Avec une nouvelle gamme d’engrais de chez Canna, ainsi que le substrat en coco de chez Canna aussi J’ai constaté que les graines de Quick Critical+ ont germées très vite par rapport à la terre, ici, à peine 72h pour les 1ères. Et un joli 100% pour la germination!! Merci dinafem!👍🔥 J’ai pu aussi constaté que le réglage du PH et de l’Ec prennent ici toute leur importance,, puisque pour mes 1er arrosages j’ai oublié de contrôler l’Ec, résultat, légère brûlure sur le bout des 1ères feuilles,, j’ai immédiatement corrigé en contrôlant et en ajustant l’Ec,, les choses devraient rentrer dans l’ordre maintenant. Voilà pour cette 1ere semaine de découverte de la culture en coco,,, A la semaine prochaine... Happy grow...😎

Las hojas mas viejas se pusieron amarillas, creo que puede haber algún problema en las raíces y/o falta de potasio. Aumenté las dosis de PK.

Week 17 (7-10 to 13-10) 7-10 Temps: 18.4 to 23.5 degrees Humidity: 61% to 78% 8-10 Temps: 19.6 to 24.5 degrees Humidity: 60% to 79% Watering: 2000 ml. 9-10 Temps: 19.8 to 23.8 degrees Humidity: 65% to 77% 10-10 Temps: 19.1 to 24.3 degrees Humidity: 55% to 79% 11-10 Temps: 18.7 to 24.2 degrees Humidity: 46% to 68% Watering: 2000 ml. 12-10 Temps: 17.8 to 22.1 degrees Humidity: 54% to 66% 13-10 Temps: 17.6 to 26.1 degrees Humidity: 42% to 69%

#43 clones are maturing too fast I feel. Especially that a mother is ripening normally. I would guess it’s because of poor rooting. It’s the only difference. I keep runoff at around 3 max. Around week 5 the runoff ph started to drop below 6. Even towards 5.5. Both in rockwool and coco.

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Defoliated the inner leaves and noticed her stretch was inevitable. This could be her last week in veg mode as her stretch is extreme and I would like to avoid breaking when shows up the buds.

Transplanted at the beginning of the week, it did a lot of good for them 🌱💪

Well, the Grandaddy black hermed on me in the beginning of week 7 flower. Separated her immediately and did a flush bringing the ppm down from over 2k to around 500. Two days later she was harvested. A little blown she didn't finish up how I wanted her to. 2 more weeks she would of been ready. Trichomes were 5% amber 70% cloudy 25% clear.

Harvest pics. In the glass jar is pineapple gummy. Same breeder as the lilt. I forgot that I popped one of these seeds it was the last from the pack. The hanging dry pic is the rest of the pineapple gummy it was close to the gavita light and was very hairy. The big top next to it is the top of the lilt plant grown under the mars hydro 150w led light. When the feds choped it all down the lilt was given an extra week than the top which was cut off im absolutely pissed about that I didnt use a carbon filter I think the smell got a bit too strong will be back soon live and learn

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Most of this week I was away so the tent was on autopilot. When i got back i was pleased to see all 4 strains where still around the same on development, with this one possibly being the farthest behind but not by much. The wedding cheesecake is the taller of the 2 fastbuds autos. She is starting to throw out some nice colours now. I'm quite confident this one is going to hit my 13 weeks scheduled for it, so not long left. Notes Day 64 nothing away Day 65 autofeed last weeks nutes Day 66 Got back, everything's fine Day 67 manually water Day 68 light strength from 75% to 87% PPFD was 599 Dli was 38.8 now its 692Ppfd 44.8Dli. Day 69 autofeed this weeks nutes Day 70 622Ppfd 40.3Dli Back in a week Take it easy.

It came into my head last night a migjt have been under feeding them by mixing enough feed for 10 litres of water instead of the 20 i meant to account for i will give them a full dose on the same feed as last week 4ml per 10 litre and 1ml of pk for the 1st feed tnis week and see how they look 🤯

As always it's just a pleasure to grow this sativa dominant strain which definitely reminds me of a great indica with her extremely fruity and tropical sweet terpenes, very happy to have her in my garden and to be able to smoke such a high quality strain once again, hope you guys have enjoyed as much as me!

Our journey with the Sticky Orange XXL Automatic strain from Sensi Seeds has come to an end, and it’s time to share the results! 🌱 This was an easy-to-grow strain with no major issues. However, out of three seeds, one didn’t sprout, and another one, although it sprouted, grew abnormally: in just two days, it stretched 10 cm with a thin stem that broke. But our main girl grew from the start with a thick stem and sturdy branches. After 76 days, we harvested 110 grams. 💪 This strain pleasantly surprised us with its strength and unique flavors. The aroma is unforgettable, and the effect is absolutely explosive – it literally pins you to the couch. It feels like you’ve been shot out of a cannon and are flying into space! 🌌 The effect is strong and long-lasting. After just one hit through a water bong, you start considering a second one, but after a minute, you’re thankful you didn’t take it! 😅 Of course, Xpert Nutrients played a big role in our success – thanks to them, we grew such powerful and tasty buds. Thank you for the great results! 🌟 #StickyOrangeXXL #SensiSeeds #XpertNutrients #GrowDiaries

casi tercera semana de floraciòn.