Hey dudes, The final defoliation took place at the beginning of the week. Since then, the lady has just been allowed to grow. I had a bit of a problem with the low humidity. So I helped myself with a 10L bucket, a fogger and a timer. There's nothing else to mention, the plant looks great. What more could I want? Light Power: 100% Day 68 Flower #23 Lollipopping Day 71 Flower #28 Watered with 2,5L freshly brewed compost tea Photosession

I missed fertilizing the needle too much. I hope it doesn't cause much damage.

Hi liebe Community and Welcome Back! 💚🌱 Ersteinmal beschreiben ich eine erstmals aufgetretene Problematikbin der zweiten Blütewoche. Die Pflanze hat Lichtstress! Das liegt an der Überleitung aus dem Wachstumszelt in das Blütezelt. Mann kann es gut an den sich nach oben kreuselnten Blättern erkennen. Die Pflanze will sich vor Austrocknung schützen. Zuvor war die Pflanze einem PPfD von 280 ausgesetzt und ist nun direkt in die 830 PPfD Beleuchtung ohne Abstufung gekommen. Normalerweise erhöhe ich den PPfD über einen zwei Wochenrythmus von Stufe zu Stufe. Ist aber aufgrund der anderen Pflanzen in der Blüte aktuell nicht möglich gewesen. Sie wächst dennoch gut weiter und hat nochmal etwas an Höhe dazu gewonnen. Außerdem habe ich die unteren Triebe etwas gesäubert und nochmal etwas Blattwerk raus genommen. Ihr Wuchsverhalten ist bis jetzt sehr Indica orientiert. Die Umgebungsgegebenheiten sind aktuell zu hoch für das Stadium: ————— 🌞 Temp: 27 🌚 Temp: 18°C bis 19°C 💨 RH: 49% VPD: 1,42 kPa 🏜️ 💡ppfd: 830 mpm ————— Viele Grüße 👋

The ladies are turning to flower now. Going to be a rainy day or 2, so updating the dairy a little early. Girls are all nice and healthy going into flower. Aromas are very strong from these ladies. Can't wait for the full on flower, to see how stinky these can get. AMS has some pretty strong genetics. I have grown many strains from many different providers, but AMS is never a disappointment. Highly recommend them if your looking for seriously potent weed...

I decided to add a bit more of the base bloom nutrients cause this girl is just begging for it, I feel it. Also the healthiest girl I ever grew so yeah im hyped to see how she will do in the next few weeks, about 2 more weeks then we will start with Overdrive, after that I wont be able to tell that its the same plant. Btw shes running through 10l of water in about 3-4 days so yeah thirsty hahah.

Alright, week 8! Blueberry, the healthy one, looks just fine! Im looking a lot forward too see this tall plant get done. The sick one, seems like it has some normal growth, but i am just unsure of the outcome of the quality of the buds.. lsd-25 is still looking great and will be harvested in a week or 2. I will probably start flushing this week. Day 54: found some little orange egg-looking things in the buds of one og the lsd plants. I removed it and cross my fingers it will stay away. The lsd’s also show some minor signs of nute burn, and the other one is a bit light green on some leaves. I found plants that acted similar in other diaries, but for now i gave straight water

It's still too hot outside to do anything to lower the temperature, but despite everything, the girls are doing very well.

Another week has passed. An eventful week in our lives, but the plants came back on track. Some regular water and defoliating made them happy again. I see lots of similarities between the two strains. Where the XL Runtz ae a true XL and sees to adapt a little better to the conditions than the Forbidden Runtz. But the fight isn't over yet. We still have weeks to go and the forbidden Runtz might recover, my two cents is this: Fastbuds are just a little bit less heat resistant and temps went up last weeks with the SP -3000 on high. Also the XL Runz are closer to the intake of cooler air. So it would be fair to trade places what I do coming week. Thanks again, see you next week!

Week 9, final feed and removed the dead and dying leaves. Buds are rock hard, frosty AF, sticky and has a very pungent aroma. The end is nearing and I'm excited to see the final results. Thanks for stopping by growfessors.

Hallo zusammen 🤙. Habe diese Woche 25 g Silicium Flash gegeben. Sie wächst sehr schön und macht keine Umstände

Very easy first grow very happy with results best weed I ever smoked and I can only get better from here so I'm excited to keep seeing more and more progress

23.05. today is the end of the fourth week for the plants, they haven't progressed much since last week, they grew a few cm and gained a little weight, but they are healthy, which is the most important thing... in a few days I will transplant them and top them because some of them have already developed the sixth pair of leaves, so I simply have to top them. turned out... I thought of transplanting them into 10-liter pots and after two to three weeks into 50-liter holes, however, the weather didn't cooperate, and I was already late anyway because of the bad weather, and because of the same, my plants in the fourth week were the same as they should have been in the second, and because of the same, I couldn't even transplant earlier... now that nothing went according to the "plan", of course, I don't know how smart it is to transplant them into 10 L and then into 50 with the fact that it is with me the longest day of the year is June 22, so that actually a week after that it can start flowering (it usually starts at the end of July or at the end of August, but it can be late and start even in September, and it also knows how to plow and start already at the end of June)...now I think that it is better to buy smart pots of 50-60 liters and immediately transplant into them and then just bury them...another problem with that is that the soil that I took for vegetation has food in it for a month days, if I transplant twice, it may happen that it enters flowering, and the food for the vegetation has not yet been used up, and that would be a problem...advices are welcome Stay high!!!

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

for the 2nd week of the euphoria I gave her 2 ml of trimix, which contain mycorrhizae, trichoderma, amino acids and microelements, all organic (is what the brand says)

Hallo zusammen 🤙. Sie wächst sehr schön und macht keine Probleme

She is getting fat and colas are heavy. I started to use ripen, will continue for 5 days and then the remaining week just water.

Bonjour a toutes et tous (cette semaine j ai eu un petit soucis sur une melon gum (bud rot) alors que je ne dépasse jamais 50% d humidité mais je pense le problème viens d autre part (les têtes ont triplé de taille en une semaine et vue qu'elles sont en contact c est pas top pour la circulation d air j ai donc enlevé les 2 têtes et désinfecté comme il le faut , j ai également monté un gros ventilateurs en plus ) Autrement tous ce passe plutôt bien d ici fin de la semaine on passera au flusch voilà voilà les amis j ai enfin trouver la force et le temp de sortir les plantes de la tante (hors mis les 2 plus grande melon gum / et cherry cookie) mais promis je les sortirais très vite.