Strong bud growth this week. Stable temps and humidity. The green gelato is a strong grower but slightly behind the others in terms of bud development.

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

I have now trimmed and hung the plant after 9 weeks of flowering

BLUE CUSH (SEEDSMAN) 70 Days from sprout. She has many thick buds for such a small plant. Trichomes are about where I want them so she will be getting the chop and dry in a matter of days

Day 6 update - adjusted tie down points and watered. Still no pests and no other significant signs of stress. They ladies are happy and healthy. Day 3 update - Two of the plants have started transitioning into flower. Next week BB1 and BB3 will be dubbed in flower. BB2 pheno is strange I just can't quite tell if it's even in flower... it's lanky and strange. Temps have been steady and now that the heat in the home is on I have to watch heat stress again. BB3 has recovered fully now with new growth looking absolutely perfect. No problems with pests or anything else at the moment. ....Previously.... (day 7 of week 3 - day 1 week 4) One feeding of baking soda to raise the PH of the soil. I decided to tie the girls down the last day of week 3. The topped plant (Bb2) looks more like the strain should than the other 2 phenotypes according to pictures. But the BB1 pheno is so bushy it will be a bigger yeilder for sure. The Bb3 pheno has recovered a bit we will see if the baking soda addition to the top watering helps rebound her better. Selective defoliation was done on all 3 phenos with some under growth getting removed and a couple target fan leaves blocking prime budsites.

Nach knapp 72 Stunden Dunkelheit geerntet und gut getrimmt. Komplett mit Harz bedeckt und dazu Gestank sein Urgroßvater . Pflanze 1 brachte 165 g auf die Waage, Pflanze 2 stolze 180 g und Pflanze 3 immerhin noch 145 g.

Tropical temperatures and occasional thunderstorms... Still growing nicely and getting stronger...☀️⛈️💪🌱🍀

Week 13 20 December 2023 Once upon a time in a small town, a group of friends decided to embark on an unusual Christmas adventure. Instead of the traditional festivities, they hatched a plan to grow a special kind of Christmas tree—cannabis. They called themselves the "Green Elves" and secretly transformed an abandoned greenhouse into their winter wonderland. As the weeks passed, they nurtured their unconventional crop with care, each day bringing new challenges and unexpected joys. With Christmas approaching, the friends faced the dilemma of revealing their secret to the town or keeping it hidden. In the spirit of the season, they chose honesty, deciding to share their unique Christmas tale. To their surprise, the townspeople responded with unexpected warmth and understanding. Rather than judgment, they found support and curiosity. The community embraced the unconventional holiday spirit, turning the greenhouse into a festive gathering place. The Green Elves learned a valuable lesson about acceptance, and their cannabis Christmas tree became a symbol of breaking stereotypes and celebrating diversity. That year, the town had a Christmas unlike any other, filled with laughter, unity, and a touch of unexpected green magic. Merry Christmas 🎅 🎄 Heya👍🤙👍🌱 Thanks for reading!!!🤙👍🤙🌱

Back again for another update from down under. Well Its been a week of unusual weather here-actually most of Australia is having bizarre weather ATM with flooding rains mixed in with high 30's heat and damaging winds. This is in part why I now grow more smaller and better trained plants rather than a couple of larger tree's. One bad storm can literally snap a tree in half so unless your ladies are protected from the wind you WILL have issues outside. Train them, keep them low to the ground and stake them just to be safe ok. Mine are just greening up and bushing out nicely this week, not much more to report other than wondering how much longer before they show me those lil white hairs-cmon ladies its nearly feb and your not flowering yet? Any day now im sure. Hash plant is growing a 1-2cms a day easily, ZkittleZ is bulking up now while Caramelo just seems to be growing more and more shoots-Not used to sativias grown like this-do I cut some off to improve quality or leave em alone to improve quantity? I cant even count how many heads there are now. But the big news is my lemon haze variegated X sour Diesel hybrid seems to be staying female so far- no leaf color at all but the first 3rd gen hybrid that doesnt look like either parents but more of a middle ground of the two, its stayed female so far!! Normally only the ones that looked really sour D like stayed female but this one has a lighter leaf color than normal Sour Ds, but also more the leaf shape of the lemon haze side. Please stay female lil miss. See ya all next week.

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Here's my current grow I stared for this fall season. 2x Blueberry 420 auto on the left and 2x Super skunk auto on the right. Using kind soil with my previous grow soil and foxfarm ocean forest on top. Sprinkle with alfalfa seeds and crimson clovers seeds...using 2 closet case LED. Let's see how this goes.

OMG the smells are becoming so intense, from lemon to the sweet caramel mix with chilies and a touch of pine trees, i think i cant put in words what im smelling and were is taking my mind, but i can tel this, what an amazing combination of fragrances that are flying around The tricomes are shouting up as they become fatter and frostier, all cristal clear so far, i think i still have 3 mb 4 more weeks to harvest, lets see 😜 Just calculate my VPD and it’s 0.98 kPa need to increase this up to 1.2 for now s i’m moving my ligth up a bit and see if it works 🙏🤓🙏 Thank you all for following, comment, like and all 🙏 100 likes 😅🙏 🙌🙌🙌🙌🙌🙌🙌🙌 ❤️❤️❤️❤️ Loving this LED Tec 😍 Girls: 1-BlueBerry 2-Alaskan Purple 3-Poyote Gorilla 4-Hindu Kush 5-Whitw Mango 6-Super Glue 7-Badazz Cookies 8-S.A.D. tent -8x8 / 2.4x2.4 but i'm only using 1/2 so 4x4 / 1.2x1.2 Led - Lumatek 465w Compact Pro at 100% All i Grow is medicine for myself, Stay safe, stay tuned and B Happy Peace out D

This week's been exciting, they've grown upward a few inches and started to bush out. I did do a mild defoliation taking off a few lower leaves and tucked some of the top bigger fan leaves. I started adding co2 this week and they seem to really be enjoying the added amount. All in all Happy Growing

🚨 Durbin Thai/Cinderella 99 week 20 update 🚨 🚨 141 days old!! 🚨 🌸 Day 50 of Flower 🌸 So this is a clone I received on Jan 31st. She has been transplanted from a solo cup 2 a 5 gal pot. She had 2 be topped in her second week of rooting do 2 the top frying from to much light in young clone stage. Since the topping this lady has come 2 life!! She has been defoliated 4 times! Most recent defoliation 3/24! She spent her first month feeding just off nutrients in fox farm soil. On 3/2/22 I gave her her first Gaia Green trio feeding!! She has exploded with growth since!! She was defoliated on 3/27 again. She was given her second feeding of @gaiagreenorganics on 4/02. This lady was defoliated on 4/10 and 4/19 and 5/1. She received a all purpose/bloom feed of Gaia green on 5/2. She was also flipped to 12/12 light cycle on 5/2. She received a heavy defoliation on 5/29. 5/30 gave the girl a 15 tablespoon dose of Gaia green worm castings. Installed the new Marshydro Fc-4800 in the tent on 5/31!! She received her full bloom feeding of 5 tablespoons of power bloom on 6/2!!Marshydro Fc4800 was set 2 100% power on 6/13 which is start of week 6 of flower. This is what she looks like today!! 💡 Grow Sponsor💡 @marshydroled__amazon @marshydronnier #marshydro #fc4800 Marshydro equipment-- Marshydro- 4x4 grow tent Marshydro- Fc4800 Marshydro- 6" inline exhaust fan with digital temp and humidity sensor Marshydro- 6" carbon filter Marshydro- 6" Black duct line Other companies in this grow- @foxfarmsoilandfertilizer - 100% ffof @gaiagreenorganics @inkbirdofficial - WiFi temp control @madmanplant- 6" Tent shelves #rvagrown #rvagrower #cannabisgrower #homegrown #hightimes #weed4ptsd #weed4veterans #veterangrown #tricomes #ledgrown #weedstrains #indoorgrow #indoorgrown #tentgrown #supportlocal #growlocal #letsgrowrva #weedismedicine #420 #veterangrower #clonegrown #marshydro #fc4800

It’s another week of drooling here damn these are some juicy looking veggies, already dreaming of smoking them!🤤🤤 I feel it’s starting to get to the end of the line for 2 of my OG’s, will feed m 2 more times before I start the flush. Plan to have m around for at least 2 more weeks hope that’s enough to clean m out. Got another OG with a crazy amount of leaf growth. Don’t know what to think of it but it has a great color and will def need a lot more time to develop buds, same package of seeds, same strain, same veg treatment complete different plant 🤨 1 gorilla (top middle) had the filter dropped on him and lost the main cola, the rest of the buds seem okay but the little twigs on this plant are way to skinny to keep m up right. The gorilla in the top right corner got too close to the sun, I really thought it was a herma or something but I guess it’s just deformed during a week of extreme temperatures. If ya think I should just chop it off leave me a comment 👍🏼 Sooo really dont know if anybody is still reading but I’m gonna split this diary up in 2, I love this site and it would be better to document per strain for my own archive. And the contests only admit 1 strain per diary hehe 😏

I started in with the maximum ppm of about 1500 on a fresh reservoir and a ph of 6.1. I also went full strength with beastie blooms 0-50-30 at 1 tablespoon for 6 gallons of water. The white hairs are turning orange now which is the sign of times to BULK UP! My diary seems to be a little off on this 45-55 day flower as today we mark day 37 of 12-12.

70 days:) I haven't seen her this week:( the photos and video were taken by my friend, it's been very hot all week, 35°, I'll be back to her in a few days ;) then I'll be able to assess how she's doing, good luck to everyone,