Week 8 (17-3 to 23-3) 17-3 Temps: 17.4 to 21.9 degrees Humidity: 60% to 72% 18-3 Temps: 17.5 to 22.2 degrees Humidity: 54% to 65% 19-3 Temps: 17.9 to 22.4 degrees Humidity: 52% to 61% Watering: Both 500 ml. EC: 0.4 Watering: Both 1000 ml. EC: 1.4 Defoliated both plants and attached some shoots to the rack. 20-3 Temps: 18.2 to 22.8 degrees Humidity: 59% to 69% 21-3 Temps: 18.5 to 23.4 degrees Humidity: 57% to 67% Watering: Both 1000 ml. 22-3 Temps: 19.4 to 24.1 degrees Humidity: 55% to 67% Watering: Both 1000 ml. Set the strength of the light to 50% 23-3 Temps: 19.8 to 24.8 degrees Humidity: 58% to 67% Watering: Both 1000 ml. As you can see, instead of removing the leaves, i tucked down all the leaves to open up the canopy. I will see if i like this method as its the first time i try this. I also attached some more shoots to the rack, its looking nicely spread out now :)

to be updated soon

Día 08 las plantas no están estirándose tanto cómo esperaba. La temperatura tampoco ayuda ya que hace mucho frío donde está el indoor. Pero tengo fé. Recién se empieza a notar el estiramiento.

The Ogs still looking good, the blue widows looking a bit better now, but im not happy with them. Lets look what the next week will bring to them.

Looks alright.😎

1.10.2021 Day 8 Black Haze Info: Nutrients: n/a Water: 300ml Temperature: 21*C - 29*C Humidity: 54% - 72% 2.10.2021 Day 9 Black Haze Info: Nutrients: n/a Water: 300ml Temperature: 22*C - 29*C Humidity: 52% - 72% 3.10.2021 Day 10 Black Haze Info: Nutrients: n/a Water: 300ml Temperature: 21*C - 29*C Humidity: 56% -78% 4.10.2021 Day 11 Black Haze Info: Nutrients: 2nd week! Water: Temperature: 21*C - 27*C Humidity: 54% - 76% 5.10.2021 Day 12 Black Haze Info: Nutrients: n/a Water: 300ml Temperature: 22*C - 29*C Humidity: 50% - 68% 6.10.2021 Day 13 Black Haze Info: Nutrients: n/a Water: 300ml Temperature: 21*C - 28*C Humidity: 51% -72% 7.10.2021 Day 14 Black Haze Info: Nutrients: n/a Water: 300ml Temperature: 21*C - 26*C Humidity: 56% - 70%

Cheese and Mexican are a bit behind the other Lady due too the training, strain gentecics traits. This week hopefully blueberry and GDP will depart the greenhouse. 🥦

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (LIME GREEN CHLOROSIS) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.

Aug 9: Lemon Cream Kush is doing fine and is starting to stretch a bit. I’m surprised how much it grew last week. Got the UV led lights from AC Infinity going. The sunlight gets weak here quickly starting in mid-August and the UV helps form trichomes. Used it last year and it seems to help. Nice weather days only as these lights are not intended for outdoor use. Aug 14: rainy last few days. Noticeably stretched last night. Had UV lights going in afternoon after a few days of not using them.

This plant has been growing at a steady constant rate since they sprouted..I have topped them a week ago and they both appear to recovering well. I still feed basic GHE nutrients with the addition of Orca and Terpinator alll at 1/2 per gallon unless the plants ask for more and trust me you’ll know :) anyway they’re gonna be flipped next week was just letting them build up as much as possible.

Not much happening but she's looking good.

In der 7ten Woche wurde Entlaubt um auf die Blüte vorzubereiten. Leider etwas zu wenig, also war am nächsten Tag quasi schon alles wieder voll gewachsen :( Insgesamt lag das Problem in der zu langen Vegetation. Danach lag der Fokus weiter auf Erholung und dem gleichmäßigen ausfüllen des Blätterdachs. Den EC habe ich nach dem beschneiden auf 1,2 gehoben. Voodoo Juice war nach dem Schema dann erstmal raus. Also habe ich auch den Bakterien etwas zufuttern gegeben, damit nicht alle sterben und ich dachte die komplexen Kohlenhydrate und anderen Zusätze aus Bud Candy kommen der Pflanze auch jetzt vorallem nach dem entlauben zugute. Einen Tag nach der Entlaubung habe ich wieder Foliar B-52 ausgebracht mit Break Thru zusammen, um Stress abzubauen und schneller erholen zu lassen.

Dealing with very strong winds this week. Also morning temperatures are below 15°C causing quite a bit of morning dew. Clear tarps are a big help as long as the strong wind doesn’t blow it away ha ha

The OG Kush made it through her final week of flower...56 days in total. Super sticky and smells like a mix of limonene and pinene and a hint of sweetness on the back end. I'm definitely happy I have a couple more of these beans. Super smooth run, chunky resinous buds. Thank you to everyone that visited to check my first Grow Diary out. You made it a really cool experience ✌️☺️. https://www.instagram.com/ao.grows?igsh=MXR1dWZxMWhlNnF6bA== Check out my Instagram for clips I made for this grow.

Last pictures before the 48 hrs darkness. After that it’s chop time. I’ll snap a couple before she goes into drying. ✌️ While these pictures drop I can add some comments.. she has a terrific nose to her.. kinda like fruity candy.. super sticky .. everything one would want in a medical strain. Though, I’ve never tried a true green crack. I’ve heard great things, and as a Sativa lover .. I’m hoping this girl is a gift that keeps on giving.. we’ll see.

Growth, growth, growth…. They are vegging out now! Definitely seeing the size of my tent getting smaller and smaller this week. Getting ready to bloom soon! I do have to note … the funny guys on the side are from a failed attempt earlier grow this year. We def weren’t ready for them… they got cold, the soil wasn’t right. I got all of that right finally but they instantly went into bloom and def weren’t ready. Oh well!

Tag3: die kleine beschleunigt gerade Wachstumstechnisch. Ist jetzt schon 45cm hoch! Läuft! Erste vorblüte ist aufgetaucht. Also geht es jetzt vermutlich in den Stretch. Wenn es 70cm werden bin ich echt zufrieden. Tag5: 50cm erreicht! Wetter war gut. Ich habe ein wenig das Gefühl Zuviel gefüttert zu haben, den Rest der Woche gibt’s nur noch Wasser.

I actually enjoyed this grow cycle and will be popping more Elev8 genetics I have some more strains that is put up in the freezer . Next up will be Star Punch think it Starfighter crossed with Purple Punch . Magic Cherries smoke smoothly and aromas Magic Melon and the taste terps Cherry lightweight. Satisfied ✊🏾💯

The missing star Due to the weight and growth that is different from other numbers But she still has a highlight that is covered with snow Make us all want to have a relationship with this little girl once🍬🍒🍭❄️ This After harvest 14 Day Living soil🥦🥦 Notill💚💚💚