2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Day 62. 13 days after the flip to 12/12 and starting to show pistils from the shoots. Leaves are much healthier than before and they're taking to flowering well. I cant get the temperature any lower at the moment so it will just have to do. Although heatwaves are on the way to the UK! Emergency news, I must relocate and the ladies are coming with me! Big task but must remove the net and then they're coming on a journey with me! I am not sure whether I will put the net back on and try to weave them through or if maybe LST is the way to go from here unfortunately..

The plant started drinking a lot of water, but the flowering is going well. 🌸

Its going great! They are loving life! Huge different from growing autos.. These are some beasts already.. Cant wait for them to develop Peace&Love

I love the strain and my first autoflower did not disappoint. She grew surprisingly bushy, and she really got chunky near the end! Super happy with how she turned out. The extra couple weeks really made a difference and she smells like fruity pebbles. Delicious! Hanging for 5-7 days before stored for curing. Did final trim and storage for the buds, 50 gram dry weight total. They were more on the lighter side as far as density goes. But smell good and the high is great and was fun to grow! Was able to successfully breed her with a Durban Poison male and look forward to seeing the super sativa babies to come. All good things. Here is to the next round, cheers! 😎

APRICOT GORILLA AUTO / GANJA FARMER WEEK #12 OVERALL WEEK #6 FLOWER This week she's stacking nicely as her buds have trichome and are more round in shape. She's got a funky like fruity gas smell to her. Stay Growing!!! Thank you for stopping by and taking a look it's much appreciated!! THANK YOU GANJA FARMER!! APRICOT GORILLA AUTO / GANJA FARMER

Most of them made it only 1 six shooter definitely didnt make it and a cream cookies is a lil stuck. I threw a mexican airlines in the old six shooter cup but just letting it germinate in the soil I havnt done that in a while. They are going to get another feed tmm. The medium is a 50/50 coco/soil mix since I'm using the green buzz and its organic I figured a little soil might be beneficial. Day 7 from germination they should be ready to go into their final pots in the next few days.

The Banana Purple Punch is looking fantastic as it enters its 7th week of flowering! After checking the trichomes, it’s clear this plant needed a bit more time to mature compared to others, but the wait has been worth it.🌱 This week, I’ve started flushing to prepare for harvest. If all goes as planned, it’ll be ready to cut and hang by the week after next. The buds are looking dense and frosty, and I’m excited to see the final result!😊 So far, everything is progressing beautifully, and I’m really happy with how this plant is finishing up.

Plants started growing very fast. 20 hours light a day and 140 ml water for 2 plants so 35 ml each plant. (70ml -35ml one plant- morning, and 70ml -35ml one plant evening). From Monday I will start giving 200ml water 100ml in the morning and then the rest in the evening. Ventilation almost 24 hours, They are getting bushy and the color looks healthy green. I am excited. Purple Punch ( the right one) does not have the smell but the Gelato Cookie D'ohpe started smelling from the 3rd week, it is not strong, it is smooth, enjoyable. I still use trigger spray but I stopped triggering directly on the plant to avoid any fungee or toxicity, I just keep the soil moist. I surrounded them with kitchen foil around the lamp to have a more effective light. It reflects the light and the light goes everywhere. This step has been done because I have only the 60w LED each plant so I try to provide the maximum strength this way, and by keeping close distance also. The lamp is not hot so it ain't gonna burn the plant I had checked it before. I just purchased today a 600w LED because these plants look so good so I wanna provide the best circumstances for them. 4th-week 4th day I a just waiting for my delivery my 600 W grow LED light will come. From today I started watering with 50ml each plant 2 times a day so all together 200ml a day. Plus I always use trigger spray for the soil to be kept wet. They are looking better and better. Nice color and bushy appearance. Wonderful. I realized some holes on the Gelato Cookie D'ohpe's leaf but there is no insect I was checking. I do not really know the reason for that. Tonight I will set up the new 600 W LED so I am changing the current 120W. Big Step forward! It is not that easy that I thought, the lamp is great but the temperature raised up a lot, so needed to buy a fan also and the humidity went down. It is not easy to find the proper solution to be acceptable, good temperature and humidity at the same time. So I decided to program a 6/2 schedule, so 6 hours light on and the fan always with 15 min more, and 2 hours off so I do provide 18 hours light and 6 hours darkness but it is divided. The plants can rest after every six hours and use the energy they received. Last day of the 4th week, LST has been done with the Gelato Cookie D'ohpe, both are growing rapidly, let's see what the 5th week brings! End of he week Gelato Cookie D'ohpe is 25cm, Purple Punch is 20cm😇

Howdy growfessors! Another week has come and gone with the outdoors grow. The ladies took a bit of a beating from the tail end of tropical storm Ida. Going to build a partial greenhouse roof to help keep the rain off them, which will hopefully lower the chances for mould.

Little lady born on 2/21/2020. Really happy to watch her grow. I haven’t been able to grow out this strain before so I’m stoked on it. She’s on day 8 and showing her beautiful self. Let’s see what this journey has for us. Thanks for awesome genetics !!!!

Ready to rock 🚀🚀🚀🚀🌵Breath x 🍒 Cherry Punch 🥊

Defoliated some leaves to let light in, chopped one down early as I run out of bud 🙄 it's good for less than 4 weeks flowering. Have them rice water starch again this week in with there food. A layer of frost is slowly forming 😎 hopefully they will fatten up nice and frost up really good to 🤞

Always R.O water -Day78 watering 2 liter with calmag 1ml/L - Bio organic grow 1ml/L - Bio organic bloom 1ml/L - Floragro micro 1,5ml/L Total 800 ppm Ph 6,3 -Day80 watering 4 liter with calmag 2ml/L - Bio organic grow 1ml/L - Total 300 ppm Ph 6,3 (Drain under 400 ppm👌) -Day82 watering 3 liter with calmag 1ml/L - Bio organic grow 2m/L - FloraMicro 1,5ml/L - Nirvana 4ml/L - BigBud 4ml/L Total 975 ppm Ph 6,3 * I had a problem with the leaves but this is not problem for me :) but When you give too much buds, such problems can happen. Anyway. I washed the soil and I'm at ease👌 time to slowly over:)

The girls have survived another week ❤️ I did a light defoliation and have been monitoring the RH closely. We have had a very damp week here, and the RH kept climbing and had my fan working hard. They are starting to plump up and fill in 😋. They have also started to slow their drinking habits, instead of watering every three days, it has moved to every 4 now. Just a couple of weeks left, I think I will be harvesting these over Christmas 🎄, and will have some great bud ready for the new year! 🎉🎊🍾

F29, se comenzó con el riego de engorde, con una EC 2.6~2.8 y pH 6.4, se han quitado un par de hojas bloqueando sitios de cogollos, los cogollos están muy resinosos, súper frosty, el olor está súper fuerte también.

Tag 25: Ordentlich gewachsen die Pflanze.

12/10 - Moved 6 clones from a 2x2 into the 3x3 - Swapping lights from 150W MarsHydro to Sunraise QB3000 300W lights - Running humidifier during lights on - had some yellowing on the leaves but have since been corrected.