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PR x CR clones

Noticing a bit of changes with leaf size! Looking pretty healthy no signs of root rot, mold, or insects. Adapting pretty well to 24hr light schedule 💚

12. Woche Pheno 2 braucht scheinbar noch, hat das feeding soweit gut vertragen und entwickelt richtig schöne Buds 😍

Only 2 week left on this beauty smells gorgeous and as usual can not wait hasn't let me down all the way through she responds to everything that I done to her great genetics from original sensible seeds couldn't ask for more thanks and come back next week hopefully start seeing some colour peace growmies 💪👍💯

Day 56 Not a lot to update on. Plants are looking good. The EZ Feed settings are still dialed. I've noticed that the front two plants seem to have fatter buds. #1AP (rear left) has mostly orange pistils while the others have either none or very few. #4AP (front left) is purple. I was unaware of the possibility for purple. Still getting a much stronger candy rub from #4AP and #2EZF. I started feeding Fade and PK @1.6 EC today

Transitioning to LED this week, going to ease into it. Topped at 21 days. Growing Lost Pearl and Critical Kush (which I plan to push into flower early.

Poppin Bottlez ranging from 145-165cm. Grim Spodie are very close to 180cm both. Most of them are clearly flowering allready. Poppin Bottlez looking really dense, Grim Spodie are more stretched out. I gave them a little nutrientboost, cause some of them were starting to get a little bit yellow/light green.

6 plants temporary moving out to the new room until new seeds arrive 4 days later: 9 more (all) plants moving out to the new room ____________________________________________ light- and watering schedule: see photos now: 15 plants 3 x Auto Orange Bud > Dutch Passion (early80's) 1 x Haze Berry Automatic > Royal Queen Seeds (2018) 2 x Auto Euforia > Dutch Passion (late 90's) 2 x Auto Bubblegum > TH Seeds (late 80's) 2 x Original Auto BubbleGum > Fast Buds Company (late 80's) 1 x Strawberry Pie Auto > Fast Buds Company 1 x Gorilla Cookies Auto Seed Stockers (2015) 2 x Auto White Widow x Big Bud > Female Seeds setup + strategy: 18 seeds 3 x Auto Orange Bud > Dutch Passion (early80's) 3 x Haze Berry Automatic > Royal Queen Seeds (2018) 3 x Auto Euforia > Dutch Passion (late 90's) 2 x Auto Bubblegum > TH Seeds (late 80's) 2 x Original Auto BubbleGum > Fast Buds Company (late 80's) 2 x Strawberry Pie Auto > Fast Buds Company 1 x Gorilla Cookies Auto Seed Stockers (2015) 2 x Auto White Widow x Big Bud > Female Seeds setup: 18 x gronest 2 liter 60 cm x 120 cm x 180 cm (2 x 4) growtent 4 l humidifier 25 watt axial fan 15 watt clip fan ro-filter bath room with a 50 watts room fan (-> carbon filter not necessary) ...and a lot of odds and ends. grow strategy: max yield by stressing with: 1. tiny shoes (2 l fabric pots standing on 3 plastic rings (4 cm) for max oxygen) 2. tiny growspace (18plants on 0.72 sqm) 3. many strains (8 strains) 4. annoying neighbourhood (mixing old - i.e. bubblegum (late 80's) - with young- i.e. hazeberry (2018) - genetics) 5. no stress by light or food or water 6. unintentionally too much food plus: - no lst - no hst - just leaf tucking - positioning bigger strains (euphoria, orange bud, haze berry) or just bigger phenos on less intense light spots (end of tent + edges) - music-rotation: 24 hrs reggae (for sativas) - 24 hrs classicals (because it's scientifically proofed) - 24 hrs traditionals from the hindukush region (for the indicas) max efficiency (min electricity - max yield): - light: 23 h on - 1 h off - keeping the distance of 18" (45 cm) and dimming to the right par (lux) - value depending on growing stages (see sheet: beginning 185 par ( 10000 lux/100 watt )/end 340 par ( 18000 lux/175 watt )) => ends up in approx. 170 watts in average over max 15 weeks temperatur management: - using approx. max. half of what the lamp can do keeps temperature low: my tsl 2000 is pulling max. 360 watts of the wall - i need only 175 watts, results in less temperature than using a 175 watts lamp - adjusting the temperature by using a humidifier outside of tent: blown in humidity is soaking degrees and is transported out by fan, works much better than doing the same with pure air summer extreme: no humidifier: 36°C / 35 rh - with humidifier: 29°C / 65 rh works for 1-2 weeks of real hot summer days, no mould, no signs of stress in two summers germination: seeding in waterglass spraying "basic"-water: ro-water (22 ppm) + calmag to 180 ppm every 4 - 8 hours until sprouted man versus fungus gnat: 5 days before seeding i'm running the tent with "basic"-watered pots as if there were plants inside - led on, fans on, humidifier on. Fungus gnats coming with the soil might take the chance to come out now for breeding. i'm waiting with a 9 mm rifle. in the past i found 6 gnats in 2 of 8 x 50 l biobizz lightmix bags coming out of the soil. i could eliminate every single gnat successfully ;) rotating harvest: the little ones will be harvested completely when ready. at this point only the top branches of the big ones will be cut...and the new (basic-watered) pots of the new grow will move into the tent. 2-3 weeks later the secod half of the big ones will be cut and the second half of the new grow will move in... nutes: biobizz / soil: biobizz lightmix bloom,grow,topmax,activera,algamic + aminopower instead of bio heaven (too expensive) individual feeding schedule/once a week: veg: 200 - 600 ppm bloom: 600 - 900 ppm water: ro-water with: veg: 100-150 ppm calmag bloom: 150 ppm calmag

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Week 7 👍🤙👍🌱 These Girls are stretching!!!🌱🌳🌳 Jon Dabs Check them out here: https://jondabs.com @jondabs Instagram The Sponsors Check them out here: Dutch Passion https://dutch-passion.com/en/ Hydroponic.co.za https://hydroponic.co.za/hydroponic/harvesting-and-growing-accessories/ Bud Juice https://www.gohydro.co.za The Best Grow https://thebestgrow.co.za Dynomyco https://www.dynomyco.com Nuclear Roots https://www.nuclearroots.co.za Photronic https://shop.photronic.net Special Thanks to Hydroponic.co.za. I buy all my products from them. Best service. Daily updates coming...

Yo les amis. Les fleurs commencent a gonfler et l odeur se développe

After a slow start, the lady has developed great. Gelonede from Zamnesia grows small and bushy, can be trained and tied down very well. After 42 days of rearing, it bloomed for 58 days. The sticky, dark green flowers smell of lemon ice tea.

I just uploaded the near full blossom condition! may be .. i will upload another run

Look

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

This girl is really comming together an frosting up like crazy as her rock solid buds really start to fatten up threw week 6 of flower under the medic grow fold 6