Vamos familia, actualizamos la cuarta semana de vida de estas Runtz de MSNL. Tuve problemas con la temperatura que estuvo alta 28/26 grados y humedad dentro de los rangos correctos, la única queja es la temperatura, 16 horas luz, 8 oscuridad, estiraron un poco, pero van lentas por el momento, que cojan fuerza. El trasplante a sido realizado a la maceta definitiva, 7L con sustrato Top crop. Hasta aquí todo, Buenos humos 💨💨💨

Topping

I want to harvest now!!!! :D

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Day 79 since seed touched soil. All is going to the end. Mars Hydro TSL2000 works nice, plants look good, needed way better positioning or scrog even, now all bottom half of plant is air with SPARKS, still coated like f@ck ;)))) Think will be strong strain. Want to push 10-14 days more, will see how all goes. Maybe i chop in a week ;)))) Day 83. Made video about last watering, tried to show great light, but no space at all, girls too tall for setup. 4 days left and i chop everything. Happy Growing !!!

And the time to defoliate this Mama as arrived, i finally manage to get my hands dirty and manage to get some air and light Going true this beast, i mean just look at the size of this auto, she is huge , and getting her fruits on the way, notice that she was needy so wen changing the water on the autopot reservoir i top feed her with some extra P and K just in case, i will repeat this if i fell needed <3<3<3 Biut for now just look at these beast and ho she is evolving so fast now that she is in full flower mode, heheh i know they say dont defoliate autos, but i also know me and i just love to try out what ever. They say its not possible to do and see for my self if is or is not possible, these time was defoliating autoflowers and i will fast know if was a good thing or not heheh lets see hihihi Haded a mini video from me defoliating her and the rest of the sisters dividing room with her <3 <3 <3 As always thank you all for stoping by, for the love you guys put in the community, in to growing and it all, i am blessed to walk among all of you and am blessed with it all. Genetics : AUTO SOMANGO GLUE @ ADVANCED SEEDS All info and full product details can be find in can find @ https://aptus-holland.com/ https://advancedseeds.com/ https://autopot.co.uk/ https://lumatek-lighting.com/ #aptus #aptusplanttech #aptusgang #aptusfamily #aptustrueplantscience #inbalancewithnature #trueplantscience #growerslove #dogdoctoofficial With true love comes happiness <3 <3 <3 Always believe in your self and always do things expecting nothing in return and with an open heart , be a giver and the universe will give back to you in ways you could not even imagine so <3 <3 <3 More info and updates @ https://growdiaries.com/grower/dogdoctor https://instagram.com/dogdoctorofficial https://youtube.com/channel/UCR7ta4DKLFMg2xxTMr2cpIg <3 <3 <3 Growers love to you all <3 <3 <3

June 7 - We ordered some new lights last week, they are 240w kingbrite samsung lm310h with uv/ir, 3000k, and meanwell drivers. We setup the new room and moved the girls into there. After a bit of LST and a watering (with nutrients) at roughly 7ph, they were ready to go under the 2 new lights and the same SF-1000 we have been using in this grow. The new room is a 12 ft enclosed trailer. I moved everything from the small tent into this. I put clear poly on the walls, floor and ceiling. I put poly on the shelf I am going to be keeping in there as well. I bought 50ft of 6mm mylar and lined the floors, roof and sides with it. I plan to get some reflective tape to seal everything and to cover the wood. I did not get much of a chance to watch temp's today as they it was later in the evening after all was said and done. The inline fan blowing in air from outside. June 8 - 9 AM i checked the plants, they were at 25.2 and 50% RH. Late through the day I had checked and we had gotten up to around 32 degrees. So I moved the inline fan to the closer vent, hooked up to that, and had the air blowing out of the trailer with the inline fan sucking it from above the lights. I then added oscillating fan and had it blowing air on the left side of the room so it would somewhat circulate once I closed the doors. I then checked at about 9pm and we were at 22 degrees. Definitely noticed a decent amount of growth already. I have the lights on a 22/2 cycle as I was worried about the heat at night time. June 9 - Some great growth from the girls, still having problems controlling the temps in the afternoon. I decided to prop the door open a bit to have a constant breeze throughout the day while I am at work. Decided to do some more LST and also a bit of defoliation. I took about 25% of the leaf's that were blocking the new growth as it was getting a bit bunched up. I was then told the leaf's are almost solar panels for them. So from now on I will be trying to just do some tucking unless needed. The leaf's I removed were most of the damaged leaf's, I am not to sure if that makes much of a difference. I gave them a watering with only water as there is a potential I am getting a bit of a nutrient buildup along with the PH problem. Or potentially the reason for the ph problem is nutrient buildup. After watering with A PH of 7 I got some run off and tested it. The smaller plant gave me a PH of about 5.5 where the bigger one is around 5.0. June 10 - Plants are looking happy and showing tons of growth. Seems to be trying to stretch outwards. Not a ton of sign of PH issues showing so potentially getting it under control. Still a bit of damage to previous leaf's but it is what it is! The last few days I have been leaving the door open a bit in order to keep the temps down. I decided to test something and turn the lights off (automatically) at 11AM and back on at 5PM so light schedule has now changed to 18/6 and it seems I may have figured out the issue. We haven't had lots of sun the last couple days so it hasn't been to hard and I have yet to know if it truly fixed the heat issue for now. (I will be looking into a ac unit as well since it typically gets to around 30-35 around here. June 11 - Pulled some of the branches back down and added a few more LST spots. Seems we have a good amount of growth from the smaller plant out of the 2 topped spots. Unfortunately it looks like I fucked up on the bigger plant and only one of the nodes seems to have new growth. I will continue to monitor that but I think I cut the node to low and also to soon. Other then that, the girls are doing great. They seem to be absolutely loving these new lights. I gave them some nutrient water today as well. roughly 3L each. They seem to be A hell of A lot more thirsty under these new lights. June 12 - The girls are doing great , they are still just doing growing away. Lots of progress everyday. I am going to be getting a go-pro so I can set-up a time-lapse for the rest of this grow. I received my new inline fan, I got A ac infinity CLOUDLINE T4 with the temperature humidity controller. I am going to be having one fan pushing fresh air in and one fan pulling out the hot air. I will be doing that tomorrow since I have to work today. June 13 - I ordered another 50ft of mylar and that showed up today. I have decided to remove the shelf and add another 3+ feet to the grow space. So today I installed more poly, mylar, the ac infinity fan. I have it set-up to pump in air if it gets to warm. I am thinking of switching it to the output that way if it gets to hot or to humid I can have that air pulled out of the trailer. Right now my other inline fan is the outtake and I just have it set on full. Overall I think everything is set-up a bit better and more accessible. I will be putting my 2x2 tent in there at the left of the doors so I can have a veg room when these 2 are in flower. I plan to have 4 in veg and 4 in flower for the next grow. Still waiting on JOTI seeds, ordered 3 weeks ago and still have not been shipped. I will be getting those going the moment they arrive. The girls seem to be happy still, did a bit more LST to try to keep everything even, mainly I'm just pulling down on the spots I already have tie wire on.

Smelling like sweet hard candy! Gonna give her a few more days to leech out a little bit and then cut it down. I can’t wait to try it out! It’s probably the nicest looking plant I’ve ever grown.

Fattys are growing and seem to be thirstier.Used foliar spray to help the leaves and also started tucking. When the plant was topped pistils were seen for first time.

Wieder 2Wochen rum, pro Woche ca 10cm dazu gekommen. Mängel zu erkennen, CalMag gekauft ActiVera wird folgen.

Nice strain, smells like a dank citrus 🍊

Day 42, start of flowering week 3: they are growing nicely, had to do a lot of defoliation on each plant, except the one in the 3L pot. Day 43, I started to discover that the roots are coming out aggressively from the bottom of the pot. I did not move the ones in the back, but I guess same thing. The one in the 3L pot doesn't have this problem. Day 45: getting really excited for these guys! I'm trying to keep the temp and humidity low, and I hope I can achieve some of the purple color :P any advice on that? day46: defoliaton

Was good

😍😍😍🌺

👤 -Las estoy dando aplicaciones de PK booster compost tea,15 gramos por litro((una cucharada)). Aireando la mezcla 36 horas con bomba de aire y piedra difusora antes de regar. Van dos riegos con esta mezcla,quedarán unos cuatro más.

Hello growmies 👩‍🌾👨‍🌾🌲🌲, 👋 The flowering is in progress Week was a bit a disaster... I've lost almost all my leaves in few days, more and more Yellow really fast, like a senesence. The friend @trimva , told me was maybe locked by salt accumulated, so I've tried to did a flush. I don't really understand what's happened with this one, after the flush, look under control 🤞🤞 We'll see next week how will progress... 💧 Give water each 2/3 days. 1,5l Water + Flash Cleaner PH @6 💡Mars Hydro - SP 3000 100% 47 cm. 🔥 Thanks community for follow, likes, comments, always a pleasure 👩‍🌾👨‍🌾💚🌲. Mars Hydro - SP 3000 💡💡 https://www.mars-hydro.com/sp-3000-samsung-lm301b-greenhouse-led-grow-light Zamnesia - Frosted Guava Auto🌲🌲 Discount code -20% with GROWITGD code 🔥🔥 https://www.zamnesia.com/7679-zamnesia-seeds-frosted-guava-automatic.html

The plant took off this week. I am relatively new so was working through getting my PH measuring/balancing down. Now that I have established a better method the plant responded well. Put in a scrog to help even the canopy for the future. The other plant I have running is a bubblegum autoflower that is a few weeks older (right). Personally I really loved the look of the gold leaf at this point, it had a very nice spiral look to it almost like an xmas wreath.