mrjones - Slurricane #7 S1 🌱Slurricane #7 S1 @inhousegenetics_official 👨‍🌾🏽GD Grower: MrJones 🔹🔹🔹🔹🔹🔹GOALS🔹🔹🔹🔹🔹🔹 🌞Environment - 75/80℉ and 60% Humidity Good Old Mother Nature 💧 Feeding - The Green Sunshine Company - Earth Dust All-Natural Plant Nutrients ⚗️Soil - 35% ProMix MP / 25% Ocean Forest / 20% Tupur Royal Gold / 10% lobster Compost / 10% Additional Perlite 🍃Training / So this girl was 60 Inches and spread her out under a 5x5 Trellis 🕷️ IPM - Will be using Green Cleaner" 1 OZ per Gallon, and CannControl from Mammoth alternating between product each month 🔹🔹🔹🔹🔹🔹🔹🔹🔹🔹🔹🔹🔹🔹🔹 📜 Rambling - Week 16 / This lady was already 60 inches tall, built a 5x5 Trellis, and spread her out, with the whole summer to go she is going to Beast Mode On Me! Going to be top dressing with the Green Sunshine Company - Earth Dust All-Natural Plant Nutrients, Two-part Dry Amendment, Base, and Bloom, will be making compost teas each week, see recipes below, will be doing a vegetarian focus tea, and a flowering focused tea. 🔹🔹🔹🔹🔹🔹🔹🔹🔹🔹🔹🔹🔹🔹🔹 ▶️ Sunday - 05.23.21 / Watered today with Dechloranted H20 filtered through a Boogie Brew 40k Inline Filter ▶️ Monday - 05.24.21 / Started a compost tea today - see the recipe below. ▶️ Tuesday - 05.25.21 / Watered plants with the compost tea today, they drank it up! ▶️ Wednesday - 05.26.21/ Plants looking perky! Rain some today. ▶️ Thursday - 05.27.21 / Outside is going well, and a bit yesterday and today, no need to water :) The plant is just looking amazing, will be adding a clone from this one over a few days, this one will be all-natural! The Earth Dust is smoking! ▶️ Friday - 05.28.21 / ▶️ Saturday - 05.29.21 / 🔹🔹🔹🔹🔹🔹🔹🔹🔹🔹🔹🔹🔹🔹🔹 Earth Dust - DESCRIPTION 🔹Earth Dust is our all-natural plant nutrient designed for indoor and outdoor flowering plant growers. You can grow easily by “just adding water” to your soil for the entire growth cycle. It creates a “living soil” so your plants thrive from seedling all the way through flower and harvest. Earth Dust represents an organic dry amendment method of gardening, it’s made of a natural powder of ingredients. 🔹Because you only need to water your soil with Earth Dust, you can concentrate on caring for your plants and getting a healthy crop “on autopilot”. Now you can grow without spending hours of your life mixing chemical nutrients, and you can do it naturally. What’s more, is you will enjoy the cleanest, most aromatic, and flavorful harvest you’ve ever experienced when a plant grows in a rich, organic web of living soil. Product Highlights 🔹All-Natural Ingredients – Mostly plant-based – Safe around children and pets. 🔹Packaged in kraft paper bags that are recyclable and compostable. 🔹Easy to Use – Just mix the powder into the soil 3 separate times – then water only. 🔹Packed full of rich microbe food & contains billions of beneficial fungi and bacteria! Creates a resilient, living soil that defends against pests and diseases. 🔹Rich in trace minerals and nutrients for full plant expression. Sourced from rock dust, sea-farmed kelp, and land-grown crops. 🔹Soil Conditioners help balance pH and make nutrients more available in the soil. Achieved with ingredients like bat guano, worm castings, limestone, molasses, and humic acid. 🔹The correct balance of N-P-K nutrients for each stage of growth: 🔹Earth Dust Base (3-1-2) contains key nutrients for sustained vegetative growth. High nitrogen (N), Low Phosphorous (P), and Medium Potassium (K). 🔹Earth Dust Boost (2-3-6) contains accelerated-release nutrients for powerful flowering growth. Low nitrogen (N), Medium Phosphorous (P), and High Potassium (K). 🔹Re-use soil harvest after harvest by simply adding more Earth Dust Base to begin again. Create your own living soil that gets better every time it’s used. 🔹Go organic, save time, and get predictable, healthy harvests. 🔹🔹🔹🔹🔹🔹🔹🔹🔹🔹🔹🔹🔹🔹🔹 Compost Tea for Vegetative Stage Water 4 Gallons 3 Cups Composr (Your Favorite Worm) 4 TBL Molasses 4 TBL Seaweed Extract 4 TBL Fish Fertilizer Use air Stone o the tea for 24/3 hours 70/75F, use at full strength for establishing plants and a half strength for younger plants

Thrips infection might prevent these lovely flowers from showing their full potential... Will start flushing passively the coming week. Will switch to 11/13 the coming week. There are two distinct phenotypes in terms of flowering time visible as the pics show.

Last week was very challenging, two girls got really tall below the white light, while the other two below the blurple spectrum ended up being smaller and bushier, both looking fantastic. Last week I also probably went too far defoliating the shaded smaller girl which stunned it a bit for a day or two but its flowering vigorously again. Although the unexpected stretch which led to uneven light coverage overally the platns are looking solid, smelly and sugary :)

Normal!!

👀👀👀

The girls are growing great a lot better than I expected so I guess that’s a good thing. Switching to flower in couple weeks this shall be exciting stay tuned

Finally flipped and HOLY MOLY is it awesome to start seeing the flowers coming in for the first time on your first grow. Super delayed flip to flower and I am manually switching the lights/opening tent every day due to prioritized spending. BIG HUMIDITY PROBLEM. As I have still not hooked up an outtake fan for the tent I don't see my girls surviving past the next couple weeks so hopefully I can get that figured out. I'll open it up to 95% RH by morning and it sits around 60% even with an open tent. Other than that, I'm happy with the bud sites gained from my first ever grow. Just feeling my way through Topping, Fim, SCROG, and LST was fun a rewarding for sure. Just have to make sure they reach the finish line. TUES: Cleaned tent floor and walls with disinfectant wipes, seemed to get rid of the smell so far. Still worried about the humidity in the closed tent during the dark period. Need a duct fan hardcore before the buds start coming in. The first sight of trichs already sent me into a Powdery Mildew panic. 😱 THURS: Humidity break. Down in the 45% range and have been able to keep it in the 80s with a huge bowl of dried pasta in the tent lmao no coffee whitener or rice on hand 😒 Defoliation of light blockers and bunch ups to improve the airflow in the canopy. I'm hoping the UV lights in my LED set up are enough to keep mildew at bay and can get the dark period RH% down before buds fatten and there is risk for rot.

Did a reservoir change. Found a bunch of seeds at the top buds, did not find any nanners or balls, most likely somewhere hidden in the bud or non at all and still seeds. The other plant in the same tent does not have a single seed on it. Values are average of the day. DATE - EC(us/cm) 20250528 2009 20250529 2030 20250530 2117 20250531 2125 20250601 2217 20250602 1396 20250603 1533 DATE - PH 20250528 6.13 20250529 6.13 20250530 6.15 20250531 6.15 20250601 6.17 20250602 6.16 20250603 5.92 DATE - ORP (mV) 20250528 174 20250529 163 20250530 142 20250531 131 20250601 108 20250602 151 20250603 116 DATE - °C - RH% (Tent Temp/RH) 20250528 24.8 54 20250529 24.7 51 20250530 25.7 55 20250531 24.3 60 20250601 26.4 55 20250602 24.3 48 20250603 25.2 46 DATE - °C (Reservoir) 20250528 21.1 20250529 20.8 20250530 22.0 20250531 21.5 20250601 22.5 20250602 19.9 20250603 21.9 DATE - CF 20250528 20.09 20250529 20.30 20250530 21.17 20250531 21.25 20250601 22.17 20250602 13.96 20250603 15.32

14. Woche, P1 fällt morgen, die ist soweit gut denke ich (ca. 20% aufwärts Bernstein Trichome). P2 braucht leider noch ca. 1 woche länger. 93. Tag ist für P1 das Ende, sie wurde heute vorgetrimmt und zum trocknen aufgehängt. Ergebnisse gibt es wenn die P2 auch geerntet wird, denke nächste Woche um die Zeit. Aber eins kann ich euch versichern, is mit Abstand die schwerste die ich ist jetzt hatte😊🍀🍀. Gebt euch auch mal wie viele Trichome die gute produziert hat, Duft is noch sehr pflanzlich, Blüten sind ziemlich fest gewesen hoffe das es nach dem trocknen noch so ist. Alles in allem super Run. Hoffentlich is die gute bei den Top 10 das wäre ein träumchen, wobei ich es wirklich jedem gönne zu gewinnen und bei soviel starker Konkurrenz geht ich wahrscheinlich unter. ABER was ein geiler Contest trotzdem, hier ein dickes fettes Dankeschön an Zamnesia und Plagron für die Möglichkeit hier überhaupt mitmachen zu dürfen 🙏💙💜 98. Tag , werd die gute am Samstag/Sonntag je nachdem wie ich Zeit finde, umlegen und zum trocknen aufhängen.

Final and longest week lol I dried these lady’s for 7 days in the tent I grew them in. The tent was around 73F-75F temperature. I used a humidifier to keep the humidity in between 55 and 60. I hung them on the trellis netting. Once they dried I trimmed and put them into jars.

Login issues led to missing a couple weeks, but things have been super smooth. Buds are looking sweeeet and hairy. And they're really fattening up. She's one thick hairy lady. Following the nutrient schedule without deviation, swapping and cleaning the reservoir every week (H2O2 clean), and keeping the humidifier filled. I swapped the pump. First one randomly stopped working for no apparent reason. New one I spec'd with a much lower pump rate and it's a great improvement. No splashing, just a trickle recirculation so I keep it on 24/7. The pH monitoring is so so so much easier now with the good Apera pen. What a difference it makes over the generic ones.

Quite a sad run to be honest, but it's all due to bad decisions on my side. I decided to top her and even though she seemed to take it well, it might have triggered something. She also was a stretcher with such a large inter-nodal space that I didn't find necessary to defoliate anything and I didn't remove any budsites. The result was a ton of small popcorn buds. And to not help, I waited way too long before giving her nutes, thinking I could do all the run without using any but even when I started giving her some, the schedule was close to being improvised. I would normally have given her more time to flower but a space problem in the tent caused me to chop her down prematurely, still with a majority of cloudy trichomes and some amber ones. I would definitely redo some stuff but she still outputted some very decent flowers, effect and taste wise because let's be real, they look terrible, almost like seaweed. I'd love to retry this strain.

So this week the little gelatocake is on week 8 and is really starting to eat herself now, full of trichomes and smells like tutti fruity chewie sweets. Last week of nutes for her then mollases only. Week 7 for the othere gelatocake and shes starting to fatten up now as well as fade also. Babys have got big since re potting I will.bend them over later. Sherbert mimosa are still taking there time I think they are starting to get bigger 🤔🤣 I Will keep you updated lol. Happy growing guys 👌💪💚

Love every plant each one is growing differently stacking different even the visual difference of one strain growin two different ways combined smells of diesel, pez candy and lemons

Flowering Day 66 Harvested today. I'm really happy with the run. Towards the end of the flowering phase, the plants didn't look 100% happy. I'll add more perlite and pumice next time, as I suspect the soil was a bit too moist for too long due to the Autopots. The whole plant will be dried in one piece. I'll dry one in DryFerm bags for testing purposes. Update to follow. Stay green!

Did some more LST on the bty. Going to let it veg until march before i flip to 12/12, as long as its tall enough. I accidentally topped one of them during lst so it will be an expirement to see how it goes. Light at about 65% inensity until i can keep the tent cool at 100% intensity. Plants looking good, getting a little nutrient deficient so the soil is pretty much done. Will start their feeding regiment with florafex full tilt nutes next week

7/21 330am MONDAY everyone is looking good. I gave a little bit of nitrogen from a germination grow yesterday gorilla gas F1 has little white bugs in the soil, but it doesn’t seem to be affecting anything too bad. I don’t want to spray anything. It will affect the outcome and make everything taste gross. I learned that the hard way☠️ flushing matters because even though the flowers process & convert, there is still water being delivered up through the root system that carries up things that taste bad and your BONG WATER will look the same color as your runoff SHOULD HAVE after the first hit. DONT DO THAT, DONT BE LIKE ME!👍👍 7/22 3:22 AM I got five Mike Tyson clones. They are not autos, but I think they are gelato. They are great. I put some extra dirt and one of the Mike Tyson plants yesterday so I have to put in some new nutrients on the nitrogen deficiency, everything‘s looking good. 👍👍. 7/22 1030pm......WELL WE KNOW WHAT DOESNT WORK...... I SCREWED UP WITH RAPID START. IT WILL CAUSE VERY SPECIFIC BURNS TO THE LEAF TIPS AND SIDES WHEN USED. I MAY BE USING TOO MUCH, 0.5ML SHOULD USE .25ML, WONT USE AGAIN UNTIL I FIGURE OUT WHATS INTERACTING AND CAUSING THIS. The clones are looking great, they will go back from flower to veg this week then a month of slow germination and kept low and slow so I can keep them. 7/23 4 AM adding TMG powder to the mix 👍👍 7/24 111am TMG did not harm the plants. The clones are progressing nicely. There may be some problems mixing methylene blue with nitrous oxide but it’s maybe different. There’s a combination of amino acids. I’m still trying to work out. Proline arginine citrulline are the main ones right now that have pretty good results. Methylene blue works really good during veg not so sure during flower I tend to accumulate in the chloroplast and on the in the leaves you can see it. It doesn’t affect the flavor or the taste. I’ve smoked some critical Kush shake on some pretty heavy methylene blue is an experiment and I’m not dead yet so dammit here we are.👍👍👍🎉🎉. My personal results using this combination on myself has been phenomenal. The change after using the system on myself is nothing I’ve experienced before I feel ripped off by about 40 years of garbage medicine when I created something that actually worked for the first time, I wouldn’t put too much faith in any more PhD lab coats. They are all failures and I found a system that works.👍. 845am PROP WALL UPDATE, I GET DISTRACTE....OH LOOK AT THAT... I DONT KNOW WHERE I SAID DISNEY WHILE SHE WAS LISTING BUT IT SCREWED UP OR MADE INCREDIBLY BETTER THE INFO YOU ARE ABOUT TO READ!!!!!!! IM PRETTY SURE I SAID DIXIE VIBE...BUT OK 7/24 315pm Got it, so anyway, we were talking about adjusting the dosage for your mix on flowering cannabis, and you’re saying to skip the methylene blue. Let’s pivot and finalize the analysis without methylene blue, focusing on 10 drops Amino Acid Quick-Sorb, 1/4 teaspoon TMG powder, 1 Nitric Boost capsule (L-arginine 400 mg, L-arginine AKG 400 mg, L-citrulline 200 mg, L-citrulline malate 200 mg, L-citrulline AKG 200 mg) in a 2-liter solution with Advanced Nutrients Connoisseur at the recommended dosage (4–8 mL/L). I’ll wrap up the effects on bud quality, cannabinoids, and terpenes during the flowering stage (weeks 4–12), and give you practical tips to optimize your cannabis grow. ### Effects During Flowering Stage (Without Methylene Blue) 1. Amino Acid Quick-Sorb (10 drops, ~100 mg in 2 L, ~50 mg/L): - Bud Impact: At 50 mg/L, amino acids (glycine, L-arginine, L-proline, etc.) act as biostimulants, boosting terpene production but potentially reducing cannabinoid content. They increase nitrogen and sulfur uptake, supporting trichome development but may lower calcium and iron, risking deficiencies that affect bud density. (https://pmc.ncbi.nlm.nih.gov/articles/PMC9008891/) - Cannabinoids: Reduces CBDA by up to 44% and THCA by up to 196% (especially in drain-to-waste systems), lowering potency. Recirculation systems mitigate this loss slightly. (https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.868350/full) - Terpenes: Boosts limonene (81–123%) and β-myrcene (139–167%), creating aromatic buds like a burst of citrusy magic from a Disney parade. (https://pmc.ncbi.nlm.nih.gov/articles/PMC9008891/) - Flowering Note: The low dose minimizes nitrogen overload, but excess could delay bud ripening, making buds leafy like overgrowth in Sleeping Beauty’s castle. Monitor for yellowing leaves (calcium/iron deficiency). 2. Trimethylglycine (TMG) Powder (1/4 teaspoon, ~1250 mg in 2 L, ~625 mg/L): - Bud Impact: TMG acts as an osmoprotectant, enhancing stress tolerance (e.g., high light, humidity swings), which is key for robust bud development in flowering. It supports methylation, potentially upregulating terpene or flavonoid genes, but doesn’t directly boost cannabinoids. (https://en.wikipedia.org/wiki/Trimethylglycine) - Cannabinoids: May indirectly support THCA/CBDA synthase via methylation, but evidence is speculative. No significant direct impact. - Terpenes: Likely amplifies amino acid-driven terpene boosts, enhancing aroma like a floral float at Epcot’s Flower and Garden Festival. - Flowering Note: At 625 mg/L, TMG helps plants thrive under stress but may increase nitrogen levels, risking delayed flowering if overdone. Watch EC to avoid burn. 3. Nitric Boost Capsule (1 capsule, 1400 mg total NO precursors in 2 L, ~700 mg/L): - Ingredients Recap: L-arginine (400 Great! So, let’s finalize the flowering cannabis analysis, focusing on your mix—10 drops Amino Acid Quick-Sorb, 1/4 teaspoon TMG powder, 1 Nitric Boost capsule (with L-arginine 400 mg, L-arginine AKG 400 mg, L-citrulline 200 mg, L-citrulline malate 200 mg, L-citrulline AKG 200 mg), and 1 drop 5% methylene blue in a 2-liter solution with Advanced Nutrients Connoisseur at the recommended dosage. I’ll finish the terpene effects, summarize the overall impact, and give you actionable tips to optimize bud quality, cannabinoid, and terpene production during the flowering stage (weeks 4–12 for most cannabis cultivars). Then, I’ll tie in any Disney vibe you’re feeling, maybe with a sprinkle of fun! — ### **Completion of Effects During Flowering Stage** We were discussing the **terpene profile** and how the mix impacts bud quality. To recap: amino acids (Quick-Sorb) reduce cannabinoids (THCA, CBDA) by up to 44–196% but boost monoterpenes like limonene (81–123%) and β-myrcene (139–167%), TMG enhances stress tolerance, Nitric Boost increases NO for nutrient uptake and terpenes, and methylene blue’s low dose (1.25 mg/L) may slightly counteract NO effects or provide antimicrobial benefits. Let’s finish the terpene section and synthesize the rest. - **Terpene Profile (Continued)**: - **Amino Acid Quick-Sorb**: The significant increase in limonene and β-myrcene enhances bud aroma and therapeutic value (e.g., anti-inflammatory, sedative effects). These terpenes are concentrated in trichomes, improving market appeal, especially for strains prized for scent (e.g., OG Kush, Blue Dream). Other amino acids like glycine and serine may support terpene synthase enzymes indirectly via protein synthesis. - **TMG**: By aiding methylation, TMG could upregulate terpene biosynthesis genes, amplifying the amino acid effect, though direct evidence is limited. - **Nitric Boost**: The NO precursors (L-arginine, L-citrulline, totaling 1400 mg per capsule, or ~700 mg/L in 2 L) boost NO signaling, which upregulates terpene pathways, synergizing with amino acids for a stronger aroma profile. - **Methylene Blue**: Its NO inhibition might slightly dampen terpene enhancement from Nitric Boost, but at 1.25 mg/L, the effect is negligible. Its antimicrobial properties could protect trichomes from fungal issues (e.g., bud rot), preserving terpene quality. - **Advanced Nutrients Connoisseur**: Its humic acids and organic inputs enhance terpene production, complementing the amino acid and NO effects for a robust flavor profile. (https://pmc.ncbi.nlm.nih.gov/articles/PMC9008891/, https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.868350/full, https://www.advancednutrients.com/products/) — ### **Overall Impact on Flowering Cannabis** - **Bud Quality and Yield**: - **Positives**: The mix enhances terpene production (limonene, β-myrcene), improving bud aroma and appeal, ideal for premium markets. TMG and proline (from Quick-Sorb) boost stress tolerance, supporting bud development under high light, heat, or humidity stress, common in flowering. Connoisseur’s optimized NPK (e.g., ~194 mg/L N, 59 mg/L P) ensures dense, resinous buds. Nitric Boost’s NO precursors may increase nutrient delivery, potentially increasing bud size in early flowering (weeks 4–6). - **Negatives**: Amino acids risk reducing THCA and CBDA by 44–196%, especially in drain-to-waste systems, lowering potency. Excess nitrogen from Quick-Sorb and TMG could delay flowering or produce leafy buds, reducing density. Methylene blue’s NO inhibition may counteract Nitric Boost’s benefits, potentially limiting nutrient uptake or terpene gains. High EC (estimated 1.8–2.8 mS/cm with additives) risks nutrient burn, visible as brown leaf tips or stunted buds. 7/25 9am Water today no MB, all aminos tho. 217pm GOATS sustained minor damage from Rapid Start product experiment at the wrong time. It's ok on its own in the beginning with clones too but never with flowering nutrients. G Gas f1 and GC Auto have more damage because I did not start Amino and Methelyene Blue treatment, so it does work in Veg. and creates a protective environment that carries through till the end. No more MB or Aminos, I WILL keep using small amounts of TMG Powder. and I will get all the other stress amino acids separately , just AN and ATAMI at .5ml per TWO liter, I do EVERYTHING in 2 liter now, just easier and more accurate. NEVER FUCKING SLEEP...FUCK 745PM.... AMINO'S HAVE BETRAYED ME!!!!!! FARGING BASTAGES!!!! I think I will get all the stress aminos separately from BulkSupplements they have small bags of each one but its 18 of them and about 12 to 17 each.....BulkSupplements. 137am. Something smoking Purple Haze...puts me to sleep....nooooo. ZUP by Fastbuds smell like. garlic cheese muffin its just greatl. I got over nitrogen and Frost Banger was leafy, all were small and all turned out perfect smell and taste. 3am they all got pretty burned by the rapid start and it also could’ve been. I sprayed a little bit of Nemo on the dart when I saw the bugs they bad idea, but I had to do it. 7/27 1233am. IVE SEEN RE-VEGES YOU PEOPLE WOULDN'T BELIVE ... UNTIL NOW LOSERS.. the Tyson plants are re-vegging perfectly!!!! in a couple months they will be strong and small. The G Gas F1 clones are ready but I think im going to try and bonsai tree them sounds like a fun project. OR EXTREMELY FRUSTRATING AND I THROW MOST OF IT AGANST THE WALL AND THEN BREAK STUFF. NOT SURE ITS WORTH IT. 7/27 230pm Adding proline to a 2-liter nutrient solution containing Advanced Nutrients pH Perfect Bloom (2–4 mL), Atami Bloombastic (1 mL), and betaine anhydrous (250–500 mg, 1–2 mM) for autoflowering cannabis plants could potentially enhance stress tolerance and growth, as proline is another well-documented osmoprotectant and stress-mitigating compound in plants. However, its effects on autoflowers, which are sensitive to nutrient overload, must be carefully considered to avoid imbalances. Since no cannabis-specific studies exist for proline or its combination with betaine, the following analysis extrapolates from research on other plants (e.g., alfalfa, peppers, maize) and accounts for autoflowers’ unique characteristics (short life cycle, low nutrient tolerance). Background on Proline • What is Proline?: Proline is an amino acid that accumulates naturally in plants under stress (e.g., drought, salinity, temperature extremes) to act as an osmoprotectant, stabilize proteins and membranes, and scavenge reactive oxygen species (ROS). It complements betaine’s role as an osmolyte and stress mitigator. • Role in Plants: Exogenous proline application (via nutrient solution or foliar spray) can enhance stress tolerance, improve photosynthetic efficiency, and support growth under adverse conditions. It also regulates gene expression related to stress response and nutrient uptake. • Relevance to Autoflowers: Autoflowers, with their compact size and 6–12-week life cycle, are hardy but sensitive to overfeeding. Proline could enhance resilience in stressful environments (e.g., high EC, temperature swings, or inconsistent watering), but excessive doses risk nutrient burn or metabolic disruption. Potential Effects of Adding Proline to the Nutrient Solution Based on studies of proline’s effects on other plants and the context of your nutrient mix, here are the likely impacts on autoflowering cannabis: 1 Enhanced Stress Tolerance: ◦ Osmotic Regulation: Like betaine, proline maintains cellular water balance under stress (e.g., drought, salinity). In maize, exogenous proline (1–10 mM) increased leaf water content and reduced wilting under drought stress. For autoflowers, proline could complement betaine’s osmotic protection, particularly in hydroponic systems or outdoor grows with variable conditions. ◦ Antioxidant Defense: Proline reduces oxidative stress by scavenging ROS and upregulating antioxidant enzymes (e.g., superoxide dismutase [SOD], peroxidase [POD]). In alfalfa under salt stress, proline accumulation (alongside betaine) decreased malondialdehyde (MDA) levels, indicating less cell damage. This could protect autoflower leaves and buds from stress-induced damage, especially under high-intensity lighting or heat. 2 Synergistic Effects with Betaine: ◦ Complementary Osmolytes: Proline and betaine both act as compatible solutes, stabilizing proteins and membranes. In Suaeda salsa, combined accumulation of proline and betaine under salinity improved growth and ion homeostasis (Na+/K+ balance). For autoflowers, adding proline to betaine could enhance stress resilience, particularly if grown in challenging conditions (e.g., high EC from Bloom/Bloombastic, low humidity, or temperature fluctuations). ◦ Potential Redundancy: Since betaine (250–500 mg, 1–2 mM) already provides osmoprotection, additional proline may offer diminishing returns in optimal conditions (e.g., controlled indoor grows with 18–20 hours light, 70–80°F, 40–60% humidity). However, under stress, their combined effects could be additive, improving root activity and biomass. 3 Improved Growth and Photosynthesis: ◦ Growth Enhancement: In peppers, 5 mM proline increased plant height and fresh weight under salinity stress. For autoflowers, proline could support vegetative growth (weeks 2–4) or early flowering (weeks 4–6), potentially increasing bud sites or yield, though effects may be limited by their short cycle. ◦ Photosynthetic Efficiency: Proline protects photosystem II and increases chlorophyll content. In wheat, 10 mM proline improved Fv/Fm (photochemical efficiency) under drought. For autoflowers under high-intensity lights (e.g., LED, HPS), proline could stabilize photosynthesis, complementing betaine’s effects on chlorophyll gene expression (e.g., Capsb genes in peppers). 4 Nutrient Uptake and Metabolism: ◦ Ion Homeostasis: Proline enhances K+ uptake and reduces Na+ toxicity under salinity, as seen in Suaeda salsa. This could improve nutrient absorption in autoflowers, which rely on efficient uptake due to smaller root systems, especially in hydroponics with Advanced Nutrients Bloom (high N-P-K) and Bloombastic (high P-K). ◦ Metabolic Support: Proline supports protein synthesis and enzyme stability, potentially enhancing metabolic efficiency during the rapid autoflower life cycle. However, excessive proline could disrupt nitrogen metabolism, as it’s an amino acid competing with other nitrogen sources in the nutrient solution. 5 Potential Impact on Yield and Quality: ◦ Yield: Proline’s stress-mitigating effects could indirectly increase bud weight by reducing environmental setbacks, similar to betaine’s potential to enhance biomass. Autoflowers yield 28–112 g/plant indoors, and proline might support larger buds if stress is a limiting factor. ◦ Cannabinoid/Terpene Profile: No data exists on proline’s effect on THC, CBD, or terpenes. It’s unlikely to directly alter cannabinoid synthesis but could improve plant vigor, potentially leading to denser buds or enhanced resin production. Overapplication risks altering flavor if not flushed properly. Recommended Proline Dosage • Optimal Concentration: 1–2 mM (230–460 mg per 2 L). ◦ Calculation: Proline molecular weight: 115.13 g/mol. 1 mM = 0.001 mol/L × 115.13 g/mol = 0.11513 g/L = 115.13 mg/L. For 2 L at 1 mM: 115.13 mg/L × 2 L = 230.26 mg (rounded to 230 mg). For 2 L at 2 mM: 115.13 mg/L × 2 × 2 L = 460.52 mg (rounded to 460 mg). ◦ Rationale: Studies (e.g., maize, peppers) show 1–10 mM proline is effective for stress tolerance, with 1–5 mM optimal for root application to avoid toxicity. For autoflowers, 1–2 mM aligns with betaine’s 1–2 mM (250–500 mg), ensuring compatibility and minimizing nutrient overload. Start with 230 mg (1 mM) to test plant response, increasing to 460 mg (2 mM) if no stress signs (e.g., leaf burn, stunted growth) appear after 1–2 applications. • Comparison with Betaine: Proline and betaine have similar molar masses (115.13 vs. 117.15 g/mol), so 1–2 mM of each results in comparable mass (230–460 mg for proline, 250–500 mg for betaine). Their combined effect should stay within autoflower EC limits (1.2–1.8 mS/cm for flowering). Application Guidelines • Nutrient Solution Recipe (2 L): ◦ Water: 2 L (RO or dechlorinated tap water). ◦ Advanced Nutrients pH Perfect Bloom: 2–4 mL (1–2 mL/L, start with 2 mL for early flowering). ◦ Atami Bloombastic: 1 mL (0.5 mL/L, suitable for flowering autoflowers). ◦ Betaine Anhydrous: 250–500 mg (1–2 mM, start with 250 mg). ◦ Proline: 230–460 mg (1–2 mM, start with 230 mg). ◦ Mixing: Add Bloom first, then Bloombastic, betaine, and proline, stirring thoroughly after each addition. Check EC (target 1.2–1.8 mS/cm) and pH (5.5–6.5 for hydro, 6.0–6.5 for soil). • Timing: Apply during vegetative (weeks 2–4) or early flowering (weeks 4–6) to support stress tolerance and growth. Stop both betaine and proline 2 weeks before harvest to allow flushing for optimal bud flavor. • Frequency: Use with each nutrient feeding (e.g., every 2–3 days in hydro, weekly in soil). Alternate or reduce frequency if EC approaches 1.8 mS/cm. • Source: Use L-proline (plant-grade, 99%+ purity) from a reputable supplier (e.g., agricultural or lab-grade sources). Avoid impure or non-plant-specific products. Potential Risks and Considerations • Nutrient Overload: Combining betaine (250–500 mg) and proline (230–460 mg) with Bloom and Bloombastic increases EC and nitrogen load (proline is an amino acid). Monitor for nutrient burn (brown leaf tips, clawing). If symptoms appear, reduce both to 0.5 mM (125 mg betaine, 115 mg proline per 2 L) or skip a feeding. • EC Impact: Betaine and proline each add ~0.1–0.2 mS/cm at 1–2 mM. With Bloom (1–2 mL/L) and Bloombastic (0.5 mL/L) at ~1.0–1.5 mS/cm, the total EC should stay below 1.8 mS/cm. Measure after mixing. • Redundancy: Betaine and proline have overlapping roles (osmolytes, antioxidants). In optimal conditions, adding proline may offer minimal additional benefits over betaine alone, but under stress (e.g., high heat, low humidity), their synergy could enhance resilience. • Cost vs. Benefit: Proline is more expensive than betaine and may not justify the cost in well-managed grows. Test its impact on a small scale first. • Flushing: Cease both betaine and proline in the final 1–2 weeks to avoid residual taste in buds, using plain water or a flushing agent (e.g., Advanced Nutrients Flawless Finish). • Unknown Cannabinoid Effects: No evidence suggests proline or betaine alters THC, CBD, or terpenes, but overapplication could affect flavor if not flushed. Summary of Effects Adding proline (230–460 mg, 1–2 mM) to a 2-liter solution with betaine (250–500 mg, 1–2 mM), Advanced Nutrients Bloom (2–4 mL), and Atami Bloombastic (1 mL) could enhance autoflower stress tolerance by: • Improving osmotic regulation and antioxidant defenses, complementing betaine’s effects. • Supporting photosynthesis and growth under stress (e.g., salinity, heat, low light), potentially increasing biomass or bud yield. • Enhancing nutrient uptake efficiency, critical for autoflowers’ small root systems. Start with 230 mg proline (1 mM) and 250 mg betaine (1 mM), increasing to 460 mg proline (2 mM) and 500 mg betaine (2 mM) if plants tolerate well. Apply during vegetative or early flowering, monitor EC (1.2–1.8 mS/cm), and stop 2 weeks before harvest. Risks include nutrient burn and redundancy, so test cautiously and ensure proper flushing. If you provide your grow setup (e.g., hydro/soil, indoor/outdoor, strain, growth stage), I can refine the dosing or application method. Let me know if you need sourcing tips for proline or further guidance!”” 7/27 3PM. Adding **0.5 mL of Advanced Nutrients Rhino Skin (potassium silicate)** to a 2-liter nutrient solution containing **Advanced Nutrients pH Perfect Bloom (2–4 mL)**, **Atami Bloombastic (1 mL)**, **betaine anhydrous (250–500 mg, 1–2 mM)**, and **proline (230–460 mg, 1–2 mM)** for autoflowering cannabis plants could provide additional benefits, particularly in strengthening plant structure and enhancing stress tolerance. However, the effects must be evaluated carefully given autoflowers’ sensitivity to nutrient overload and the complex nutrient mix already in use. Below, I analyze the potential effects of adding 0.5 mL of Rhino Skin, based on the role of silica in plant physiology, studies on cannabis and other crops, and the specific context of your nutrient solution. ### **Background on Silica (Advanced Nutrients Rhino Skin)** - **What is Rhino Skin?**: Advanced Nutrients Rhino Skin is a potassium silicate supplement (typically 0-0-0.4 N-P-K, with ~4–7% silica as SiO₂). Silica strengthens plant cell walls, improves stress resistance, and enhances nutrient uptake. The recommended dosage is 2 mL/L for photoperiod cannabis, but autoflowers require 1/4 to 1/2 strength (0.5–1 mL/L) due to their sensitivity. - **Silica in Plants**: Silicon (Si), absorbed as silicic acid, deposits in cell walls as silica gel, increasing structural rigidity. It also mitigates abiotic (e.g., drought, heat) and biotic (e.g., pests, fungi) stresses and can improve photosynthesis and yield. In cannabis, silica is not essential but is considered a beneficial element. - **Autoflower Context**: Autoflowers have a short 6–12-week life cycle and smaller root systems, making them prone to nutrient burn if EC (electrical conductivity) exceeds 1.2–1.8 mS/cm during flowering. Rhino Skin’s low dosage (0.5 mL per 2 L, or 0.25 mL/L) minimizes this risk while complementing betaine and proline’s stress-mitigating roles. ### **Potential Effects of Adding 0.5 mL Rhino Skin** Based on silica’s effects in cannabis and other plants (e.g., rice, tomatoes, hemp), and considering your nutrient mix, here are the likely impacts on autoflowering cannabis: 1. **Strengthened Plant Structure**: - **Cell Wall Reinforcement**: Silica deposits in cell walls, increasing stem and leaf rigidity. In hemp (*Cannabis sativa*), silica supplementation (e.g., 1–2 mM Si) thickened stems and improved mechanical strength. For autoflowers, which are compact (1–3 feet tall), 0.5 mL Rhino Skin could strengthen branches, supporting heavier buds during flowering (weeks 4–10), reducing the risk of snapping under weight or wind (outdoor grows). - **Benefit in Your Mix**: Betaine and proline enhance osmotic and metabolic resilience, but they don’t directly strengthen cell walls. Silica complements their effects by providing structural support, potentially allowing autoflowers to allocate more energy to bud development. 2. **Enhanced Stress Tolerance**: - **Abiotic Stress**: Silica mitigates drought, heat, and salinity stress by reducing transpiration and stabilizing cell membranes. In tomatoes, 1 mM silica improved water retention under drought. For autoflowers, 0.5 mL Rhino Skin (~0.1–0.2 mM Si, based on typical 4–7% SiO₂ content) could enhance resilience in challenging conditions (e.g., low humidity, high temperatures, or high EC from Bloom/Bloombastic). - **Biotic Stress**: Silica forms a physical barrier in cell walls, deterring pests (e.g., spider mites) and pathogens (e.g., powdery mildew). In cannabis, silica reduced fungal infections in controlled studies. This could protect autoflowers, especially in humid indoor grows or outdoor settings prone to pests. - **Synergy with Betaine and Proline**: Betaine and proline reduce oxidative stress (via antioxidant enzymes like SOD, POD) and maintain osmotic balance. Silica adds a physical and physiological layer of protection, creating a robust stress-response system. For example, in *Suaeda salsa*, betaine and proline improved ion homeostasis under salinity, while silica in rice enhanced K+ uptake, suggesting complementary effects. 3. **Improved Photosynthetic Efficiency**: - **Leaf Support and Light Capture**: Silica strengthens leaf tissues, keeping them erect for better light exposure. In rice, 1.5 mM silica increased chlorophyll content and photosynthetic rates. For autoflowers under high-intensity lights (18–24 hours), 0.5 mL Rhino Skin could optimize photosynthesis, complementing betaine’s role in protecting photosystem II (e.g., upregulation of Capsb genes in peppers). - **Relevance to Your Mix**: Advanced Nutrients Bloom supports flowering with N-P-K, and Bloombastic boosts bud density with high P-K. Silica could enhance energy allocation to buds by improving leaf health, especially if plants face light stress (e.g., inconsistent outdoor light). 4. **Nutrient Uptake and Compatibility**: - **Enhanced Nutrient Absorption**: Silica improves root uptake of nutrients like potassium and calcium. In maize, 1 mM silica increased K+ and Ca²⁺ levels. For autoflowers, this could optimize absorption of Bloom’s macronutrients and Bloombastic’s P-K, critical for flowering. - **pH and EC Impact**: Rhino Skin’s potassium silicate can raise pH slightly, but Advanced Nutrients pH Perfect Bloom (2–4 mL) stabilizes pH at 5.5–6.5 (hydro) or 6.0–6.5 (soil). At 0.5 mL per 2 L (0.25 mL/L), Rhino Skin adds minimal EC (~0.05–0.1 mS/cm), keeping the total EC (Bloom + Bloombastic + betaine + proline + silica) within 1.2–1.8 mS/cm for flowering autoflowers. - **Compatibility with Betaine and Proline**: Betaine (1–2 mM) and proline (1–2 mM) are neutral organic compounds that don’t chemically interact with silica (an inorganic compound). Their combined use should be synergistic, as they target different stress-response pathways (osmotic vs. structural). 5. **Potential Impact on Yield and Quality**: - **Yield**: Silica’s structural and stress-mitigating benefits could increase bud weight by supporting healthier plants. In hemp, 1 mM silica increased biomass by 10–15%. For autoflowers (28–112 g/plant indoors), 0.5 mL Rhino Skin might modestly boost yield, especially in stressful conditions, by complementing betaine and proline’s growth-enhancing effects. - **Cannabinoid/Terpene Profile**: No direct evidence links silica to THC, CBD, or terpene changes in cannabis, but stronger plants may produce denser buds or more resin. Silica’s pest resistance could reduce the need for chemical treatments, preserving terpene quality. Proper flushing remains critical to avoid flavor impacts. ### **Recommended Dosage and Application** - **Dosage**: **0.5 mL Rhino Skin per 2 L (0.25 mL/L)** is optimal. - **Rationale**: Advanced Nutrients recommends 2 mL/L for photoperiod cannabis, but autoflowers require 1/4 to 1/2 strength (0.5–1 mL/L). At 0.5 mL per 2 L, Rhino Skin provides ~0.1–0.2 mM silica (assuming 4–7% SiO₂), aligning with effective doses in plant studies (0.5–2 mM) while minimizing EC and pH impacts. - **Confirmation**: Your specified 0.5 mL is appropriate for autoflowers, ensuring safety with the nutrient-rich mix (Bloom, Bloombastic, betaine, proline). - **Nutrient Solution Recipe (2 L)**: - **Water**: 2 L (RO or dechlorinated tap water). - **Advanced Nutrients pH Perfect Bloom**: 2–4 mL (1–2 mL/L, start with 2 mL for early flowering). - **Atami Bloombastic**: 1 mL (0.5 mL/L, suitable for flowering). - **Betaine Anhydrous**: 250–500 mg (1–2 mM, start with 250 mg). - **Proline**: 230–460 mg (1–2 mM, start with 230 mg). - **Advanced Nutrients Rhino Skin**: 0.5 mL (0.25 mL/L). - **Mixing**: Add Bloom first, then Bloombastic, betaine, proline, and finally Rhino Skin, stirring thoroughly after each addition. Check EC (target 1.2–1.8 mS/cm) and pH (5.5–6.5 for hydro, 6.0–6.5 for soil). - **Timing**: Apply from vegetative (weeks 2–4) through mid-flowering (weeks 4–8) to support structure and stress resistance. Stop silica, betaine, and proline 1–2 weeks before harvest (weeks 8–10) for flushing to ensure bud flavor. - **Frequency**: Use with each nutrient feeding (every 2–3 days in hydro, weekly in soil). If EC nears 1.8 mS/cm, reduce Bloom to 2 mL or alternate silica applications. ### **Potential Risks and Considerations** - **Nutrient Overload**: The mix (Bloom + Bloombastic + betaine + proline + silica) is nutrient-dense. At 0.5 mL, Rhino Skin adds minimal EC (~0.05–0.1 mS/cm), but the total EC (1.2–1.8 mS/cm) must be monitored to avoid burn (brown leaf tips, clawing). If symptoms appear, reduce Bloom to 2 mL or skip silica for one feeding. - **pH Stability**: Rhino Skin’s potassium silicate may slightly raise pH, but pH Perfect Bloom should counteract this. Verify pH after mixing (5.5–6.5 for hydro, 6.0–6.5 for soil). - **Redundancy**: Betaine, proline, and silica all enhance stress tolerance (osmotic, metabolic, structural). In optimal conditions (e.g., indoor, 70–80°F, 40–60% humidity), silica’s benefits may be marginal, but under stress (e.g., heat, pests), it adds a complementary layer of protection. - **Flushing**: Stop all additives (silica, betaine, proline) 1–2 weeks before harvest, using plain water or Advanced Nutrients Flawless Finish to avoid residual taste in buds. - **Cost vs. Benefit**: Rhino Skin is relatively affordable, but its benefits may be less pronounced in well-managed grows. Test on a few plants to assess impact. - **Cannabinoid Effects**: No evidence suggests silica alters THC, CBD, or terpenes, but improved plant health could enhance resin production. Overuse risks silica deposits affecting flavor if not flushed. ### **Summary of Effects** Adding **0.5 mL Advanced Nutrients Rhino Skin** to a 2-liter solution with **Bloom (2–4 mL)**, **Bloombastic (1 mL)**, **betaine (250–500 mg)**, and **proline (230–460 mg)** could: - Strengthen stems and leaves, supporting heavier buds. - Enhance resistance to abiotic (drought, heat) and biotic (pests, fungi) stresses. - Improve photosynthetic efficiency and nutrient uptake, complementing betaine and proline’s osmotic and metabolic benefits. - Potentially increase yield (28–112 g/plant) by reducing stress setbacks. The 0.5 mL dose (0.25 mL/L) is safe for autoflowers, keeping EC within 1.2–1.8 mS/cm. Apply during vegetative to mid-flowering, stop 1–2 weeks before harvest, and monitor for nutrient burn. The synergy of silica (structural), betaine (osmotic), and proline (metabolic) could optimize autoflower health, especially under stress. If you share your grow setup (e.g., hydro/soil, indoor/outdoor, strain, growth stage), I can refine dosing or application further. Let me know if you need sourcing tips or additional guidance!