Day 22: Drain EC 3 feet high and risin'😅( average 3.3 to be exact...) dunno how much whatever-salt is building up in that pots, but it obviuosly doesn't bother the ladies. Dark green colour, very few 'claws', no signs of tipp-burn, new foliage appears in a juicy green and turns dark in an instant, leafs point upwards....... looks like they're happy😊. Planns for this week: - Install the scrog-net - Obsereve plants/soil ( when do i have to feed nutrients again?) otherwise there will be no changes...... Thoughts: These bushy babies will be kind of a challlenge to create this scrog the way i'm used to (basically bending the 4 main main colas to the corners of the tent, upper branches to the walls and spaces between the pants, lower branches to the very center). Now these ladies make so many branches, so close togehter and so equal in length, underneath huge fanleafs..... i'd love to have them in a 100 square meter canna-crib....but in a tent...????? I really hate doin' things without having them planned - but in this case i guess i will have to install the net, start weaving and see what's happening😯 Day 23 Let's keep it positive...... indoor-climate of 64% rh is perfect for my health💪😁!!! ......but in my tent it's killing the vpd😖. at a lousy 24 degrees C, i'm readin' about 60-64 % rh wich results in about 0.7 to 0.8 kPa.* More figures? Here come the crazyones👻!: pH 5.5; EC 4.2😨 but thats only drain from plant 3....... average was 6.1/3.6 The girls busy produce roots, shoots and leafs....... still very green, still not unhealthy.......few claws, no tipp-burn. Basically a comfortable place to be: It's not bad yet - no work but watering - and it can only get better!!!😁 *VPD issue solved: The sensor hung too close to a fanleaf - didn‘t expect this thing beeing so sensitive 😅. Day 24 Climbed new hights this morning: Plant 1 had an EC of 3.8 - the others didn't climb any higher than yesterday. So maybe we've reached the climax..... 😇 Visible growth now - so i installed the scrog-net and will start bending and weaving these days:-) Thoughts: Did beeing out of VPD-range for one day harm my plants? How exact can my sensor measure VPD? after a bit of research i found out: Whatever you buy for less than 200 bucks is far from beeing exact! Even professional sensors have a margin of about +/- 1% in relative humidity. Thermometers at least +/- 0.5 degrees C. This could give some a good guess of the actual VPD BUT: Leaf-temperature-offset is absolute key! Many of our trusted homegrow-equippment isn't even able to measure it (some offer default temp-offset) ....don't wanna go further here - but there's no way to achieve something like a precise VPD-measurement in homegrowing. So why caring for VPD? Even with those less accurate instruments, you may be able to keep your plants in something like a comfort-zone. Way better than only guessing 👆!!! In case i ran a much higher VPD than planned, yesterday (due to a missplaced sensor) , it will not harm or kill my plants. they might have had a bit of stress from dry air - but nothing to worry about! STAY TUNED 👈!

Questa settimana ho deciso di montare una lampada di supporto a LED per poter far arrivare a tutte le piante abbastanza luce, le piante continuano a crescere e i fiori sembra che stiano maturando per bene spero di raccogliere almeno 4/5 piante entro 2/3 settimane,perché alcune sono molto indietro rispetto ad altre.

Hi guys, Welcome back to Queen Peaky's Grasslands We're into week two of rinsing off these gorgeous little girls covered in strawberry Frosting You can already perceive some excellent smells👻 Follow us and always support us to always be on top Kiss kiss Peakyplanter La Reina

I was very shocked on how fast it grewand how much it produced very great strain a must for you Indica lovers very strong I went to about 80% red hairs perfect smoke a lot of cannabinoids and a lot of leftover sugar leaves great for lovers of bubble hash

Day 2 - LST and topping/mainlining where possible before flowering Both plants have broad leaves blocking light to the canopy

Bueno familia terminamos la 4° semana y damos comiendo a la 5° lo que pensaba que era los nutrientes eran quemaduras del viento y la luz ahora lo e acomodado y creo que van mejor están engordando bien y ya sueltan un olorsito rico 😁😁como siempre familia cualquier ayuda cualquier Consejo bienvenidos sean un abrazo familia y buenos humos paz

Me encanta la estructura conseguida en estas plantas. Ramas robustas y nudos cortos. Se viene una buena prefloración. Un saludo GanjahFamily.💚

Love Glookies, Glookies is loving the ScrOG ;) super vigorous plant, that handled and repaired more than a few broken branches amazingly. The pheno is awesome, with buds being light green with a ton of purple hues in them. A powerhouse that I can highly recommend. Took a bunch of clones so that I can grow her again right away. She is a stretcher so beware and factor that in when growing her.

Colour coming through

Week 10 their wasn’t any real changes. Girl #1 starting to gain more weight. Her smell is also increasing. She smells like citrus/ pineapples undertones. Girl #2 still hasn’t shown any flowering. It’s been 70 days and I’m starting to get worried. I’m gonna let her go until girl #1 finishes than I will start to panic. I fear she may be a 100 day to flower pheno . If nothing changes in the coming weeks I may treat her like a photo or just get rid of her !? Thank you for viewing my diary.

Hey guys, late into week 6. We had a wind storm roll through and thrashed the crap out of the ladies, luckily nothing broken or bent thankfully. I was quite worried because they where droopy gor a few days after but I notice they started to recover on thier own. They are still not 100% yet. Other than that last time I watered was Sunday. It's has been 5 days and the pots dried out nicely. Watered them with 50mL of molassas in 5gal of water pH to 6.1. Topped the tallest branches to help the lower canopy to catch up. It's been 2 weeks and each branch I topped already have 4-5 nodes. Perfect timing. Thanks for reading.

Popped easy, then put into Coco Coir/Organic Perlite Mix and used CropSalts Veg A Veg B and Run Clean. Ran 5.8ph and 800 EC as Coco was pre buffered and first run off was over 1300. Adjusted well then adjusted EC to 900. Runoff was still equally out before transplant.

The girls loved these past sunny two days but tomorrow we have rain again. Around the weekend we Will have alot of sun! Yet another day of rain so the girls are once again under a roof outside. Sun is coming the say but they need it now! cheers! Another rainy day tomorrow

We put 40ml of pure water and we add 5 drops of oxygen water and then put the seeds

Week 8 - Dead line is coming, starting week 8 of flowering. The routine is maintained, feeding only with water. The pistils take on an orange color. I think Gorilla Girl will pass 10 weeks of flowering.

10/13/2018 #1 still looking good, no fade. Start of week 9 of flower hope buds put on some weight this week should be 1 week maybe 2? Gonna be cold mid- week 38° on two nights. I know they can handle lower 40's without any problems but we will see in next weeks update,a cold tolerant sativa leaning girl is welcome hereCanfy #4 sweet fruit,and smelly shoes

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.