Tous ce passe bien merci à tous pour votre soutien .

This week was great aside from my first sour d fell off the table and I lost the seedling. This is my second go, no paper towel germ this time, directly in soil

Como estáis fumetillas, yo estoy flipando, y es que las flores de estas skunks son como rocas, y no solo lo compactas que están si no que están bastante tricomadas, las 3 a cada cual más , lavamos las raíces y ya estarán solo agua, dejemos que termine de florecer y madurar, tampoco hay prisa, ph controlado temperatura ideal, bajamos algo más la humedad, puede que tengamos que tuitear alguna pero estoy evitándolo, se irá viendo estos días, ya nos veremos esta semana que viene un saludo familia y buenos humos.

Solo un riego mas con abono y hago lavado de raíces..:D

This week I've been pulling the top fan leaves to stop the plant growing taller and to allow the side branches to catch up. I also started feeding them. I will probably repot and switch to flower next week now they've all outgrown their mutations.

👉DIA : 13/12/21👈 Metodo de germinacion: 👉<< VASO DE AGUA >>👈 seleccionamos las cantidades deseadas de semillas y en un pequeño vaso con agua opmotizada insertamos las semillas para nutrirlas durante 12H, situada en un lugar sin luz, apropiadamente tapada con un trapo.😉 __________________________________________________________________________________________________________________________________________________________________________________ 👉DIA : 14/12/2021👈 👇👇👇👇👇👇👇👇👇👇👇👇 una vez pasada las 12 H de germinacion en vaso de agua , depositamos las semillas bien colocadas en un recipiente con papel de cocina humedecido con agua y 1ML de estimulador de raices (TOPCROP). Esperamos de 1 a 2 días a que salga la raiz de la semilla y ser transportada a los Jiffy😉 ___________________________________________________________________________________________________________________________________________________________________________________ 👉DIA 17/12/2021👈 👇👇👇👇👇👇👇👇👇👇👇👇 Pues pasados los 3 dias de germinacion en el metodo de papel, vemos que ya salieron a luz las raices y damos paso a colocarlas en los Jiffy. 👉Instrucciones:👈 - Colocación de los jiffy en un recipiente con 2L de agua y 2ML de enraizantes de top Crop. - Dejamos actuar durantes varios minutos hasta que el jiffy se extienda. - Abrimos un pequeño orificio en los Jiffy para introducir la semilla con el cáliz dejandolo hacia afuera. - Colocamos en un pequeño invernadero para que tengan la suficiente humedad. - Ayudandonos de unos foco de pocos waltios, le damos a luz necesaria para esta face de germinacion dejandolas 24 horas de luz. ________________________________________________________________________________________________________________________________________________________________ DIA 22/12/2021 Colocacion de Jiffy en Maceta de 3,25L 👇👇👇👇👇👇👇👇👇👇👇👇 👉Instrucciones👈 -Pasados varios dias vemos que el periantio(semilla) ya se rompió y empezo a salir los cotiledones con una hermosa raiz blanca por debajo del jiffy. -rellenamos macetas de 3,25L con sustrato de top crop(anterior cultivo). -introducimos los jiffy en las macetas dejando la plantula bien colocada. -regamos con 3L agua opmotizada y 1ML/L enraizante Voodoo ADVANCED NUTRIENTS - Permanecemos con las 24 horas de luz con foco led. _________________________________________________________________________________________________________________________________________________ 👉DIA 25/12/2021👈 👇👇👇👇👇👇👇👇👇👇👇👇👇👇👇 pasados 3 dias desde que se plantó en maceta. A dado unas respuestas increibles !!👍 las volvemos a regar y a cambiar las horas de luz que se pasarian a 18H

Le stretch est vraiment ridicule, je voulais que ça monte jusqu'en haut... Tant pis... En tout cas la flo est lancée 😁 Dans l'ordre: 1) Afghan Peach x Blue Monkey 2) Gelato Cake 3) Fast Critical Poison 4) Tropical Fuel 5) Hindu Kush 6) (Blueberry x Black Domina) X (Kosher Kush x Mk-Ultra) 7) Blueberry 8) Herz OG 9) (Blueberry x Black Domina) X (Kosher Kush x Mk-Ultra)

after 10days in the 12/12 light cycle we lollipopped our plants a little bit. 3 days later we were shocked: the supa choopz was hermaphroditic...What an unfortunate thing considering the good genetics of zmoothiez. We sorted her out, luckily we still have a clone in reserve and he will now make use of the free space. The clone only had one week of veggie in the tent then it was sent into flowering, it has developed magnificently and is as big or bigger than the other ladies but unfortunately not quite as strong. We are still curious and hope for no further unpleasant surprises.

08/25: 5ème semaine premier jours il ne reste plus grand chose à faire à part arroser avant le rinçage 😎🤗🤗😎 #56 08/26: flushing RO: 440ppm #57 08/27: we must wait t'il end of september until I smoke my first firecracker of this O.S. Life is so ruff with me. :D #58 08/30: prochain flush dans les jours merci @RastoMouse for the help canna flush. @growdiaries.com #61

Info : Unfortunately, I had to find out that my account is used for fake pages in social media. I am only active here on growdiaries. I am not on facebook instagram twitter etc All accounts except this one are fake. Flowering day 16 since the time change to 12/12 h. Hey guys :-) Like every week, at the beginning I can only mention how extreme it has grown again :-). The network is perfectly filled. Next week I will remove everything that grows under the net so that the full energy goes into the upper area. At the mix from Canna Aqua Vega was left out of fresh water this time, but Canna Aqua Flores was increased. The plant has been thoroughly examined and it is all in Green area 👍. The buds should start developing next week. Until then, I wish you all a lot of fun with the update. Have a good start to the week everyone. Stay healthy 🙏🏻 You can buy this Strain at www.Zamnesia.com Type: Runtz ☝️🏼 Genetics: Zkittlez X Gelato 👍 Vega lamp: 2 x Todogrow LED CXB3590 COB 55 W 1 x Sanlight S2W 62 W 💡 Flower lamp : 2 x Todogrow LED CXB3590 COB 55 W 1 x Sanlight S2W 62 W 💡 ☝️ Grow Aero System : Growtool 0.8 ☝️ Fertilizer: Canna Aqua Vega A + B , Canna Aqua Flores A + B , Rizotonic, Cannazym, CANNA Boost, Pk 13/14, Canna Cal / Mag, Canna Ph - Grow, Canna Ph-Bloom ☝️🌱 Water: Osmosis water mixed with normal water (24 hours stale that the chlorine evaporates) to 0.2 EG. Add Cal / Mag to 0.4 Ec Ph with ph- to 5.2 - 5.8 💦 💧

Al final de la jornada realizamos poda apical

hi to all, brothers and masters of weed. my queens are really strong !!! I started with the flushing for these little girls who have started to turn purple .... the smell is very strong and it is really AMAZING The buds are literally covered with powerful glue that just touching them release a strong and pleasant smell .... I think I will not throw anything from the waste because every corner of the plant is covered with sparkling and powerful trichomes with which I hope to make good hashish I leave you wishing you a happy and abundant harvest for all of you see you around

Watered in coconut water, microbes & aloe. Topdressed some bloom Fan fell on front left plant when I was moving it broke a top lol. Whoops

Wow die Streckung war extrem aber bin voll auf begeistert von der 24K liefert bis jetzt eine top Performance ab diese Woche hab ich einmalig PK 13/14 hinzugefügt

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

10/16: She is basically already done. I changed out the nutrients for water and Flawless Finish today. She is now flushing. I have been so surprised by this plant. She entered flower and finished quicker than i expected. I will be harvesting her this time next week. I am not blown away by the smell. i expected it to be more fruiting and sweet, but it just smells mostly earthy at this point.

29 May 2021 I have been super busy and have neglected my little girls the last few days. I started the flowering stage (12/12 light schedule) yesterday, the 28th. I was going to start it on the 26th, but had trouble with the timer. I’m going to stick to my schedule though. Today starts the flowering stage feeding chart, and for this week I’m using the “Transition to Flower” amounts. The water was chilled to 41.5 degrees F, and I began feeding an entire gallon as well. I fed: CALiMAGic 5ml, FloraMicro 7.5ml, FloraGro 7.5ml, and FloraBloom 7.5ml. The initial pH was 6.7 and needed no adjustment. The TDS going in was 851 ppm. Runoff pH was 6.6, and TDS runoff was 1885 ppm. Tent temp was 75 degrees F, and humidity was 50%. I defoliated the living hell out of her today, and rearranged some of her LST points. I also cut some of the lower branches/stems to make the growth more controlled. I have enough to make several clones if I decide to do that. I’m still trying to make up my mind on that. 31 May 2021 My Blueberry Muffin is looking fabulous!! She has adjusted to her massive defoliation with ease and is looking BEAUTIFUL! I’m very pleased with how she’s doing. Both she and her tent mate are looking wonderful. I’m still using the “Transition to Flower” stage, so here’s what I fed: 1 gallon of water chilled to 51.2 degrees F, with CALiMAGic 5ml, FloraMicro 7.5ml, FloraGro 7.5ml, and FloraBloom 7.5ml. The initial pH was 6.7 and didn’t need to be adjusted. The TDS going in was 1054 ppm. She took the whole gallon and had plenty of runoff. The runoff pH was 6.5, and TDS runoff was 2237 ppm. Tent temp was 79 degrees F, and humidity was 44%.

Not having any problems except a few bugs not enough to worry about.