Only watered with water this week and runoff ppm still shows around 1000. There is no burning so it seems Bio tabs did their job. Removed the SCROG at the end of this week in preparation for harvest. Harvest will be tommorow at day 176 ( 71 from flower) Trichosomes seem around 50% cloudy with around 25% amber and 25% clear. Chopped her Day 71 from flower, completed budwash with 3 buckets solutions using filtered water and lemon juice, soda b and , hydrogen peroxid 3%, only using very low amount compared to Doc Budwash tehnique found on forums cause I didn't have more then 250 mL at home. Weight is around 2.5 KG wet before washing. Now trying to achieve stable humidity around 50%, 60 % and planning to start trimming i na few days....

Plantulas mostrando un buen enraizamiento. Se preparo una solucion de Elite 91 Myco Jordan iniciando la semana con: -PPM : 80 -pH : 5.8 3/7/2022 se transplanto a cubos de lana de roca de 15cm x 15cm. Los cubos fueron submergidos bajo una solucion de Mills Start R con: PPM : 100 pH: 5.7 En el transplante se uso Elite 91 Myco Jordan en forma de polvo para cubrir el hueco del cubo y cubrir las raizes de las plantulas para reducir el estres del transplante.

Ja die 4/5 hat angebrochen, die Pflanze wächst gut und die buds werden immer praller :) die Pflanze wurde auch sehr frostig! Und wenn wir schon bei den terpenen sind, sie riecht sehr süß mit einer Holz Note dabei das macht richtig Bock das Zelt zu öffnen. Dafür das die Pflanze die kleinste is riecht die mehr als die Strawberry und Cream Caramel. Ich hoffe aber sehr das die Pflanze keine Bananen bekommt wie die strawberry ich werde beide Pflanzen die übrig sind ab jetzt mehr kontrollieren müssen.

I said!! it's huge ready for flowering!! one week to change the light schedule to 12/12 I've already lost count of the toppings I've made. I will always take some of the bottom leaves out. that no longer catch light and only waste energy. I water every two days. small quantities and One feeding per week. I make adjustments whenever necessary. I also don't spend a lot of nuts as long as she doesn't really need food as in flowering. you will realize soon

Some rainy days and some sunny days this week. Warm temperature but not too hot. I started to look at the trichomes and they begin to turn cloudy. Some big leaves start to turn yellow.

Hi growmies, this week the twins were not getting any sunlight at all so they had to keep each other warm in their clubhouse😸 now they are on my gardentable. to catch the few sunrays .. Peeking through the clouds.. One in 11 litr pot is 43cm 5L pot is 69 cm 5L- strechting like a beast.. she still looks like a big freak with her deformed leaves but i feel all she needs is just a few days of good sunlight (she is a bit 'droopy') and she will make buds like a beast.. i feel it could go either way or she will be a big bust or shes gonna make the biggest buds ever! 11 l pot stands strong and trying to take care of the hedgehogs as good as possible has bigger trunck and is much more sturdy.. she wil not dissapoint😎👌 They are getting a little bit attacked by spiders and spidermites in their clubhouse.. but thats no biggy few snails too.. but they are big enough to not get bothered by them and usually i catch them in their tracks (both, the spiders and the snails ;)) ihave only found one 'lil snail in my cheese..but have done no harm, just dazed from the cheese smell i think 😎👌 think gonna put some copper tape around the pot, just to be sure no snail invasion comes becouse it is so rainy.. but im just glad there are no caterpillers (yet) they can really munch on the plants! see ya next week with another episode of the Cheese Twins Chronicles hopefully in full sunlight! happy growing for all✊

Update coming.

Day 101. 22 hour dark period. Day 102 chop and hang.

Three at my place and they are purple as can be! Gonna finish up beautifully in the next weeks. If I didn’t put these girls down they would be standing at least 60” tall. Insane how big these autos are. These purple ones in my tent are entering week 4. The other three out of the same pack are at my dads under my hps set up. These girls are about 50” just early on in flower

looking lovely. Although they still seem small. Not sure how long they will take to finish. They will probably shoot up once they get into the large air pots. Pretty happy with the progress so far though. Meaty main stems. Humidity isn't quite as high as I would like but it's a fair compromise with others flowering. As for superthrive. Does it work? Well I'm not seeing the explosive growth it promised in the root zone. But it's not really a fair trial as they've all had it.

Estamos en la última semana para dos de las cuatro variedades, así que a partir de este momento, eliminamos Bio Flores. Añadiremos al bidón de riego Canna Flush, que nos va a ayudar a lavar bien nuestras raíces, eliminando restos de sales y nutrientes. Buscamos que las plantas gasten sus reservas de las hojas, que poco a poco veremos como pierden verdor, eliminando clorofila, sobre todo las más bajas. Canna Flush - 20ml por cada 10 litros de agua. Bajamos la potencia de nuestros balastros a 600w.

Well I'm a bit upset last week I ended up dropping my ph meter in a tank of nutes I was mixing and by the time I got it out the meter was junk. I was planning on flushing the crystal but had to wait another week for the meter. I suppose the good thing to take from this was I found out why I was having issues with my plants. When the new ph meter came in I went to check my run off and my nutes that I had all ready mixed up. It turns out that my meter i had been using this whole grow was off by about -2-2.5 so this whole time I had been feeding the plants at around 3ph smfh. I cant believe they look how they do knowing what went on this whole time. Fastbuds crystal meth is 3days into flush and i will be cutting it down this weekend most likely. Mephisto hubbabubbasmelloscope is smelling like sweet sweet candy and is thick in resin Bighead seeds freeze berry/ blueberry crumble has a dank pungent stank to it and also is frosty but larrfy Big bomb is still growing thick and have bud sites everywhere Now I'm ready for the next batch and ready to get dialed in!

Will put final dry weight numbers up. I’m expecting around 6oz

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Hello everyone friends farm Welcome Back ! We lowered this beautiful girl's apex peak!

ultimi giorni gli sto facendo prendere più luce spero tiri fuori tt gli zuccheri possibile 💪

this plant surprised me, its flowers became dense and heavy, much more than its mother's. The yield was much better than I imagined. I am really satisfied. I had never cloned an autoflowering plant and that sounds cool.

🍬 she finicky to dail in hope in the future I can really dail her in and get pheno a buds of the gushers cake lookin perfect pheno b of the gushers cake looks good tho

💚💜 HULKBERRY 💜💚 28.6.25: The plant was watered with 2ltrs of dechlorinated water PH'd to 6.6 containing: ♡ 1/2 TSP Cal-Mag PH: 6.6 PPM: 1060 Pus an additional 2ltrs of dechlorinated water PH'd to 6.6 containing the following nutrients; ♡ 1/2 TSP Bud Explosion ♡ 1/4 TSP Sea K(elp) PH: 6.6 PPM: 1170 29.6.25: I watered with 2ltrs of dechlorinated water PH'd 6.5 with; ♡ 1 TSP Cal-Mag. PH: 6.5 PPM: 1060. 3.6.25: The plant was watered with 2ltrs of dechlorinated water PH'd to 5.9 containing the following nutrients; ♡ 1/2 TSP Cal-Mag PH: 5.9 PPM: 1070 Plus an additional 2ltrs of dechlorinated water PH'd to 5.9 containing the following nutrients; ♡ 1/4 TSP Sea K(elp) ♡ 1/4 TSP Bud Explosion PH: 5.9 PPM: 1060 4.6.25: I watered with 2ltrs of dechlorinated water PH'd to 6.0 with the following nutrients; ♡ 1/2 TSP Mega Crop Part A PH: 6.0 PPM: 1090 Plus an additional 2ltrs of dechlorinated water PH'd to 6.0 with the following nutrients;. ♡ 1 TSP Sea K(elp) ♡ 1/4 TSP Bud Explosion PH: 6.0 PPM: 1050 6.6.25: The plant was watered with 2ltrs of dechlorinated water PH'd to 6.3 with 1/2 TSP Cal-Mag. PH: 6.3 PPM: 1470 Plus an additional 2ltrs of dechlorinated water PH'd to 5.9 containing; ♡ 1/2 TSP Bud Explosion ♡ 1/2 TSP Sea K(elp) PH: 5.9 PPM: 1160. We are still going through a large amount of water, and I was really planning on chopping her last week. The underdeveloped buds are beginning to ripen up slowly, so I'm going to keep her another week and see where we are then. Many thanks for checking out this week and hanging out in the comments 💚🙌🍃😊🌱✌️