07 august 2018, thick buds are al;ost ready for harvest. Will let it for couple weeks. Today gonna stop using Plagron and pass to clear water.

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End of week 7. Great week. Big plants. Smell is so strong, diesel and incredibly powerful turpentine. Really fast Strain to grown. Flushing started, should be a big harvest when done. Thanks everyone, I show my grows on YouTube also, Crystal Garden

My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems.

😶‍🌫️😶‍🌫️👏🏻👏🏻👏🏻

Predators Unleashed & First Frost ❄️🕷️ This week brought a few challenges—but also some exciting signs of progress! First off: spider mites tried to crash the party, but I wasn’t having it. 😤 I’ve been manually removing them every day, and today—finally—no new sightings. On top of that, I released a fresh squad of predatory mites, who are now keeping things in balance. Nature's tiny enforcers are on the case. 🕵️‍♂️🔬 I noticed some slightly yellowing lower leaves, so I trimmed them off. It could be a touch of nitrogen deficiency, so I slightly increased the BioBizz Grow dose during this week’s feeding. I’ll keep a close eye on it over the coming days. 🌿 Otherwise, she’s looking strong, vibrant, and full of life. Beautiful structure, steady growth, and she’s just starting to shift into bloom mode. I gave her the usual round of care: 💧 Weekly care routine: 🔸 BioBizz Grow, Bloom, TopMax, CalMag & Alg-a-Mic 🔸 Compost tea (homebrewed magic, as always) 🔸 Effective Microorganisms for a living soil boost 🔸 And this morning—a full feeding session to keep things on track ✨ Exciting signs of change: The first trichomes are starting to sparkle under the sun, and when I sit next to her, there’s already a faint, sweet scent in the air. She’s officially getting into bloom mode! 😍 Now that she’s out of the tent, she’s following the natural 16/8 rhythm—and since she was hormonally triggered into flower before leaving the tent, she’s holding that bloom energy steady outside. 🌞🌒 The vibes are good, the buds are on the way, and I couldn’t be more excited to see where this beauty goes next. Let the Red Hot journey continue! 🔥💚

Muy buena cepa, potente, productora y fácil de cultivar. El efecto fue exactamente lo que buscaba; intenté curar la yerba lo mejor que pude para poder preservar los terpenos y así disfrutar aún más. Cepa recomendada😄

Day 14 Veggie Still in propagation tent; the back two rooted nicely, looks like the air stone was pushed back so they got the most benefit. I still need to clean out the veg tent for transplant into hydroton and 2 month grow period. 🎼🎶 I was gonna clean out the grow tent, but then I got high...🎵 JK.... I’m lazy.

Una vez pasados los dias huele bién, sabe bién y buen efecto. Matices y aromas Terrosos , café y a cítricos. Fácil de cultivar.

Taking a heavy heat wave in my area... so my girls are beeing cooked alive I hate to see them like this... I already added more CO2 to help them compensate a bit. Well the clones are beeing flushed for almost 5 days now in 4-6 days im gonna put them into 48 h darkness and harvest wish me the best =) Trichomes are looking evenly brownish all over the plants now just like I love them :P Also trying out the Clean fruits for flushing will see what it can do for me=)

Aug 30: still hot. Sep 2: heat wave is now past and we’re now into a couple days of smoke. Cut back water volume from 9 L/day when it was hot to 5-6 L lately. Sep 5: don’t take flash pics of photoperiod plants unless you follow it up with a red light.

Still pre flower in my area I suspect it will start to bloom in three weeks or less

Muy olorosa genética, me encantó mucho, termino de engordar al 100% los cálices absorbiendo los postilos de las flores y eso para mi es muy importante, muy agradable al fumar con un efecto inmediato apto para el día a mi pensar ya que su efecto se nota mucho pero no es para dejarte tumbado si no que con mucho ánimo en fin muy agradable para conectar tu mente a la música que es algo que me encanta al fumar, en fin muy fácil de cultivar, crece mucho así que procura dar poca vegetación, creo que yo me excedí un poco, y no olvidar alimentar a tiempo ya que beben mucho. Realmente contento con esta experiencia Muchas gracias por pasar y felices cultivos!!

Tried lst think I fucked up please help trying to get plant to stretch

Vegetation Phase - Week 5 Week 5 marks a bittersweet milestone. RIP to the Marienkäfer homie — gone but never forgotten. Their watchful eyes and pest-snacking legacy will forever live on in the tent. Meanwhile, the Donutz gang is thriving and about to hit the next big step in their journey: the 7-liter pot upgrade. Updates & Changes: RIP Marienkäfer: Nature giveth and taketh away. The tent feels a little emptier without our little guardian, but pest pressure remains nonexistent thanks to their diligent work. Transplant Time: The plants are moving up to 7L pots this week, giving their roots the room they need to expand and thrive before flipping to bloom. This upgrade will ensure a strong foundation for the explosive growth coming soon. Watering: Still sticking with reverse osmosis (RO) water for maximum control and cleanliness. Plan for Flower Transition: After the transplant, the plants will chill for the rest of the week in their new homes to recover and adjust. Week 6 will cover the flip to flower. With the clean lollipopped structure and solid root development, these plants are set to focus all their energy on producing hefty main colas in the Sea of Green setup. Observations: The Donutz are stacking beautifully, with healthy green leaves and strong stems. The SOG canopy is filling out, and each plant is shaping up to deliver a uniform, productive grow. Next Steps: Monitor the plants closely after transplanting to ensure they adjust smoothly to the 7L pots. Maintain stable environmental conditions to avoid stress during the final veg phase. Prepare for the flip to flower in Week 6 — the real show is about to begin!

She suffered with under watering practices and heat stress no doubt but seems to be stabilising with no further deterioration. Double Kush Cake Feminized is come to the end of her growth spurt reaching 60cm her gaps aren’t filled and won’t likely be filled with due to the stress she was under! From now until the end the lady will be fed sparingly every five days with 1500ml of nutrients and npk

Very happy this week as my friend gave me a female Mac x Purple Punch he was using as a mother plant. That is in an 18 litre soil pot. The 3 Rado Z are now in 15 l autopots fitted with airdomes. A soil mix was made with dutch vega bio soil , Bio grow and silicium flash. The MacPunch I have stripped the lowers and trained it using easy bend clips.