Day 114. Gorilla Max is totally ready, maybe she would bulk up, but last white pistil went brown at least a week ago. Time to trim. Bud not solid, but kinda hard, bottom all airy and small, but she smells and sticky like hell, so fruity ... Had to chop her, because got tiered of seeds everywhere, so cleaned my draws and fridge, from 45 found bag seeds started new diary, had to make space for them. Want to try out 12/12 from start and even if i would leave them under natural sun , they would stretch too much in first stage of grow and would repeat that grow patern indoors. Scissors hash was nice, super soft, hard to roll, all stays on hands. On this plant found two seeds, maybe some hidden in colas, but its not even close to bad that i was imagining at the beginning. My watering damage is obvious, Gorilla Max and Bunny were best plants, now - they are smallest... Last weeks very important, till week 5 of flower and that incident they were leaders, overdrying don't work . Hope i will learn this lesson. Day 120. Last 3 girls are down. Gorilla Max - 88 g. Of dry bud. Think she and Bunny will be smallest of them all. Buds are airy, but very sticky and all calaxies went pink. So in spliff it looks really funky. Needed to lolitop them higher, maybe overall look and cola structure would be better then. Lanky Gorilla is a champ, no doubt. Think this girl should beat 4 ounces easy. Day 126. So total of all 4 plants is 401. A bit less than was hoping, but target achieved. Update will follow.

Day 1 week 12. Has it been that long? Wow. Nothing dramatic yet. The twins seem to like the new feeding recipe. Drinking a bit more now that they are flowering and winter is here so the air is drier. Day 4 week 12. Starting to see frost. and even some amber on the top of the bud sites. hope it is natural and not premature aging.

..somit geht die 4 Blütewoche zuende. Langsam wirds frostig ?

Sin complicaciones.

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (LIME GREEN CHLOROSIS) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.

Last week of veg

Les sites de bourgeons commence a ce développer, défoliation cette semaine pour optimiser les branches. La fréquence nutrionnel 1fois sur 2. Le ph est contrôler, niveau EC au drainage contrôler. Merci a toute l'équipe @Dutch Passion bravo. 👏🏽☝️

So 7 days after the start of germination all seeds have germinated and broken through the soil, only one of the seeds haven’t yet unfolded her leaves and that might have been because i helped her get the seed off to early but overall it is looking good and week one of veeg is about to begin.

nice harvest , very resinious , gluing my fingers. i ll update when it will be dry. i hope something like 100g on this plant but i will be happy if i have 80g. i dried a bud in oven to try the effect , it's mainly indica effect, goos to watch a movie or doing relax activity, to drawing, or making music.

Day 85. Girls are packing up , still flowering like nothing. I have other diary with moved Kushes running and explained there a bit more why i think i have that leaves discoloration. Still lost what happened, but measures were taken, girls got EcoThrive Microbe Tea, Epsom salt , now its only to finish them. 3 plants from 8 got some deficiency and the only variation was new brand of molasses for me. Let's see will it stop the spread or i have to find real problem .... I was always using Cal-Mag not by Biobizz, this grow i tried to use theirs, but simple epsom salt works better and faster... Drifting away from organics a bit ;))) Skunks looks like a faster strain, Cheeses just packs flowers, no brown pistils at all. In my mini hopes they are 5 weeks away from harvest. Happy Growing and Merry Christmas !!!

Everything looking a lot better

Heute wurde der Autopot abgedreht. An Ostern gibt's dann Geschenke 😉 Holla die Waldfee. So harte Buds hatte ich noch nicht im Zelt! Delicius 🤤 Die neue Lampe gefällt mir.

At a total of 79 days this Sangria is a beautiful one! A cross between The GOGO and Ogreberry she is super delightful, Dense resiny heavy covers trichome buds , with the smell of Gassy rotten fruit funk on the nose! She a stanky one ! Other then that she was a pretty easy grow , y’all don’t sleep on it an better get your hands on it , at @Twenty20Mendocino they got you covered

💩Holy Crap We Are Back At It And Loving It💩 Growing in a 11 ounce pot 👈 👀 👀 👈 Growmies we are at DAY 56 and she's just killing💀it👌 TALK ABOUT FROSTY 👈 Amazing results thus far for the Tiny Pot 😀 👉Added straws within the Tiny pot to help watering in week 4 👈 So I'm starting to see she needs watering every single day and now need nutrients 🙃 Lights being readjusted and chart updated .........👍rain water to be used entire growth👈 WATERING SINGLE TO TWICE DAILY 😋 👉I used NutriNPK for nutrients for my grows and welcome anyone to give them a try .👈 👉 www.nutrinpk.com 👈 NutriNPK Cal MAG 14-0-14 NutriNPK Grow 28-14-14 NutriNPK Bloom 8-20-30 NutriNPK Bloom Booster 0-52-34 I GOT MULTIPLE DIARIES ON THE GO 😱 please check them out 😎 👉THANKS FOR TAKING THE TIME TO GO OVER MY DIARIES 👈

The babies 😍 have a beautiful look and colors, they seem quite filled with resin. Now the drying phase of 15 days begins after which I will complete the trimming and weigh it. By eye I can say that it will be about 100gr per plant

Very explosive growth this week! She is easily the tallest girl in the tent! No wonder she is called Jumping! Things progressing very well.

Defo hit a stretch and are well into flower now .. looking good and healthy,.. roll on next week 👌

cloudy week grew 7.cm and looks healthy but slow ... the temperature has dropped ... I think she will be even slower outdoor ... .

I got the purple pheno at last !! So glad actually... that it is actually changing colour and with so many more weeks left for it to continuously transition. Totally what I’m looking for in future breeding projects. Black amnesia haze fast: for its rooting and overall vigorous performance. Creating good yields each run And this one I’m still waiting to see how she goes! Way more grows in the future!