day 8 - i think i might have a small issue. i think the ph level of the coco is a bit high, my runoff water is about 6.8, i really want to get the run off down to under 6.5 ... the coco might be a little bit too salty because its new the kush plant is yellowing, which im assuming is from overwatering. im going to not water my plants today and see how they take it. i watered heavily last night to try and flush out some more of the salts / lower the ph of the coco. i ordered a new light last night, the HLG320 4000k. i am tired of having to adjust the hlg65 and hlg135 seperately, so i figured might as well go with a light thats the full length of my tent. eventually, ill throw in an hlg320 3000k, have both turned way down, so during flower the coverage is very even. also, im debating getting my light turned down on the seedlings right now. now its around 30-35k lux, but i might have it turned down into 25k lux range for the day. i day 10 - a little bit of tacoing on charlottes #1 and cheese #2, turned down the light intensity by about 10%

Giorno 56 Zombie Kush #1 è pronta. 20% di tricomi ambrati e quindi si taglia. L'avrei lasciata volentieri un'altra settimana ma ho i barattoli vuoti 😀 Zombie Kush #2 ha ancora tutti i tricomi latte e rimarrà almeno un'altra settimana. Cmq bellissime cime. Tutte di uguale consistenza e colori stupendi Le due Rainbow sono praticamente identiche e tra qualche giorno verranno tagliate visto che hanno già un buon 10% di tricomi ambrat La Milk Monkey ne ha ancora per due settimane e mi sta colpendo particolarmente. Penso sia la pianta più collosa mai coltivata. A settimana prossima e grazie dei like e dei commenti ❤️

Week 9 day 1 video pheno. Gonna chop this week Week 9 day 7 well chop this weekend so on week 10 Day 1-2. Just waiting on a light to reveg one. Everything is magic

day 16 in flower. very little stretching on this Pheno. and she's started showing her purple already. I'm super excited and hope I get my taste and high on point. thanks for sticking with me. 🙏🏿💯

8/30 Rained yesterday and pretty much all night. Plants looked happy this morning. I'm thinking about feeding today or tomorrow. The late flowering special kush doesn't have much longer and it will be done. I'm going to need to do another treatment for the thrips. Nights are so cold that I think they aren't a major problem. No signs of septoria either. No signs of ANY molds or mildew. Even where I took the pillar out but I removed a lot. I am noticing (especially on the pink kush and the special kush in the back) the wierd "top hat" lower buds like I previously did. I don't think it's the chipmunk. It's like they eat fresh buds and leave the top there. I think it might be earwigs but I haven't seen ANY. Birds have been keeping my garden pretty clean. Almost everytime I walk down there it's like a flock of birds takes off lol. I was watching the little finches all perched on my cage. I've seen them dart in and grab a snack. It's pretty cool how it works. Symbiotic relationship. I feed the birds amd they help keep my garden pest free. EDIT: Went to check the girls around 430. It seems like everytime I see the flowers they've swelled in size. Especially the natural mk ultra. Everything is really coming together. Pink kush is behind but it's starting. I'm getting lots of fall colors but I'm losing lots of leaves too. I wouldn't have defoliated as good as I have if I hadn't had problems. I thought the plants were done getting taller or stretching but after this rain they ALL seem taller. The buds are swelling as well. I'm on the home stretch with the early special kush. Few more weeks. Everything looked amazing tonight. I wanted to take some pictures but I left my phone. Smell is greatly increasing. 8/31 I don't get how we got NO rain the ENTIRE summer but it's rained the past few nights? Maines bipolar weather I guess. I see the same shit as prior years. Something crawls up and eats small shoots and developing buds but leaves larger ones alone. I thought it was earwigs before. I'm not sure now. I'll have to go look at night and figure out what's eating shit. It's just small secondary shoots mostly. Stuff I should've pruned but I leave on so the won't eat my fucking colas. I got some work to do but I'm getting closer. 9/1 Well we made it to September. Pink kush is finally STARTING to form little buds. After some research (and watching those fucking locusts try to fly into my cage but can't because they are to fucking big. I was lucky I found that enormous one on the stalk of the furthest special kush right where the tip cola starts. Luckily I was able to get him. Didn't have to take pics or a video or even check if they need water. It rained a little last night and the buds were a little wet. I'm planning to feed when I get back. If they need water I'll give it to them. My early special kush is getting real close. I want to harvest at peak timing but sometimes exigent circumstances screw that up. However i think I'll be able to let this girl finish properly. EDIT: Went back over to check weights and everything was super heavy. Plants are all turning fall colors. I checked the trichs on my special kush, mk ultra and 10th planet. Everything looked great in the sun! Everything is swelling and getting frostier everyday. I'm hoping that the pink kushs will finish. My early special kush LOOKS like it should be done but it's not. Looking at the trichs and stigmas it needs more time. I've found on several plants that between the; giant caterpillars, earwigs and pillars that they can take a whole branch and pretty much decimate it in a night. I chased one of those giant locusts around my Cafe and it finally got out. Bastard was almost the size of a small bird! I should feed tonight and possibly do a treatment for pests. 9/2 Had to shake to plants off again. Bags are still heavy. I'll probably feed tonight. I'm noticing tons of damage on lower and interior branches. The grasshoppers or locusts or whatever they are can strip a whole branch! They can even bite through little stems. I'll never grow outside without floating row covers again. The special kush is getting really close. Sometime this week I'll look for a mature tester bud. Both MK ULTRAS are exploding in flower and getting frostier daily. 9/3 We are going to be getting some rain. I noticed the grasshoppers or whatever really like the special kush. After seeing her in the sun I made the executive decision (after one more peak at the trics) and decided to chop her. I feel like that was a good decision. Even more so as I got going. I bet if I let it ride I would've ended up with half as much. The fan leaves had all dropped. I wish I would've kept a clone of this pheno. Smells amazing! I did a quick wet trim with branches and cut little buds (which there weren't many) went in a paper bag the rest was hung to dry. Minus the top cola and a bud or two I brought home to look at and manicure. The roots were really dry. The others seen heavy but I haven't watered in a while. I'm planning on watering and feeding tomorrow. Things are really exploding and conibgvti life! I feel so much better having that plant inside. 9/4 I was definitely right to take that plant. It just SEEMED to early. Went over today and found more damage from those grasshoppers or locusts, whatever the fuck they are. I used to just hear that noise at night but now it's morning noon and night. Some can't hear it but it's this high pitched buzzing (them rubbing their legs together) which wad heard occasionally with crickets at night. This hear it's a CONSTANT sound. Considering I'm in late flower I'm not sure what to do. I don't think there's much u can do. At least it seems like they prefer the special kush and target that. So at least that huge mk ultra and the natural one and the 10th planet's should be fine. Pink kush is finally flowering. Whether they'll have any nodes left by the time they finish is an unknown. WATERED TODAY. EVERYTHING GOT A GALLON BUT THE 10S. EVERYBODY GOT A QUART OF FOOD. IM CONSIDERING UPING KOOL BLOOM. Didn't take pictures or do a video. I will later. I'll review the special kush I took when I take the others. I'll be back over later and I'll update. No rain as of 2pm. I watered because I took the rootball apart of the special kush I harvested and it was dry as a popcorn fart! I lifted a couple and they were light bit I didn't lift everything. The water sat on the top for quite before it would drain. At least they had a good dry cycle. That plant I took looks and smells amazing. I can't wait to try it. 9/5 Thunder showers today. Started raining early while I was checking the girls out. I had coffee and waited until it stopped and shook them all off. EVERYTHING is exploding in growth. Pink Kush is still quite a bit behind but everything else has swollen significantly. It's going to rain tomorrow too which I'm sure the plants will like. I SHOULD go stake individual branches or at the very least add some more supports. Still losing some stuff to critters but whatever. It's nature's claim. That plant I took looks awesome. My intuition was spot on. Can't wait to try it. EDIT: WENT BACK OVER AND SHOOK OFF BRANCHES AND ADDED SOME SUPPORTS. I DEFOLIATED A SHIT TON OF LEAVES. FLOWERS ARE GETTING BIGGER EVERY DAY. I FOUND SOMETHINGVTHAT LOOKED LIKE "STEM ROT" ON THE BRANCH OF SPECIAL KUSH THAT GOT STRIPPED. I LOOKED UP WHAT TO DO BUT FORGOT TO MENTION IT WAS SPRINKLING AND I CUT THE BRANCH OFF. THIS WAS (OR WOULD HAVE BEEN) A LARGE BRANCH AND IT WAS ALL GONE. IM HIPING IT DOESNT EFFECT THE PLANT. ITS ANOTHER SPECIAL KUSH AND ITS THE ONE WITH PINK CALYXS. I'M TRIMM8NG THE SPECIAL KUSH I TOOK DOWN. I GOT TO TRY IT TODAY. IT TURNED OUT AMAZING! COVERED IN TRICHS, SMELLS GREAT BUT TASTES BETTER! GIVES ME THAT FAMILIAR INDICA HIGH WE ALL KNOW AND LOVE. I DONT KNOW WHAT IT IS BUT IT SEEMS THAT CANNABIS GROWN WITH YOUR OWN HANDS FOR YOUR OWN NEEFS SEEMS TO WORK BETTER FOR ME THAN DISPENSARY PIT I KNOW IS BETTER. IM SITTING HERE WITH A SMILE ON MY FACE. SINKONG INTO THE COUCH. I CAN TELL THERES OTHER CANNIBINOIDS AT WORK TOO. I LOVE THE ENTOURAGE EFFECT!

no he podido actulizar la semana pasada ya he hecho muhas cosas pero estoy de vuelta. comenzando la semana solo hechaba agua nada mas ya q tenia pensasdo cosechar la mitad de la planta el viernes/sabado ya q estaba haciedno mucha humedad por el frio y estaba apareciendo botritis y hongo rn algunos cogollos.

Hi growers friends. The Zkittlez came into the darkroom yesterday for 48 hours and will be harvested tomorrow. the Blue gelato 41, Orange Sherbert and Her Majesty F1 need 1 week to harvest.

Buenas gente estamos aquí con la 5 Semana de El Patrón estamos echando el producto grotek una vez por semana está dando un buen resultado va creciendo fuerte y con un aspecto muy bonito y emos bajado el foco a 30 cm en general esta semana a ido muy bien an dado un buen crecimiento.👌👌👌😉 Vamos a ver en el concurso de RQS!!!!🔥🔥

Day 85. Few days ago I trimmed most leafy Northern Lights and she loved it ! So tomorrow two most mature Alaskan Diesels go to chop room. All other plants will get defoliation too, will have space to reach all plants. Third Alaskan Diesel is catching, but he is a week behind. Green Cracks will fall in 2-3 weeks, Northern needs at least 4 .... Will move my Bruce Banner's to main tent. I still feed Northern , but all other plants are on pure water. Day 86. Rearranged all girls, trimmed them heavy, gave new positions. One Northern lights is still in dark part of tent, I think I need 7-10 days til last Alaskan Diesel will mature and it will be only 8 girls left. Brought other diaries girls to main tent and added Mars Hydro SP150 as supplemental side lightning ... They miss height a lot compaired to older plants, so I hope it will help a bit . Never used lights that way before ... Happy Growing !!!

🎄🎄🎄 Good day, bud lowers 👨‍❤️‍👨 👨‍❤️‍👨 👨‍❤️‍👨 👨‍❤️‍👨 🌲 Continues to stretch the girls in ScrOG. Within a week, the plant bummed to growth, look what happened! 🌲 ✂️ I do defoliation once a week, because it is difficult to fit such a plant under the net. ✂️ 💚 The buds are gaining mass really fast. I was surprised comparing Monday and Friday pictures. ⭐⭐⭐⭐⭐⭐⭐⭐⭐⭐ ✅ Harvest is coming. Therefore, the last watering with fertilizers will be next week, then only clean water and delicious things like sugar. ✅✅ 🙈 Are you ready to guess the result? In this report, the variety is revealed in full force. 🙈 🌲 Almost all trichomes are cloudy, there are practically no amber ones. New stigmas are still springing up. 🌲 ☢️ The plant very actively began to take nutrients from the leaves - the yellowness is noticeable. ☢️ 🌝 Please check this week pictures and small video I made for you .,🐛

Green Cure CBD Auto germinated and has sprouted easily. Every seed I started all germinated. So she is getting acclimated, and should start growing very soon. Everything is looking good. I did provide a light feed to help with ph stability in coco. She is off to a good start. Thank you Medic Grow, and Zamnesia Seeds. 🤜🏻🤛🏻🌱🌱🌱 Thank you grow diaries community for the 👇likes👇, follows, comments, and subscriptions on my YouTube channel👇. ❄️🌱🍻 Happy Growing 🌱🌱🌱 https://youtube.com/channel/UCAhN7yRzWLpcaRHhMIQ7X4g

I turned the autopots on Monday and the plants are really thriving. They are growing super fast and the soil mix seems to be performing well. They have just had pH 6.4 water from the water reservoir. I have tied and bent them twice since Monday but they bouce back so fast. I have also turned in th airdomes , buried at the bottom of the pots this week, so let's see what they can do. Plenty of defoliation to expose lower branches , it always amazes me how quick the removed leaves are replaced by little tops. Day two flower more pictures added. Loads of defoliating this week and tied them all down again yesterday.

Another amazing week with these beauties I am still feeding the same monkey nutrients couple seem to be burning through the feed quicker so I have upped the doses back to the full doses as I had dropped them slightly previously but they are so sticky 🙏🙏 so I am trying to ensure the air circulation stays constant and the humidity stays at around 42 with the increased temperatures we have had here in the UK I have turned my extraction fan up to near jet engine status 😂😂 thankfully its not really any louder than a fan so it's not been to bad and the carbon filter is doing a really good job at keeping the danky smell in the tent personally I love the smell not sure the neighbours would so much 🤣😳 in inlet fan and circulation fans are working hard but managing to keep temps from going above 29 there was one day I set Zues pro at 75% just to help temps drop The buds are really starting to get heavy now and there is literally no room left for anymore the highest tips are probably 4f 8 the tent is only 6ft 6 so the Zues pro 600 is as high as it can go now so thank goodness the home stretch is not that far away now. So I am really talking to them lots giving them lots of love and attention and preying these beauties continue to do there thing and grow there buds nice and fat with no issues 🙏👌 Thank you for reading stay blessed 💚

Week 11 Flower Report for the Anesia Run 🌸🌿 Hey Grow Diaries fam! 🌱✨ Welcome back to another exciting update on our Anesia run. We’re deep into Week 11 of flower, and let me tell you, the garden is absolutely thriving! The girls are showcasing their full potential, and the canopy is a vibrant sea of frosty buds. Grab a cup of your favorite brew, sit back, and let’s dive into this week’s report filled with fun facts, details, and a sprinkle of positivity! 🌟🌸 Nutrient Update 🌿🔬 This week, we’ve continued with our nutrient regimen, making a few key adjustments to ensure the plants are getting everything they need to produce those sticky, resinous buds. Our mix includes: • Regulator: Keeping the plants’ stress levels in check and ensuring optimal growth. • CalMag: Providing essential calcium and magnesium for strong cell walls and overall plant health. • P-Boost: Enhancing flower development and increasing bud size. • K: Introducing potassium to boost bud density and improve overall flower quality. • Top Booster: Supporting the final push in flower production, ensuring those buds are as big and healthy as possible. Our TDS has been adjusted to 500, with a pH of 6.0, keeping the environment perfect for nutrient uptake. 🌿💧 Environmental Control 🌞🌡️ Temperatures have been steady at 21°C, creating the ideal environment for our girls. Consistency is key in these final stages, as we want to avoid any stress that could affect bud development. 🌡️ Fun Facts & Details 🌸✨ 1. Trichome Wonderland: The trichomes on these ladies are sparkling like diamonds. Did you know that trichomes are the plant’s way of protecting themselves from UV rays and pests? They also contain the highest concentration of cannabinoids, making them the heart of the plant’s potency. 2. Pistil Party: The pistils are starting to turn a beautiful amber, signaling that harvest time is approaching. Fun fact: pistils are the female part of the flower and are responsible for catching pollen to produce seeds. In our case, no seeds, just beautiful, potent buds! 3. Aroma Explosion: The terpene profile is off the charts. Walking into the grow room is like stepping into a fragrant garden. Each strain has its unique scent, from the sweet and fruity notes to the pungent and earthy undertones. Did you know terpenes not only provide aroma but also enhance the effects of cannabinoids through the entourage effect? 4. Leaf Yellowing: Some of the lower leaves are beginning to yellow and fall off. This is perfectly normal as the plant focuses its energy on bud production. It’s a sign that our girls are nearing the end of their life cycle, putting all their effort into flowering. 5. Bud Density: The buds are getting denser by the day. Proper potassium levels have been crucial in achieving this. Dense buds mean more weight and better quality, making all our efforts worthwhile. Special Shoutouts 🌟❤️ A massive thank you to our amazing community and all the support we get on Grow Diaries! Your comments, tips, and encouragement mean the world to us. 🌍✨ Anesia Seeds, thank you for these incredible genetics. Each plant is a masterpiece in its own right. 🌱❤️ To our sponsors Aptus Holland and Future of Grow LED, your products have been pivotal in creating this green oasis. And let’s not forget the TrolMaster for keeping our environment in check. For everyone following along on YouTube, don’t miss out on our series Welcome to the Green Oasis. This week’s episode dives deep into the latest updates, techniques, and everything you need to know about growing these beautiful plants. 🎥🌿 Invitation 🌟 Don’t forget to check out our YouTube channel for more detailed insights and exclusive content. Let’s grow together and spread the positivity! 🌿🌞 Conclusion 🌸✨ Week 11 has been a journey of growth, learning, and joy. The girls are almost ready to reward us for our care and patience. Stay tuned for the final stages as we approach harvest, and as always, keep growing and glowing! 🌟🌱 Thank you all for being part of this journey. Until next time, happy growing! 🌿❤️ #GrowDiaries #AnesiaSeeds #AptusHolland #FutureOfGrowLED #TrolMaster #CannabisCommunity #HomeGrow #CannabisCultivation #PositiveVibes #KeepGrowing #WelcomeToTheGreenOasis #Episode11 Apricot Oreoz = AO Zoomiez = Z Future#1 = F https://anesiaseeds.com/ Plant nutrition - Aptus Holland - https://aptus-holland.com LED Photons - Future of Grow - https://www.thefuturofgrow.com/ Controls - Trol Master - https://www.trolmaster.eu/ Watering - Autopot - https://autopot.co.uk/ Tent - Mars Hydro - https://marshydro.eu/ Love and attention - Me, myself and i As always thank you all for stopping by, for the love and for it all , this journey of mine wold just not be the same without you guys, the love and support is very much appreciated and i fell honored and blessed with you all in my life, With true love comes happiness. Always believe in your self and always do things expecting nothing and with an open heart , be a giver and the universe will give back to you in ways you could not even imagine so More info, the ocasional give-away and exclusive updates from all my adventures can be found - links in the profile description Friendly reminder all you see here is pure research and for educational purposes only Growers Love To you All

Dinamed Plus from Dinafem Seeds (1) and Blueberry Headband (1)from Humboldt Seeds. The OGKZ Fast Flower Seeds haven't arrived from Humboldt yet, so I've replaced it with a Blueberry Headband clone. Everyone's doing well! https://www.dinafem.org/en/dinamed-cbd-plus/ https://www.humboldtseeds.net/en/blueberry-headband/ Lighting https://www.horticulturelightinggroup.ca/products/260w-qb-v2-led-kit DWC Nutients https://generalhydroponics.com/floraseries FloraGro FloraBloom FloraMicro Diamond Nectar https://generalhydroponics.com/diamond-nectar Cal Mag https://generalhydroponics.com/calimagic SuperThrive https://superthrive.com/

- Día 1 de la segunda semana de flora con riego foliar de calmag - Videos tomados día 2 de la segunda semana

Whats up yall! We're here at the end of week 6 in flower! Some rearranging and formula shifts have occured as we head into the final push of flower. 7 plants are being fed 3.6/1.5/1.5/0 part B while the others continue the Jacks full strength plus 1.2 MKP. I have also began giving recharge and molasses to the plants every 10 days instead of 21 - I've read/watched/listened to some people and it sounds like more microbes can be good and at worst, not harmful. At this time, GDP seems to be first up with Platinum OG or Purest Indica on deck. Permanent Marker and Bag Seed [to be named] should be next and finally Pineapple Express #3. They're all at least 2 weeks away from harvest/flush and I will be monitoring trichome development closely to determine a target harvest day. The remaining 5 need closer to 4+ weeks before they're ready to be chopped. Things are looking good and are moving along very steadily! I cannot wait for the end of the month! Until then, I will see you guys next week. Feedback, comments, and questions always welcome! Next time I see you it'll be 420 😎

Dried and now in Curing

7/28 1220AM MONDAY.... Bubbles are looking ok, I need to trim them back to 4 colas, I am really stressing them with small space limitations, and temps at 80 to 81 7/29 Tuesday 5:45 AM💦💦 6:15 AM water with methylene blue two pills of nitric boost amino acid, five drops of Quick Sorb and I added bud factor X today and a quarter mL per liter of rhino silica💦💦💦. 1030 AM I don’t know what I’m gonna do. It turns out the bubbles plants are terrible. The stems are all very weak and they’re growing bushy at the top. I didn’t get them separated so all of the side branches grew up really long it’s completely useless. It is absolute this plant. It will be thrown in the garbage. Can you fucking believe I have to throw another entire plant in the fucking garbage😡😡😡😡. TREVOR plant cut down to 2 colas only, very top heavy not liking my grow space, had 2 more than I wanted so it's all fucked.... will remove shitty small colas and get maybe 2 good colas per plant, not expecting much. 1219pm....HOLY SHIT I FUCKED THESE.....TOOK OFF SOME COLAS.....NOT ENOUGH SPACE AND I PUSHED THEM TOO FAR.....I CAN FIX IT 7/30 1215am found a GREAT science link::: ""https://www.frontiersin.org/research-topics/8125/cannabis-genomics-breeding-and-production/magazine"". 345am PROLINE WILL BE HERE TODAY.ALL RESEARCH INDICATES PROLINE WILL PROTECT FROM HEAT STRESS!!!! 11:30 AM they are good again they are recovering from heavy defoliation half of the plant was taken off of one of them. I pushed it a little far but today they are recovering from heat and lack of air and lack of space the leaves are reaching up and they are not folding under heat stress. They look good today. Fast recovery heavy amino yesterday.👍👍👍👍🚨🚨🚨🚨 🚨🚨🚨🚨🚨🚨🚨🚨 GOT THE AMINOS AND HERE IS THE GROK RESULT OF MY MIX.......🚨🚨🚨🚨🚨🚨🚨🚨 The effects of applying essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine to cannabis plants can be significant, as these compounds act as biostimulants, influencing growth, stress resilience, and secondary metabolite production. The available research, primarily from studies on amino acids in plants, including cannabis, provides insights into their roles, though specific data on this exact combination is limited. Below, I summarize the effects of each compound on cannabis plants, drawing from relevant studies, and infer potential synergistic effects based on their known roles in plant physiology. Note that TMG (betaine) has less direct research in cannabis, so some effects are extrapolated from other plants. ### 1. Essential Amino Acids (EAAs) EAAs (e.g., leucine, lysine, methionine, etc.) are critical building blocks for proteins and play roles in plant metabolism and stress response. In cannabis: - **Growth and Development**: EAAs enhance protein synthesis, supporting shoot and root growth. Foliar or soil application of EAAs (e.g., 150–300 mg/L) increases leaf chlorophyll, shoot biomass, and root development in various plants, likely applicable to cannabis.[](https://www.nature.com/articles/s41598-022-21273-6) - **Nutrient Uptake**: EAAs like methionine and lysine improve nutrient absorption (e.g., nitrogen, iron) by acting as chelators, potentially enhancing cannabis nutrient efficiency in hydroponic or soil systems.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) - **Stress Mitigation**: EAAs, particularly methionine, support antioxidant production (e.g., glutathione), reducing oxidative stress from drought, salinity, or high light, common in cannabis cultivation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2020.00928/full)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cannabinoid/Terpene Production**: Limited evidence suggests EAAs may influence secondary metabolism. For example, methionine could enhance sulfur-containing compounds, potentially affecting terpene profiles in cannabis.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) **Application Notes**: EAAs are often applied via foliar spray (150–300 mg/L) or soil drench. Overuse may lead to nitrogen imbalance, so moderation is key.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### 2. Proline Proline is a non-essential amino acid that accumulates in plants under stress and serves multiple functions: - **Stress Tolerance**: Proline acts as an osmolyte, stabilizing cell membranes and scavenging reactive oxygen species (ROS) during drought, salinity, or temperature stress. In cannabis, exogenous proline (e.g., 50–100 mM) may enhance tolerance to environmental stressors, improving resilience in outdoor or controlled environments.[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW)[](https://pmc.ncbi.nlm.nih.gov/articles/PMC3548871/) - **Limited Growth Effect**: Proline alone is a poor nitrogen source for growth. Studies on Arabidopsis show it has a neutral or inhibitory effect on growth when used as the sole nitrogen source, suggesting it’s better for stress response than biomass accumulation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Cannabis-Specific**: Proline helps cannabis maintain water balance, potentially improving resistance to extreme temperatures, which is critical for outdoor grows.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) **Application Notes**: Foliar or root application (50–100 mg/L) is effective for stress mitigation. High doses may inhibit growth if not balanced with other nutrients.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full)[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW) ### 3. Trimethylglycine (TMG, Betaine) TMG is less studied in cannabis but known as an osmoprotectant and methyl donor in other plants: - **Stress Protection**: TMG stabilizes proteins and membranes under salinity, drought, or temperature stress, similar to proline. It may protect cannabis plants in harsh conditions, maintaining photosynthetic efficiency.[](https://www.mdpi.com/2311-7524/9/7/807) - **Methylation Support**: TMG donates methyl groups, potentially enhancing secondary metabolite synthesis (e.g., cannabinoids, terpenes), though direct evidence in cannabis is lacking. Its role in methylation could support metabolic pathways linked to EAAs and glutamine. - **Growth Effects**: Limited data suggest TMG may improve chlorophyll content and photosynthesis, indirectly supporting biomass in cannabis. **Application Notes**: TMG is typically applied at 0.5–2 g/L via foliar spray. Its effects in cannabis are inferred from other crops, so careful experimentation is needed. ### 4. Glutamine Glutamine is a conditionally essential amino acid central to nitrogen metabolism: - **Growth Promotion**: Glutamine (10–1000 mg/L) significantly enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and hibiscus, likely benefiting cannabis vegetative growth. In cannabis, it may support lush foliage and robust rooting in hydroponics.[](https://www.scielo.br/j/oh/a/GZxMgqXb9wm7bsCTv4WwPdc/?lang=en)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html?lang=en) - **Nitrogen Assimilation**: As a primary nitrogen acceptor, glutamine improves nitrogen use efficiency via the glutamine synthetase/glutamate synthase cycle, enhancing nutrient uptake in cannabis.[](https://academic.oup.com/jxb/article/58/9/2339/544408)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Stress Response**: Glutamine supports proline synthesis, boosting stress tolerance. It also contributes to chlorophyll and protein synthesis, improving photosynthesis and yield potential in cannabis.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Secondary Metabolism**: Glutamine is a precursor to glutamate, which influences GABA, arginine, and proline synthesis, potentially affecting cannabinoid or terpene production indirectly.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) **Application Notes**: Foliar sprays (250–1000 mg/L) or root application twice weekly are effective. Glutamine at 250 mg/L combined with other amino acids (e.g., glycine) shows synergistic growth effects.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) ### Synergistic Effects on Cannabis - **Growth and Yield**: The combination of EAAs, glutamine, and proline may enhance vegetative growth and biomass. Glutamine and EAAs drive protein synthesis and nitrogen uptake, while proline supports tissue integrity under stress. TMG likely amplifies these effects by improving cellular hydration and methylation, potentially increasing leaf area and bud quality.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.nature.com/articles/s41598-022-21273-6) - **Stress Resilience**: Proline and TMG act as osmoprotectants, while EAAs and glutamine bolster antioxidant systems, collectively improving cannabis resilience to drought, salinity, or temperature extremes common in cultivation.[](https://www.mdpi.com/2311-7524/9/7/807) - **Secondary Metabolites**: Glutamine and TMG may enhance cannabinoid and terpene production by supporting metabolic pathways (e.g., methylation, nitrogen metabolism). EAAs like methionine could influence sulfur-based terpenes. However, direct evidence for cannabinoid enhancement is sparse.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Nutrient Efficiency**: Glutamine and EAAs improve nitrogen and micronutrient uptake, while TMG may enhance nutrient transport, optimizing cannabis growth in nutrient-limited systems like hydroponics.[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) ### Potential Risks and Considerations - **Inhibition at High Doses**: High concentrations of proline or certain EAAs (e.g., lysine, valine) can inhibit growth if used as sole nitrogen sources. Balanced mixtures mitigate this, as glutamine can antagonize inhibitory effects of proline or other amino acids.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Nitrogen Overload**: Excessive EAAs or glutamine may lead to nitrogen toxicity, causing leaf burn or stunted growth in cannabis. Monitor plant response and maintain balanced nutrient solutions.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **TMG Metabolism**: TMG may increase trimethylamine production in the soil microbiome, potentially affecting cannabis root health if not managed. Healthy soil microflora can mitigate this.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **Application Timing**: Foliar sprays (150–500 mg/L for EAAs, glutamine, proline; 0.5–2 g/L for TMG) during vegetative growth or early flowering maximize benefits. Avoid overuse during late flowering to prevent nutrient imbalances affecting bud quality. ### Cannabis-Specific Insights - **Hydroponics and Soil**: In hydroponic systems, EAAs and glutamine ensure efficient nitrogen delivery, while proline and TMG support stress resistance in fluctuating pH or nutrient conditions. In soil, maintaining microflora is crucial, as it naturally supplies amino acids; supplementation should be moderate.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cultivar Variability**: Cannabis cultivars differ in nutrient demands and stress responses. Sativa-dominant strains may benefit more from proline and TMG for stress tolerance, while indica strains may respond better to EAAs and glutamine for dense bud growth. Testing is needed. - **Secondary Metabolites**: While amino acids influence secondary metabolism, direct effects on THC or CBD levels are unconfirmed. Glutamine and TMG may indirectly enhance terpene diversity via improved photosynthesis and methylation.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) ### Practical Recommendations "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨 - **Dosing**: Apply EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) via foliar spray or root drench twice weekly during vegetative growth and early flowering. - **Combination**: Use a balanced mix (e.g., 250 mg/L EAAs + 250 mg/L glutamine + 50 mg/L proline + 500 mg/L TMG) to avoid inhibition. Test on a small batch first.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Monitoring**: Check for signs of nitrogen toxicity (dark green leaves, clawing) or stress relief (improved vigor, greener leaves). Adjust doses based on plant response. "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to enhance uptake.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient lockout. In soil, ensure healthy microbial activity to complement amino acid supplementation.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### Evidence Gaps - **Specific Combination**: No studies directly address EAAs, proline, TMG, and glutamine together in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid Impact**: Limited data on how these compounds affect THC/CBD levels. More research is needed on secondary metabolite pathways.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **TMG in Cannabis**: TMG’s effects are extrapolated from other crops. Cannabis-specific trials are needed to confirm benefits. ### Conclusion Applying EAAs, proline, TMG, and glutamine to cannabis plants can enhance growth, stress tolerance, and nutrient uptake, with potential benefits for yield and secondary metabolite production. EAAs and glutamine drive biomass and nitrogen efficiency, proline and TMG bolster stress resilience, and their combination may optimize cannabis health in challenging environments. However, careful dosing and monitoring are crucial to avoid inhibition or toxicity. For best results, test small-scale applications and consult with a horticulturist familiar with cannabis cultivation. If you want more details on application methods, specific cultivars, or further searches for cannabis-related studies, let me know!""🚨🚨🚨🚨 🚨🚨 7/31 125am!!!!! Adding L-glycine to the combination of essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine can further influence the effects on cannabis plants, as glycine is a non-essential amino acid with unique roles in plant physiology. Below, I outline the potential effects of L-glycine alone and in combination with the previously mentioned compounds, focusing on their impact on cannabis growth, stress response, and secondary metabolite production. Since specific studies on this exact combination in cannabis are lacking, I’ll integrate findings from plant science and infer synergistic effects based on known mechanisms. I’ll keep it concise and tailored to cannabis cultivation. ### Effects of L-Glycine on Cannabis Plants L-Glycine is a simple amino acid involved in protein synthesis, stress response, and metabolic pathways: - **Growth Promotion**: Glycine (50–500 mg/L) enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and rice, likely benefiting cannabis vegetative growth. It serves as a nitrogen source and supports protein synthesis, complementing EAAs and glutamine. - **Stress Tolerance**: Glycine acts as a precursor to glutathione, a key antioxidant, reducing oxidative stress from drought, salinity, or high light. In cannabis, it may improve resilience in challenging environments (e.g., outdoor grows or high-intensity indoor setups). - **Secondary Metabolism**: Glycine is a precursor to purines and chlorophyll, potentially enhancing photosynthesis and energy metabolism. It may indirectly influence cannabinoid and terpene production by supporting metabolic pathways, though direct evidence in cannabis is limited. - **Nutrient Uptake**: Glycine chelates micronutrients (e.g., iron, zinc), improving their availability, which is critical for cannabis in hydroponic or nutrient-poor soil systems. **Application Notes**: Foliar sprays or root drenches (100–500 mg/L) are effective, often applied twice weekly during vegetative growth or early flowering. ### Synergistic Effects with EAAs, Proline, TMG, and Glutamine Adding L-glycine to the mix may enhance the effects on cannabis plants in the following ways: 1. **Enhanced Growth and Biomass**: - **Glycine + EAAs/Glutamine**: Glycine complements EAAs and glutamine in protein synthesis and nitrogen metabolism. Studies show glycine (250 mg/L) combined with glutamine enhances shoot and root growth in plants, likely amplifying cannabis vegetative vigor and bud development. - **Synergy**: Glycine’s role in chlorophyll synthesis boosts photosynthesis, supporting the energy demands of EAA-driven protein synthesis and glutamine’s nitrogen assimilation, potentially increasing leaf area and yield. 2. **Improved Stress Resilience**: - **Glycine + Proline/TMG**: Glycine and proline both contribute to glutathione production, enhancing antioxidant defenses. TMG’s osmoprotectant properties further stabilize cells under stress. Together, they may improve cannabis tolerance to drought, heat, or salinity, maintaining growth in adverse conditions. - **Cannabis-Specific**: This combination could be particularly beneficial for outdoor cannabis or indoor setups with inconsistent temperature/humidity, reducing leaf wilting or nutrient stress. 3. **Secondary Metabolite Production**: - **Glycine + Glutamine/TMG**: Glycine and glutamine are precursors to metabolic pathways (e.g., GABA, purines) that may influence cannabinoid and terpene synthesis. TMG’s methyl donation could enhance these pathways by supporting enzyme activity. While direct evidence is sparse, this stack may increase terpene diversity or cannabinoid yield in cannabis buds. - **EAAs Contribution**: Methionine (an EAA) and glycine may support sulfur-containing compounds, potentially enriching terpene profiles. 4. **Nutrient Efficiency**: - **Glycine + EAAs/Glutamine**: Glycine’s chelating properties enhance micronutrient uptake, complementing EAAs and glutamine’s role in nitrogen assimilation. This could optimize nutrient use in hydroponic cannabis systems, reducing nutrient lockout. - **TMG Synergy**: TMG’s role in cellular hydration may improve nutrient transport, amplifying glycine’s effects on micronutrient availability. ### Potential Risks and Considerations - **Inhibition at High Doses**: High glycine doses (1000 mg/L) may inhibit growth by disrupting nitrogen balance, especially if combined with EAAs or glutamine. Studies on Arabidopsis show glycine can be toxic as a sole nitrogen source, so balanced mixtures are crucial. - **Nitrogen Overload**: Adding glycine to EAAs and glutamine increases nitrogen input, risking toxicity (e.g., leaf burn, stunted growth) in cannabis. Monitor for dark green leaves or clawing and adjust doses. - **Competition for Absorption**: Glycine, EAAs, and glutamine may compete for root or foliar uptake, reducing efficacy. Split applications (e.g., glycine in the morning, EAAs/glutamine post-light cycle) can help. - **TMG Interaction**: Glycine and TMG both influence methylation pathways, but excessive methyl donors could disrupt metabolic balance in sensitive plants. Stick to moderate doses (e.g., 100–500 mg/L glycine, 0.5–1 g/L TMG). - **Cultivar Variability**: Cannabis strains (e.g., sativa vs. indica) differ in nutrient and stress responses. Glycine’s benefits may be more pronounced in stress-prone sativas or nutrient-sensitive hybrids. ### Practical Recommendations - **Dosing**: Combine glycine (100–500 mg/L), EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) in a balanced foliar spray or root drench. Apply twice weekly during vegetative growth and early flowering. - **Application**: Use foliar sprays for rapid uptake during vegetative stages or stress events. Root drenches suit hydroponic systems. Test on a small batch to assess plant response. - **Monitoring**: Watch for nitrogen toxicity (dark, curled leaves) or stress relief (greener, more vigorous plants). Adjust concentrations based on cultivar and growth stage. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to maximize glycine’s chelating benefits. Ensure adequate magnesium for chlorophyll synthesis. - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient imbalances. In soil, maintain healthy microbial activity, as microbes naturally produce glycine and other amino acids. ### Evidence Gaps - **Specific Combination**: No studies address glycine combined with EAAs, proline, TMG, and glutamine in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid/Terpene Impact**: While glycine may support secondary metabolism, direct effects on THC/CBD or terpene levels are unconfirmed. Cannabis-specific trials are needed. - **TMG and Glycine**: Their combined effects on methylation in cannabis are speculative, as most TMG research focuses on other crops. ### Conclusion Adding L-glycine to EAAs, proline, TMG, and glutamine likely enhances cannabis growth, stress tolerance, and nutrient efficiency by supporting protein synthesis, antioxidant production, and micronutrient uptake. The combination may improve vegetative vigor, bud quality, and resilience to environmental stressors, with potential (but unconfirmed) benefits for cannabinoid and terpene production. Careful dosing and monitoring are essential to avoid nitrogen toxicity or absorption competition. Start with low doses, test on a small scale, and consult a cannabis cultivation expert for tailored advice. If you want me to search for more studies, focus on a specific aspect (e.g., glycine’s role in terpene synthesis), or provide application protocols for a particular cannabis strain, let me know!"""