Amazing strain. Did exploded in the last 3 weeks and fattened up even more to be great main colla about ten gram dried and cure and overall weight of 27 dry g.

Day 15 Today I switched the lights from the Seedling program to the Growth program. I measured around 640 PPFD at the canopy level so a bit high for early veg but I will leave it where it is now and keep an eye out for light stress, and raise the lights if I have to. I also added a second USB fan for increased circulation. USB fans are great when the plants are small and you don't want the stronger winds that the regular clip fan generates. I also started the clip fan but directed it towards the lights to help keep them a bit cooler. I adjusted the extractor fan from 60 secs Off/30 secs On to 30 secs Off/30 secs On in order to remove more hot air. This will have to be tweaked further and I'll monitor my temps to figure out a suitable setting. It becomes a balance between temp and humidity. I really wish I had a system where I could set a target VPD and then the system would automatically adjust temps and humidity to follow that target. Not practical in my small setup though but maybe one day... Temps are always an issue in my setup as I have two powerful lights inside one small tent without AC. I have set the light cycle so that the lights are on during the night but off during the day to help to balance the temp a bit. I'm also considering getting some extension cables and put the LED drivers outside the tent to see if that helps to bring down the temps a bit further. Later in the grow I will use CO2 so that will help a bit against higher temps. Finally, I watered the plants as they were getting a bit dry. The left plant got 1 liter and the right plant 1.5 liters as the sensor reported that that pot was a bit drier. I reckon it has to do with the plant being larger (almost double the size of the other plant!) I should also really get my ass in gear and hook up the Blumat system so I don't need to worry about hand watering. Day 16 Nothing to report really except some additional growth, both vertical and horizontal. Day 17 Watering time again. Gave them 6.2 pH water this time and once again more to the right plant as it drinks more. I guess around 1.3 liters for the left plant and 2 liters for the right one. I tried putting in my small USB humidifier into the tent but I soon realized that it can't share the USB power that the fans are running on so will have to get another USB power cable in there. Didn't expect that small humidifier to use that much power. Oh well, that's a job for another day. Day 18 The girls are thirsty it seems as I had to water again today according to the Blumat sensors (I really, really need to install my Blumat system as soon as possible but should get the last bits that I need in a day or two). I gave them 2.5 liters of water (pH 6.7) and once again a bit more to the right plant. I also was able to squeeze in my tiny humidifier into the tent even though the tomatoes are still in there. Turns out that all I had to do was to switch the USB power adapter from the old Samsung one that I used to a more powerful iPad charger. Now it works just fine to run the fan and the humidifier on the same power. The reason why I'm using a small USB humidifier instead of a proper one is simply due to the lack of space in my small tent. This (very basic) humidifier is the smallest one I could find yet have a decent size tank so I don't need to fill it up all the time. Day 19 The cover crop EXPLODED since yesterday so the first thing I had to do today was to cut that back a bit. I also started brewing some compost tea (AACT). I mixed together worm castings, rock dust, biochar, lime, bentonite, neem meal, kelp meal, and molasses and will leave it brewing for 24 hours and then feed it to the plants tomorrow. Would have liked to add alfalfa meal as well but haven't been able to find any. I usually put my seed starter warming mat under and taped to the brewing bucket to increase the tea temperature and microbial activity. The plants are doing their thing, a little bit taller and a little bit wider. I will probably top the right plant next week but not sure about the left one as it is smaller so might need another week. Day 20 The compost tea was ready today after 24 hours of brewing. I fed each girl with around 3 liters of tea (pH 6.8) and as I wasn't planning any foliar feeding this time around I kept the tea unfiltered. So that everything that went into making the tea ended up as a top dressing. I guess I don't have to water for a couple of days after this. Not much change is plant size but the leaves are filling out. Day 21 Today was completely uneventful so not much to report except that I probably will top the right plant tomorrow as it is up to 5 nodes.

Week 11 Update – Harvest Time Approaching! 🌱✂️ BioBizz#1 – BT51 She’s done! She hasn’t had any water for the past five days and will be harvested later this week. AN#4 – BT51 She still needs a little more time—probably another 1-2 weeks before she’s fully ready. AN#3 – BT47 Somehow, she’s managing despite the extremely close distance to the light. I haven’t held back on PPFD at all, but she’s handling it like a champ. Super curious to see what kind of yield she’ll bring in! 😅

I'm highly impressed with the autopots so far. It's very unlikely that I would have given the plants as much water as they have taken up and they would have suffered, as I was out of town for 3 days and they went through over 10gal while I was gone. Closed the valve on the autopots Fri evening and started up a batch of tea (about 2 gal) with kelp, alfalfa, bat guano, worm castings and, of course, Real Growers Recharge. I'll let the tea bubble for at least 24 hours and then split it between all 4 girls on Sunday just after topdressing and just before lights off. Autopots will get turned on at this time too. Everyone loved the change in light cycle and are starting to stretch nicely. Put up smaller squared trellis netting to spread out the bud sites as they continue to stretch. BBM1 is just a big, healthy girl and really think she's gonna give me a great harvest. BBM2 is filling out nicely and I think both the Cheese and Gruntz will need some tying down to keep the canopy as even as possible, even with the scrog. So anxious for the Blueberry smells to kick into gear.

Day 45 of Veg, middle of week 7. Today I made the Switch from LED Lights to 600W Dual Spectrum HPS. I'm using S&P 800CFM inline duct for Carbon filter and the 6inch cooltube i added to cool down the heat that this light bulb it spreading (29.1 C and keep on climbing up). i also installed the SCROG net (as u can see in pics) , broke one of the strands of one plant and turned it into supercrop, put some bandage on it and hope for it to heal. I truly hope everything will go as planed!

Day 120 The buds look dense and frosty, with swollen calyxes and visible trichome coverage-clear signs that the plant is nearing harvest. Some leaves are fading or curling slightly, which is normal at this stage as the plant directs its remaining energy into the buds. The environment appears stable, with reflective tent wo!!- controlled lighting, and monitoring equipment

We are down to the final week! Just 2 more watering days and then I'll be ready to cut them down, trim, and hang this weekend. It had been a long 21 weeks and I am not prepared for my next grow. Usually I like to run perpetually with my 2x4 tent, I should have had another set of 6 starting when I flipped these ladies into flower. That way when these finish I could have had another set ready to go in. But things came up and I am behind schedule and running out of nutrients. Honestly that is okay though because I am planning my whole year out. I'll buy the few items that I need such as PH Down, some ph pen calibrating powder, grab some more Flora micro and bloom, stock up on fabric pots, coco coir, and perlite. I'm even thinking of getting some new strains! Stay tuned for next week's harvest!

Obama runtz x gas tax they both are a little taller than all the seedlings as I'm smoking some.of the flower from last grow I'm seeing the more wild plants like this plants mother sister they smoke great but these 2 seedlings have a look to their leaves like some of the wild from the face off og from the gas tax Last grow I overwatered and had to toss them because growth got stunted and a bunch if fungus knats got to the soil. This time I'm trying promix potting soil wish I had tried promix hp but the store close by isn't a hudro store and that's what they had. So this time I'm being very cautious with the watering I'm only useing a spray bottle I'm currently giving 45 spray per solo cup every few days when it dries out. This promix soil seems to hold water decent so the 45 spray there is 0 water coming out the bottom the cup yet. Here soon I think I'm going to double the amount of spray to 90 and start letting the soil get dry dry before watering again. Probably will hold off on any nutes quite yet. I'm going from 24/0 to 20/4 light schedule this week. There getting to the point they won't strech as much is 4 hrs of darkness and I'm rasing the light intensity a little it's been around 150 I'm upping it to around 175. Most likely by the end of the week I'll probably be pushing 200 or close. Hopefully I can get them into the big tent soon At this stage I like to alternate having the fan blowing on them I feel it gives them time to recover and get stronger instead of wind stress. Also every few days I will turn the plants so they are facing away from the light making them grow back towards the light I fell it makes them stronger

Finally moving the gorilla cookie to the flowering room. Feels like work with this sore rib, but glad to have the transplant done and pray I wake up with it still alive lol. White LSD soon to follow and complete the 4-bucket DWC system…

I greet everyone. Planted seeds, sprouted 4 pieces. 2 do si 2 critical. 2 not yet. Added root, bacteria and some organic matter. Light 400 w, humidity 40-50%, temperature 25 C. gggggg

Kashi and craft blend top dress day 35

Buenas a tod@s!, bueno novena semana de flora de estás runtz x layer cake, seguramente ante última o última semana, les falta nada... La plata está hermosa, más allá de sus hojas marchitas tiene unos frutos super pretos, compactos, pesados y mucha resina... Tanbien ultima semana de nutientes. Aclarando q es mi segundo armario, y sigo teniendo algunos problemas q mejorar, siempre trabajando humildemente, planta humilde y muy hermosas, no tengo experiencia, escucho todas las opiniones, pero eso no me define, me encantan mis plantas y cada día trabajando para q sean mejor.. falta muy poco... Un saludo y buenos humos para tod@s!!! 💨💨🔥😎⚕️💪🏻 🇦🇷🤝🏻🇪🇦

COLOMBIAN JACK by KANNABIA Week #16 Overall Week #5 Flower This week she's doing good no issues she's getting some nice looking and smelling buds!! Stay Growing!! Kannabia.com COLOMBIAN JACK

These ones almost done getting frosty

9/15 12AM MONDAY!!! THEY ARE ALL DOING GREAT!!! VERY HEAVY AMINOS YESTERDAY, NO DAMAGE. 9/15 11:24PM. In plant cells, glutathione S-transferases (GSTs) act as transporters, facilitating the movement of anthocyanins from the endoplasmic reticulum (ER) to the vacuole for storage, though their exact mechanism is still being studied. The synthesis of glutathione (GSH) itself occurs in the cytosol of mammalian cells through two ATP-requiring enzymatic steps, while anthocyanins are synthesized via a separate phenylpropanoid pathway 9/15 11:42PM glutathione metabolism glutathione S-transferases plantsThinkingKicking off 5 searchesLooking at 71 sitesPutting it all together In plants, glutathione S-transferases (GSTs) are a diverse family of enzymes integral to glutathione (GSH) metabolism, primarily functioning in detoxification and stress response. The core of this process involves GSTs catalyzing the conjugation of the tripeptide GSH with various toxic compounds, or xenobiotics, making them less harmful and easier to sequester or transport. Glutathione (GSH) metabolism in plants GSH metabolism is a tightly regulated process that maintains cellular redox balance and protects against stress. Biosynthesis: GSH is synthesized in two adenosine triphosphate (ATP)-dependent steps:(gamma )-Glutamylcysteine synthetase (GSH1) joins L-glutamate and L-cysteine to form (gamma )-glutamylcysteine. In Arabidopsis, this enzyme is located exclusively in the chloroplasts.Glutathione synthetase (GSH2) adds glycine to (gamma )-glutamylcysteine to produce GSH. This enzyme is found in both the cytosol and the chloroplasts.Oxidation and reduction: GSH exists in two forms: a reduced monomer (GSH) and an oxidized disulfide dimer (GSSG).Oxidation: In the process of scavenging reactive oxygen species (ROS), GSH is oxidized to GSSG.Reduction: Glutathione reductase (GR) uses NADPH to reduce GSSG back into active GSH, which is essential for maintaining a high GSH-to-GSSG ratio inside the cell.Degradation: Glutathione can be degraded in the cytosol, apoplast, and vacuoles by (gamma )-glutamyltransferases (GGTs) and other peptidases, allowing for recycling of its amino acid components.Transport and compartmentalization: While synthesis occurs in the chloroplasts and cytosol, transporters move GSH and its conjugates throughout the cell, including to the mitochondria, peroxisomes, and vacuoles. Functions of glutathione S-transferases (GSTs) GSTs are a large, ancient gene family with diverse roles that extend beyond detoxification. The primary functions involving GSH are: Detoxification of xenobiotics and heavy metals: GSTs catalyze the conjugation of the thiol group of GSH to a wide range of electrophilic and hydrophobic substrates, including herbicides, pesticides, and heavy metals. The resulting GSH-conjugates are then recognized by ATP-binding cassette (ABC) transporters and sequestered in the vacuole, reducing their cellular toxicity.Protection from oxidative stress: Stress conditions like drought, salinity, and high light increase levels of ROS, which cause lipid peroxidation. 🚨Some GST isoforms have glutathione peroxidase (GPOX) activity, using GSH to reduce toxic lipid hydroperoxides to less damaging alcohols, thereby protecting cell membranes.Defense against pathogens: During pathogen attacks, GST activity increases to detoxify microbial toxins and secondary defense-related compounds. This helps to bolster the plant's resistance response.Metabolism of endogenous compounds: GSTs play roles beyond immediate stress response by participating in the normal metabolic processes of plants. Examples include:Hormone transport: Some GSTs act as binding proteins (ligandins) that transport plant hormones like auxins within the cell.Metabolite transport: Specific GSTs facilitate the transport of important compounds like flavonoids and anthocyanin pigments into the vacuole.Tyrosine degradation: The zeta GST class (GSTZ) catalyzes a critical step in the breakdown of the amino acid tyrosine.Redox signaling: By influencing the GSH/GSSG ratio and interacting with plant hormones, GSTs can regulate cellular signaling pathways that control growth and stress response. 🚨GSTs and plant stress response The expression of many GST genes is highly inducible by various environmental and endogenous cues, making them key players in a plant's ability to adapt to adverse conditions. Abiotic stress: Drought, salinity, heavy metals, and extreme temperatures all trigger increased GST expression, which helps plants manage resulting oxidative damage. Overexpression of specific GST genes has been shown to enhance stress tolerance in transgenic plants.Herbicide resistance: The ability of crops and weeds to detoxify herbicides through GST-catalyzed conjugation is a well-studied example of GSTs in stress adaptation. Herbicide safeners, chemical agents that protect crops from herbicide injury, work by boosting the activity of these GST enzymes.Developmental processes: Studies have linked the expression of certain GST genes to specific stages of plant development, such as fruit ripening, root and stem development, and flower formation. "" 9/16 12:32am AI Overview In plants, phenylalanine is the precursor for anthocyanin synthesis, a process that involves a multistep pathway in which glutathione (GSH) plays a critical role in the transport of the final pigment to the cell vacuole. Phenylalanine initiates anthocyanin synthesis The biosynthesis of anthocyanins begins with the amino acid phenylalanine, which is produced via the shikimate pathway. The conversion process, known as the phenylpropanoid pathway, involves a series of enzymatic reactions: 1. Phenylalanine ammonia-lyase (PAL): This enzyme catalyzes the first committed step of the pathway, converting L-phenylalanine into trans-cinnamic acid. 2. Downstream enzymatic reactions: A series of additional enzymes, such as chalcone synthase and chalcone isomerase, convert the intermediate compounds into unstable anthocyanidins. 3. Glycosylation: Glycosyltransferase enzymes stabilize the anthocyanidins by adding sugar molecules, creating the final, stable anthocyanin pigments. 4. Glutathione is crucial for anthocyanin transport After synthesis, glutathione is essential for transporting anthocyanins for storage. * Glutathione S-transferases (GSTs): These enzymes bind to the newly formed anthocyanins in the cytoplasm. This binding is a key step that facilitates the transport of the anthocyanin-GST complex across the tonoplast (the vacuolar membrane) and into the vacuole. * Vacuolar storage: Once inside the vacuole, the anthocyanins accumulate and provide color to plant parts, such as flowers, fruits, and leaves. * Catalytic role: Recent research has also found that certain GSTs have a catalytic role in anthocyanin synthesis. They can catalyze a dehydration step to form specific anthocyanidins, significantly boosting production in engineered systems. * The process in summary The synthesis and accumulation of anthocyanins involve a coordinated process of multiple steps: 1. Production from phenylalanine: The amino acid phenylalanine enters the phenylpropanoid pathway, where enzymes convert it into the basic flavonoid structures. 2. Formation of anthocyanidins: Further enzymatic modifications produce the colored but unstable anthocyanidins. 3. Stabilization and transport with glutathione: These anthocyanidins are stabilized by glycosylation and then bound by GSTs (which use glutathione). This binding enables their transport into the vacuole for storage and visible pigmentation. 9/15 12:46AM https://pmc.ncbi.nlm.nih.gov/articles/PMC7238016/ 9/16 1:30AM BKO is looking great!!! Conclusion The “butter frosting” resin on Cookie Apple F1, healthy yellow-green fusiform, and Blueberry KO’s pigmented cotyledons show your anthocyanin-glutathione-phenylalanine strategy is working—phenylalanine drives synthesis, glutathione ensures transport. Tweak amino acids to 100–150 mg/L to reduce tip burn. 9/16 3:34am 9/16 4:31AM Anthocyanin glutathione synthesis phenylalanine proline tmg powder relating current project: * Phenylalanine is a precursor: Phenylalanine is an amino acid and the starting point for the phenylpropanoid pathway in plants. * Anthocyanin synthesis: This pathway creates various secondary metabolites, including the flavonoid pigments known as anthocyanins, which give plants their red, purple, and blue colors. * Pathway activation: Multiple enzymes, such as phenylalanine ammonia-lyase (PAL), catalyze the conversion of phenylalanine into the building blocks for anthocyanin. * Anthocyanin and glutathione synthesis * Glutathione S-transferase (GST): This enzyme is crucial for synthesizing anthocyanins in plants. It transports anthocyanins into the cell's vacuole for storage. * Glutathione (GSH) production: Anthocyanins can promote glutathione synthesis in certain cells. For instance, the anthocyanin cyanidin-3-O-β-glucoside (C3G) has been shown to increase the expression of glutamate-cysteine ligase (Gclc), a key enzyme in GSH synthesis. * Antioxidant effect: By upregulating GSH production, anthocyanins contribute to the antioxidant defense system, protecting cells from oxidative stress. * Proline and glutathione synthesis * Shared precursor: In some organisms, the synthesis pathways for proline and glutathione share a precursor molecule called γ-glutamyl phosphate. * Alternative pathway: Research on bacteria and yeast has revealed an alternative, trace pathway where γ-glutamyl phosphate from the proline synthesis pathway can be diverted to produce the glutathione precursor γ-glutamylcysteine. * Oxidative stress response: Studies in mouse oocytes show that supplementing with proline upregulates genes related to glutathione synthesis (Gclc and Gclm), increases glutathione levels, and reduces oxidative stress. * TMG powder, methylation, and homocysteine * Methyl donor: Trimethylglycine (TMG), or betaine, is a potent methyl donor, meaning it provides methyl groups needed for various biochemical processes in the body, including the methylation cycle. * Homocysteine regulation: One of TMG's primary functions is to convert the amino acid homocysteine into methionine. This helps regulate homocysteine levels, which is important for cardiovascular health. * TMG and proline interaction: In plants and some organisms, TMG and proline act as compatible solutes or osmoprotectants, helping cells stabilize against osmotic stress like drought or salinity. However, in human biology, TMG mainly functions through methylation, while proline is involved in different metabolic and antioxidant roles. * How they all relate The listed components are connected through several overlapping metabolic and regulatory pathways: * Anthocyanin synthesis starts with phenylalanine. * Anthocyanins can promote glutathione synthesis via upregulation of key enzymes like Gclc. * Glutathione synthesis can be influenced by the proline synthesis pathway, as they share an intermediate in some contexts. * TMG powder supports the methylation cycle, which helps regulate homocysteine levels. While TMG and proline serve similar protective roles in some organisms, their primary human metabolic functions differ, with TMG focusing on methylation and proline having distinct roles in antioxidant response and metabolism 9/17 217am Die Hard Christmas Grow 9/18, 11:34 AM. I ordered some square saucers that were cartoonishly too small but they fit inside the AC infinity germination kit and they fit with the Bud Cups perfectly really nice so it’s not a total loss. 9/18 11:45AM mix. Foliar Spray, the rest of the mix ec 0.46 Mixed up Aminos first and separate and use 16 oz for foliar spray. Then mixed up: Root: 1 mL/L Connoisseur A & B GROW, .2 mL/L CaliMagic, .2 mL/L Purpinator. Setria Glutathione: 150 mg/L(Brand: Emerald 250mg capsule.) TMG: 150 mg/L = (Brand Nutricost) Phenylalanine: 150 mg/L (Brand Nutricost) Proline: 150 mg/L (Brand Nutricost). 9/18 228PM AI Overview Glutathione influences plant colors by regulating the accumulation of pigmented compounds, primarily anthocyanins. The tripeptide accomplishes this through its role in transporting pigments within plant cells and in protecting against environmental stresses like UV radiation that can cause oxidative damage. Transporting pigments into plant cell vacuoles Glutathione works with a class of enzymes called Glutathione S-transferases (GSTs) to transport pigments like anthocyanins into the vacuole for storage. Anthocyanin transport: In plants with pigmented tissues, such as purple grapes or red flowers, glutathione-conjugated pigments are transported by GSTs across the tonoplast membrane into the vacuole. This process is crucial for the stable accumulation of pigments. Genetic manipulation: Research shows that manipulating specific GST genes can alter a plant's pigmentation. For instance, silencing a particular GST gene in purple grape hyacinths caused their petal color to shift to a lighter shade of purple. Similarly, defective GST genes in carnations resulted in pale-colored flowers. Protecting against UV light and stress Glutathione helps regulate plant pigmentation in response to environmental factors, especially UV-B radiation. Activating flavonoid production: When plants are exposed to UV light, a surge in glutathione triggers the expression of genes involved in producing flavonoids. Flavonoids, including anthocyanins, can act as protective sunscreens for the plant, and their increased synthesis and accumulation can alter visible coloration. Balancing oxidative stress: Intense UV-B radiation increases reactive oxygen species (ROS) in plants, which can cause oxidative damage. Glutathione is a master antioxidant that helps detoxify these ROS, preventing cellular damage that can affect a plant's pigment-producing mechanisms. Indirectly influencing plant colors By regulating cellular redox status and interacting with other molecules, glutathione also affects pigment expression in more indirect ways. The xanthophyll cycle: As part of a plant's antioxidant system, glutathione helps maintain the reduced state of other protective antioxidants like tocopherol and zeaxanthin. Zeaxanthin is a carotenoid pigment involved in the xanthophyll cycle, which helps dissipate excess light energy. Redox signaling: The balance between reduced glutathione (GSH) and oxidized glutathione (GSSG) is a key cellular signal for stress response. A shift in this ratio during environmental stress can influence the production of secondary metabolites like pigments, allowing the plant to adapt. " 9/19 1:41AM AI Overview The key difference is that anthocyanins are the sugar-containing form (glycosides) of pigments, while anthocyanidins are the sugar-free form (aglycones). Anthocyanidins are the foundational molecules, and when a sugar molecule attaches to them, they become anthocyanins, which are more stable and water-soluble, making them the forms found naturally in plants, such as berries and purple vegetables. Anthocyanidin (Aglycone) Structure: The basic, sugar-free molecule of the anthocyanin structure. Location: Not found freely in nature but is the core component that is then glycosylated. Properties: Color changes with pH, being visible in acidic conditions but colorless in basic conditions. Examples: Cyanidin, delphinidin, pelargonidin, peonidin, petunidin, and malvidin. Anthocyanin (Glycoside) Structure: Consists of an anthocyanidin linked to one or more sugar molecules. Location: Found in the vacuoles of plant cells. Properties: Water-soluble and are the pigments responsible for the red, purple, and blue colors in plants. Function: The sugar attached provides stability, allowing for accumulation in plants and providing antioxidant properties. Examples: Cyanidin-3-glucoside and other derivatives like acylated anthocyanins. " 9/19 2:43AM I also saw a good mans immediate accent into Heaven, that perspective matters too. no less angry about it though. 9/19 10AM Heavy Amino spray 250mg each in 1L of Setria Glutathione and Phenylalanine 9/19 10:10PM The sons and daughters of americas real terror organization carried out the last horror show, and the one before that and before that. It's not a foreign country, it's always the anti white anti human black sheets and badges that did this. 9/19 10:43PM AI Overview Phenylalanine and glutathione contribute to plant colors through different biochemical pathways . Phenylalanine is a precursor for the pigments themselves, primarily anthocyanins, while glutathione is involved in the transport and stabilization of these pigments within the plant cell. Phenylalanine: The pigment precursor The source of aromatic compounds: Phenylalanine is an aromatic amino acid and the starting compound for the phenylpropanoid pathway in plants. This pathway produces a vast number of secondary metabolites, including anthocyanins, which give many plants their red, purple, and blue colors. Color enhancement: Research has shown that increasing the amount of available phenylalanine can lead to more intense red coloration in some fruits, such as mangoes and apples. Pathway stimulation: Phenylalanine ammonia-lyase (PAL) is a key enzyme in this pathway that converts phenylalanine into precursors for anthocyanin biosynthesis. An increase in phenylalanine levels stimulates this entire process. Glutathione: The pigment transporter Anthocyanin transport: After anthocyanin pigments are synthesized in the cell's cytoplasm, they must be transported into the central vacuole for storage. Glutathione S-transferases (GSTs) are a family of enzymes that facilitate this process. Color intensity: A functional GST is essential for proper anthocyanin accumulation. Mutations in GST genes can result in a significant decrease in color intensity, as seen in the pale or white flowers of certain gentian mutants. Mechanism of action: The GST binds to the anthocyanin pigment and moves it from the cytoplasm into the vacuole. If the GST is defective, the pigment cannot be transported correctly, leading to a loss of coloration. Summary of interactions The roles of phenylalanine and glutathione are distinct but cooperative in producing plant colors: Phenylalanine provides the essential building blocks for creating the colored compounds (anthocyanins). Glutathione, with the help of GSTs, ensures these colored compounds are correctly moved to their storage location (the vacuole). This cooperative system explains why mutations or deficiencies in either process can lead to reduced or altered coloration in plants." 9/19 10:51PM AI Overview. Trimethylglycine (TMG) supports the production of glutathione, a vital antioxidant . However, there is no evidence to suggest a direct relationship between these supplements, the amino acid phenylalanine, and the colors of plants. The concepts are linked indirectly via complex biological processes. Trimethylglycine (TMG) and glutathione TMG and glutathione are connected through the body's methylation cycle. TMG as a methyl donor: TMG plays a critical role in the methylation process by donating a methyl group, which helps convert the harmful amino acid homocysteine back into methionine. Support for glutathione production: This methylation cycle, which is supported by TMG, is essential for producing glutathione. Glutathione is a powerful antioxidant that protects against cellular damage and is crucial for detoxification. Supplementation considerations: TMG supplements are sometimes taken alongside other supplements to support health, though consultation with a healthcare provider is recommended. Phenylalanine and the methylation cycle Phenylalanine is an essential amino acid, but its role is distinct from the TMG-glutathione process. Essential nutrient: Phenylalanine is a key nutrient for cellular metabolism. Potential interactions: A separate medical study on experimental hyperphenylalaninemia (abnormally high phenylalanine levels) in chicks observed a decrease in other amino acids in the brain, including those involved in the glutathione pathway. This demonstrates how excessive levels of one amino acid can potentially influence others, though this does not represent a typical interaction. Plant colors The connection between the supplements and plant colors is purely conceptual, as the colors are determined by completely different biological processes. Anthocyanins: The colors of many plants, including red, purple, and blue flowers, come from pigments called anthocyanins. Glutathione in plants: While plants contain glutathione as an antioxidant to combat stress, it influences color by regulating the transport and accumulation of anthocyanin pigments, not by being a pigment itself. TMG and phenylalanine in plants: Plants contain TMG, which functions as an osmoprotectant (protecting against osmotic stress). They also contain phenylalanine, but these substances do not directly determine the plant's visible color." 9/19 11:21PM. !!!!!! this was pointless and im dumber for having read it. !!! Light intensity and spectrum affect metabolism of glutathione and amino acids at transcriptional level: https://pmc.ncbi.nlm.nih.gov/articles/PMC6938384/ 9/20 11:08 AM the seedlings and the four autos are doing just great. The amino spray with phenylene and glutathione really had nice effects no burning nice solid growth even seedlings from basil lavender various lettuce all are perfect.🚨🚨🚨👍👍👍👍👍 9/21 2AM I AM BECOME ANTHOCYANID!!! ITS WORKING AND ON A SEEDLING I SEE THE GELATO COLLORS IN BLUEBERRY KO AND THE LEAF SHAPE OF BUBBLES BLUEBERRY,!!!

It’s been a while since last update and apologies , 3x dos si dos 33 in auto pots 1x dos si dos in all mix soil and hand watered 1x peyote critical auto pot 3x cookie dough sundae auto pot but the dos si dos 33’s are tanking along and proving very well , lovely Aromas so far , there’s a nice sharp sour lime flavour to it . Few weeks to go still and hoping they keep on flourishing as they are , this lot is from seeds so it also looks like a have couple of pheno’s of the strain . Buds are forming very well and growing big and dense. Peyote critical is stacking up nicely and doing well as always , sweet and earthy notes so far . Great structure to the buds also Cookie dough sundaes have been a challenge to deal with as I have very little info on this strain but after they seem slow to chunk up through mid flower they are finally making some gains and looking very sativa like with the shape of bud and going to need 10-11 weeks on this which I wasn’t quite wanting or expecting but it is what it is , but saying that the flavours from this is super sweet and tangy and really look forward to this new flavour to hit on .

Good week this week. The plant is growing and growing. I can’t complain. One more week and then I will switch the lights to 12/12. See you next week!

Week 7 is going smoothly so far. The flooding issue has subsided and the clones seem to be really taking off now. I'm excited to get back from vacation and really see my girls and my reservoir conditions after a 2 week hiatus.

Still having an issue with watering. They don't seem to be drinking as much as I thought... Sometimes it's 36-48 hours between waters, and yes I am watering til a little runoff dribble out.