test for video uploads

(Days 28-35) (Day 29) Everything seems to have recovered fine from topping so I transplanted into their 1.7 gallon pots. They were pretty rootbound so this should make them nice and happy. Going to start LST after recovery. Got a ton of holes poked around the rim of the bucket which should make tying down easier. I’ve been asking around on some forums and reading some articles and I’ve decided to switch out my MH bulb for the included HPS bulb. Apparently HPS bulbs will stretch the nodes out quite a bit more and provide higher PAR value for the same amount of electricity. My plants are looking pretty small for day 29. Although, that could just be from stuffing 15 plants under one 250-400w light. I’ve been powering the lights up to 400w at night and back down to 250 during the day. 400 just gets it too hot during the day (86-88) without ventilation. That temperature should be fine in veg once my co2 has really built up some mycelium. (Day 32) Just got 14/15 topped. The BCTG runt is just going to be lst’d and not topped or fimmed. I decided to just fim the plants I started later so they’d have a bit more time to recover and catch up to the others. I also LST’d all 9 quite well. Almost had a stem break but she’s doing just fine now. The deformed bbb was accidentally fimmed and as a result it’s got a very trippy looking main stem that has branched off into 6 mains. I definitely have fungus gnats. Fuckers must have got in with that coco I bought from hydro store. This has never happened to me with just promix but the benefits of coco are too much to pass up. I might order an OG BioWar/capsbennies/grubgrenade to help naturally get rid of them. I’m getting some confirmed yellowing on bottoms of the zkittlez and WW’s. They must be heavy feeders because no other plant in the tent is getting yellow like that. I’m going to feed a compost tea, top dress everything at full strength and mulch in some worm castings to help break it down faster. Will also do a foliar spray with some insect frass, microbes and m1pps. (Day 33) Got some more of that marine phytoplankton stuff to help stabilize my RO water. It’s got a good amount of soluble magnesium and trade minerals in it so that should help stop the yellowing on the zkittlez and WW. (Day 34) Things are looking great so far and they’re all recovered from topping/transplant/lst and thriving. Some slight yellowing and a few of the plants are cupping from heat and high humidity. I just put in a dehumidifier to help bring the rh down. The m39/zkittlez bagseed plant is just outpacing everything in the tent by 4-6 days. No wonder organized crime used to grow it. I hear it can completely flower in 45 days with a solid yield and very little trimming or leaf matter. It’s very weak on the high and smell though. Nonetheless it should be nostalgic to try those old terps. There is still a small chance that it’s a super vigorous zkittlez plant too. Plants should be showing sex this coming week. Hoping for 8+ fems but I can make do with just 4. 1-3 is unlikely but that would piss me off beyond belief. I’ve heard of it happening though.

Week 9 of Critical RQS Day 57 FLUSH WEEK!!!!! I have decided to flush the critical. Normally the critical needs 7 to 9 weeks of flowering and on week 9 . I don't see a lot of white pistils anymore and tricomes are all cloudy and some ambers it's going to be a exiting week. Day 58 Flush the plants and run off had a lot of nutrients in it so have to flush for quit a bit Day 59 Drying the soil out some and then do big flush again hopefully i will get those nutrients out other then that critical is looking awesome 🤘🏻😎🤘🏻 Day 60 Flush them again today and getting the nutrients out of the soil I probably have flush at least for a week i had bin a bit lazy checking with EC meter and now i have to deal with it i don't like to be inconsistent but is was a bizzy week Day 61 Sunday rest day nothing going on 🤘🏻 Also want to thank everybody for the comments likes etc you know who you all are 👍🏻😍👍🏻 visit www.marshydro.eu for your best gear and use the PROMO CODE: DEVILSBUD Fully marshydro supported Setup: Marshydro GT100X100X180 Marshydro Ts 1000 LED lamp soil :Plagron batmix Nutrients :Advanced Nutrients Advanced Nutrients pH Perfect Bloom Advanced Nutrients pH Perfect grow Advanced Nutrients pH Perfect micro Advanced Nutrients B-52 Advanced Nutrients Voodoo Juice Advanced Nutrients Rhino Skin Advanced Nutrients Big bud Advanced Nutrients Bud candy Advanced Nutrients Overdrive Advanced Nutrients flawless finish Advanced Nutrients Sensizym BioBizz - Calmag Mars Hydro tent handy info Lock All Light Inside: MARS HYDRO Grow Tent Interior is made of diamond mylar. Non-toxic to the environment, no harm to plants. High-quality 1680D canvas being double stitched, (ALL OTHERS BRAND grow tent is 601D canvas) which is tear-proof for perfect light locking, No light leaks or rips at all. Durability Grow Tent :Stable Metal corner adapters and poles are the trustful supporters of the tent. No rusting nor paint-falling. Quick easy tool-free installation. Carries strong heavy-duty SBS zippers, double layer lining to create a light-proof seal. Easy Observation of your Plants: Special peek👀 window made by double layers of cloths. Observe the growing conditions of your plants without opening the tent and avoid disturbing plants. The peek window also serves to dissipate heat and ventilate air. Removable Tray Keep Clean: The package includes a removable waterproof floor tray to hold soil and fallen leaves. The tray can be taken out easily to wash. Removable and water-proof cleaning easy. Good Ventilation&Fast Assembly: Circular double-sleeved vent holes with adjustable nylon strap for air circulation by exhaust fan, ducting, carbon filter, or reflector. Better allows proper light, heat, and airflow. Quick tool-free installation. Reinforced by a sturdy metal frame to ensure security and stability, supports up to 140lb. Perfectly safeguard your plants to thrive even in winter.

April 10th, Time to repot! First, the new pot, this will be the final pot for my White Widow, a 50L geotextile pot with a substrate I prepared and amended beforehand. I kept a diary about the reciepe of this substrate! Before transplanting, I added about 75g of BioGrow as a top dressing to provide a good nitrogen boost for the next few weeks. Then I had to select one of my three White Widow plants. I applied a spoonful of TNC Mycorrplus to the slightly damp roots so it would adhere well. I placed it in the center of the pot and watered like 3L with Bioenhanceur, trying to be gentle during this steps Now, fingers crossed for what comes next...🤞 Thanks for stopping by!💪💚

**Encontrarás la traducción a español al final de la descripción** From/Desde: 22/03/19 || To/Hasta: 28/03/19 From day/Desde día: 29 || To day/Hasta día: 35 You can find the Money Maker Diary here: ** Podéis encontrar el diario de las Money Maker aquí:** https://growdiaries.com/diaries/25667-gorillamakingmoney-gorilla-vs-money-m -----IMAGES & VIDEOS----- Video 1: Exactly the same video as 2 but accelerated +400% to fix it to 1 minute for instagram. Video 2: 7 days (29~35) TimeLapse, 1 image each 60 secs @ 30 fps Video 9: An overview video not as awesome as it's music 😂💪 Video 10: Myself working on the babies, lowering tops, topping & training. Video 12: Full working on the secondary Gorilla, twisting some branches, topping & training. -----WEEK SUMMARY----- Well, now i'm getting a bit scared as i adventured myself into Photoperiod, high training, full topping... in indoors at once. I feel that plants are going good, but i really don't know what am i doing, and if i'm doing well. Also i don't know how much time should i give them of vegetation stage, because i don't want to eternalize myself with this tent, so i'm planning to leave this upcoming 6th week and maybe the 7th of vegetation, but no more. Anyway they look pretty atm 😻. In video 10 the main-linning Gorilla suffered an accident as i grabbed the main left branch from a weak point and it has broken (sec 39 aprox.) so it has one less node than the right branch. -----WATERING CALENDAR----- 24/03/19 - 1.000 ml with all week nutrients @ 0.9 E.C. PH5.8 *****ESPAÑOL***** -----IMÁGENES & VÍDEOS----- Vídeo 1: TimeLapse, exactamente el mismo vídeo que el 2 pero acelerado a un poco más del 300% para ajustarlo a 1 minuto para Instagram. Vídeo 2: 7 días (29~35) TimeLapse, 1 imagen cada 60 segundos @ 30 fps. Vídeo 9: Un vídeo general no tan increíble como la música de fondo 😂💪. Video 10: Trabajando en las pequeñas, rebajando las alturas, haciendo topping y entrenando. Video 12: Trabajando a pleno en la segunda Gorilla, girando algunas ramas, haciendo toppings y entrenándola. -----SUMARIO SEMANAL----- Bueno, ahora estoy empezando a estar asustado. Me he aventurado a probar con fotodependientes, entrenamiento fuerte y super topping, todo a la vez. Siento que las plantas van bien, pero realmente no se lo que estoy haciendo ni si lo estoy haciendo bien. También tengo dudas sobre cuanto tiempo más dejarlas en estado vegetativo ya que no me quiero eternizar con este armario, por lo que estoy planeando dejar esta sexta semana de crecimiento y quizás una séptima, pero no más. Aun así y con todo, las nenas se ven preciosas 😻. En el vídeo 10 sufro un accidente en la Gorila con main-linning ya que amarré el final del tallo principal izquierdo por un punto débil y este se partió (sec 39 aprox.), por lo que tiene un piso de topping de más en comparación con tallo derecho. -----CALENDARIO DE RIEGO----- 24/03/19 - 1.000 ml con todos los nutrientes semanales @ 0.9 E.C. PH5.8

Shoot!!!! They’re beginning to thickening up, i’ve done some lst for 5 days and now they’re pretty good. I’m letting them grow and will begin to tie the other branches when they will be big enough!

This was the first time using the LED panels, they have much better canopy penetration than HID. I started at 12 inches, the leaves showed signs of light stress. The lights were moved back 6-8". Fan leaf growth rate is averaging 1/4" a day. 18"H x 6"W.

Everything is going very well as expected, good progress. I had the transplant on the last day of the first week..✌️🌱

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Posting the phenos when I do them

This Canuk lady has been a GEM! It’s just about time to cut this girl down. She’s been a very nice plant to grow! I’ve just been giving her plain water for awhile now so she is yellowing out and fattening up! She will be coming down this weekend I’m guessing 😀 I will definitely run this genetic again! Pure gold! 😎🍿

great strain. got a beautiful Pheno. I'll update pics and taste after dry but my impatient smoke says it tastes good. sweet seeds is definitely a seed bank for the home grower

Gracias al equipo de Seedsman y XpertNutrients sin ellos esto no sería posible. 💐🍁 Bubba Cheescake: Bubba Cheesecake es un cruce de Bubba Kush anterior al 98 con Cheese Cake (Wedding Cake x Exodus Cheese). Se trata de un híbrido 70% índica con muy buenos rendimientos, alto en THC y bajo en CBD. Bubba Cheesecake prospera en interiores y exteriores. En interior se aconseja un corto periodo de crecimiento vegetativo debido a la cantidad de estiramiento que presentan las plantas. Las plantas pueden crecer hasta una altura de 250 a 350 cm. cuando se deja crecer naturalmente al aire libre. En interior, la floración dura entre 60 y 65 días, con rendimientos entre buenos y altos, de 400 a 550 gr/m2. Las plantas de exterior son capaces de producir entre 700 - 800 gr. peso seco. En las latitudes norteñas, en exterior, los productores pueden esperar cosechar a principios de octubre. Los cogollos son grandes y duros como piedras. Las plantas maduras muestran atractivos colores púrpuras y azules y brillan con una resina pegajosa que cubrirá las manos del cultivador si no tiene cuidado. El componente Wedding Cake agrega dulzura al sabor del regaliz y el aroma es terroso y musgoso. La producción de THC es muy alta con un nivel bajo de CBD. El efecto es intensamente narcótico, fuerte y duradero. 🌻🚀 Consigue aqui tus semillas: 🍣🍦🌴 Xpert Nutrients es una empresa especializada en la producción y comercialización de fertilizantes líquidos y tierras, que garantizan excelentes cosechas y un crecimiento activo para sus plantas durante todas las fases de cultivo. Consigue aqui tus Nutrientes: https://xpertnutrients.com/es/shop/ 📆 Semana 12: Ultima semana de crecimiento antes de pasar a floración tras el solsticio de verano. He aprendido mucho sobre el cultivo de exterior, el próximo año será diferente.... Continuo con las dosis de fertilizantes recomendada por el fabricante. 📆 Semana 12: Ultima semana de crecimiento antes de pasar a floración tras el solsticio de verano. He aprendido mucho sobre el cultivo de exterior, el próximo año será diferente.... Continuo con las dosis de fertilizantes recomendada por el fabricante.

Las 4 nenas del scrog iniciaron con el ciclo 12/12 el día 05/11/2021: están siendo alimentadas con advanced nutrients(dosis indicadas en la foto de la tabla) -micro -blom -grow -b52 -rhino skin -big bue -bud ignitor -bud candy -sensi cal mag xtra Las 2 nenas en macetas de 30LT 18/6: por el momento están siendo alimentadas con advanced nutrients(dosis indicadas en la foto de la tabla) -micro -grow -blom -b52

She’s still stretching! This week I removed Voodoo Juice, Tarantula and Piranha, and added B-52 and Bud Factor X (never tried this one before). Since the princess loves this 2ml/L dosage for NPK nutes, I’ll not be touching that for the moment. That seems to be the optimal dosage for a sativa like this. Very nice!

7/28 1220AM MONDAY.... Bubbles are looking ok, I need to trim them back to 4 colas, I am really stressing them with small space limitations, and temps at 80 to 81 7/29 Tuesday 5:45 AM💦💦 6:15 AM water with methylene blue two pills of nitric boost amino acid, five drops of Quick Sorb and I added bud factor X today and a quarter mL per liter of rhino silica💦💦💦. 1030 AM I don’t know what I’m gonna do. It turns out the bubbles plants are terrible. The stems are all very weak and they’re growing bushy at the top. I didn’t get them separated so all of the side branches grew up really long it’s completely useless. It is absolute this plant. It will be thrown in the garbage. Can you fucking believe I have to throw another entire plant in the fucking garbage😡😡😡😡. TREVOR plant cut down to 2 colas only, very top heavy not liking my grow space, had 2 more than I wanted so it's all fucked.... will remove shitty small colas and get maybe 2 good colas per plant, not expecting much. 1219pm....HOLY SHIT I FUCKED THESE.....TOOK OFF SOME COLAS.....NOT ENOUGH SPACE AND I PUSHED THEM TOO FAR.....I CAN FIX IT 7/30 1215am found a GREAT science link::: ""https://www.frontiersin.org/research-topics/8125/cannabis-genomics-breeding-and-production/magazine"". 345am PROLINE WILL BE HERE TODAY.ALL RESEARCH INDICATES PROLINE WILL PROTECT FROM HEAT STRESS!!!! 11:30 AM they are good again they are recovering from heavy defoliation half of the plant was taken off of one of them. I pushed it a little far but today they are recovering from heat and lack of air and lack of space the leaves are reaching up and they are not folding under heat stress. They look good today. Fast recovery heavy amino yesterday.👍👍👍👍🚨🚨🚨🚨 🚨🚨🚨🚨🚨🚨🚨🚨 GOT THE AMINOS AND HERE IS THE GROK RESULT OF MY MIX.......🚨🚨🚨🚨🚨🚨🚨🚨 The effects of applying essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine to cannabis plants can be significant, as these compounds act as biostimulants, influencing growth, stress resilience, and secondary metabolite production. The available research, primarily from studies on amino acids in plants, including cannabis, provides insights into their roles, though specific data on this exact combination is limited. Below, I summarize the effects of each compound on cannabis plants, drawing from relevant studies, and infer potential synergistic effects based on their known roles in plant physiology. Note that TMG (betaine) has less direct research in cannabis, so some effects are extrapolated from other plants. ### 1. Essential Amino Acids (EAAs) EAAs (e.g., leucine, lysine, methionine, etc.) are critical building blocks for proteins and play roles in plant metabolism and stress response. In cannabis: - **Growth and Development**: EAAs enhance protein synthesis, supporting shoot and root growth. Foliar or soil application of EAAs (e.g., 150–300 mg/L) increases leaf chlorophyll, shoot biomass, and root development in various plants, likely applicable to cannabis.[](https://www.nature.com/articles/s41598-022-21273-6) - **Nutrient Uptake**: EAAs like methionine and lysine improve nutrient absorption (e.g., nitrogen, iron) by acting as chelators, potentially enhancing cannabis nutrient efficiency in hydroponic or soil systems.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) - **Stress Mitigation**: EAAs, particularly methionine, support antioxidant production (e.g., glutathione), reducing oxidative stress from drought, salinity, or high light, common in cannabis cultivation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2020.00928/full)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cannabinoid/Terpene Production**: Limited evidence suggests EAAs may influence secondary metabolism. For example, methionine could enhance sulfur-containing compounds, potentially affecting terpene profiles in cannabis.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) **Application Notes**: EAAs are often applied via foliar spray (150–300 mg/L) or soil drench. Overuse may lead to nitrogen imbalance, so moderation is key.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### 2. Proline Proline is a non-essential amino acid that accumulates in plants under stress and serves multiple functions: - **Stress Tolerance**: Proline acts as an osmolyte, stabilizing cell membranes and scavenging reactive oxygen species (ROS) during drought, salinity, or temperature stress. In cannabis, exogenous proline (e.g., 50–100 mM) may enhance tolerance to environmental stressors, improving resilience in outdoor or controlled environments.[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW)[](https://pmc.ncbi.nlm.nih.gov/articles/PMC3548871/) - **Limited Growth Effect**: Proline alone is a poor nitrogen source for growth. Studies on Arabidopsis show it has a neutral or inhibitory effect on growth when used as the sole nitrogen source, suggesting it’s better for stress response than biomass accumulation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Cannabis-Specific**: Proline helps cannabis maintain water balance, potentially improving resistance to extreme temperatures, which is critical for outdoor grows.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) **Application Notes**: Foliar or root application (50–100 mg/L) is effective for stress mitigation. High doses may inhibit growth if not balanced with other nutrients.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full)[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW) ### 3. Trimethylglycine (TMG, Betaine) TMG is less studied in cannabis but known as an osmoprotectant and methyl donor in other plants: - **Stress Protection**: TMG stabilizes proteins and membranes under salinity, drought, or temperature stress, similar to proline. It may protect cannabis plants in harsh conditions, maintaining photosynthetic efficiency.[](https://www.mdpi.com/2311-7524/9/7/807) - **Methylation Support**: TMG donates methyl groups, potentially enhancing secondary metabolite synthesis (e.g., cannabinoids, terpenes), though direct evidence in cannabis is lacking. Its role in methylation could support metabolic pathways linked to EAAs and glutamine. - **Growth Effects**: Limited data suggest TMG may improve chlorophyll content and photosynthesis, indirectly supporting biomass in cannabis. **Application Notes**: TMG is typically applied at 0.5–2 g/L via foliar spray. Its effects in cannabis are inferred from other crops, so careful experimentation is needed. ### 4. Glutamine Glutamine is a conditionally essential amino acid central to nitrogen metabolism: - **Growth Promotion**: Glutamine (10–1000 mg/L) significantly enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and hibiscus, likely benefiting cannabis vegetative growth. In cannabis, it may support lush foliage and robust rooting in hydroponics.[](https://www.scielo.br/j/oh/a/GZxMgqXb9wm7bsCTv4WwPdc/?lang=en)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html?lang=en) - **Nitrogen Assimilation**: As a primary nitrogen acceptor, glutamine improves nitrogen use efficiency via the glutamine synthetase/glutamate synthase cycle, enhancing nutrient uptake in cannabis.[](https://academic.oup.com/jxb/article/58/9/2339/544408)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Stress Response**: Glutamine supports proline synthesis, boosting stress tolerance. It also contributes to chlorophyll and protein synthesis, improving photosynthesis and yield potential in cannabis.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Secondary Metabolism**: Glutamine is a precursor to glutamate, which influences GABA, arginine, and proline synthesis, potentially affecting cannabinoid or terpene production indirectly.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) **Application Notes**: Foliar sprays (250–1000 mg/L) or root application twice weekly are effective. Glutamine at 250 mg/L combined with other amino acids (e.g., glycine) shows synergistic growth effects.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) ### Synergistic Effects on Cannabis - **Growth and Yield**: The combination of EAAs, glutamine, and proline may enhance vegetative growth and biomass. Glutamine and EAAs drive protein synthesis and nitrogen uptake, while proline supports tissue integrity under stress. TMG likely amplifies these effects by improving cellular hydration and methylation, potentially increasing leaf area and bud quality.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.nature.com/articles/s41598-022-21273-6) - **Stress Resilience**: Proline and TMG act as osmoprotectants, while EAAs and glutamine bolster antioxidant systems, collectively improving cannabis resilience to drought, salinity, or temperature extremes common in cultivation.[](https://www.mdpi.com/2311-7524/9/7/807) - **Secondary Metabolites**: Glutamine and TMG may enhance cannabinoid and terpene production by supporting metabolic pathways (e.g., methylation, nitrogen metabolism). EAAs like methionine could influence sulfur-based terpenes. However, direct evidence for cannabinoid enhancement is sparse.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Nutrient Efficiency**: Glutamine and EAAs improve nitrogen and micronutrient uptake, while TMG may enhance nutrient transport, optimizing cannabis growth in nutrient-limited systems like hydroponics.[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) ### Potential Risks and Considerations - **Inhibition at High Doses**: High concentrations of proline or certain EAAs (e.g., lysine, valine) can inhibit growth if used as sole nitrogen sources. Balanced mixtures mitigate this, as glutamine can antagonize inhibitory effects of proline or other amino acids.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Nitrogen Overload**: Excessive EAAs or glutamine may lead to nitrogen toxicity, causing leaf burn or stunted growth in cannabis. Monitor plant response and maintain balanced nutrient solutions.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **TMG Metabolism**: TMG may increase trimethylamine production in the soil microbiome, potentially affecting cannabis root health if not managed. Healthy soil microflora can mitigate this.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **Application Timing**: Foliar sprays (150–500 mg/L for EAAs, glutamine, proline; 0.5–2 g/L for TMG) during vegetative growth or early flowering maximize benefits. Avoid overuse during late flowering to prevent nutrient imbalances affecting bud quality. ### Cannabis-Specific Insights - **Hydroponics and Soil**: In hydroponic systems, EAAs and glutamine ensure efficient nitrogen delivery, while proline and TMG support stress resistance in fluctuating pH or nutrient conditions. In soil, maintaining microflora is crucial, as it naturally supplies amino acids; supplementation should be moderate.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cultivar Variability**: Cannabis cultivars differ in nutrient demands and stress responses. Sativa-dominant strains may benefit more from proline and TMG for stress tolerance, while indica strains may respond better to EAAs and glutamine for dense bud growth. Testing is needed. - **Secondary Metabolites**: While amino acids influence secondary metabolism, direct effects on THC or CBD levels are unconfirmed. Glutamine and TMG may indirectly enhance terpene diversity via improved photosynthesis and methylation.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) ### Practical Recommendations "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨 - **Dosing**: Apply EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) via foliar spray or root drench twice weekly during vegetative growth and early flowering. - **Combination**: Use a balanced mix (e.g., 250 mg/L EAAs + 250 mg/L glutamine + 50 mg/L proline + 500 mg/L TMG) to avoid inhibition. Test on a small batch first.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Monitoring**: Check for signs of nitrogen toxicity (dark green leaves, clawing) or stress relief (improved vigor, greener leaves). Adjust doses based on plant response. "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to enhance uptake.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient lockout. In soil, ensure healthy microbial activity to complement amino acid supplementation.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### Evidence Gaps - **Specific Combination**: No studies directly address EAAs, proline, TMG, and glutamine together in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid Impact**: Limited data on how these compounds affect THC/CBD levels. More research is needed on secondary metabolite pathways.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **TMG in Cannabis**: TMG’s effects are extrapolated from other crops. Cannabis-specific trials are needed to confirm benefits. ### Conclusion Applying EAAs, proline, TMG, and glutamine to cannabis plants can enhance growth, stress tolerance, and nutrient uptake, with potential benefits for yield and secondary metabolite production. EAAs and glutamine drive biomass and nitrogen efficiency, proline and TMG bolster stress resilience, and their combination may optimize cannabis health in challenging environments. However, careful dosing and monitoring are crucial to avoid inhibition or toxicity. For best results, test small-scale applications and consult with a horticulturist familiar with cannabis cultivation. If you want more details on application methods, specific cultivars, or further searches for cannabis-related studies, let me know!""🚨🚨🚨🚨 🚨🚨 7/31 125am!!!!! Adding L-glycine to the combination of essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine can further influence the effects on cannabis plants, as glycine is a non-essential amino acid with unique roles in plant physiology. Below, I outline the potential effects of L-glycine alone and in combination with the previously mentioned compounds, focusing on their impact on cannabis growth, stress response, and secondary metabolite production. Since specific studies on this exact combination in cannabis are lacking, I’ll integrate findings from plant science and infer synergistic effects based on known mechanisms. I’ll keep it concise and tailored to cannabis cultivation. ### Effects of L-Glycine on Cannabis Plants L-Glycine is a simple amino acid involved in protein synthesis, stress response, and metabolic pathways: - **Growth Promotion**: Glycine (50–500 mg/L) enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and rice, likely benefiting cannabis vegetative growth. It serves as a nitrogen source and supports protein synthesis, complementing EAAs and glutamine. - **Stress Tolerance**: Glycine acts as a precursor to glutathione, a key antioxidant, reducing oxidative stress from drought, salinity, or high light. In cannabis, it may improve resilience in challenging environments (e.g., outdoor grows or high-intensity indoor setups). - **Secondary Metabolism**: Glycine is a precursor to purines and chlorophyll, potentially enhancing photosynthesis and energy metabolism. It may indirectly influence cannabinoid and terpene production by supporting metabolic pathways, though direct evidence in cannabis is limited. - **Nutrient Uptake**: Glycine chelates micronutrients (e.g., iron, zinc), improving their availability, which is critical for cannabis in hydroponic or nutrient-poor soil systems. **Application Notes**: Foliar sprays or root drenches (100–500 mg/L) are effective, often applied twice weekly during vegetative growth or early flowering. ### Synergistic Effects with EAAs, Proline, TMG, and Glutamine Adding L-glycine to the mix may enhance the effects on cannabis plants in the following ways: 1. **Enhanced Growth and Biomass**: - **Glycine + EAAs/Glutamine**: Glycine complements EAAs and glutamine in protein synthesis and nitrogen metabolism. Studies show glycine (250 mg/L) combined with glutamine enhances shoot and root growth in plants, likely amplifying cannabis vegetative vigor and bud development. - **Synergy**: Glycine’s role in chlorophyll synthesis boosts photosynthesis, supporting the energy demands of EAA-driven protein synthesis and glutamine’s nitrogen assimilation, potentially increasing leaf area and yield. 2. **Improved Stress Resilience**: - **Glycine + Proline/TMG**: Glycine and proline both contribute to glutathione production, enhancing antioxidant defenses. TMG’s osmoprotectant properties further stabilize cells under stress. Together, they may improve cannabis tolerance to drought, heat, or salinity, maintaining growth in adverse conditions. - **Cannabis-Specific**: This combination could be particularly beneficial for outdoor cannabis or indoor setups with inconsistent temperature/humidity, reducing leaf wilting or nutrient stress. 3. **Secondary Metabolite Production**: - **Glycine + Glutamine/TMG**: Glycine and glutamine are precursors to metabolic pathways (e.g., GABA, purines) that may influence cannabinoid and terpene synthesis. TMG’s methyl donation could enhance these pathways by supporting enzyme activity. While direct evidence is sparse, this stack may increase terpene diversity or cannabinoid yield in cannabis buds. - **EAAs Contribution**: Methionine (an EAA) and glycine may support sulfur-containing compounds, potentially enriching terpene profiles. 4. **Nutrient Efficiency**: - **Glycine + EAAs/Glutamine**: Glycine’s chelating properties enhance micronutrient uptake, complementing EAAs and glutamine’s role in nitrogen assimilation. This could optimize nutrient use in hydroponic cannabis systems, reducing nutrient lockout. - **TMG Synergy**: TMG’s role in cellular hydration may improve nutrient transport, amplifying glycine’s effects on micronutrient availability. ### Potential Risks and Considerations - **Inhibition at High Doses**: High glycine doses (1000 mg/L) may inhibit growth by disrupting nitrogen balance, especially if combined with EAAs or glutamine. Studies on Arabidopsis show glycine can be toxic as a sole nitrogen source, so balanced mixtures are crucial. - **Nitrogen Overload**: Adding glycine to EAAs and glutamine increases nitrogen input, risking toxicity (e.g., leaf burn, stunted growth) in cannabis. Monitor for dark green leaves or clawing and adjust doses. - **Competition for Absorption**: Glycine, EAAs, and glutamine may compete for root or foliar uptake, reducing efficacy. Split applications (e.g., glycine in the morning, EAAs/glutamine post-light cycle) can help. - **TMG Interaction**: Glycine and TMG both influence methylation pathways, but excessive methyl donors could disrupt metabolic balance in sensitive plants. Stick to moderate doses (e.g., 100–500 mg/L glycine, 0.5–1 g/L TMG). - **Cultivar Variability**: Cannabis strains (e.g., sativa vs. indica) differ in nutrient and stress responses. Glycine’s benefits may be more pronounced in stress-prone sativas or nutrient-sensitive hybrids. ### Practical Recommendations - **Dosing**: Combine glycine (100–500 mg/L), EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) in a balanced foliar spray or root drench. Apply twice weekly during vegetative growth and early flowering. - **Application**: Use foliar sprays for rapid uptake during vegetative stages or stress events. Root drenches suit hydroponic systems. Test on a small batch to assess plant response. - **Monitoring**: Watch for nitrogen toxicity (dark, curled leaves) or stress relief (greener, more vigorous plants). Adjust concentrations based on cultivar and growth stage. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to maximize glycine’s chelating benefits. Ensure adequate magnesium for chlorophyll synthesis. - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient imbalances. In soil, maintain healthy microbial activity, as microbes naturally produce glycine and other amino acids. ### Evidence Gaps - **Specific Combination**: No studies address glycine combined with EAAs, proline, TMG, and glutamine in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid/Terpene Impact**: While glycine may support secondary metabolism, direct effects on THC/CBD or terpene levels are unconfirmed. Cannabis-specific trials are needed. - **TMG and Glycine**: Their combined effects on methylation in cannabis are speculative, as most TMG research focuses on other crops. ### Conclusion Adding L-glycine to EAAs, proline, TMG, and glutamine likely enhances cannabis growth, stress tolerance, and nutrient efficiency by supporting protein synthesis, antioxidant production, and micronutrient uptake. The combination may improve vegetative vigor, bud quality, and resilience to environmental stressors, with potential (but unconfirmed) benefits for cannabinoid and terpene production. Careful dosing and monitoring are essential to avoid nitrogen toxicity or absorption competition. Start with low doses, test on a small scale, and consult a cannabis cultivation expert for tailored advice. If you want me to search for more studies, focus on a specific aspect (e.g., glycine’s role in terpene synthesis), or provide application protocols for a particular cannabis strain, let me know!"""

Week 2 feeding wasn’t much higher than the 1st feeding. Starting to see growth starting to explode.

Started removing leaves that were dying. Had to tie one plant down she grew bigger then expected and was getting to close to the light