Eerste paar dagen in een kas. Over 3 dagen rond 25 graden dan zet ik ze buiten de kas. Heetste uren zet ik ze beetje beschut mocht het boven 24 graden komen

As you can tell getting more top heavy buds definitely filling in the smell definitely picked up trying to push to day 70 I don't have a microscope yet but if somebody can tell through the pictures if the time may be sooner than 70 day please speak your mind

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Hi everyone :-) This week it has developed really well 😍. it was also topped for the first time. Like the Cup, I will not do an LST here either, just top it a few times 😃. I wish you all a nice week, stay healthy 🙏🏻 and let it grow 🌱

They were both fed today with 1.15 liters of approximately 6.10 ph balanced water with the following nutrients: Growth science, big bud and extra cal mag! They have all shown signs of recovery with new growth sites having no signs of cal mag issues or any other deficiencies. Gorilla cookies did amazing with topping, her neighbor not so much and I will definitely not top the Afghan kush from fast buds next time. New gravity fed watering system installed today! Until next time!

Autopot system was turned on day 15 !! Now everything is on automatic 🦍🍓🦍🍓 Im not touching this girl until day 50 or some like that, no LST, no topping, just a stress free VEG, lets go ! NO DEFOLIATION AT ALL

Day 32 - 12/01/20 Everythings going fine for the Pineapple Express 🍍 I swapped out the bungee cords holding my grow light for rope rachets today. it wasnt hanging level so i still needed to use the bungee cords but the light is much higher now to accommodate the height of this monster! current height is 46cm which is about 18 inches. Im feeding with my normal Canna and Vitalink schedule and watering with just phed water a lot too. they drink so much when in the proper temperature range and healthy! Nothing much to report other than that although i think im gonna invest in some more bud boosters and terpene boosters as im struggling to get a huge flavour from my buds. its noticable and its there after curing, each strain i have grown has its own unique flavour and smell but it just hasnt been potent enough for me yet. possibly due to my previous strain choices. anyways thats why im growing more fruity and higher thc strains. also for the medical benefit, the 1:1 THC/CBD weed was good as a supplemental medical weed i found that i still needed a high thc strain as a base to stay pain free and stoned. and the lower thc varieties ive tried are nice but just arent strong enough for a heavy smoker like me to relieve pain and help me sleep. Il update later in the week, and apologies again for not updating the diaries as much ad i usually do, ive been quite busy over the new year! Happy growing folks!

I didn't bother updating the diary last week as there was nothing going on really, just finished the 1st week of flushing and another week till harvest. Only big change going on is the hps and little viparspectra LEDs have been taken out and replaced by 2x invisible sun ISH265 quantum boards.

You walk into the back yard and it’s a straight face punch of smells. Looking like some indoor rn moved all plants to safer place in case storm hits so now I atleast have a wind break

Flowering is really stunned in this very cold weather, still a fun thought seeing my plant still alive while all the tree already have lost their leaves. Today i chopped the remaining branches on my right maincola, the left will stay 1 to 3 more weeks, im gonna push that one with all the risk of every risk🤗 i wett trimmed the branches again and hanged them, after i took away the first drying batch from 10 days ago. I weighted that what was still hanging at 49 grams dry weight, adding up to a total of 64 grams so far... Im guessing the total amount of dry weight on this plant will be around 300 grams unfortunately... If this would have flowered lets say 1,5 month earlier( like it was fking expecter to do!!!) It would have made a kilo for sure

🛁 Beginning to clear nutrients. ✌️🎃 Thank you for checking my cultivation.

This is the whole flowering cyclus posted in one week.

Heute ist tag 3 in meiner box. Wurzeln alle super an und suchten ab minute 1 das licht. Schöne weiße wurzeln und saubere pflanzen👌💚 Bin schon gespannt auf diese ladys. Die besten und saubersten stecklinge gibts wie immer bei ROOTS-FARMS. The best in austria and maybe europe🔥👌

She's clearly not happy. She hasn't been happy for a couple of weeks looking back at past weeks.

Estamos en las ultimas. Se ve que ya no quedan mas de unos 10 días (a la purple quizás un poco mas) pero dentro de todo ya esta finalizando el resultado. No es el mejor de todos, mucha asimetría, mucho problema y mucha irregularidad en el cultivo que dentro de todo hacia complicado saber de dónde pudo haberse generado el problema, pero aun asi algo de producción obtuvimos. Más allá de revisar la productividad de este cultivo, veremos qué tal la calidad de este.

Decidí no hacer ningún corte Tampoco ningun lst ni nada Solo dejarlas ser por esta vez...

Noch 1-2 Wochen