On day 15, I defoliated the girls. The girls are growing up like crazy, they have already doubled their height. Everything is going well, I'll probably have to do supercroping in the following days ...

This plant was RIP ed out of the ground and put inside looks bit fucked but shes smell so nice I ripped a bud off to smoke and boy is she strong

All content on this diary is for inspirational and educational purposes only. The ideas shared are not a substitute for professional advice. This diary/account is not officially affiliated with Alan Watts or his estate. All materials are used under the principles of fair use. I honor the legacy of Alan Watts by sharing his wisdom respectfully and with the intention of inspiring awareness and self-understanding. 2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Bestva Quantum Board / Samsung LM301B Mr Hide Seeds Semana de engorde e inicio de lavado de raíces para dos de ellas. De momento todo se ve en orden, buen olor y aspecto pesado. Buenos humos a todos !

4. Woche Das Topping ist soweit abgeschlossen, 3 Topps sind es am Ende geworden und jetzt darf sie sich erholen und ihre Kraft um modellieren 😊

I’m very impressed by this strain. This is my first Atlas Seeds grow and it’s dominating my Mephisto genetics in frostiness. She’s already browning on the pistils, this will be a fast, potent, high yielding harvest!

Great week 4 for my 2 lovely Papaya Sherbet

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Hey there, As I went to the Mary Jane some weeks ago, I got a Promo Seed from Wizard Trees. I‘m usually not the Cali-Weed-Guy, because I don‘t like the taste and the colours… it‘s always so dark and I personally like „light green“ weed… you know, oldschool. Just good weed and not some hokus pokus. PPFD ~ 160 Let‘s see what this turns out. I will definetly keep it as a mother plant and take clones. See you around… ✌️

Watering less at a time but more often now. Spent some time twisting the main stem to assist in bending her over. Secured down between the first and second node of 3 currently. Stem is very rigid so I moved some soil around the base to relieve some stress on the base. LST seems to be doing great since I was forced to move the tie downs from the main stem to the fan leaves. Watering with Real Growers Recharge on 5/2/22.

~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_ 08/07/21: 😸Week 4!! (a day early, Sunday plans etc..).. We've been feeding lightly with every watering (we're in promix) but we may throw some EWC in their pots..the mainlined plant is sporting new growth and building symmetrically, she had almost no recovery downtime to speak of, i couldn't be happier 😻..the low stressed plants are starting to fill out, we've been doing a lot of leaf tucking with those 2. thanks for reading if you made it this far lol, we'll update midweek..happy growing folks! 😽💡🌱❤️ ~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_ 8/10/21: 🐱 The new growth seems to be coming light colored, they're definitely getting enough to eat for their size..idk..ill just keep doing the same routine and make changes if/when it gets worse..ty for stopping by ❤️💡🌱 ~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_~_

unreal 👌🏻👌🏻👌🏻 despite the high temperatures, it's incredible❤️

Just feeding it at the moment. Still not to sure if it's male or female yet. She is female 😁.

Day 36: Girls looking pretty happy. Still some discoloration going on. Doesn't look to be spreading though. Day 37: Ok, the girls obviously were not happy this morning. I watered with quite a good amount of runoff and measure the PPM at 3200!!!!!!!!!!!!!!!!!!!!! I'm surprised they are still alive! Had to make a quick run to get some water for a flush as I only have 5 gallons worth. Ran 2 gallons through each of them at a ph of 6.4 around 9:30 this morning. It's now 5pm and they have pulled through great. These OG Kush are extremely resilient. Day 38: The girls look way better today after flushing yesterday. Two of the pots were almost completely dry already. Went ahead and fed at 500ppm with runoff. Day 39: I think it's dialed in now. They are looking amazing and still stretching pretty good. Went ahead and pulled off a handful of leaves between the 5 of em. Day 40: Did a little more defoliation and opened them up a bit. I am super worried about getting any type of mold or any issues in particular for this first round. One of the girls needs a touch more but ran out of time. Girls are looking super healthy! 💪 Day 41: They are looking super awesome. Got the runoff dialed in. 750 going in and roughly 800 coming out. May up it in a week or so. Day 42: Nothing new to update. Girls still looking amazing. I think the stretch may be slowing just a tad.

They go fatter day by day, still clear trics, and some nuggs are littel under developped further down stem, but massive colas covering the whole surface, smells like fruity candy and sticky as hell, resun production is insane. I just dont eant to get mold drying this, any yips? Whole plant or chop of piezes and hang or dry net, colas are real fat and rock hard.. counted round 35 top colas

Day 30. Girls will get heavy feed in a sec. They recovered well from mini lolitoping session. Made mini upgrade in Grow room. All 3 quantum boards are now on one platform. Plus i ordered 2 month ago led strips from China, so even with 3M tape ( what i don't believe was original 3M ) they just didn't glue properly and after 2 days fell off. Plus its not even close to grow light, no power at all. Guess its my most stupidly spent 15 quid in all grow career. Wanted some mini red boost for flower, but ill just take them down. Girls grow really nice, some are stretched , some are a bit dwarfy. But in general all good, no female or hermie signs tho. Left 3 or 4 nods at that time, all lower part was cleaned with razor.. Didnt use any prevention, but girls healed well. Think flower not far away too.... Day 30 . EDIT : Girls are showing sex, so flowering started ? !!!! ;) Day 32. Girls loved heavy feed, all stretching and flowering. Some strains are obviously smaller than others, will be fun to guess what is what ;))) Can't wait to give all tent to them, autos are on the end stretch. 3 more weeks max and all tent will be pure bag seed go ;) Happy Growing !

Не занимался растихой из-за "перезагрузки" своей оранжереи. + Тестирую видео-формат, пока первая "сырая" наработка. Cali #CannapediaGrowersCup4th мощное растение, легко пережила все стрессы, что подкинула ей судьба. Несмотря на то, что 5 дней стояла под комнатным светом, и лежала в коробке — быстро оклемалась и даже практически не растянулась от недостатка освещения, лишь чуть нарастила ветви. В ближайшие пару дней соберу ей бокс и флипну на цвет. G.H.Feeding Grow + pH Down, с показателями 6.2-6.5pH и 700-750ppm, раз в 2-3 дня.

its day 35 since sproud and the plant looks good so far. I removed some leaves and started training her with that bending clips to make the branches stronger. I collected leave mold from the surrounding woods. a lot of white fungy hopefully usefull mycoriza.