Lowered lights to 50% and raised them to 26 in.

My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems.

6/6/2023 Week 9- Day 1 of Flower (Day 124 overall) (Day 63 of Flower) Auto Pilot Day 3.. Just watched them be beautiful. I also did a Trichome check today things are moving along nicely. 6/7/2023 Week 9- Day 2 of Flower (Day 125 overall) I admit it I couldn't help it there were some blatant offender leaves blocking the light from some of the canopy buds so they had to go..... and when I took them I found a few more in between leaves that had to go.. so long story short some defoliation did happen.. :-) 6/8/2023 Week 9- Day 3 of Flower (Day 126 overall) Auto pilot re-engaged.. I sat back took some photos and just enjoyed how beautiful this light side and dark side thing the plants are doing The buds are almost as long as my Sharpie. I also applied the last application of my Hermie spray to the clone I took in Apr to reverse it. 6/9/2023 Week 9- Day 4 of Flower (Day 127 overall) Auto Pilot Day 2... :-) It was check in day today for the grow off. Last check in for the competition. Trichomes are looking great.. did another check today will keep an eye on it just incase I need to cut earlier than planned. Tomorrow Water change day! 6/10/2023 Week 9- Day 5 of Flower (Day 128 overall) Auto pilot for the Ladies' but Water Change Day! I added 36 gallons Mammoth Silica = .5Mil/Gal= 18Mil Root DRIP = 1Mil/Gal= 36Mil CalMag= .5Mil/Gal= 18Mil FloraMicro = 3.8Mil/Gal= 137Mil FloraGro= 2.8Mil/Gal= 100Mil FLoraBloom= 4.3Mil/Gal= 155Mil LiquidKoolBloom= 2.5Mil/Gal= 90Mil TPS SIGNAL= 3Mil/Gal= 108Mil ORCA = .5Mil/Gal= 18Mil Water Temp= 71.2 Tent Temp= 76 6/11/2023 Week 9- Day 6 of Flower (Day 129 overall) Auto Pilot Day 4... I just enjoyed the nice smell coming from the tent today, and the contrast between light and dark side buds that they did. 6/12/2023 Week 9- Day 7 of Flower (Day 130 overall) Auto Pilot Day 5. These ladies are looking amazing, the fade is coming in nice purple on plant #3 and really nice yellow on #2.

Wizard Fuel - Wizard Trees Day 84 - Watered with 3L of RO tap water with nutrients mixed individually, in order and then pH'd to 6.3! Week 7 of bloom and shes lookin' and smellin' so good, I'm super excited to try this one! Her buds are big heavy, rock solid, colourful, vibrant and covered in trichomes! She has a great structure and all her main budsites are getting the light they need to bulk up 💪 I have started to remove the larger fan leaves to allow more light onto her surface, as I want her to get the most out of this week, because from next week I will need to start preparing her for harvest 👌

Overall I enjoyed this grow and learned a lot about living soil. Going for a slow dry, trim, then at least a one month cure. Hopefully another six days and I’ll update with a dry yield. My next batch is already cooking but with 5 gallon pots. 03/25 - Day 7 Drying B & C are done drying and have been in jars for a day now with RH ~60%. Total 3oz from B & C. Total 1.5oz from A & D

Riego: 1ml Bio grow 1ml Top max 1ml top bloom 1ml calmag 1ml Bio Heaven 0.5 Micorrizas colectivo científico Se han ido ajustando los amarres de LST cada día por medio aprox.

Flowering day 12 since time change to 12/12 h. Hey everyone ☺️. Once again a wonderful week goes by 😀. It has developed so far and is now slowly starting to develop its flowers :-). As soon as the first forms of buds can be seen (I think in 5-10 days) 2 g GHSC Powder Feeding Bio Bloom per liter of substrate are added. In the third week, 1 g per liter of substrate is added so that it lasts through to the end :-). This week it was poured 3 times with 1 liter each 👍. The entire tent was also cleaned and the electronics checked for functionality. The humidifier was refilled every day and fresh osmosis water was drained into the tank for the coming week ☺️. The plants have all been checked for health and pests 👍. Next week the bamboo sticks are inserted towards the end for support and the lowest shoots are removed so that at the end the energy is not wasted in the lower area 👍. Otherwise there is nothing more to report this week :-). I wish you a lot of fun with the update 😎. Stay healthy and let it grow 🍀. You can buy this Strain at https://www.amsterdamgenetics.com/product/kosher-tangie-kush/ Type: Kosher Tangie Kush ☝️🏼 Genetics: Kosher Kush X Tangie 👍 Vega lamp: 2 x Todogrow Led Quantum Board 100 W 💡 Bloom Lamp : 2 x Todogrow Led Cxb 3590 COB 3500 K 205W 💡💡☝️🏼 Soil : Canna Coco Professional + ☝️🏼 Fertilizer: Green House Powder Feeding ☝️🏼🌱 Water: Osmosis water mixed with normal water (24 hours stale that the chlorine evaporates) to 0.2 EC. Add Cal / Mag to 0.4 Ec Ph with Organic Ph - to 5.5 - 5.8 .

Hi everyone 🤗. Everything is going great 👍. There are no defects or anything unusual :-). Today the Blue Cheese Pheno 1 became the first time rinsed, and now only gets 0.4 Ec water for the next 10 days ca :-). Everyone else needs a while. otherwise there is not much to report this week. I wish you all a nice week, stay healthy 🙏🏻 and let it grow 🌱

Siamo arrivati alla 10 settimana,una pianta ha incominciato il flush,quella al centro di destra.i fiori delle piante più avanzate sono grassissimi e ricchi di resina,questa pianta é davvero ECCEZIONALE,daremo qualche altra settimana di acqua e raccoglieremo la prima!ricordiamolo sono tutte autofiorenti.GORILLA COOKIES AUTO

I visited her today!!!! Day 147!!! when I came I saw one broken branch, yesterday there was quite a strong wind, I think it's his fault :) but everything else looks good, just found 1 small ring of rot :), which allows me to leave it to grow for another week :) very beautiful girl :) there will be a renewal, good luck!!!!

4.14.25 2nd week of veg! Started tie down method on 4.7.25 going good. Nice healthy growth! Going to start wrapping plant around perimeter of pot.

More vertical growth again this week. Another 10cm higher! Got even bushier again too she’s a beast. Buds r growing nicely too so no complaints really. I struggled big time trying to keep humidity levels down to normal amount. Was even reaching 80% at times so I bit the bullet n bought a mini dehumidifier for the space. It’s doing the job so happy days

Harvesting An ELEPHANT !!! And after 118 day from seed and 101 from wen she started her mix veg/flowering process, i cant say for sure but i am starting to believe that this auto world do veg, then veg and flower in conjunction for a wile and only after they a wile they move to a flowering stage completely but thats another post another day lol today was harvest day for this Auto Elephant from SSSC , and let me tel you she is a delight with huge fruits, so big that i normally cut and hang the all plant and for this i did not take the risk of doing that and getting bud rot , better safe than sorry, so what i broke her in smaller peaces and put it all in the rack. Now not much to do than waiting for it to get around 10/15% humidity and jar with it all. This one as you guys can see from the video , this is a full covered in trichomes BIG MAMA and touching her is like touching super glue or something lol i was trying to put all the colas in one picture , but is simply just not possible , they all look great so i selected 4 of them for a true back back ground <3 Thank you Super Sativa Seed Club for this opportunity, i fell like a champion no matter what with this 2 Mamas, much respect on the work you guys did on this Elephant As always thank you all for stoping by for your love and for it all, i fell humble and honored

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Inizio fioritura.prima settimana bene bene bene.. continuate così 💪✌️🙏 Una delle 3 papaia è diventata Maschio.peccato.....

June 13: cool and rainy spring but she will start growing quickly with warmer temperatures near the solstice. This backup seed is actually about 5 weeks old and not seven. June 14: nice weather today but supposed to get rainy again this week. June 17: did first topping for the manifold. Rain again today. June 18: more rain again today.

Week 10 for GMO auto by atlasseed Both the indoor & outdoor auto (outdoor was mostly indoor expect last 2/3 weeks i think?) Anyway they are both drying, my wife & I had a tester smoke of the outdoor plant & both had uncontrollable laughing fits.. genuine laugher it was a refreshing high yet my face hurt after😂 The indoor smaller plant definitely crushed it in the looks department way chunkier, the way the outdoor one finished in 42+ sun probably has a lot to do with the looks deference. Will update on smoke report etc when everything is dried. Lets find out if she's getting growth again or not😇.