Hey everyone :-) This week it smells more and more sweet and fruity in the whole room 😍. There is actually not much to report, everyone is now under 12/12 :-). This is the last grow that comes in the entire diary. From now on, each plant will be added to the diary 👍. I wish you all a lot of fun watching, stay healthy 🙏🏻 and let it grow 😎👌

Wow isn't she looking fab this week?? 😍 Flowers are really plumping up. 😎 Few more weeks yet I think.

Just a little defoliation this week, not much else. The main cola is getting nice and big and it’s very dense. The rest of the coals are not very big but this plant has got little buds growing all over the place. I think this plant still has a couple of weeks to go but will have to wait and see, the pistils are still pretty white. This is my first time growing so i don’t have anything to compare this small size to, might be genetics or it might be that I stunted this early on by not watering from the bottom or wrong water ph or what have you.

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

This past week has been a blast! After getting these ladies nutrition straightened out, they are thriving!💚🌱💚 I gave defoliated wayyy more than what I see other growers traditionally doing. After the "3rd and final haircut" I defoliated two more times 😅 Thank you the GD community for the support and resources along the way 🙏 My only concern now is the tent actually becoming too crowded.😆. Other then that I am thinking it should be smooth sailing these next 6 weeks or so. 🏴‍☠️😎🏴‍☠️ Happy Growing! 💚💪🌱😎🌱💪💚

The germinated seeds were planted with the white tap-root pointing downwards into small pots (0,25 l) with light-mix from Goldlabel.nl. During week 1 almost all seeds came up and grew into small seedlings. Some late-comers like the MAC are just peaking out of the soil, while others are already small plants of 5-7 cm size. During this phase I only use one single S4W-LED lamp from SANlight in Austria. The fixture is appr. 50 cm above the babies and this is plenty of light for the small seedlings. They get a little bit of BIO NOVA roots with their water, because this enhances root growth a lot and helps the babies to stay healthy until they are tall enough to be repotted. Don't let the pics with the KANNABIA strain-markers in the small pots confuse you, the genetics are all different strains from different producers (see germination)! Three strains are from KANNABIA SEEDS (the Kritikal-K, the Pineapple Dream and the Skit Kandy Dream), but they came with a bunch of handy plastic strain markers, which I used for all my plants/pots.

🌿 Week 10: The Final Countdown & The Autumn Fade 🍂 The Vibe: We’ve officially entered double digits! The tent is a jungle of smells, and the air is thick with a mix of sweet pineapple, heavy cream, and that funky cheese undertone. Most of the white pistils have now tucked in or turned orange/brown, and the plants are starting to pull mobile nutrients from the larger fan leaves the "autumn fade" is officially here! The Ladies: Pineapple Express F1: Still the undisputed Queen 👑. Her main colas are rock hard and coated in a sticky layer of resin. She’s starting to show some beautiful purple/yellow hues in her fan leaves as she nears the finish line. White Runtz: The "Chonk" of the tent! 🍩 Even though she the tallest girl is the tent, her bud density is incredible. The frost on the Runtz is next level it looks like she’s been dipped in sugar.(And she is now drying) Blue Cheese F1: My little rebel. 🧀 She stayed compact and "boujee" with her 3-fingered leaves until the end, but don't let her size fool you the smell coming off her is the most pungent in the tent. She’s finally stopped that weird side-branch stretching and is putting all her energy into ripening those dense little nuggets. Care & Nutrients: Feeding: This is the last week of full nutrients. I’m still using the Bio-Bloom and Top-Max combo, but I’ve started tapering down the Fish-Mix and TNT Complex to let the plants use up their stored nitrogen. Watering: They are thirsty! I’m still giving about 1L per plant every other day, ensuring the pH stays steady at 5.5-6.0 to avoid any late-stage lockouts. Environment: Keeping the humidity lower (around 45-50%) now to prevent any bud rot issues since the flowers are so dense. Temperatures are steady at 27°C lights on. Next Steps: It’s all about the jeweler's loupe now. I’m checking trichomes daily. We are looking for that perfect 10-20% amber ratio before we start the final flush. It won’t be long now! Question for the Growmies: Do you prefer to harvest when trichomes are all cloudy for a "heady" high, or do you wait for more amber for that "couch-lock" feel? Let me know below! 👇

La altura de las plantas ronda entre los 45 y 60cm. Durante esta semana se realizara una poda, todavia no realizada al momento de hacer este seguimiento. Se prosigue el riego 3 veces por semana, lunes, miercoles y sabados. Aparecen los primeros pistilos que dan comienzo a la flora.

She looks very super big and beautiful for her 3rd week since planted, let's see how she keeps developing, looks super happy.

Bonjour à tous les padawans et maîtres jedis Je me régale ! Franchement quel plaisir toute les conditions sont réunis pour faire une belle culture et le résultat est très intéressant Un stretch presque fini est une belle plante avec un beau 108 centimètres Un arrosage avec 2 litres d'eau ph6.3 à laquelle j'ajoute 5 millilitres de Hesi bloom et la dose recommandée de supervit

Meanwhile, our flowering cycle continues. In just one week, the plants have changed noticeably and are developing exactly as expected.

In to week 7 now starting to flush!! Very impressed with this run 💚🌱💚🌱 I’m now running the Futur vert 30 W uv led for 5 hours of the 12/12 with the Futur vert flora max series

I was doing LST, and while bending the leg, I broke it at the very stem. supported with a small bamboo so that the fracture touches, is there any chance of loss?

Day 22 22/07/24 Monday Nothing new to report. Starting to see them beautiful fans appear that's all 😁✌️ Day 23 23/07/24 Tuesday Feed today, giving them straight all nutes Inc flowering nutes. I will update the dose as they grow and develop a bigger hunger. (Not sure what's wrong with Overdose #2, bit mutated, we'll see how she gets on 😅) Video updates 👌💚 Day 26 26/07/24 Friday De-chlorinated watering pH 6 with 5ml calmag to 5L water. Watered each with 250ml and had small run off. I will continue to feed/water with small run offs to help prevent salt build ups. Video update.

Day 37: The girls are growing fast and strong! They also seems to have turned into flowering state now. Everything seems good. Ive been recommended to just let the girls do their job from now, and I won't touch them for awhile, but I think I soon will give them some more nutrients as they are now in flowering state.

!!!!elle a tout pour plaire jusqu’à présent.

4. Woche Alle Ladys wurden 3x getoppt, jetzt mache ich eigentlich nicht mehr viel, nur noch bisschen in Form bringen und dann dürfen die Ladys wachsen, wie sie wollen 😁

Alright! I'm updating the journal a couple days early bc my birthday is this weekend!😁 Ms. Hypnotic is growing beautifully! Shes a little leggy so I'm going to create a bean pole out of a toothpick as I've done before to give her additional stability! I've still been feeding roughly around every 2 days but she really doesn't need much at all. Next week I will take her into bloom like he sisters if she holds steady this week! Please drop likes, comments, constructive criticism, encouragement and or Happy Birthday wishes! Happy growing!