Week 8 - we are getting so close. The bud smells so amazing. It’s a citrus earthy smell that I can’t wait to taste. The weather is cooling down and she seems to like that. I wish I had more hours of light, but she’s as big as she is going to get.

! this is my current status, gonna upload my last veg weeks within the next week - got a bit busy around Spannabis - thank you for your understanding! 💚 Welcome to Bud Boutique Grow Diary - really appreciate all your love and support :) Dont forget to check out my other current grows! 🗓️ This Week: after 48 hours of complete darkness (important for seedlings to make sure all plants switch into flower same time), I finally switched my lights to the 12/12 schedule and sent my ladies into the flower. - This one Phenos good in bud sights production but still cant catch up with the others, so I raised to pot by 10-20cm depends how it will move on - bud besides this more than healthy and going well - Day 3: Foliar spray with APTUS Regulator and APTUS Nutrispray to give them extra micronutrients and give them a little shell for protection. ! always use APTUS Regulator first and mix well with your water before adding any other nutrients for Feeding or Foliar! Thank you for still staying with me 💚 ___________________________________________ --- 🌱 Strain (Sponsor) 🌱 --- 🏷️ Big Band by Kannabia Seed Company https://www.kannabia.com/en/feminized-cannabis-seeds/big-band --- 🥗 Nutrients and Feeding (sponsored by APTUS: APTUS Ambassador) --- 🍸 APTUS: full nutrient schedule extreme -- Regulator, N-Boost, P-Boost, CaMg-Boost, K-Boost, Allin1 Liquid, Startbooster, Topbooster, Enzym+ every feeding -- Fulvic-Blast, NutriSpray as Foliar each once a week 🔗 https://aptus-holland.com/ --- ♻️ Grow Control (Sponsor) --- TROLMASTER: TENT-X + LM14 Light Adapter to dim/sunrise/sunset lights + Temp & rH Sensor all remote on App 🔗 https://www.trolmaster.eu/ --- 🏭 Grow Setup --- 💡LUMATEK Zeus Pro 600 * 🏠🌿 Indoor: Homebox 120x120x200cm (4x4) * 📐🌀 PrimaKlima exhausting Fan 1180m3/h (running on 60-80%) * 🌀 Can Light Filter 800m3/h & 1x Fanbox 1x Dyson fan for Air circulation 🔗 https://lumatek-lighting.com/zeus-600w-pro-29/ 🔗 https://primaklima.com/de/shop/ventilatoren-de/ec-ventilatoren/pk160ec-tc/ 🔗 https://canfilters.com/products/filters/ All Likes and comments are highly appreciated!!! 👨‍🌾 don't forget to check out my Instagram for daily educational content: budboutiquee - Bud Boutique

Grow Diary Update: Week 5 - Pre-Flowering and LST Evaluation Entering Pre-Flowering Stage At the end of last week, the first pistils appeared, marking the beginning of the pre-flowering stage for my plants 🙌 There's much to manage now. To support optimal stretching, I've adjusted the nutrients following 2/3 of the Terra Aquatica feeding schedule: CalMag: 2 ml/l (Starting EC before adding nutrients: 0.45 mS/cm) Grow: 1.33 ml/l Bloom: 1.0 ml/l Micro: 1.33 ml/l The nutrient solution now has an EC of 1.3 mS/cm (638 ppm PPM-500/TDS) with a pH of 6.1. These adjustments aim to promote vigorous growth and prepare the plants for flowering. Weather and Growth With significant weather improvements, my plants are thriving at full speed. Every centimeter gained now counts towards maximizing the final yield, turning around from the challenging weeks prior. Pest Management I'm fortunate not to have encountered pests like spider mites, aphids, or thrips. However, the persistent snail issue remains. Nightly patrols with a flashlight have been effective, and placing old lettuce leaves away from the plants has helped trap and remove snails, ensuring the plants remain undisturbed. LST Experiment Results The two plants I trained with LST have responded admirably, showing healthy adaptation to the bending and vigorous growth. Yet, I'm at a crossroads regarding the upcoming stretch phase: should I continue to tie down branches to maintain an even canopy or allow natural upward growth to potentially boost vertical yield? Your Feedback Matters I value your insights on this critical decision. Should I proceed with low-stress training to control canopy height, or should I let the plants stretch upwards for increased productivity? Your experiences and advice are invaluable to me as I navigate this stage of my grow. Share your thoughts below! Stay tuned as I continue to document the pre-flowering stage and learn from the ongoing LST experiment in my grow diary!

Aquí seguimos, separamos ya los machos y nos quedamos con 5 hembras de las 9 que seleccionamos. Más del 50% muy contento. Durante estas 2 semanas hemos estado luchando contra una pedazo de plaga de mosquitos que hemos sufrido. La cosa parece que mejora pero habrá que estar muy atentos. La floracion va en camino podría ser mucho mejor pero la plaga ha hecho sufrir a las plantas. Iremos actualizando. Buenos humos.

Letzte Woche letzte LST und eine letzte EC 2,3 vegi calmag Lösung dann die Blüte in 7 Tagen max vielleicht in 4

9/19 - I did the thing y'all!! Both plants chopped at day 77! These are the chonkiest things I've ever had the pleasure of growing/handling. Time will tell how much they shrink in the dry. The plants are all drying in my dark tent and trying to get close to 60F/60% RH, but really closer to 65F/60% RH, so I don't freeze in my home lol Put up one more vid before I chopped. I still need to take a pic of TA roots, which are pristine!!! If you see the DG root pic, you can tell how hard it got stunted by the vacation fiasco. Can't wait to make some rosin and trim these up! Slightly nervous of drying based on the density, so I cut more dry leaves than usual, but I don't want to risk mold. Next update will be the official harvest post!! ---- Thanks for following 🤙 Always appreciative of tips or suggestions

Going to pull her out of the light tonight and put her into darkness. Very happy with the way she turned out!!

Hello. This is the end of week 6 and the beginning of week 7 of flowering. This plant shares the tent with lemon mandarin from fast buds and I found a spent male flower on one of the lemon mandarin plants leaves. So there is about 4 weeks left on the grow and I'm hoping that doesn't lead to a lot of hermie seeds. I'll have to look a bit harder and see what's going on in there. Made some kief out of the MAC and Crack I harvested in the fall. There were 3 light and fluffy sativa like plants that I'm turning to kief. Got a good amount of kief out of it and it's got a good head high too. OK. Keep Growing Straight. Chuck.

Topped and transplanted to final 9l airpot looks a bit tired but was over watering but now she is dry and happy

Ok guys had a bad week this week plants have been really affected went away took my eye of ball for 5 days plants ph has fucked up and caused alot of damage to leaves etc checked ph run off @6.4 so going to flush with 5.8 to hopefully get these babies back to normal also maybe a ec problem think may have been feeding too much I am a newby learning has a go so any advice help etc feel free to comment

Dats da light Ive been using, last time there were 3 lights on it' now just one. Snowy is doing just fine' looks like she made it now' She gonna show up and blow up When she gets mature

Day 42 Flowering: Hi all. Here we are past the halfway point and close to seeing some finished Fast Buds nugs. They have been starting to brown off the 1st pistols on most of the colas but the Six Shooter both look like they will be finished 1st. With the different growing styles used on them leading to different structures, it will be interesting to see which yields the most. The #2 has lots of nice forming shaggy looking tops and the #1 has more natural long colas. The Mexican airline are similar to each other but # 1 is less advanced into flower I think. They have browning pistils but the # 2 is way chunkier and.developed . What can I sat about rhe Strawberry pie that the video doesn't show ?. Wow this strain is a nice grower. The buds are nice and large with a fresh appeal to them even as they ripen. They are a 9 week (63 days) flowering strain so with so long to keep fattening and developing , I can only imagine their bounty of colas to come very soon. Well done fast buds The fc3000 is doing a brilliant job and hasn't skipped a beat so far. The canopy has been so uneven and hard to please the needs usually but this has not held anything back now. The penetration is excellent and has helped develop some amazing buds not in direct light too. Roll on harvesting these amazing plants. Be safe and well growmies

Not much change. Seeds take time. If I had not pollinated these plants I'd probably go ahead and harvest this. I included a video. Suggestions are welcome. I can't shoot without the grow light so sorry for that.

Day51: flowers are getting bigger, leaves tips are still under control. The environment is getting hotter and hotter due to the heatwave but the plants are holding on. Day55: i was not home for 3 days but the plants were fine, just thirsty, hot and with some gnats due to being closed for so long.

Info: Unfortunately, I had to find out that my account is used for fake pages in social media. I am only active here on growdiaries. I am not on facebook instagram twitter etc All accounts except this one are fake. Flowering day 29 since time change to 12/12 h. Hey guys :-) Once again a nice week has passed in which the lady has developed super :-). The buds start to grow and are now getting more pk. This week it was poured 3 times with 1.2 l each (nutrients see table above). Fresh osmosis water was filled in canisters for the next 2 weeks. Everything was cleaned and the plant was checked for health :-). I wish you a lot of fun with the update and stay healthy 👍 You can buy this Nutrients at : https://greenbuzzliquids.com/en/shop/ With the discount code: Made_in_Germany you get a discount of 15% on all products from an order value of 100 euros. You can buy this Strain at : https://greenhouseseeds.nl/ ☝️🏼☝️🏼☝️🏼☝️🏼☝️🏼☝️🏼☝️🏼☝️🏼☝️🏼☝️🏼☝️🏼☝️🏼 Green House Seeds Company Cup Clone 🏆 Type: Wonder Pie ☝️🏼 Genetics: Wedding Cake x OG Kush 👍😍 Vega lamp: 2 x Todogrow Led Quantum Board 100 W 💡 Flower Lamp : 2 x Todogrow Led Cxb 3590 COB 3500 K 205 W 💡💡☝️🏼 Earth: Canna Coco Professional + ☝️🏼 Nutrients : Green Buzz Liquids : Organic Grow Liquid Organic Bloom Liquid Organic more PK More Roots Fast Buds Humic Acid Plus Growzyme Big Fruits Clean Fruits Cal / Mag Organic Ph - Pulver ☝️🏼🌱 Water: Osmosis water mixed with normal water (24 hours stale that the chlorine evaporates) to 0.2 EC. Add Cal / Mag to 0.4 Ec Ph with Organic Ph - to 5.8

7/28 1220AM MONDAY.... Bubbles are looking ok, I need to trim them back to 4 colas, I am really stressing them with small space limitations, and temps at 80 to 81 7/29 Tuesday 5:45 AM💦💦 6:15 AM water with methylene blue two pills of nitric boost amino acid, five drops of Quick Sorb and I added bud factor X today and a quarter mL per liter of rhino silica💦💦💦. 1030 AM I don’t know what I’m gonna do. It turns out the bubbles plants are terrible. The stems are all very weak and they’re growing bushy at the top. I didn’t get them separated so all of the side branches grew up really long it’s completely useless. It is absolute this plant. It will be thrown in the garbage. Can you fucking believe I have to throw another entire plant in the fucking garbage😡😡😡😡. TREVOR plant cut down to 2 colas only, very top heavy not liking my grow space, had 2 more than I wanted so it's all fucked.... will remove shitty small colas and get maybe 2 good colas per plant, not expecting much. 1219pm....HOLY SHIT I FUCKED THESE.....TOOK OFF SOME COLAS.....NOT ENOUGH SPACE AND I PUSHED THEM TOO FAR.....I CAN FIX IT 7/30 1215am found a GREAT science link::: ""https://www.frontiersin.org/research-topics/8125/cannabis-genomics-breeding-and-production/magazine"". 345am PROLINE WILL BE HERE TODAY.ALL RESEARCH INDICATES PROLINE WILL PROTECT FROM HEAT STRESS!!!! 11:30 AM they are good again they are recovering from heavy defoliation half of the plant was taken off of one of them. I pushed it a little far but today they are recovering from heat and lack of air and lack of space the leaves are reaching up and they are not folding under heat stress. They look good today. Fast recovery heavy amino yesterday.👍👍👍👍🚨🚨🚨🚨 🚨🚨🚨🚨🚨🚨🚨🚨 GOT THE AMINOS AND HERE IS THE GROK RESULT OF MY MIX.......🚨🚨🚨🚨🚨🚨🚨🚨 The effects of applying essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine to cannabis plants can be significant, as these compounds act as biostimulants, influencing growth, stress resilience, and secondary metabolite production. The available research, primarily from studies on amino acids in plants, including cannabis, provides insights into their roles, though specific data on this exact combination is limited. Below, I summarize the effects of each compound on cannabis plants, drawing from relevant studies, and infer potential synergistic effects based on their known roles in plant physiology. Note that TMG (betaine) has less direct research in cannabis, so some effects are extrapolated from other plants. ### 1. Essential Amino Acids (EAAs) EAAs (e.g., leucine, lysine, methionine, etc.) are critical building blocks for proteins and play roles in plant metabolism and stress response. In cannabis: - **Growth and Development**: EAAs enhance protein synthesis, supporting shoot and root growth. Foliar or soil application of EAAs (e.g., 150–300 mg/L) increases leaf chlorophyll, shoot biomass, and root development in various plants, likely applicable to cannabis.[](https://www.nature.com/articles/s41598-022-21273-6) - **Nutrient Uptake**: EAAs like methionine and lysine improve nutrient absorption (e.g., nitrogen, iron) by acting as chelators, potentially enhancing cannabis nutrient efficiency in hydroponic or soil systems.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) - **Stress Mitigation**: EAAs, particularly methionine, support antioxidant production (e.g., glutathione), reducing oxidative stress from drought, salinity, or high light, common in cannabis cultivation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2020.00928/full)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cannabinoid/Terpene Production**: Limited evidence suggests EAAs may influence secondary metabolism. For example, methionine could enhance sulfur-containing compounds, potentially affecting terpene profiles in cannabis.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) **Application Notes**: EAAs are often applied via foliar spray (150–300 mg/L) or soil drench. Overuse may lead to nitrogen imbalance, so moderation is key.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### 2. Proline Proline is a non-essential amino acid that accumulates in plants under stress and serves multiple functions: - **Stress Tolerance**: Proline acts as an osmolyte, stabilizing cell membranes and scavenging reactive oxygen species (ROS) during drought, salinity, or temperature stress. In cannabis, exogenous proline (e.g., 50–100 mM) may enhance tolerance to environmental stressors, improving resilience in outdoor or controlled environments.[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW)[](https://pmc.ncbi.nlm.nih.gov/articles/PMC3548871/) - **Limited Growth Effect**: Proline alone is a poor nitrogen source for growth. Studies on Arabidopsis show it has a neutral or inhibitory effect on growth when used as the sole nitrogen source, suggesting it’s better for stress response than biomass accumulation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Cannabis-Specific**: Proline helps cannabis maintain water balance, potentially improving resistance to extreme temperatures, which is critical for outdoor grows.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) **Application Notes**: Foliar or root application (50–100 mg/L) is effective for stress mitigation. High doses may inhibit growth if not balanced with other nutrients.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full)[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW) ### 3. Trimethylglycine (TMG, Betaine) TMG is less studied in cannabis but known as an osmoprotectant and methyl donor in other plants: - **Stress Protection**: TMG stabilizes proteins and membranes under salinity, drought, or temperature stress, similar to proline. It may protect cannabis plants in harsh conditions, maintaining photosynthetic efficiency.[](https://www.mdpi.com/2311-7524/9/7/807) - **Methylation Support**: TMG donates methyl groups, potentially enhancing secondary metabolite synthesis (e.g., cannabinoids, terpenes), though direct evidence in cannabis is lacking. Its role in methylation could support metabolic pathways linked to EAAs and glutamine. - **Growth Effects**: Limited data suggest TMG may improve chlorophyll content and photosynthesis, indirectly supporting biomass in cannabis. **Application Notes**: TMG is typically applied at 0.5–2 g/L via foliar spray. Its effects in cannabis are inferred from other crops, so careful experimentation is needed. ### 4. Glutamine Glutamine is a conditionally essential amino acid central to nitrogen metabolism: - **Growth Promotion**: Glutamine (10–1000 mg/L) significantly enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and hibiscus, likely benefiting cannabis vegetative growth. In cannabis, it may support lush foliage and robust rooting in hydroponics.[](https://www.scielo.br/j/oh/a/GZxMgqXb9wm7bsCTv4WwPdc/?lang=en)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html?lang=en) - **Nitrogen Assimilation**: As a primary nitrogen acceptor, glutamine improves nitrogen use efficiency via the glutamine synthetase/glutamate synthase cycle, enhancing nutrient uptake in cannabis.[](https://academic.oup.com/jxb/article/58/9/2339/544408)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Stress Response**: Glutamine supports proline synthesis, boosting stress tolerance. It also contributes to chlorophyll and protein synthesis, improving photosynthesis and yield potential in cannabis.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Secondary Metabolism**: Glutamine is a precursor to glutamate, which influences GABA, arginine, and proline synthesis, potentially affecting cannabinoid or terpene production indirectly.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) **Application Notes**: Foliar sprays (250–1000 mg/L) or root application twice weekly are effective. Glutamine at 250 mg/L combined with other amino acids (e.g., glycine) shows synergistic growth effects.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) ### Synergistic Effects on Cannabis - **Growth and Yield**: The combination of EAAs, glutamine, and proline may enhance vegetative growth and biomass. Glutamine and EAAs drive protein synthesis and nitrogen uptake, while proline supports tissue integrity under stress. TMG likely amplifies these effects by improving cellular hydration and methylation, potentially increasing leaf area and bud quality.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.nature.com/articles/s41598-022-21273-6) - **Stress Resilience**: Proline and TMG act as osmoprotectants, while EAAs and glutamine bolster antioxidant systems, collectively improving cannabis resilience to drought, salinity, or temperature extremes common in cultivation.[](https://www.mdpi.com/2311-7524/9/7/807) - **Secondary Metabolites**: Glutamine and TMG may enhance cannabinoid and terpene production by supporting metabolic pathways (e.g., methylation, nitrogen metabolism). EAAs like methionine could influence sulfur-based terpenes. However, direct evidence for cannabinoid enhancement is sparse.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Nutrient Efficiency**: Glutamine and EAAs improve nitrogen and micronutrient uptake, while TMG may enhance nutrient transport, optimizing cannabis growth in nutrient-limited systems like hydroponics.[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) ### Potential Risks and Considerations - **Inhibition at High Doses**: High concentrations of proline or certain EAAs (e.g., lysine, valine) can inhibit growth if used as sole nitrogen sources. Balanced mixtures mitigate this, as glutamine can antagonize inhibitory effects of proline or other amino acids.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Nitrogen Overload**: Excessive EAAs or glutamine may lead to nitrogen toxicity, causing leaf burn or stunted growth in cannabis. Monitor plant response and maintain balanced nutrient solutions.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **TMG Metabolism**: TMG may increase trimethylamine production in the soil microbiome, potentially affecting cannabis root health if not managed. Healthy soil microflora can mitigate this.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **Application Timing**: Foliar sprays (150–500 mg/L for EAAs, glutamine, proline; 0.5–2 g/L for TMG) during vegetative growth or early flowering maximize benefits. Avoid overuse during late flowering to prevent nutrient imbalances affecting bud quality. ### Cannabis-Specific Insights - **Hydroponics and Soil**: In hydroponic systems, EAAs and glutamine ensure efficient nitrogen delivery, while proline and TMG support stress resistance in fluctuating pH or nutrient conditions. In soil, maintaining microflora is crucial, as it naturally supplies amino acids; supplementation should be moderate.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cultivar Variability**: Cannabis cultivars differ in nutrient demands and stress responses. Sativa-dominant strains may benefit more from proline and TMG for stress tolerance, while indica strains may respond better to EAAs and glutamine for dense bud growth. Testing is needed. - **Secondary Metabolites**: While amino acids influence secondary metabolism, direct effects on THC or CBD levels are unconfirmed. Glutamine and TMG may indirectly enhance terpene diversity via improved photosynthesis and methylation.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) ### Practical Recommendations "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨 - **Dosing**: Apply EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) via foliar spray or root drench twice weekly during vegetative growth and early flowering. - **Combination**: Use a balanced mix (e.g., 250 mg/L EAAs + 250 mg/L glutamine + 50 mg/L proline + 500 mg/L TMG) to avoid inhibition. Test on a small batch first.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Monitoring**: Check for signs of nitrogen toxicity (dark green leaves, clawing) or stress relief (improved vigor, greener leaves). Adjust doses based on plant response. "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to enhance uptake.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient lockout. In soil, ensure healthy microbial activity to complement amino acid supplementation.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### Evidence Gaps - **Specific Combination**: No studies directly address EAAs, proline, TMG, and glutamine together in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid Impact**: Limited data on how these compounds affect THC/CBD levels. More research is needed on secondary metabolite pathways.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **TMG in Cannabis**: TMG’s effects are extrapolated from other crops. Cannabis-specific trials are needed to confirm benefits. ### Conclusion Applying EAAs, proline, TMG, and glutamine to cannabis plants can enhance growth, stress tolerance, and nutrient uptake, with potential benefits for yield and secondary metabolite production. EAAs and glutamine drive biomass and nitrogen efficiency, proline and TMG bolster stress resilience, and their combination may optimize cannabis health in challenging environments. However, careful dosing and monitoring are crucial to avoid inhibition or toxicity. For best results, test small-scale applications and consult with a horticulturist familiar with cannabis cultivation. If you want more details on application methods, specific cultivars, or further searches for cannabis-related studies, let me know!""🚨🚨🚨🚨 🚨🚨 7/31 125am!!!!! Adding L-glycine to the combination of essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine can further influence the effects on cannabis plants, as glycine is a non-essential amino acid with unique roles in plant physiology. Below, I outline the potential effects of L-glycine alone and in combination with the previously mentioned compounds, focusing on their impact on cannabis growth, stress response, and secondary metabolite production. Since specific studies on this exact combination in cannabis are lacking, I’ll integrate findings from plant science and infer synergistic effects based on known mechanisms. I’ll keep it concise and tailored to cannabis cultivation. ### Effects of L-Glycine on Cannabis Plants L-Glycine is a simple amino acid involved in protein synthesis, stress response, and metabolic pathways: - **Growth Promotion**: Glycine (50–500 mg/L) enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and rice, likely benefiting cannabis vegetative growth. It serves as a nitrogen source and supports protein synthesis, complementing EAAs and glutamine. - **Stress Tolerance**: Glycine acts as a precursor to glutathione, a key antioxidant, reducing oxidative stress from drought, salinity, or high light. In cannabis, it may improve resilience in challenging environments (e.g., outdoor grows or high-intensity indoor setups). - **Secondary Metabolism**: Glycine is a precursor to purines and chlorophyll, potentially enhancing photosynthesis and energy metabolism. It may indirectly influence cannabinoid and terpene production by supporting metabolic pathways, though direct evidence in cannabis is limited. - **Nutrient Uptake**: Glycine chelates micronutrients (e.g., iron, zinc), improving their availability, which is critical for cannabis in hydroponic or nutrient-poor soil systems. **Application Notes**: Foliar sprays or root drenches (100–500 mg/L) are effective, often applied twice weekly during vegetative growth or early flowering. ### Synergistic Effects with EAAs, Proline, TMG, and Glutamine Adding L-glycine to the mix may enhance the effects on cannabis plants in the following ways: 1. **Enhanced Growth and Biomass**: - **Glycine + EAAs/Glutamine**: Glycine complements EAAs and glutamine in protein synthesis and nitrogen metabolism. Studies show glycine (250 mg/L) combined with glutamine enhances shoot and root growth in plants, likely amplifying cannabis vegetative vigor and bud development. - **Synergy**: Glycine’s role in chlorophyll synthesis boosts photosynthesis, supporting the energy demands of EAA-driven protein synthesis and glutamine’s nitrogen assimilation, potentially increasing leaf area and yield. 2. **Improved Stress Resilience**: - **Glycine + Proline/TMG**: Glycine and proline both contribute to glutathione production, enhancing antioxidant defenses. TMG’s osmoprotectant properties further stabilize cells under stress. Together, they may improve cannabis tolerance to drought, heat, or salinity, maintaining growth in adverse conditions. - **Cannabis-Specific**: This combination could be particularly beneficial for outdoor cannabis or indoor setups with inconsistent temperature/humidity, reducing leaf wilting or nutrient stress. 3. **Secondary Metabolite Production**: - **Glycine + Glutamine/TMG**: Glycine and glutamine are precursors to metabolic pathways (e.g., GABA, purines) that may influence cannabinoid and terpene synthesis. TMG’s methyl donation could enhance these pathways by supporting enzyme activity. While direct evidence is sparse, this stack may increase terpene diversity or cannabinoid yield in cannabis buds. - **EAAs Contribution**: Methionine (an EAA) and glycine may support sulfur-containing compounds, potentially enriching terpene profiles. 4. **Nutrient Efficiency**: - **Glycine + EAAs/Glutamine**: Glycine’s chelating properties enhance micronutrient uptake, complementing EAAs and glutamine’s role in nitrogen assimilation. This could optimize nutrient use in hydroponic cannabis systems, reducing nutrient lockout. - **TMG Synergy**: TMG’s role in cellular hydration may improve nutrient transport, amplifying glycine’s effects on micronutrient availability. ### Potential Risks and Considerations - **Inhibition at High Doses**: High glycine doses (1000 mg/L) may inhibit growth by disrupting nitrogen balance, especially if combined with EAAs or glutamine. Studies on Arabidopsis show glycine can be toxic as a sole nitrogen source, so balanced mixtures are crucial. - **Nitrogen Overload**: Adding glycine to EAAs and glutamine increases nitrogen input, risking toxicity (e.g., leaf burn, stunted growth) in cannabis. Monitor for dark green leaves or clawing and adjust doses. - **Competition for Absorption**: Glycine, EAAs, and glutamine may compete for root or foliar uptake, reducing efficacy. Split applications (e.g., glycine in the morning, EAAs/glutamine post-light cycle) can help. - **TMG Interaction**: Glycine and TMG both influence methylation pathways, but excessive methyl donors could disrupt metabolic balance in sensitive plants. Stick to moderate doses (e.g., 100–500 mg/L glycine, 0.5–1 g/L TMG). - **Cultivar Variability**: Cannabis strains (e.g., sativa vs. indica) differ in nutrient and stress responses. Glycine’s benefits may be more pronounced in stress-prone sativas or nutrient-sensitive hybrids. ### Practical Recommendations - **Dosing**: Combine glycine (100–500 mg/L), EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) in a balanced foliar spray or root drench. Apply twice weekly during vegetative growth and early flowering. - **Application**: Use foliar sprays for rapid uptake during vegetative stages or stress events. Root drenches suit hydroponic systems. Test on a small batch to assess plant response. - **Monitoring**: Watch for nitrogen toxicity (dark, curled leaves) or stress relief (greener, more vigorous plants). Adjust concentrations based on cultivar and growth stage. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to maximize glycine’s chelating benefits. Ensure adequate magnesium for chlorophyll synthesis. - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient imbalances. In soil, maintain healthy microbial activity, as microbes naturally produce glycine and other amino acids. ### Evidence Gaps - **Specific Combination**: No studies address glycine combined with EAAs, proline, TMG, and glutamine in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid/Terpene Impact**: While glycine may support secondary metabolism, direct effects on THC/CBD or terpene levels are unconfirmed. Cannabis-specific trials are needed. - **TMG and Glycine**: Their combined effects on methylation in cannabis are speculative, as most TMG research focuses on other crops. ### Conclusion Adding L-glycine to EAAs, proline, TMG, and glutamine likely enhances cannabis growth, stress tolerance, and nutrient efficiency by supporting protein synthesis, antioxidant production, and micronutrient uptake. The combination may improve vegetative vigor, bud quality, and resilience to environmental stressors, with potential (but unconfirmed) benefits for cannabinoid and terpene production. Careful dosing and monitoring are essential to avoid nitrogen toxicity or absorption competition. Start with low doses, test on a small scale, and consult a cannabis cultivation expert for tailored advice. If you want me to search for more studies, focus on a specific aspect (e.g., glycine’s role in terpene synthesis), or provide application protocols for a particular cannabis strain, let me know!"""

Almost Chop Day for wa girl MS PURPLE! ✂️ The last few days were all about that flush life runoff nice and low, just how we like it ✅ She’s been an absolute tank from day one, taking almost everything thrown her way like a champ 💪🏾🔥 By the end of the week, most of the buds turned purple, most of her fan leaves faded to yellow, and after a solid trichome check..mostly milky with just the right touch of amber. Nailed it 🎯 And the smell? Straight adorable 🤗😍 I’ve got other strains on their own journeys, posted weekly in my diary feel free to take a little tour Thanks for riding along with me on this one 🙏🏾 Right after this photo session she’s off the a better world Happy growing 💚

It's day 54 for the Tropical Ice Cream One🍦 and day 11 since switching to 12/12. No signs of flowering yet so I'll still count this week as vegetative growth. She grew quite a bit so flowering should start any day now. Overall she seems quite happy despite the higher temperatures (~33°C peak) but she did show some taco leaves so I've turned the exhaust higher. But I'm not too worried. Looking forward to week ~4-5 when she should start to smell :) Thanks for checking in and see you next week. Happy growing 🌱

Pretty cool....i have two males of each cultivar to choose from as well as a female from each. The 3 females will get there own diaries. Males- -Koffee F8 #2 is just dumping pollen like crazy. Way more then #3 and has a fatter "Flower" structure #3 has thinner "Flower" structure but is showing more purple then the #2. I have plates colleting the pollen the best i can. Ive never grown a male plant past 3 weeks. This is exciting. -Clover Shake #1 male is very thin #3 male is now showing sex and will go into the male tent -Candy Master Star Dawg #2 male #3 male Both going into the male tent

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