9/15 12AM MONDAY!!! THEY ARE ALL DOING GREAT!!! VERY HEAVY AMINOS YESTERDAY, NO DAMAGE. 9/15 11:24PM. In plant cells, glutathione S-transferases (GSTs) act as transporters, facilitating the movement of anthocyanins from the endoplasmic reticulum (ER) to the vacuole for storage, though their exact mechanism is still being studied. The synthesis of glutathione (GSH) itself occurs in the cytosol of mammalian cells through two ATP-requiring enzymatic steps, while anthocyanins are synthesized via a separate phenylpropanoid pathway 9/15 11:42PM glutathione metabolism glutathione S-transferases plantsThinkingKicking off 5 searchesLooking at 71 sitesPutting it all together In plants, glutathione S-transferases (GSTs) are a diverse family of enzymes integral to glutathione (GSH) metabolism, primarily functioning in detoxification and stress response. The core of this process involves GSTs catalyzing the conjugation of the tripeptide GSH with various toxic compounds, or xenobiotics, making them less harmful and easier to sequester or transport. Glutathione (GSH) metabolism in plants GSH metabolism is a tightly regulated process that maintains cellular redox balance and protects against stress. Biosynthesis: GSH is synthesized in two adenosine triphosphate (ATP)-dependent steps:(gamma )-Glutamylcysteine synthetase (GSH1) joins L-glutamate and L-cysteine to form (gamma )-glutamylcysteine. In Arabidopsis, this enzyme is located exclusively in the chloroplasts.Glutathione synthetase (GSH2) adds glycine to (gamma )-glutamylcysteine to produce GSH. This enzyme is found in both the cytosol and the chloroplasts.Oxidation and reduction: GSH exists in two forms: a reduced monomer (GSH) and an oxidized disulfide dimer (GSSG).Oxidation: In the process of scavenging reactive oxygen species (ROS), GSH is oxidized to GSSG.Reduction: Glutathione reductase (GR) uses NADPH to reduce GSSG back into active GSH, which is essential for maintaining a high GSH-to-GSSG ratio inside the cell.Degradation: Glutathione can be degraded in the cytosol, apoplast, and vacuoles by (gamma )-glutamyltransferases (GGTs) and other peptidases, allowing for recycling of its amino acid components.Transport and compartmentalization: While synthesis occurs in the chloroplasts and cytosol, transporters move GSH and its conjugates throughout the cell, including to the mitochondria, peroxisomes, and vacuoles. Functions of glutathione S-transferases (GSTs) GSTs are a large, ancient gene family with diverse roles that extend beyond detoxification. The primary functions involving GSH are: Detoxification of xenobiotics and heavy metals: GSTs catalyze the conjugation of the thiol group of GSH to a wide range of electrophilic and hydrophobic substrates, including herbicides, pesticides, and heavy metals. The resulting GSH-conjugates are then recognized by ATP-binding cassette (ABC) transporters and sequestered in the vacuole, reducing their cellular toxicity.Protection from oxidative stress: Stress conditions like drought, salinity, and high light increase levels of ROS, which cause lipid peroxidation. 🚨Some GST isoforms have glutathione peroxidase (GPOX) activity, using GSH to reduce toxic lipid hydroperoxides to less damaging alcohols, thereby protecting cell membranes.Defense against pathogens: During pathogen attacks, GST activity increases to detoxify microbial toxins and secondary defense-related compounds. This helps to bolster the plant's resistance response.Metabolism of endogenous compounds: GSTs play roles beyond immediate stress response by participating in the normal metabolic processes of plants. Examples include:Hormone transport: Some GSTs act as binding proteins (ligandins) that transport plant hormones like auxins within the cell.Metabolite transport: Specific GSTs facilitate the transport of important compounds like flavonoids and anthocyanin pigments into the vacuole.Tyrosine degradation: The zeta GST class (GSTZ) catalyzes a critical step in the breakdown of the amino acid tyrosine.Redox signaling: By influencing the GSH/GSSG ratio and interacting with plant hormones, GSTs can regulate cellular signaling pathways that control growth and stress response. 🚨GSTs and plant stress response The expression of many GST genes is highly inducible by various environmental and endogenous cues, making them key players in a plant's ability to adapt to adverse conditions. Abiotic stress: Drought, salinity, heavy metals, and extreme temperatures all trigger increased GST expression, which helps plants manage resulting oxidative damage. Overexpression of specific GST genes has been shown to enhance stress tolerance in transgenic plants.Herbicide resistance: The ability of crops and weeds to detoxify herbicides through GST-catalyzed conjugation is a well-studied example of GSTs in stress adaptation. Herbicide safeners, chemical agents that protect crops from herbicide injury, work by boosting the activity of these GST enzymes.Developmental processes: Studies have linked the expression of certain GST genes to specific stages of plant development, such as fruit ripening, root and stem development, and flower formation. "" 9/16 12:32am AI Overview In plants, phenylalanine is the precursor for anthocyanin synthesis, a process that involves a multistep pathway in which glutathione (GSH) plays a critical role in the transport of the final pigment to the cell vacuole. Phenylalanine initiates anthocyanin synthesis The biosynthesis of anthocyanins begins with the amino acid phenylalanine, which is produced via the shikimate pathway. The conversion process, known as the phenylpropanoid pathway, involves a series of enzymatic reactions: 1. Phenylalanine ammonia-lyase (PAL): This enzyme catalyzes the first committed step of the pathway, converting L-phenylalanine into trans-cinnamic acid. 2. Downstream enzymatic reactions: A series of additional enzymes, such as chalcone synthase and chalcone isomerase, convert the intermediate compounds into unstable anthocyanidins. 3. Glycosylation: Glycosyltransferase enzymes stabilize the anthocyanidins by adding sugar molecules, creating the final, stable anthocyanin pigments. 4. Glutathione is crucial for anthocyanin transport After synthesis, glutathione is essential for transporting anthocyanins for storage. * Glutathione S-transferases (GSTs): These enzymes bind to the newly formed anthocyanins in the cytoplasm. This binding is a key step that facilitates the transport of the anthocyanin-GST complex across the tonoplast (the vacuolar membrane) and into the vacuole. * Vacuolar storage: Once inside the vacuole, the anthocyanins accumulate and provide color to plant parts, such as flowers, fruits, and leaves. * Catalytic role: Recent research has also found that certain GSTs have a catalytic role in anthocyanin synthesis. They can catalyze a dehydration step to form specific anthocyanidins, significantly boosting production in engineered systems. * The process in summary The synthesis and accumulation of anthocyanins involve a coordinated process of multiple steps: 1. Production from phenylalanine: The amino acid phenylalanine enters the phenylpropanoid pathway, where enzymes convert it into the basic flavonoid structures. 2. Formation of anthocyanidins: Further enzymatic modifications produce the colored but unstable anthocyanidins. 3. Stabilization and transport with glutathione: These anthocyanidins are stabilized by glycosylation and then bound by GSTs (which use glutathione). This binding enables their transport into the vacuole for storage and visible pigmentation. 9/15 12:46AM https://pmc.ncbi.nlm.nih.gov/articles/PMC7238016/ 9/16 1:30AM BKO is looking great!!! Conclusion The “butter frosting” resin on Cookie Apple F1, healthy yellow-green fusiform, and Blueberry KO’s pigmented cotyledons show your anthocyanin-glutathione-phenylalanine strategy is working—phenylalanine drives synthesis, glutathione ensures transport. Tweak amino acids to 100–150 mg/L to reduce tip burn. 9/16 3:34am 9/16 4:31AM Anthocyanin glutathione synthesis phenylalanine proline tmg powder relating current project: * Phenylalanine is a precursor: Phenylalanine is an amino acid and the starting point for the phenylpropanoid pathway in plants. * Anthocyanin synthesis: This pathway creates various secondary metabolites, including the flavonoid pigments known as anthocyanins, which give plants their red, purple, and blue colors. * Pathway activation: Multiple enzymes, such as phenylalanine ammonia-lyase (PAL), catalyze the conversion of phenylalanine into the building blocks for anthocyanin. * Anthocyanin and glutathione synthesis * Glutathione S-transferase (GST): This enzyme is crucial for synthesizing anthocyanins in plants. It transports anthocyanins into the cell's vacuole for storage. * Glutathione (GSH) production: Anthocyanins can promote glutathione synthesis in certain cells. For instance, the anthocyanin cyanidin-3-O-β-glucoside (C3G) has been shown to increase the expression of glutamate-cysteine ligase (Gclc), a key enzyme in GSH synthesis. * Antioxidant effect: By upregulating GSH production, anthocyanins contribute to the antioxidant defense system, protecting cells from oxidative stress. * Proline and glutathione synthesis * Shared precursor: In some organisms, the synthesis pathways for proline and glutathione share a precursor molecule called γ-glutamyl phosphate. * Alternative pathway: Research on bacteria and yeast has revealed an alternative, trace pathway where γ-glutamyl phosphate from the proline synthesis pathway can be diverted to produce the glutathione precursor γ-glutamylcysteine. * Oxidative stress response: Studies in mouse oocytes show that supplementing with proline upregulates genes related to glutathione synthesis (Gclc and Gclm), increases glutathione levels, and reduces oxidative stress. * TMG powder, methylation, and homocysteine * Methyl donor: Trimethylglycine (TMG), or betaine, is a potent methyl donor, meaning it provides methyl groups needed for various biochemical processes in the body, including the methylation cycle. * Homocysteine regulation: One of TMG's primary functions is to convert the amino acid homocysteine into methionine. This helps regulate homocysteine levels, which is important for cardiovascular health. * TMG and proline interaction: In plants and some organisms, TMG and proline act as compatible solutes or osmoprotectants, helping cells stabilize against osmotic stress like drought or salinity. However, in human biology, TMG mainly functions through methylation, while proline is involved in different metabolic and antioxidant roles. * How they all relate The listed components are connected through several overlapping metabolic and regulatory pathways: * Anthocyanin synthesis starts with phenylalanine. * Anthocyanins can promote glutathione synthesis via upregulation of key enzymes like Gclc. * Glutathione synthesis can be influenced by the proline synthesis pathway, as they share an intermediate in some contexts. * TMG powder supports the methylation cycle, which helps regulate homocysteine levels. While TMG and proline serve similar protective roles in some organisms, their primary human metabolic functions differ, with TMG focusing on methylation and proline having distinct roles in antioxidant response and metabolism 9/17 217am Die Hard Christmas Grow 9/18, 11:34 AM. I ordered some square saucers that were cartoonishly too small but they fit inside the AC infinity germination kit and they fit with the Bud Cups perfectly really nice so it’s not a total loss. 9/18 11:45AM mix. Foliar Spray, the rest of the mix ec 0.46 Mixed up Aminos first and separate and use 16 oz for foliar spray. Then mixed up: Root: 1 mL/L Connoisseur A & B GROW, .2 mL/L CaliMagic, .2 mL/L Purpinator. Setria Glutathione: 150 mg/L(Brand: Emerald 250mg capsule.) TMG: 150 mg/L = (Brand Nutricost) Phenylalanine: 150 mg/L (Brand Nutricost) Proline: 150 mg/L (Brand Nutricost). 9/18 228PM AI Overview Glutathione influences plant colors by regulating the accumulation of pigmented compounds, primarily anthocyanins. The tripeptide accomplishes this through its role in transporting pigments within plant cells and in protecting against environmental stresses like UV radiation that can cause oxidative damage. Transporting pigments into plant cell vacuoles Glutathione works with a class of enzymes called Glutathione S-transferases (GSTs) to transport pigments like anthocyanins into the vacuole for storage. Anthocyanin transport: In plants with pigmented tissues, such as purple grapes or red flowers, glutathione-conjugated pigments are transported by GSTs across the tonoplast membrane into the vacuole. This process is crucial for the stable accumulation of pigments. Genetic manipulation: Research shows that manipulating specific GST genes can alter a plant's pigmentation. For instance, silencing a particular GST gene in purple grape hyacinths caused their petal color to shift to a lighter shade of purple. Similarly, defective GST genes in carnations resulted in pale-colored flowers. Protecting against UV light and stress Glutathione helps regulate plant pigmentation in response to environmental factors, especially UV-B radiation. Activating flavonoid production: When plants are exposed to UV light, a surge in glutathione triggers the expression of genes involved in producing flavonoids. Flavonoids, including anthocyanins, can act as protective sunscreens for the plant, and their increased synthesis and accumulation can alter visible coloration. Balancing oxidative stress: Intense UV-B radiation increases reactive oxygen species (ROS) in plants, which can cause oxidative damage. Glutathione is a master antioxidant that helps detoxify these ROS, preventing cellular damage that can affect a plant's pigment-producing mechanisms. Indirectly influencing plant colors By regulating cellular redox status and interacting with other molecules, glutathione also affects pigment expression in more indirect ways. The xanthophyll cycle: As part of a plant's antioxidant system, glutathione helps maintain the reduced state of other protective antioxidants like tocopherol and zeaxanthin. Zeaxanthin is a carotenoid pigment involved in the xanthophyll cycle, which helps dissipate excess light energy. Redox signaling: The balance between reduced glutathione (GSH) and oxidized glutathione (GSSG) is a key cellular signal for stress response. A shift in this ratio during environmental stress can influence the production of secondary metabolites like pigments, allowing the plant to adapt. " 9/19 1:41AM AI Overview The key difference is that anthocyanins are the sugar-containing form (glycosides) of pigments, while anthocyanidins are the sugar-free form (aglycones). Anthocyanidins are the foundational molecules, and when a sugar molecule attaches to them, they become anthocyanins, which are more stable and water-soluble, making them the forms found naturally in plants, such as berries and purple vegetables. Anthocyanidin (Aglycone) Structure: The basic, sugar-free molecule of the anthocyanin structure. Location: Not found freely in nature but is the core component that is then glycosylated. Properties: Color changes with pH, being visible in acidic conditions but colorless in basic conditions. Examples: Cyanidin, delphinidin, pelargonidin, peonidin, petunidin, and malvidin. Anthocyanin (Glycoside) Structure: Consists of an anthocyanidin linked to one or more sugar molecules. Location: Found in the vacuoles of plant cells. Properties: Water-soluble and are the pigments responsible for the red, purple, and blue colors in plants. Function: The sugar attached provides stability, allowing for accumulation in plants and providing antioxidant properties. Examples: Cyanidin-3-glucoside and other derivatives like acylated anthocyanins. " 9/19 2:43AM I also saw a good mans immediate accent into Heaven, that perspective matters too. no less angry about it though. 9/19 10AM Heavy Amino spray 250mg each in 1L of Setria Glutathione and Phenylalanine 9/19 10:10PM The sons and daughters of americas real terror organization carried out the last horror show, and the one before that and before that. It's not a foreign country, it's always the anti white anti human black sheets and badges that did this. 9/19 10:43PM AI Overview Phenylalanine and glutathione contribute to plant colors through different biochemical pathways . Phenylalanine is a precursor for the pigments themselves, primarily anthocyanins, while glutathione is involved in the transport and stabilization of these pigments within the plant cell. Phenylalanine: The pigment precursor The source of aromatic compounds: Phenylalanine is an aromatic amino acid and the starting compound for the phenylpropanoid pathway in plants. This pathway produces a vast number of secondary metabolites, including anthocyanins, which give many plants their red, purple, and blue colors. Color enhancement: Research has shown that increasing the amount of available phenylalanine can lead to more intense red coloration in some fruits, such as mangoes and apples. Pathway stimulation: Phenylalanine ammonia-lyase (PAL) is a key enzyme in this pathway that converts phenylalanine into precursors for anthocyanin biosynthesis. An increase in phenylalanine levels stimulates this entire process. Glutathione: The pigment transporter Anthocyanin transport: After anthocyanin pigments are synthesized in the cell's cytoplasm, they must be transported into the central vacuole for storage. Glutathione S-transferases (GSTs) are a family of enzymes that facilitate this process. Color intensity: A functional GST is essential for proper anthocyanin accumulation. Mutations in GST genes can result in a significant decrease in color intensity, as seen in the pale or white flowers of certain gentian mutants. Mechanism of action: The GST binds to the anthocyanin pigment and moves it from the cytoplasm into the vacuole. If the GST is defective, the pigment cannot be transported correctly, leading to a loss of coloration. Summary of interactions The roles of phenylalanine and glutathione are distinct but cooperative in producing plant colors: Phenylalanine provides the essential building blocks for creating the colored compounds (anthocyanins). Glutathione, with the help of GSTs, ensures these colored compounds are correctly moved to their storage location (the vacuole). This cooperative system explains why mutations or deficiencies in either process can lead to reduced or altered coloration in plants." 9/19 10:51PM AI Overview. Trimethylglycine (TMG) supports the production of glutathione, a vital antioxidant . However, there is no evidence to suggest a direct relationship between these supplements, the amino acid phenylalanine, and the colors of plants. The concepts are linked indirectly via complex biological processes. Trimethylglycine (TMG) and glutathione TMG and glutathione are connected through the body's methylation cycle. TMG as a methyl donor: TMG plays a critical role in the methylation process by donating a methyl group, which helps convert the harmful amino acid homocysteine back into methionine. Support for glutathione production: This methylation cycle, which is supported by TMG, is essential for producing glutathione. Glutathione is a powerful antioxidant that protects against cellular damage and is crucial for detoxification. Supplementation considerations: TMG supplements are sometimes taken alongside other supplements to support health, though consultation with a healthcare provider is recommended. Phenylalanine and the methylation cycle Phenylalanine is an essential amino acid, but its role is distinct from the TMG-glutathione process. Essential nutrient: Phenylalanine is a key nutrient for cellular metabolism. Potential interactions: A separate medical study on experimental hyperphenylalaninemia (abnormally high phenylalanine levels) in chicks observed a decrease in other amino acids in the brain, including those involved in the glutathione pathway. This demonstrates how excessive levels of one amino acid can potentially influence others, though this does not represent a typical interaction. Plant colors The connection between the supplements and plant colors is purely conceptual, as the colors are determined by completely different biological processes. Anthocyanins: The colors of many plants, including red, purple, and blue flowers, come from pigments called anthocyanins. Glutathione in plants: While plants contain glutathione as an antioxidant to combat stress, it influences color by regulating the transport and accumulation of anthocyanin pigments, not by being a pigment itself. TMG and phenylalanine in plants: Plants contain TMG, which functions as an osmoprotectant (protecting against osmotic stress). They also contain phenylalanine, but these substances do not directly determine the plant's visible color." 9/19 11:21PM. !!!!!! this was pointless and im dumber for having read it. !!! Light intensity and spectrum affect metabolism of glutathione and amino acids at transcriptional level: https://pmc.ncbi.nlm.nih.gov/articles/PMC6938384/ 9/20 11:08 AM the seedlings and the four autos are doing just great. The amino spray with phenylene and glutathione really had nice effects no burning nice solid growth even seedlings from basil lavender various lettuce all are perfect.🚨🚨🚨👍👍👍👍👍 9/21 2AM I AM BECOME ANTHOCYANID!!! ITS WORKING AND ON A SEEDLING I SEE THE GELATO COLLORS IN BLUEBERRY KO AND THE LEAF SHAPE OF BUBBLES BLUEBERRY,!!!

White Widow is officially in flower now!! Nice little bud sites developing. Also started using Mammoth P this week. I'm excited to see what Mammoth P does with my girls, the reviews have been pretty outstanding!

What's in the soil? What's not in the soil would be an easier question to answer. 16-18 DLI @ the minute. +++ as she grows. Probably not recommended, but to get to where it needs to be, I need to start now. Vegetative @1400ppm 0.8–1.2 kPa 80–86°F (26.7–30°C) 65–75%, LST Day 10, Fim'd Day 11 CEC (Cation Exchange Capacity): This is a measure of a soil's ability to hold and exchange positively charged nutrients, like calcium, magnesium, and potassium. Soils with high CEC (more clay and organic matter) have more negative charges that attract and hold these essential nutrients, preventing them from leaching away. Biochar is highly efficient at increasing cation exchange capacity (CEC) compared to many other amendments. Biochar's high CEC potential stems from its negatively charged functional groups, and studies show it can increase CEC by over 90%. Amendments like compost also increase CEC but are often more prone to rapid biodegradation, which can make biochar's effect more long-lasting. biochar acts as a long-lasting Cation Exchange Capacity (CEC) enhancer because its porous, carbon-rich structure provides sites for nutrients to bind to, effectively improving nutrient retention in soil without relying on the short-term benefits of fresh organic matter like compost or manure. Biochar's stability means these benefits last much longer than those from traditional organic amendments, making it a sustainable way to improve soil fertility, water retention, and structure over time. Needs to be charged first, similar to Coco, or it will immobilize cations, but at a much higher ratio. a high cation exchange capacity (CEC) results in a high buffer protection, meaning the soil can better resist changes in pH and nutrient availability. This is because a high CEC soil has more negatively charged sites to hold onto essential positively charged nutrients, like calcium and magnesium, and to buffer against acid ions, such as hydrogen. EC (Electrical Conductivity): This measures the amount of soluble salts in the soil. High EC levels indicate a high concentration of dissolved salts and can be a sign of potential salinity issues that can harm plants. The stored cations associated with a medium's cation exchange capacity (CEC) do not directly contribute to a real-time electrical conductivity (EC) reading. A real-time EC measurement reflects only the concentration of free, dissolved salt ions in the water solution within the medium. 98% of a plants nutrients comes directly from the water solution. 2% come directly from soil particles. CEC is a mediums storage capacity for cations. These stored cations do not contribute to a mediums EC directly. Electrical Conductivity (EC) does not measure salt ions adsorbed (stored) onto a Cation Exchange Capacity (CEC) site, as EC measures the conductivity of ions in solution within a soil or water sample, not those held on soil particles. A medium releases stored cations to water by ion exchange, where a new, more desirable ion from the water solution temporarily displaces the stored cation from the medium's surface, a process also seen in plants absorbing nutrients via mass flow. For example, in water softeners, sodium ions are released from resin beads to bond with the medium's surface, displacing calcium and magnesium ions which then enter the water. This same principle applies when plants take up nutrients from the soil solution: the cations are released from the soil particles into the water in response to a concentration equilibrium, and then moved to the root surface via mass flow. An example of ion exchange within the context of Cation Exchange Capacity (CEC) is a soil particle with a negative charge attracting and holding positively charged nutrient ions, like potassium (K+) or calcium (Ca2+), and then exchanging them for other positive ions present in the soil solution. For instance, a negatively charged clay particle in soil can hold a K+ ion and later release it to a plant's roots when a different cation, such as calcium (Ca2+), is abundant and replaces the potassium. This process of holding and swapping positively charged ions is fundamental to soil fertility, as it provides plants with essential nutrients. Negative charges on soil particles: Soil particles, particularly clay and organic matter, have negatively charged surfaces due to their chemical structure. Attraction of cations: These negative charges attract and hold positively charged ions, or cations, such as: Potassium (K+) Calcium (Ca2+) Magnesium (Mg2+) Sodium (Na+) Ammonium (NH4+) Plant roots excrete hydrogen ions (H+) through the action of proton pumps embedded in the root cell membranes, which use ATP (energy) to actively transport H+ ions from inside the root cell into the surrounding soil. This process lowers the pH of the soil, which helps to make certain mineral nutrients, such as iron, more available for uptake by the plant. Mechanism of H+ Excretion Proton Pumps: Root cells contain specialized proteins called proton pumps (H+-ATPases) in their cell membranes. Active Transport: These proton pumps use energy from ATP to actively move H+ ions from the cytoplasm of the root cell into the soil, against their concentration gradient. Role in pH Regulation: This active excretion of H+ is a major way plants regulate their internal cytoplasmic pH. Nutrient Availability: The resulting decrease in soil pH makes certain essential mineral nutrients, like iron, more soluble and available for the root cells to absorb. Ion Exchange: The H+ ions also displace positively charged mineral cations from the soil particles, making them available for uptake. Iron Uptake: In response to iron deficiency stress, plants enhance H+ excretion and reductant release to lower the pH and convert Fe3+ to the more available form Fe2+. The altered pH can influence the activity and composition of beneficial microbes in the soil. The H+ gradient created by the proton pumps can also be used for other vital cell functions, such as ATP synthesis and the transport of other solutes. The hydrogen ions (H+) excreted during photosynthesis come from the splitting of water molecules. This splitting, called photolysis, occurs in Photosystem II to replace the electrons used in the light-dependent reactions. The released hydrogen ions are then pumped into the thylakoid lumen, creating a proton gradient that drives ATP synthesis. Plants release hydrogen ions (H+) from their roots into the soil, a process that occurs in conjunction with nutrient uptake and photosynthesis. These H+ ions compete with mineral cations for the negatively charged sites on soil particles, a phenomenon known as cation exchange. By displacing beneficial mineral cations, the excreted H+ ions make these nutrients available for the plant to absorb, which can also lower the soil pH and indirectly affect its Cation Exchange Capacity (CEC) by altering the pool of exchangeable cations in the soil solution. Plants use proton (H+) exudation, driven by the H+-ATPase enzyme, to release H+ ions into the soil, creating a more acidic rhizosphere, which enhances nutrient availability and influences nutrient cycling processes. This acidification mobilizes insoluble nutrients like iron (Fe) by breaking them down, while also facilitating the activity of beneficial microbes involved in the nutrient cycle. Therefore, H+ exudation is a critical plant strategy for nutrient acquisition and management, allowing plants to improve their access to essential elements from the soil. A lack of water splitting during photosynthesis can affect iron uptake because the resulting energy imbalance disrupts the plant's ability to produce ATP and NADPH, which are crucial for overall photosynthetic energy conversion and can trigger a deficiency in iron homeostasis pathways. While photosynthesis uses hydrogen ions produced from water splitting for the Calvin cycle, not to create a hydrogen gas deficiency, the overall process is sensitive to nutrient availability, and iron is essential for chloroplast function. In photosynthesis, water is split to provide electrons to replace those lost in Photosystem II, which is triggered by light absorption. These electrons then travel along a transport chain to generate ATP (energy currency) and NADPH (reducing power). Carbon Fixation: The generated ATP and NADPH are then used to convert carbon dioxide into carbohydrates in the Calvin cycle. Impaired water splitting (via water in or out) breaks the chain reaction of photosynthesis. This leads to an imbalance in ATP and NADPH levels, which disrupts the Calvin cycle and overall energy production in the plant. Plants require a sufficient supply of essential mineral elements like iron for photosynthesis. Iron is vital for chlorophyll formation and plays a crucial role in electron transport within the chloroplasts. The complex relationship between nutrient status and photosynthesis is evident when iron deficiency can be reverted by depleting other micronutrients like manganese. This highlights how nutrient homeostasis influences photosynthetic function. A lack of adequate energy and reducing power from photosynthesis, which is directly linked to water splitting, can trigger complex adaptive responses in the plant's iron uptake and distribution systems. Plants possess receptors called transceptors that can directly detect specific nutrient concentrations in the soil or within the plant's tissues. These receptors trigger signaling pathways, sometimes involving calcium influx or changes in protein complex activity, that then influence nutrient uptake by the roots. Plants use this information to make long-term adjustments, such as Increasing root biomass to explore more soil for nutrients. Modifying metabolic pathways to make better use of available resources. Adjusting the rate of nutrient transport into the roots. That's why I keep a high EC. Abundance resonates Abundance.

Today 23/07/22 We are two days into week 5, never talked about nutrient or water’ problems I had some leaves with few yellow marks but very small one beside that I always saw my plants green and happy, here you can see the scrog I set up yesterday not sure if this will work better but I think will help the branches grow further from the others increasing the light they riceve! I made it myself I will order one for the next grown when I will get my grow box! The watermelon is already flowering but the northern light no so today I started feeding the plants with different preparations.. I kept on giving the grow booster with a bit of bloom booster and viceversa for the watermelon! If you have any tips anything you think I’m doing wrong or you see it’s not ok let me know I don’t grow in a box I grow between the curtains and my window so the environment wasn’t always the perfect one

This one is for you Apollo @sweetseeds Check it out! Thanks for all my brother Peace and good vibes Til 127 Days and ahead after partially harvest Will show you guys soon Stay tuned

Last straight before flushing this run was very pleasant and relatively easy I feasted on this session On these different tests I learned a lot about cars Among other things that the LST is essential and that the different apical or other sizes are to be avoided because their predefined life cycles do not allow them and this generates a loss in performance To return to my five damsels The northen light auto at @RQS was the fastest the first amber trichomes appeared at day 56 I did not expect taken from court I start rinsing The bubblegum from 00seeds still has not bloomed I plunged into the dark 20h .... The blueberry auto # 1 of pevseeds is simply gorgeous rugged and will surely give me a nice harvest it has suffered pinch of Apex and LST The gorilla glue from @fast_buds is not left smaller than the bonsai style but very well trimmed she suffered LST Apex pinch and size of the main apex ... Et en français Dernière ligne droite avant le rinçage ce run à étais très plaisant et relativement facile Je me suis régalé sur cette session Sur ces différents test j'ai beaucoup appris sur les autos Entre autre que le LST est primordial et que les différentes tailles apicale ou autre sont à proscrire car leur cycles de vie prédéfinie ne leur permettent pas et cela génère une perte au niveau du rendement Pour revenir à mes 5 demoiselles La northen light auto de chez @RQS à étais la plus rapide les premiers trichomes ambrés sont apparus au jour 56 je ne m'y attendais pas pris de cour je commence son rinçage La bubblegum de chez 00seeds n'a toujours pas fleuri je l'ai plongé dans le noir 20h .... La blueberry auto#1 de pevseeds est tout simplement magnifique robuste et va surrement m'offrir une belle récolte elle a subis pincage d'Apex et LST La gorilla glue de chez @fast_buds est pas en reste plus petite du style bonsaï mais très bien garnie elle a subis LST pincage d'Apex et taille de l'apex principal La blueberry auto#2 de @pevseeds Est pas mal mais on voit nettement que le main lining subis la stréssée et par l'occasion perdre de sons rendement final je souligne la robustesse de cette variété Merci à tous d'être passé pas ici Que la force soit avec vous !

They seem to have stopped stretching and are now focusing on the buds. Very odd coloration happening on the leaves of the left plant. I'm thinking it's either a calcium deficiency or a manganese deficiency. Either way the buds are developing nicely still. Right is looking great right now, save for a few spider mites I had to squish earlier last week. Nice and frosty at 5th week of flower. If all goes well with both of these girls, I'll be harvesting them at the end of January. I'll definitely give them a bud washing b/c I found mites. Update 1/2/22: okay I've got a few notes to make on the progress as a whole. So after topping right, it seems like she started producing flowering hormones right after. While left focused on stretching for like a week more. Right has fat, compact and frosty buds- shaped like golf balls. Left has spire-shaped buds, not AS dense(still dense though, mind you), and less frost. So I'm thinking left is staggered behind right by about a week. Since this is week 5, we'll be able to tell just how far ahead she is by week 8.

The ladies are opening up nicely after 48hs of planted (2 days) as normal, the pheno #1 has opened first let's see how they keep developing!

I let her go up to level her colas

Stretch de 7cm temperature variable 14-28degres le stretch n’est pas fini !! La Flo avance tout doucement je sent que sa va être un monstre 🤞

Finally, harvest was 3 weeks flushing without nuts am trying to test something I will see soon. Btw can't wait to smoke it 😎

Rolling into week 7. Time to dial down the nutes and start my UVB regiment. I use Reptisun 10.0 bulbs due to LED UV's giving far less than optimal performance. Reptisun 5.0 bulbs work 2. I only run them from week 7 to flush and for only 3 hours a day within the current light cycle. Update day 45: After doing a trichome check, it finished EARLY!! Time to do a quick flush then harvest. I was going to add the nutes to the fresh water today 😂😂

Let´s start Day 43 😎 some sun and cloudy with rain again 😳 Day44 cloudy and rain and cold 😡

Midnight is a seriously beautiful cultivar and unlike many other purple strains I’ve seen, grown and smoked, this one’s terps are actually rather impressive. Her aroma is a pungent mix of berries, citrus and gas. I love it. She certainly isn’t the fastest auto. We’re at day 77 since sprout and it’s clear she still needs another 2-2.5 weeks. I expect her to be ready for harvest somewhere in the 90-95 day range.

Esta por empezar el engorde final. Ya las flores estan casi formadas. La altura está al límite total. Uno o dos centímetros más y estaría en problemas de verdad. El engorde de muchas flores ya es notorio, pero creo que el uso de un bioestimulante que no es de primera línea, ha reducido el potencial de las plantas. De todos modos hasta no tener el resultado final no puedo afirmar nada. La solución de esta semana fue cargada con más pk booster para ver cómo funciona la linea de greenhouse en esta etapa. Suelo usar pk 13/15 de canna. Pero esta vez puse el que tenía. Seguiré actualizando. Día 31. Se nota mucho el engorde. Las hojas muestran pequeños signos de sobre fertilización, pero nada grave que no haya visto antes. Me suele pasar en esta semana en todos los cultivos en hidroponia. Pero las veces que ko me pasó, no tuve el engorde deseado. Por eso manejo mediciones de PPM superiores 1000. Se empieza a ver cuáles van a ser los cogollos más grandes, pero sigo con incertidumbre sobre cuanto más van a engordar. Estoy bastante conforme al momento. Hoy tuve contacto con plantas macho, pero me cambié toda la ropa antes de acercarme al indoor y le lavé. Además no sé si sumará, pero me rocío en alcohol cada vez que entro a casa, así que espero no tener problemas. Ya voy a ir preparando los esquejes para la próxima siembra. Quizá no logre sacar 10 de las madres que tengo. Pero quizá ponga menos y los haga crecer más tiempo y con bastante LST. Pero será en otro protecto. Por ahora espero que siga el engorde. Día 33. Ya el engorde es un hecho. Por el momento estoy bastante satisfecho. La solución se concentró demasiado así que tuve que agregar agua. Pero más allá de algunos signos de sobre fertilización mínimos y pasajeros en las hojas, la plantas se muestran saludables y firmes. Todavía sigo buscando la posibilidad de agregar overdrive la semana que viene. Todo está por verse. Ya puedo asegurar que va a haber una séptima semana de floración, sino debería empezar el lavado en 2 días, cosa que a la vista, sería muy apresurado. Así que seguiré esperando a ver las flores con los pistilos más secos. Entre tanto los tricomas ya están formados, pero recién van a empezar la maduración en unos días. No saco foto porque el celular no hace buen foco con la lupa, pero ya intentaré mas adelante. Día 35. Cierra la semana con una buena idea de lo que van a ser los cogollos. Según el calendario de la planta, debería empezar el lavado esta semana, ya que sería la última. Pero por la madurez de los tricomas, todavía le faltan entre 10 días y 2 semanas más. Así que se viene otra semana de sobrecarga de PK y voy a aumentar la dosis para aprovechar a medir el nivel de tolerancia de la planta a altos niveles producto. Al ser hidroponia y trabajar con el ambiente bastante controlado, confío en que voy a poder aplicar correctivos a tiempo, si empiezo a notar defasajes bruscos.

Empieza el adiestramiento! Corte apical en el brote central y defoliacion general😋

Day 43. Light is on max output, heat enormous and they RUN !!!! thought intensity will stop them a bit , but i think i do only worse ... We have +30 heatwave in UK, i live in attic , so for 4 more days everything will be out of control. Watered today. 6.3 phed water. I love simple and affordable Mars Hydro products, if you can cope with heat TSL2000 can do magic in your tent ! Will update after heat wave us over. Should be in 4 days back to +20 ;))) Day 45. They GROW !!!! Distance is insanly small, but i have 30 cm of space left, wont move light for a week, then i will try to have 20 cm at least again. Planing last top up, need two more waterings before it, so it should be on last day of this or first of next week. Thinking to take down all LST at that time, need pots to breath better, too thin fabric, they dont keep form. Day 47. Drink every two days !!! 4 liters goes to nothing !!! Huge, still streching, tops almost rubbing TS2000, heat 30 inside, humidity 65-68 ..... will need heavy clearing again !!! Devil is looking droopy because was just watered. Happy Growing !!!!

Up and running, everything smooth, growing a lot. The lesson I got is that my space is just too small for 3 plants in 5 gal smart pots... will update the space soon and invest in a LED. Started giving some TOP CANDY from Topcrop fertilizers, smells sweet as molasses...

Aun no e terminado con ellas pero para ser mi primer scrog lo veo bien o que me decís vosotros?

Taken down today. Chop,dry,burp,cure to follow. Good season, no mold, no bugs, frosty nugs. Will smoke the entire yield in one video take on harvest week. Stay tuned