All content on this diary is for inspirational and educational purposes only. The ideas shared are not a substitute for professional advice. This diary/account is not officially affiliated with Alan Watts or his estate. All materials are used under the principles of fair use. I honor the legacy of Alan Watts by sharing his wisdom respectfully and with the intention of inspiring awareness and self-understanding. 2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

What’s up guys! First time growing and I will be growing an auto white widow. I started Germination 3/4/20 and dropped it in roots organic soil in a 3 gallon pot. Any recommendations on proper temperature and humidity levels for the next week or so. Along with proper water timings, I know it’s best when slightly dry but anybody got an average of hours on hand that work best? Thanks for tuning in and hope to see you guys follow along and help me grow !

The girls have survived another week ❤️ I did a light defoliation and have been monitoring the RH closely. We have had a very damp week here, and the RH kept climbing and had my fan working hard. They are starting to plump up and fill in 😋. They have also started to slow their drinking habits, instead of watering every three days, it has moved to every 4 now. Just a couple of weeks left, I think I will be harvesting these over Christmas 🎄, and will have some great bud ready for the new year! 🎉🎊🍾

Hey now, hope everyone is having a great weekend and staying safe. Another great week for the ladies, as they are now about 2 weeks into flower, and the stretch is winding down. I am still feeding them silica, cal mag, and flower nutrients maxi bloom by GHE. I will start to add a PK booster this week. I also will be switching to light from V1 to F1 sometime this week, and also integrating the UV/IR bar into the light cycle later this week. I will run the UV/IR bar for approximately 30 minutes prior to lights off to get started. The 5X5 is really starting to fill up nicely, I am running two dehumidifiers outside of the tent and I am able to maintain around 50% RH in the tent during lights on, and around 55% RH with lights off. Still very happy with the Spectrum X from Medic Grow. I am running her at 90% in the V1 cycle and will be transitioning to the F1 cycle later this week. Overall, the plants seem very happy and healthy. The temperature and humidity is significantly dropping so that helps keeping the tent cool and not overly humid. This is by far the best time of year to be flowering plants in my region. I hope everyone has a great weekend, Thanks for stopping by, Stay Safe and Blaze On!!! 💪 Website: https://medicgrow.com/ https://growdiaries.com/grower/medicgrowled

My growing boxes with Barnes Farms- Lemon Cherry Gelato,White Runtz,RS11&Banana OG and Spirit Seeds- Watermelon auto,Critical Kush auto,GG4#fem,Northern Lights Auto,GOrilla Auto Hay algunas preguntas sobre mi election del strains?)

Okay, so interesting week all around for both myself and this plant. First off, apologies for the limited pics this week. A lot of time was spent on another plant, but going forward, you should see an improvement. Second off, more apologies. This time, for the time-lapse video. It appears a bit jumpy during the first period as I was getting it properly configured, before bumping it out of position. I also realized I could zoom in with it. Now, on to Pink Lemonade. Its been an interesting start with this lass. Possibly I moved her over a hair early to her final home, or I somehow caused her some stress, but the week started off slow. But that all changed as the week went on, and she quickly produced her 2nd, and now 3rd nodes. From what I have seen, this plant, at least thus far, seems to stress easily, but once she comes back, she comes back with a vengeance. For those wondering what my intents with this young lady are, I plan to top her at least once, possibly twice, but no more than that. This grow is more about quality than quantity, and even though I should not lose quality by having more, I am also attempted to have this completed by a specific date. I would like to be harvesting this young one for beginning of sept. As per the seed company, she has a flower time of 8 - 8.5 weeks. Pretty tight time frame. We will find out how accurate it is. Pretty much 2 months exactly. That leaves me approximately 10 - 11 weeks to work with the plant, but as I have never grown this strain, I may move her to the bloom tent sooner. Also dependent on how fast she grows upwards as well. Until next week, keep on growing.

Esto está Explotando!! MADRE MÍA LA CANADIAN KUSH de Medical seeds!!! BRUTAL!! 🍁🇨🇦

Terza settimana di fioritura inoltrata..andiamo più che bene con lo sviluppo.buona genetica anche questa papaia cookie...bravi 👏 fast buds. Continuate così .,💪✌️

Vean el video que comenten porque se secaron las hojas???

Our beauty is now 3 weeks old and has grown to nearly 18 cm tall, continuing to develop strong and healthy. We’ve decided to enhance her nutrition by continuing to feed her with Xpert Nutrients fertilizers, which provide everything she needs for balanced growth. Additionally, we’ve started using CO2 to further stimulate active growth. The daytime temperature remains at 28°C, nighttime at 21°C, and the humidity is maintained at 65%. We are still keeping the light schedule at 12/12, closely monitoring how the plant responds to these conditions. A big thank you to Xpert Nutrients for their high-quality fertilizers, and our CO2 experiment promises to be exciting!

Woche sieben beginnt ganz wunderbar mit Wunderschönen roten Blüten und einem hervorragenden Duft. Sehr beerig, süß nach Kaugummi. Ich liebe diesen Strain jetzt schon❤️ Am Sonntag gab es noch einmal den Enhancer von Greenhous feeding. Jetz noch eine Woche Wasser und dann gibt es in der letzten Woche nur noch Licht, Luft und liebe 🏻

Weather is better now. Slug Population ist decreasing.

Die Woche nur gegossen und Lampe hochgemacht sonst nichts sie ist schon sehr harzig bin gespannt mal gucken ob sie lila wird müsste ja eig bei granddaddy purple

Beans keeps trying to let me die but I'm not going out like that. Hes probably just jealous of my reproductive organs📏

Letzte Woche?🏽

Verdacht auf Zwitter und ein paar Nenas gefunden die wurden mit Pinzette entfernt

The bananas are quite progressive, they were like this from the start, they are one node ahead of their sisters. Planning to top them at the 6th node this time and give around 5-6 weeks of veg.

Hey now, hope everyone is having a great weekend! The Strawberry Banana Auto from Fastbuds is starting to smell very good and starting to stack up nicely. She is only 26 inches tall but I feel like her colas are going to be quite thick. I did a light defoliation this morning and took off all the LST tie downs. She is looking super healthy and loving this Medic Grow Spectrum X. I currently have the Spectrum X running at 100% power and also integrating the UV/IR bars for 75 minutes just prior to lights off. I am super happy with this light so far, she puts out impressive PAR, and runs fairly cool. The only thing I would change is to add a timer just for the UV/IR bars, and allow the main light to be run on a timer. I will probably do one last light defoliation in the next couple of days and that will be about it other than keeping the reservoir full and in the correct PH range. The tomatoes outside are absolutely loving the runoff from these autos, as that is all they have had to drink since they were about 3 weeks old. Thanks for stopping by, Stay Safe and Blaze On!!! 💪 Website: https://medicgrow.com/ https://growdiaries.com/grower/medicgrowled

.