2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Started Training Late! Was on summer holiday for a week and only started training now. Above are the plants during training as well as a few hours after training. Autos are definitely more delicate and the two runts are growing slow but yet still showing some progress. Changed my bulbs back to the CMH set to 400w each (2) Also gave them a good amount of water as the FloraCaps aren’t covering the whole pot I felt like it was necessary to wet the entire grow medium well for a change. Some of them were a bit far along for training but I did the best I could. Also the Purple Lemonade and 2 other plants are showing signs of flowering.

This is what's left in the tent, the gelato is comin down tonight and maybe the 2 smaller lemon aks maybe tmm. The cream cookies and other lemon AK will come down soon too and then I can get some pics. The pineapple and blue dream get 1 more flush

Aquí está la pequeña banana tirando como puede de manera que pelea por el espacio de iluminación contra las amnesia. Una locura este híbrido entre tantas sativas! E de decir que me gusta 😊 Seguimos subiendo poco a poco la ec este riego en el día de hoy 30/3/26 le e metido un pequeño chute de 70l con un ph6,4 Estamos alimentando a la pequeña con 1800ec Y parece aceptarlos bastante bien!!

I actually harvested Sunday, Tuesday, and Thursday. First harvest was tallest buds only, they were probably 30% amber. Second harvest was the rest of the upper canopy three days later. Third harvest was the lowest buds.

Per ora tutto bene . Haze berry in fiore 👌

Que pasa familia, vamos con la cuarta semana de vida de estas Gomu Gomu Mango feminizadas de HighBreed. Vamos al lío ,se colocaron en macetas de 7 litros definitivamente. El ph se controla en 6.0 , la temperatura la tenemos entre 24/20 grados y la humedad ronda el 50%. El ciclo de crecimiento puse 16h de luz, el foco está al 50% de potencia. De momento va creciendo, tiene buen color y va progresando. Gracias a Agrobeta por el kit para la temporada, unos jefes, siempre apoyando 💪. - os dejo por aquí un CÓDIGO: Eldruida Descuento para la tienda de MARS HYDRO. https://www.mars-hydro.com Hasta aquí todo, Buenos humos 💨💨💨

Stopped feeding this week other than one watering with molasses. Needing to be watered every 3 days. Other waterings using ph'ed filtered water to 6.5 set on my air bubbler for a few hours before watering. She's very close to being ready. Another week and i think she will be perfect. Notice leaves fading end of last week and they have been getting more color throughout the week. Bud growth only happening closes to the light and much of orange hairs are white. Not a ton of amber trichomes yet which is good. Really liking how this plant is turning out. Nice loud terpene profile and fat dense frosty buds. Trimming should be pretty easy on this one which is a relief knowing how much work I have ahead of me. I think this very could be the fast version.

7/1

☘️El 21/04 puse a hidratar la semilla por 24hs y luego la coloqué en papel con un poco de agua para empezar con su germinacion. ☘️22/04 - 24hs después germino y midió 1cm. ☘️Coloque la semilla germinada en una maceta que abarca 3L (la marca que utilizo en macetas es "Roots House"), es momentaño ese tamaño ya que me quede sin tierra suficiente para llenar una maceta de 10L como de costumbre. ☘️Le agregue AEGIS que es un producto natural compuesto por propagulos de hongos formadores de micorrizas. Estos se presentan como raicillas colonizadas del hongo y esporas. ☘️El sustrato que utilizó se llama "Nube Verde", su composición es de turba de categoría, perlita, humus, compost, tierra, dolomita y verticalidad. ☘️Voy a dejarla dos semanas en etapa de enraizamiento para que se pueda desarrollar de la forma correcta así la puedo pasar a crecimiento. ☘️25/04 - Cumple cuatro días de vida en total, ya mide 2,5cm de alto. ☘️En estos dias la estaré trasplantado en una maceta de 10L ya que ahora se encuentra en una de 3L. ☘️Salió muy rápido, por el momento viene de manera correcta. ☘️En estos dias estaré subiendo más imágenes! Podes seguirme en insta gram como @bruweed_arg !😶‍🌫️

Sie schauen sehr gesund aus oder was meint ihr

Tardamos un poco en finalizar la anterior y además con alguna que otra plaga, estás se están recuperando de las plagas bastante favorablemente... esperaremos con el transplante coja más cuerpo farmers!🙏🍁

1/12: impatiently waiting for lemon auto to finish up. Still not sure what the northern lights is trying to do, but..the little buds are slowly growing. S l o w l y... 1/14: things are getting out of hand in here. Northern lights is bushy AF again but the budlets appear to be increasing in size. Lemon is getting closer every day. Looks like I've got milky trichomes and the pistils are darkening. Still a bit early, but I'd be lying if I wasn't anxious for the space.. 1/15: I've accepted that I have at least a week ish left on Lemon auto,but she's getting there! I did some rather risky defoliation on northern lights and holy shit, she's got potential. I'm hoping she's just a slow grower and those buds will really fatten up! Perhaps lemon is just a really fast growing plant? I've also decided that since space is limited, my best bet is to probably rotate the plants every other day to allow all bid sites an opportunity for direct light. I'm reigning myself in. Once lemon auto is harvested I need to NOT immediately plant another. I've got some mephosito seeds I want to get going, but I've learned my lesson. This tent fits two 5 gallon mature plants... Uncomfortably. Three is too many 😂 1/17: wondering if northern lights has is it her to fatten up. Gave her a good flush &feed. Popcorn buds or not, I'm invested (#forscience). Lemon auto is still finishing up. Can't get good enough pics with phone to see trichomes. Clearly, I have a minimum of a week left, but I got a digital microscope so I can keep track! Should be here for next update.

🗓️ 9° WEEK FLO // DAY 57-63 (from switch) // DAY 141-147 (from the sprout) ⚡ Light: 40 cm / 200 watt. Due the high temperature, I set my controller on 29° C max. So the Tent-X dimmed the light to keep my grow-box in the right range; ⌛ Schedule: 12/12; 🌡️ 29.5° C - 65% RH average; 📑 PH 5.7 - EC 1.8; 🌱 Trichomes mostly cloudy, ripening blend is working well; ⚙️ Fans, extractor and pump ON 24/0. No dehumidifier.

After a bit of a leaf defoliation on day 34 of flower girls did not stress at all. flowers are hard and dense not fluffy at all even in the middle of trellis the buds are hard .now for week 6/7 I will cut out the magnifical from there I will be heading to week 8 using just ph'd water and nature's candy and week 9/10 for the final flush I will use just ph'd water

Only issue was I didn't grow enough!