2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

The northern lights from herbies was a best the plant was the fastest growing plant out of 10 strains. In the same amount of time as 9 other plants of different strains this plant grew huge 2 times as big and it finished up flowering after only 45 or 46 days of 12/12. It has a good smell. I only set back is it doesn’t seem to be super dense with tricombs. Don’t get me wrong the plant has plenty I would guess around the low 20% of thc. That’s not bad at all it’s just not a 30% plus thc type of plant but it’s still a great plant

Chopped on day 82, and dried for 8 days. Produced 27.9 grams (1 Oz) of flower. The yield isn't great, but it's my fault due to stressing and transplanting the plant. I had to fix her and by time she was back on track, the yield was severely impacted. However, the quality seems great. Now time for curing.

Small plant, but fat top, so 20-ish grams it looks like 👍 can't wait!! ♥️

Week 19, day 6. Second week after switching to 12/12 lighting. Nr 1 is 155 cm tall from top of soil and Nr 2 is 171 cm. All video and pics is taken when the girls have hade 11.5 h of no lights and just got watered. The stretch on these girls was incredible the past 2 weeks after switching to 12/12 lighting. So fare this has gone smooth as silk and no problems at all despite temperatures over 30 degrees Celsius for the last 45 days. I have manage the heat by having between 85-95 % of RH in the room all the time. And this genetics from DinaFem seeds is awesome, i hope i will get a massive harvest of strong indica medicine.

I transplanted the plants to 23 liter containers and added 2 extra Guana Flowering Tabs 😇and Flower Safer (mycorrhiza) I gave the plants a liquid booster with OPF 7:2:3 ; Fulvic 25 ; Yuccah ; Biovin liquid ; Earthworm manure extract. Big Thanks to Luc from Plant Health Solutions / Bioplantenvoeding dot nl 🙏🙏🙏

Damn 1 of my Grand daddy Purp plants is growing like its life is depending on it lol. As you can see I swapped out that cob light and added my SE-3000 to the room so that I'm running all Spider-Farmer lights during flower. I put it over that tall GDP because I can let the plant get a lot closer to ot then one of my SF lights. I have to say when it comes to what I consider Amazon Special grow lights (Mars Hydro, Phlizon, Spider-Farmer, Viparspectrum, Etc) Spider-Farmer is by far the best. The SF-4000 light I have is still running at 100% after using it for roughly 10 grows now. Other LED brands I've ran only lasted 2-3 grows before they needed to be replaced. I've had the SF-2000 for just over a year now and it's got about 4 grows on it and again still working 100%. Now I just finished my first run in my 3x3 with the SE-3000 (last 2 weeks I'm running a blurpe LED light to see how it effects the potency) and I was extremely happy with thw outcome of the light. For only 300w I feel like it did a better job then my 450w SF-4000 light in my 4x4 tents. The humidity levels and temperature have been doing great all week during day/night even with temperatures outside dropping to 3c. I'm expecting November/December/January to be very difficult out here to try and keep the temp up. So what I might do is run a 400W HPS light in my 3x3 next go around during the day to keep it warm and then run the main room at night with the LEDs. We'll see what it's like when I get there tho. Also thinking of moving the 2x2x4 to the main room and putting my 2x3x5 tent were it is. Again tho we well see. Well that's all for now. So happy growing everyone!!!

The Dr seedsman CBD germinated backwards but she looks fine🤞😅

They continue yellowing and I have raised the concern in questions. People say it may be natural cycle ending , but northern lights seem to have some more time to go. I fed them EC 1,3-1,4 a day before posting. I will stop watering every day , seems like even low nutrient solution lead to build up , even though I did flush. Doing nutes-water -nutes till the end flush. Fellows, please also let me know , is this result fine , considering the fact I was dragging them with 300W this whole time and installed the 600w light a week ago ?

Everything good for now,plant reacts good to nutrients 😍 Continuing with bit of lst.

Note : Jegliches Equipment aufgelistet welches ich nutze findet Ihr in der Germinations Woche !!! Day 15 : ich weiss noch nicht ob der mineralische dünger wirklich sofort etwas gebracht hat . schauen wir was die zeitraffer so vermitteln. ist es seit gestern schneller am wachsen ? Day 16 : PPFD 275 * 20h = DLI 19.8 Day 17: + 0.5L Flaschenwasser (EC 0.26) + + Canna Rizothonic + + Canna Terrar Vega + = Total EC 0.68 (PH 7.2) + Final PH 6.15 Day 18: es wächst Day 19: es wächst immer noch ventilation übern blätterdach angemacht. ich glaub daher kommt das ruckeln im video Day 20: + 0.5L Flaschenwasser (EC 0.26) + + Canna Rizothonic + + Canna Terrar Vega + = Total EC 0.68 (PH 7.2) + Final PH 6.15 + 0.5L Leitungswasser (PH 8.0) Der Stamm hat sich in den letzten 3 Tagen verdreifacht vom Volumen. Jetzt hat er so Bleistift dicke erreicht. Day 21: die temps und relative luftfeuchte sind hier zwischen 58%-68% und 23.5°-25.5°C . reguliert sich aber immer zu den höheren werten . ist hier halt so :))

We didn't have much hope for this one, hence the light documentation... her PH was a rollercoaster for a while but still managed to yield around an oz..not too shabby for a plant we nearly tossed... if we grow this strain again it will most certainly be grown outside in the garden.. I believe this could of been a very large auto given optimal conditions...happy growing everyone!

Week 11 April 23 - stretch seems to be over and I’ve raised the light to max height so let’s hope -looking good so far no deficiencies other than a bit of light burn/bleach because lights were to close -will be top dressing later this week before a watering -no smells just yet and thankfully as I need to get the temp down in the tent before later in flower

Всем привет друзья! Кустик готовится к харву. Очень приятно начал пахнуть. Мне кажется, что он начал меньше кушать. Всем добра и хорошего роста!

shortest plant is 50cm tallest being 95cm. They are all doing wonderfully, most of autos are budding nicely and all of the photoperiods are ready to flower. looking very promising. i am changing my light schedule to 14-10 to kick these girls into flower.. this will give the plants an extra 17% (or so) a day under the lights.rather than 12-12. i cant be fussy as i only have one grow space but if my research is correct these should grow beautifully! the autos are well ahead so shouldnt affect my harvest weight too much. like i say i cant be fussy! thanks for looking

------SETUP------ -HOMEBOX TRIANGLE+ .4sqm -BOSSLED LIGHTS 250W REAL -INTAKE FAN HIGH PRO - 107 m³/h -EXHAUST FAN PROFAN - 220 m³/h -TWO SMALL VENT FANS -HUMIDIFYER WHEN NEEDED -AC IN THE ROOM -15L SMARTPOTS -50%PERLITE 50%SPHAGNUM PEAT MOSS ------STRAIN------ SEEDSMAN BLUEBERRY = SM BB. Blueberry was originally created by Canadian DJ Short and dates back to the 1970s. It is a 3-way indica-dominant hybrid cross of an Afghani strain together with Thai and a Purple Thai. When grown in good conditions it can grow into a fairly large plant displaying a range of red, purple and blue colours and producing very good yields. Another quality of Blueberry is that, although taking a while to cure out, when correctly prepared and packaged it has a very long shelf-life compared with many strains available today.. /BOUQUET Unsurprisingly when fully-cured Blueberry has an incredibly fruity perfume along with the unmistakable sweet flavour of blueberry. /EFFECTS The effect is a pot smokers dream - a very potent yet happy high which is very euphoric yet relaxing and enduring. ------GENERAL COMMENT------ The first 7 days of this grow is almost complete, Blueberry and the AK47 germinated fine, had some issues with the Candy Kush Express, so the last seed is germinating, and seams that it will emerge soon! I'm optimistic, so basically till now we will do a free seed run, since this ones came with the genetics I purchased from seedsman and gyo green. 😎😛 -251217 First feed with a very light nutrient dose. Will implement some cal mag next water. Humidity has been a little hard to control, is oscillating a lot. ------SM.BLUEBERRY COMMENT------ Development is ok, nothing overwhelming for now, lets see how things evolve. Still not convinced of the easy plugs, kind of hate them, direct soil seams more effective. 👍