Der Topf ist unten geöffnet die Wurzel kann sich entfalten. Nach Oben ist nicht so viel Platz. Die Pflanze zeigt sehr interessante ,, Albino" Ansicht. Dünger wird verlangt. Blätter zeigten Magnesiummangel, mehr als gewöhnlich (2x in 4 Wochen) zugefügt.

Very strong bud growth this week! I have 1 out of the five that its quite a bit ahead of the others, I will post a photo of her main cola. Otherwise, they are looking fantastic. Smelling amazing also. Today is day 77. So the end of week 11. Looking forward to going to space on these mango beauty's! Introduced them to black strap molasses tonight in their feeding. Ph water to 6.2 with 5ml of mollases per gallon. This will be my first go with mollases, hoping it helps build bud density!

After flushing them and defoliation, they have come back looking much more bushy! I’m definitely happy with how they are coming along, though would like to see some more stretching and growth from the smaller plant. They had their first water in 9 days on the 11th

Thursday 6th August (day 40) Bad feed had high run off numbers again and progressing tip burn flowers developing slowly. This is all down to environment and my mistakes, Im having an uphill battle correcting it and its tiring. I keep switching my strategy to try and get a consistent approach 0.5ml/L - Nirvana 2ml/L - Canna A&B 1.5ml/L - Big Bud 2ml/L - Big Fruits PH5.8 1.3 Friday 7th August (day 41) Last ditch effort now, I’m going to continue feeding this will increase frequency over the next 2 weeks. 2ml/L - Organic Bloom Liquid 2ml/L - Big Fruits 2ml/L - Clean Fruits 0.5ml/L - Big Bud PH5.8 EC0.6 Saturday 8th August (day 42) 3-6am Took some leaves off both plants, the ones that were touching buds and pointing inside the plant, for circulation and reduce some humidity. 2ml/L - Organic Bloom Liquid 2ml/L - Big Fruits 2ml/L - Clean Fruits 0.5ml/L - Growzyme PH5.8 EC0.5 1st feed 3L 300ml run off =10% slow pour Will make another batch so I can get some more run off same feed again. Run off BBM PH6.5 EC0.9 Run off Cheese PH6.3 EC0.9 Inoculated with 1g Living organics to 4L 2ml/L - Organic Bloom Liquid 2ml/L - Big Fruits 2ml/L - Clean Fruits 0.5ml/L - Big Bud PH5.8 EC0.7 Fed this in small amounts removing run off up until 3 hours before lights went off. I have 2 weeks off work now so I can try and keep the environment in check and increase my feed frequency I will do 2 feeds a day 6 hours apart for the next 10-14 days Monday 10th August (day 44 FFS!!!!! I just found out my lights have been on 24/0 since I changed the timer 3 days ago!! Opened tent to find a lovely bunch of BANANAS looking at me… Plucked a few of them just going to leave them and pluck the yellow ones going forward. 1ml/L - Organic Bloom Liquid 2ml/L - More PK 2ml/L - Big Fruits 2ml/L - Clean Fruits 3ml/L - growzyme PH5.9 EC0.7 Didnt it have to Ph adjust More Pk lowers my PH

Alles wächst schön gleichmäßig und alle 3 Strecken sich gut. Die Chem Cake etwas mehr als die anderen zwei, wurde ja aber auch nicht gestoppt. Tagsüber erreichen wir jetzt 25° C und das ist ne super Temperatur, RLF war etwas niedrig, aber die Töpfe waren auch komplett trocken. Jede Pflanze hat ca 5 L bekommen, das bisschen Drain hat die Wanne nicht annähernd gefüllt und das sollte dann die RLF auch wieder etwas anheben.

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Day 60 today and 5 flower week 💪🏻

Week 1 germination 100% 1 shot glass of water was maker sure the soil was damp with sprayed around soil

PPD has the whole room smelling amazing. Good stacking is underway and the terpenes are so loud and fruity already it's awesome. 120 watts of undercanopy has brightened it up for sure and the low bud sites have responded with increased pistil development. Let's stack it up!

amazing growth this week from all 3 plants. The peanut butter cookies is recovering from the transplant. Both the auto cheese and auto cinderella jack have both started to show signs of flowering and have bushed out incredibly well. The pbc has had its first top done and has started growing 2 extra nodes. 07/08/2020 - training has been done on all the autos. They have recovered extremely well and seem to not be slowing down. Finally starting to see the type of flowers I have been striving for. After a semi unsuccessful first grow, this has been a huge relief. Both autos are now showing signs of going into flower so I will try to stop LST unless any unruly branches form. The peanut butter cookies has now started to fully recover from the transplant and topping. Will now allow this to flourish and grow as big as it wants as both autos should be done before its too big (hopefully)

EC 1.2 PH 5.8 I am very impressed by the rapid growth. The roots seem to be very healthy. I have raised the light to encourage more vertical growth.

Last week the plants grew looking very healthy although I still have a feeling the low humidity is slowing their growth, by the end of the first week I started raising the EC to make the increasing for this week smoother, run-off EC is substantially increasing so I will start to fertigate them twice a day for this week.

An in to soil she and her sister testers go, i know that for know everything looks the same , apart from the photos, but this will start changing with them growing. For now all of them are growing pretty much the same and now that they move in to soil and start feedings they will start showing the phenotype and genetic differences, but for now all looking great , healthy and strong af!!! Haded a intro video from seed in to now, including how i transplant and what i use wen doing it so, and from here the moon is no limit for this babes i believe , so stay tuned as this testers they promise to be a interesting ride <3 <3 <3 They grew amazing for only a week fast and vigorous, all the testers for now look very the same <3 <3 <3 As always thank you all for stoping by and for supporting me on this journey, i am super passion about growing and fell blessed to have you all with me on this new journey <3 <3 <3 Genetics - Fast Buds Tester 08 09 10 11 Ligth - LUMATEK ZEUS 465 COMPACT PRO 
Food - APTUS HOLLAND #aptus #aptusplanttech #aptusgang #aptusfamily #aptustrueplantscience #inbalancewithnature #trueplantscience #fastbuds #dogdoctorofficial #growerslove With true love comes happiness <3 <3 <3 Always believe in your self and always do things expecting nothing and with an open heart , be a giver and the universe will give back to you in ways you could not even imagine so <3 <3 <3 
All info and full product details can be find in can find @ https://2fast4buds.com/ wen released 

https://aptus-holland.com/
 
https://autopot.co.uk/ 

https://lumatek-lighting.com/ <3 <3 <3 Growers love to you all <3 <3 <3

Hey everyone 😃 The time has come , On flowering day 69 the harvest was carried out 😍. After spending two days in the darkroom at 62% humidity, they were now harvested cleanly by hand 😃. After harvesting, they were laid out on the net as usual, where they can now slowly dry again for about 7-10 days at 62%. Then they come with 62% in jars for 2-4 weeks, and then the 58% boveda packs are put in 👍. Of course, I will come to the phenotypes themselves in detail in the last update. Until then, I wish you all the best, stay healthy 🙏🏻 and let it grow 🍀👋 You can buy this Strain at https://www.amsterdamgenetics.com/product/kosher-tangie-kush/ You can buy this Nutrients at https://greenbuzzliquids.com/ Type: Kosher Tangie Kush ☝️🏼 Genetics: Kosher Kush X Tangie 👍 Vega lamp: 2 x Todogrow Led Quantum Board 100 W 💡 Bloom Lamp : 2 x Todogrow Led Cxb 3590 COB 3500 K 205W 💡💡☝️🏼 Soil : Canna Coco Professional + ☝️🏼 Fertilizer: Green Buzz Liquids : Organic Grow Liquid Organic Bloom Liquid Organic more PK More Roots Fast Buds Humic Acid Plus Growzyme Big Fruits Clean Fruits Cal / Mag Organic Ph - Pulver ☝️🏼🌱 Water: Osmosis water mixed with normal water (24 hours stale that the chlorine evaporates) to 0.2 - 0.4 EC. Add Cal / Mag 2 ml per l water every 2 waterings . Ph with Organic Ph - Pulver to 5.8 .

First week of flower, going well in the SCROG. Nothing fancy, just a 4x4 AC Infinity trellis. Will add a custom 2nd trellis if needed. She stunted in veg due to a bit of overcrowding causing less than ideal environment in her spot, but I fixed that by raising her, creating more space for her, and light defoliating. Other strains in this grow- Skywalker OG, Bruce Banner, Apple Betty, and Runtz. Check out my other diaries for those.

After a rough overwatering, i defoliated a ton of curled leaves, she is currently still recovering (defol and over water 2/3 days ago) Honestly though... The smell is something else, super pungent gym socks + mangoey ferment + hash Pistils starting to brown out so im guessing maybe 1/2 weeks from now til finish!

So all the plants are topped twice now and have all took well , my next step will be lowering my scrog net into position and also attach some gutter for collecting my runoff