All photos are from the last day of week 3. This week we had some problems with the temperatures, not to mention the broken fan. We solved the problem by removing the power supply from the Lumatek ats 300w pro lamp and taking it outside the tent. Everything seems to be fine even if there are some yellowish leaves.

They grow amazing without any problems Day 38 I switch to bloom cycle 12/12 I defoilate her next week Day 39 she is preflowering such good strain I bet Buy new light guys philzon 2020 Pro led its lovely 😍

Day 1 of flower 8/2/25

I guess the main takeaway from this week is to always have runoff when feeding with nutes and 12 inches should probably be the closest the light gets to the canopy on max power 75% strength bloom boost is probably too concentrated a dose. I fed her about 5 days ago then plain water then a molasses water. Probably getting a bit more molasses tonight then plain water til harvest which could be as soon as Monday the 22nd but will probably be a bit later. Happy growing friends 😶‍🌫️

📆 WEEK 13 🌱 Plants: The indica-looking pheno (the one on the far right) has a sweet kushy smell. The middle plant has the classic RuntZ aroma, and the plant on the far left has a distinct Gelato smell. Honestly, I wish I’d kept a clone of the one on the left instead of the indica-leaning one, but that’s just how it goes - I chose the one with better root growth, and how that’s translating to buds and flavor will be revealed soon. The one on the far left smells like a clean Dairy Queen cooler, flat Z terps, just a really clean scent. It’s wild that they all started with a rotten-lemon smell and evolved into such different profiles. Typical F1 generation behavior. 💧 Hydroponics: Full solution swap today: water came in at 6.6 pH and (yikes) 0.4 EC. Added 5 mL pH down and 8 gallons of Heavy 16 Flush Week to the 8 gallons of water (this is the one time I stick to the manufacturer schedule). Enviornment: Lights are all the way up; the tent thrives best when I can manually breathe it, otherwise it starts messing with its own humidity and CO2 levels. ⚙️ Equipment: I temporarily lost access to my online drive with the feeding schedule spreadsheet, which is way easier to digest than manually calculating feed per gallon and how many gallons I actually used (since I never feed full-strength manufacturer nutrients). This week was simple though - the buds are deep into swell, so I’ve started flushing. ⏳ Timeline & Outlook: The flush will be swapped for pure water sometime this week while the tent’s lights are OFF, which I think is a critical step. If we’re still pulling salts after the pure water flush, I’ll swap it out for fresh water based on the nighttime readings. On 4/4, I’ll swap the solution to water only at night. Then on 4/7, I’ll swap the water flush for a fresh water flush at night. The target harvest date is 4/14, depending on how the flush is going and how the foliage burn is progressing, though this could shift by a few days.

Things are moving right along. She is loving life and starting to frost up. Stacking is nice, And she smells like pungent cheese.

7/24/23 - Day 42 - Ok, so where to start? I'm going to go over the last couple days of issues/troubleshooting. We had an issue with the lights not coming on for a whole day/night cycle. That was a couple of days ago, and everything seems okay since then. I had some Canoeing/tacoing on the leaves once the lights were back on. I think I had the PH too low, 5.8. In flower, I should start moving it up the scale. Once I corrected the PH to 6.2, the canoeing/tacoing in the leaves seemed to be gone/almost gone. I'm REALLY worried about the height of the plant. She is only 12 in. from the light that I cannot move. I added a second scrogg net???? I don't know if that's done a lot, but I'm going to try and stop if from growing straight up. I think the best I can do is give it a lean. So Ill call this the ScoggGL (GangstaLean). You'll see some pictures up top as I start the ScoggGL I did the first big trim and pulled a 3-gallon bucket of fan leaves out. I cut off a few side branches I didn't want the plant to waste energy on. I trimmed so many leaves off it was crazy. The smells have been awesome! You all know the smell of the fresh plant stems and leaves as you trim and rub across them. It's one of my favorite smells, which is in full effect. I noticed that I have some pistils growing. I know what that means, shes going to have some flowers here in the next week. I couldn't be more excited!!!!! The roots are just downright ruthless; they have been growing out of control. I have a great picture of them in there with no water. I should note that I did two things that might have solved the Tacoing in the leaves. It could have been one or the other, but it also could be a combo of both things. I did the trimming to get some airflow between the leaves and changed the target for my PH from 5.8 to 6.2. When moving into flowering, I should have already done that. But I did both things while the leaves were Tacoing, and now they are not. I wanted to make sure I noted that. 7/26/23 - Day 44 - Things are looking great. No more tacoing, she's growing and still drinking about 2+ gallons a day of water. I hope the vertical growth stops soon, I want to remove a ton more fan leaves, and I'm hoping that it spends more time growing around than up, I'm keeping some leaves on just to slow some vertical growth. 7/27/23 - Day 45 - She's sleeping right now, but I will chop some more fan leaves when she wakes up. She's growing too tall, and I need to find a way to stop her. I think shocking her system will help stunt her growth. How crazy is this, what a conundrum I'm in. Normally I stress out trying to find the right time to trim to avoid stunting growth. I always keep my plants busy and do one or two chops. I think they need to grow as mother nature intended. Let them grow and do one, maybe two fan leave chops. That's always been my motto and its always worked out great for me. This is now the exact opposite. Feels weird even thinking about it, doing a leave chop two weeks into flower..... ARE YOU INSANE!!!!!! Lol The 2nd chop has happened. I took a ton of fan leaves off. It's time for the plant to slow down heal and start working on the buds! Let's see how this goes over the next couple of days. I added a timelapse of me chopping her up. 7/28/23 - Day 46 - After adding more water last night, I was checking PH, and I did a quick check on the ppm from the nutes. If it went from 600ppm down to 130ppm in a couple of days. So we decided to give her another dose of nutes so she has something to eat. I'll update you after she wakes up for the day. If you are looking for this experience, check out https://www.getleaf.co/

This week is the last week before water flush. Today I start only with the water and in a few days, I'm going to harvest. I can't wait to test the quality of the crops.

End of week the plant received flash clean and soon she will be ready for harvest.

Sorry guy I’ve been very busy ... but my girls are still looking healthy

🥊 🍼Greenhouse Feeding BioGrow & Bio Enhancer ⛺️MARSHYDRO The ⛺️ has a small door 🚪 on the sides which is useful for mid section groom room work. 🤩 ☀️ MARSHYDRO FC 3000 LED 300W ☀️Also special thanks to VIPERSPECTRA P2000 (200W) & XS2000(240w) LED growlights 🌱 FASTBUDS 420

The Gorilla Glue has some rust spots on the top leaves, added some cal/mag to counteract that. Last week I thinned her out a good bit, no more fans covering bud sites more or less... Probably should have done that before she got into flower. The Gelato has recovered from the topping/fimmings and is growing its new tops, I also removed the very top leaves to keep it from growing much taller before I yoink the Gorilla Glue. Still an experiment plant, still growing better than I thought it was going to, still just sitting in the back vegetating. Day 63: This Gorilla Glue is doing markedly better than I thought it was going to. It has about 8 satellite nugs and one monster nug in the middle. The monster nug stem is zig zagged because I trained it multiple times so it's getting light on multiple levels directly to the top. I feel like there should only be 2 more weeks or so looking at the trichome production, there are some cloudy heads on the sugar leaves but still all clear on the nugs. Day 69: Don't think I'm going to feed the Gorilla Glue again, whatever happens, happens. Final feeding was 1tsp Tiger Bloom 0.5tsp cal/mag. I also removed the Gelato from the diary. It's still in there but I'll start a new diary if I decide to flower this plant.

welcome to Day 71 2/26/2021 well I am very happy she is putting on some weight and is still looking healthy. we are getting near the end of the grow now and i have a new sponsorship coming up in my next grow so stay tuned. happy growing and as always keep your stick on the ice Update with some macro shots

Ladies and gentlemen, we are moving right along. I've been having to see this girl every two days because she's in such a small pot . . She is the last plant along with the pineapple dream that I will be growing using these nutrients (Maxibloom) .. I'm not quite sure what days she's on.I guess we'll just say she's eight days older than the last time I posted lol .. anyway, she has been a breeze. As you can tell, we are going all natural, no training whatsoever..Zamnesia Seeds i'm hoping this is a good one. As is part of your exclusive tribe members list.. I hope everybody's doing well.God bless and happy growing ✌️

And off we went, the adventure with Zamnesia's F1 Hybrids started off great, with a stunning 100% germination 8/8 born without any hesitation after a very short time in water and subsequently in soil. She is precisely at the 10th, she is the Pineapple Express, she grows well regularly and very similar to the little twin. One of the characteristics of the F1 Hybrids is stability and for now it has been thoroughly demonstrated since day 10. For the rest we have to say everything is going very well, the parameters are in order, the girls were born in a biodegradable jar which biodegraded a little earlier than expected and I proceeded with the potting, no repotting, be careful the seedling never came out of the jar biodegradable the roots pass it at a certain point of decomposition. So we are now in a comfortable 11 liter house that is perfect for autoflowering in my opinion. Unfortunately, the Zamnesia pots are about to arrive but I didn't have them in time, I preferred to repot them for the sake of the plants, we need to photograph them of course but first and foremost I'm a grower and I care about the health of my little girls. Something tells me we will be growing a lot of Zammi plants so we will use them in the Official Grower version next time. Zamnesia is the best online shop in the situation, you can find everything from seeds, to mushroom kits, through vaporizers and a thousand other things https://www.zamnesia.io Pineapple Express F1 seeds specifically https://www.zamnesia.com/10681-zamnesia-seeds-pineapple-express-f1-automatic.html Site Description ZAMNESIA SEEDS - PINEAPPLE EXPRESS F1 HYBRID AUTOMATIC: FRUITY FLAVORS AND ENERGETIC EFFECTS Combining Trainwreck with Hawaiian, Pineapple Express F1 Hybrid Automatic brings a beloved strain to new heights. This autoflowering F1 hybrid cultivar ensures exceptional uniformity, reliability, and stability, making it well-suited to growers of all experience levels. If you've not checked out Pineapple Express F1 hybrid Automatic for yourself just yet, now is the time. GROWING PINEAPPLE EXPRESS F1 HYBRID AUTOMATIC All it takes is 8–9 weeks from germination before Pineapple Express F1 hybrid Automatic is ready to harvest. Thankfully, very little effort is required throughout the growing process, aside from a bit of maintenance, to ensure ample, high-quality harvests. Thanks to its F1 genetics, Pineapple Express F1 Automatic offers superb resilience and uniformity, meaning all plants will have a similar height and appearance, and will churn out comparably large yields. Reaching heights of 50–70cm, Pineapple Express F1 Automatic is not an overbearing plant and can effortlessly slot into any setup. Once ready to yield, growers can see a return in the region of 400–550g/m² indoors and up to 200g/plant outdoors. AROMAS, FLAVORS, AND EFFECTS OF PINEAPPLE EXPRESS F1 HYBRID AUTOMATIC Just one hit of Pineapple Express F1 hybrid Automatic treats users to a rich assortment of flavors and aromas. This strain offers strong pineapple notes alongside moments of citrus, spice, and pine, making for a mouth-watering blend that's extremely moreish. Bolstered by 21% THC and an array of cannabinoids, Pineapple Express F1 Automatic showcases an energetic and uplifting high that will have you feeling chatty in no time. So if you've been looking for a strain to enjoy with friends, Pineapple Express F1 Automatic could be just the thing. As for fertilizers, I'm doing a small comparative experiment to finish off the fertilizers I had at home, it's not 100% indicative, it's only on two specimens but given the stable genetics I decided to try to compare the results of Plagron Vs Aptus, both excellent brands which they will certainly produce beautiful plants which are already of excellent stable genetics. In the next few weeks the comparison will be more evident now the seedlings are still very small and just potted. So seeds of all brands, in addition to these great F1 Hybrids and the entire Zamnesia line, fertilizers and everything related to the world of cannabis, you should buy them from the best online store in the sector www.zamnesia.io

First week of flowering on a 12/12 cycle with explosive growth. Defoliated lower 7-14 inches of plants and nipped future larf buds. Top dressed with 2tsp of kelp meal this week. Next week will be worm castings (top dressed) and the following week with bloom nutrients. Gave it a good soaking with calmagged water and Mammoth microbials. California Light work's Solar Extreme 250 around 10" above the canopy. All is well! 💚

7/28 1220AM MONDAY.... Bubbles are looking ok, I need to trim them back to 4 colas, I am really stressing them with small space limitations, and temps at 80 to 81 7/29 Tuesday 5:45 AM💦💦 6:15 AM water with methylene blue two pills of nitric boost amino acid, five drops of Quick Sorb and I added bud factor X today and a quarter mL per liter of rhino silica💦💦💦. 1030 AM I don’t know what I’m gonna do. It turns out the bubbles plants are terrible. The stems are all very weak and they’re growing bushy at the top. I didn’t get them separated so all of the side branches grew up really long it’s completely useless. It is absolute this plant. It will be thrown in the garbage. Can you fucking believe I have to throw another entire plant in the fucking garbage😡😡😡😡. TREVOR plant cut down to 2 colas only, very top heavy not liking my grow space, had 2 more than I wanted so it's all fucked.... will remove shitty small colas and get maybe 2 good colas per plant, not expecting much. 1219pm....HOLY SHIT I FUCKED THESE.....TOOK OFF SOME COLAS.....NOT ENOUGH SPACE AND I PUSHED THEM TOO FAR.....I CAN FIX IT 7/30 1215am found a GREAT science link::: ""https://www.frontiersin.org/research-topics/8125/cannabis-genomics-breeding-and-production/magazine"". 345am PROLINE WILL BE HERE TODAY.ALL RESEARCH INDICATES PROLINE WILL PROTECT FROM HEAT STRESS!!!! 11:30 AM they are good again they are recovering from heavy defoliation half of the plant was taken off of one of them. I pushed it a little far but today they are recovering from heat and lack of air and lack of space the leaves are reaching up and they are not folding under heat stress. They look good today. Fast recovery heavy amino yesterday.👍👍👍👍🚨🚨🚨🚨 🚨🚨🚨🚨🚨🚨🚨🚨 GOT THE AMINOS AND HERE IS THE GROK RESULT OF MY MIX.......🚨🚨🚨🚨🚨🚨🚨🚨 The effects of applying essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine to cannabis plants can be significant, as these compounds act as biostimulants, influencing growth, stress resilience, and secondary metabolite production. The available research, primarily from studies on amino acids in plants, including cannabis, provides insights into their roles, though specific data on this exact combination is limited. Below, I summarize the effects of each compound on cannabis plants, drawing from relevant studies, and infer potential synergistic effects based on their known roles in plant physiology. Note that TMG (betaine) has less direct research in cannabis, so some effects are extrapolated from other plants. ### 1. Essential Amino Acids (EAAs) EAAs (e.g., leucine, lysine, methionine, etc.) are critical building blocks for proteins and play roles in plant metabolism and stress response. In cannabis: - **Growth and Development**: EAAs enhance protein synthesis, supporting shoot and root growth. Foliar or soil application of EAAs (e.g., 150–300 mg/L) increases leaf chlorophyll, shoot biomass, and root development in various plants, likely applicable to cannabis.[](https://www.nature.com/articles/s41598-022-21273-6) - **Nutrient Uptake**: EAAs like methionine and lysine improve nutrient absorption (e.g., nitrogen, iron) by acting as chelators, potentially enhancing cannabis nutrient efficiency in hydroponic or soil systems.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) - **Stress Mitigation**: EAAs, particularly methionine, support antioxidant production (e.g., glutathione), reducing oxidative stress from drought, salinity, or high light, common in cannabis cultivation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2020.00928/full)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cannabinoid/Terpene Production**: Limited evidence suggests EAAs may influence secondary metabolism. For example, methionine could enhance sulfur-containing compounds, potentially affecting terpene profiles in cannabis.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) **Application Notes**: EAAs are often applied via foliar spray (150–300 mg/L) or soil drench. Overuse may lead to nitrogen imbalance, so moderation is key.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### 2. Proline Proline is a non-essential amino acid that accumulates in plants under stress and serves multiple functions: - **Stress Tolerance**: Proline acts as an osmolyte, stabilizing cell membranes and scavenging reactive oxygen species (ROS) during drought, salinity, or temperature stress. In cannabis, exogenous proline (e.g., 50–100 mM) may enhance tolerance to environmental stressors, improving resilience in outdoor or controlled environments.[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW)[](https://pmc.ncbi.nlm.nih.gov/articles/PMC3548871/) - **Limited Growth Effect**: Proline alone is a poor nitrogen source for growth. Studies on Arabidopsis show it has a neutral or inhibitory effect on growth when used as the sole nitrogen source, suggesting it’s better for stress response than biomass accumulation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Cannabis-Specific**: Proline helps cannabis maintain water balance, potentially improving resistance to extreme temperatures, which is critical for outdoor grows.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) **Application Notes**: Foliar or root application (50–100 mg/L) is effective for stress mitigation. High doses may inhibit growth if not balanced with other nutrients.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full)[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW) ### 3. Trimethylglycine (TMG, Betaine) TMG is less studied in cannabis but known as an osmoprotectant and methyl donor in other plants: - **Stress Protection**: TMG stabilizes proteins and membranes under salinity, drought, or temperature stress, similar to proline. It may protect cannabis plants in harsh conditions, maintaining photosynthetic efficiency.[](https://www.mdpi.com/2311-7524/9/7/807) - **Methylation Support**: TMG donates methyl groups, potentially enhancing secondary metabolite synthesis (e.g., cannabinoids, terpenes), though direct evidence in cannabis is lacking. Its role in methylation could support metabolic pathways linked to EAAs and glutamine. - **Growth Effects**: Limited data suggest TMG may improve chlorophyll content and photosynthesis, indirectly supporting biomass in cannabis. **Application Notes**: TMG is typically applied at 0.5–2 g/L via foliar spray. Its effects in cannabis are inferred from other crops, so careful experimentation is needed. ### 4. Glutamine Glutamine is a conditionally essential amino acid central to nitrogen metabolism: - **Growth Promotion**: Glutamine (10–1000 mg/L) significantly enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and hibiscus, likely benefiting cannabis vegetative growth. In cannabis, it may support lush foliage and robust rooting in hydroponics.[](https://www.scielo.br/j/oh/a/GZxMgqXb9wm7bsCTv4WwPdc/?lang=en)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html?lang=en) - **Nitrogen Assimilation**: As a primary nitrogen acceptor, glutamine improves nitrogen use efficiency via the glutamine synthetase/glutamate synthase cycle, enhancing nutrient uptake in cannabis.[](https://academic.oup.com/jxb/article/58/9/2339/544408)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Stress Response**: Glutamine supports proline synthesis, boosting stress tolerance. It also contributes to chlorophyll and protein synthesis, improving photosynthesis and yield potential in cannabis.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Secondary Metabolism**: Glutamine is a precursor to glutamate, which influences GABA, arginine, and proline synthesis, potentially affecting cannabinoid or terpene production indirectly.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) **Application Notes**: Foliar sprays (250–1000 mg/L) or root application twice weekly are effective. Glutamine at 250 mg/L combined with other amino acids (e.g., glycine) shows synergistic growth effects.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) ### Synergistic Effects on Cannabis - **Growth and Yield**: The combination of EAAs, glutamine, and proline may enhance vegetative growth and biomass. Glutamine and EAAs drive protein synthesis and nitrogen uptake, while proline supports tissue integrity under stress. TMG likely amplifies these effects by improving cellular hydration and methylation, potentially increasing leaf area and bud quality.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.nature.com/articles/s41598-022-21273-6) - **Stress Resilience**: Proline and TMG act as osmoprotectants, while EAAs and glutamine bolster antioxidant systems, collectively improving cannabis resilience to drought, salinity, or temperature extremes common in cultivation.[](https://www.mdpi.com/2311-7524/9/7/807) - **Secondary Metabolites**: Glutamine and TMG may enhance cannabinoid and terpene production by supporting metabolic pathways (e.g., methylation, nitrogen metabolism). EAAs like methionine could influence sulfur-based terpenes. However, direct evidence for cannabinoid enhancement is sparse.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Nutrient Efficiency**: Glutamine and EAAs improve nitrogen and micronutrient uptake, while TMG may enhance nutrient transport, optimizing cannabis growth in nutrient-limited systems like hydroponics.[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) ### Potential Risks and Considerations - **Inhibition at High Doses**: High concentrations of proline or certain EAAs (e.g., lysine, valine) can inhibit growth if used as sole nitrogen sources. Balanced mixtures mitigate this, as glutamine can antagonize inhibitory effects of proline or other amino acids.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Nitrogen Overload**: Excessive EAAs or glutamine may lead to nitrogen toxicity, causing leaf burn or stunted growth in cannabis. Monitor plant response and maintain balanced nutrient solutions.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **TMG Metabolism**: TMG may increase trimethylamine production in the soil microbiome, potentially affecting cannabis root health if not managed. Healthy soil microflora can mitigate this.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **Application Timing**: Foliar sprays (150–500 mg/L for EAAs, glutamine, proline; 0.5–2 g/L for TMG) during vegetative growth or early flowering maximize benefits. Avoid overuse during late flowering to prevent nutrient imbalances affecting bud quality. ### Cannabis-Specific Insights - **Hydroponics and Soil**: In hydroponic systems, EAAs and glutamine ensure efficient nitrogen delivery, while proline and TMG support stress resistance in fluctuating pH or nutrient conditions. In soil, maintaining microflora is crucial, as it naturally supplies amino acids; supplementation should be moderate.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cultivar Variability**: Cannabis cultivars differ in nutrient demands and stress responses. Sativa-dominant strains may benefit more from proline and TMG for stress tolerance, while indica strains may respond better to EAAs and glutamine for dense bud growth. Testing is needed. - **Secondary Metabolites**: While amino acids influence secondary metabolism, direct effects on THC or CBD levels are unconfirmed. Glutamine and TMG may indirectly enhance terpene diversity via improved photosynthesis and methylation.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) ### Practical Recommendations "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨 - **Dosing**: Apply EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) via foliar spray or root drench twice weekly during vegetative growth and early flowering. - **Combination**: Use a balanced mix (e.g., 250 mg/L EAAs + 250 mg/L glutamine + 50 mg/L proline + 500 mg/L TMG) to avoid inhibition. Test on a small batch first.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Monitoring**: Check for signs of nitrogen toxicity (dark green leaves, clawing) or stress relief (improved vigor, greener leaves). Adjust doses based on plant response. "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to enhance uptake.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient lockout. In soil, ensure healthy microbial activity to complement amino acid supplementation.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### Evidence Gaps - **Specific Combination**: No studies directly address EAAs, proline, TMG, and glutamine together in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid Impact**: Limited data on how these compounds affect THC/CBD levels. More research is needed on secondary metabolite pathways.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **TMG in Cannabis**: TMG’s effects are extrapolated from other crops. Cannabis-specific trials are needed to confirm benefits. ### Conclusion Applying EAAs, proline, TMG, and glutamine to cannabis plants can enhance growth, stress tolerance, and nutrient uptake, with potential benefits for yield and secondary metabolite production. EAAs and glutamine drive biomass and nitrogen efficiency, proline and TMG bolster stress resilience, and their combination may optimize cannabis health in challenging environments. However, careful dosing and monitoring are crucial to avoid inhibition or toxicity. For best results, test small-scale applications and consult with a horticulturist familiar with cannabis cultivation. If you want more details on application methods, specific cultivars, or further searches for cannabis-related studies, let me know!""🚨🚨🚨🚨 🚨🚨 7/31 125am!!!!! Adding L-glycine to the combination of essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine can further influence the effects on cannabis plants, as glycine is a non-essential amino acid with unique roles in plant physiology. Below, I outline the potential effects of L-glycine alone and in combination with the previously mentioned compounds, focusing on their impact on cannabis growth, stress response, and secondary metabolite production. Since specific studies on this exact combination in cannabis are lacking, I’ll integrate findings from plant science and infer synergistic effects based on known mechanisms. I’ll keep it concise and tailored to cannabis cultivation. ### Effects of L-Glycine on Cannabis Plants L-Glycine is a simple amino acid involved in protein synthesis, stress response, and metabolic pathways: - **Growth Promotion**: Glycine (50–500 mg/L) enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and rice, likely benefiting cannabis vegetative growth. It serves as a nitrogen source and supports protein synthesis, complementing EAAs and glutamine. - **Stress Tolerance**: Glycine acts as a precursor to glutathione, a key antioxidant, reducing oxidative stress from drought, salinity, or high light. In cannabis, it may improve resilience in challenging environments (e.g., outdoor grows or high-intensity indoor setups). - **Secondary Metabolism**: Glycine is a precursor to purines and chlorophyll, potentially enhancing photosynthesis and energy metabolism. It may indirectly influence cannabinoid and terpene production by supporting metabolic pathways, though direct evidence in cannabis is limited. - **Nutrient Uptake**: Glycine chelates micronutrients (e.g., iron, zinc), improving their availability, which is critical for cannabis in hydroponic or nutrient-poor soil systems. **Application Notes**: Foliar sprays or root drenches (100–500 mg/L) are effective, often applied twice weekly during vegetative growth or early flowering. ### Synergistic Effects with EAAs, Proline, TMG, and Glutamine Adding L-glycine to the mix may enhance the effects on cannabis plants in the following ways: 1. **Enhanced Growth and Biomass**: - **Glycine + EAAs/Glutamine**: Glycine complements EAAs and glutamine in protein synthesis and nitrogen metabolism. Studies show glycine (250 mg/L) combined with glutamine enhances shoot and root growth in plants, likely amplifying cannabis vegetative vigor and bud development. - **Synergy**: Glycine’s role in chlorophyll synthesis boosts photosynthesis, supporting the energy demands of EAA-driven protein synthesis and glutamine’s nitrogen assimilation, potentially increasing leaf area and yield. 2. **Improved Stress Resilience**: - **Glycine + Proline/TMG**: Glycine and proline both contribute to glutathione production, enhancing antioxidant defenses. TMG’s osmoprotectant properties further stabilize cells under stress. Together, they may improve cannabis tolerance to drought, heat, or salinity, maintaining growth in adverse conditions. - **Cannabis-Specific**: This combination could be particularly beneficial for outdoor cannabis or indoor setups with inconsistent temperature/humidity, reducing leaf wilting or nutrient stress. 3. **Secondary Metabolite Production**: - **Glycine + Glutamine/TMG**: Glycine and glutamine are precursors to metabolic pathways (e.g., GABA, purines) that may influence cannabinoid and terpene synthesis. TMG’s methyl donation could enhance these pathways by supporting enzyme activity. While direct evidence is sparse, this stack may increase terpene diversity or cannabinoid yield in cannabis buds. - **EAAs Contribution**: Methionine (an EAA) and glycine may support sulfur-containing compounds, potentially enriching terpene profiles. 4. **Nutrient Efficiency**: - **Glycine + EAAs/Glutamine**: Glycine’s chelating properties enhance micronutrient uptake, complementing EAAs and glutamine’s role in nitrogen assimilation. This could optimize nutrient use in hydroponic cannabis systems, reducing nutrient lockout. - **TMG Synergy**: TMG’s role in cellular hydration may improve nutrient transport, amplifying glycine’s effects on micronutrient availability. ### Potential Risks and Considerations - **Inhibition at High Doses**: High glycine doses (1000 mg/L) may inhibit growth by disrupting nitrogen balance, especially if combined with EAAs or glutamine. Studies on Arabidopsis show glycine can be toxic as a sole nitrogen source, so balanced mixtures are crucial. - **Nitrogen Overload**: Adding glycine to EAAs and glutamine increases nitrogen input, risking toxicity (e.g., leaf burn, stunted growth) in cannabis. Monitor for dark green leaves or clawing and adjust doses. - **Competition for Absorption**: Glycine, EAAs, and glutamine may compete for root or foliar uptake, reducing efficacy. Split applications (e.g., glycine in the morning, EAAs/glutamine post-light cycle) can help. - **TMG Interaction**: Glycine and TMG both influence methylation pathways, but excessive methyl donors could disrupt metabolic balance in sensitive plants. Stick to moderate doses (e.g., 100–500 mg/L glycine, 0.5–1 g/L TMG). - **Cultivar Variability**: Cannabis strains (e.g., sativa vs. indica) differ in nutrient and stress responses. Glycine’s benefits may be more pronounced in stress-prone sativas or nutrient-sensitive hybrids. ### Practical Recommendations - **Dosing**: Combine glycine (100–500 mg/L), EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) in a balanced foliar spray or root drench. Apply twice weekly during vegetative growth and early flowering. - **Application**: Use foliar sprays for rapid uptake during vegetative stages or stress events. Root drenches suit hydroponic systems. Test on a small batch to assess plant response. - **Monitoring**: Watch for nitrogen toxicity (dark, curled leaves) or stress relief (greener, more vigorous plants). Adjust concentrations based on cultivar and growth stage. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to maximize glycine’s chelating benefits. Ensure adequate magnesium for chlorophyll synthesis. - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient imbalances. In soil, maintain healthy microbial activity, as microbes naturally produce glycine and other amino acids. ### Evidence Gaps - **Specific Combination**: No studies address glycine combined with EAAs, proline, TMG, and glutamine in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid/Terpene Impact**: While glycine may support secondary metabolism, direct effects on THC/CBD or terpene levels are unconfirmed. Cannabis-specific trials are needed. - **TMG and Glycine**: Their combined effects on methylation in cannabis are speculative, as most TMG research focuses on other crops. ### Conclusion Adding L-glycine to EAAs, proline, TMG, and glutamine likely enhances cannabis growth, stress tolerance, and nutrient efficiency by supporting protein synthesis, antioxidant production, and micronutrient uptake. The combination may improve vegetative vigor, bud quality, and resilience to environmental stressors, with potential (but unconfirmed) benefits for cannabinoid and terpene production. Careful dosing and monitoring are essential to avoid nitrogen toxicity or absorption competition. Start with low doses, test on a small scale, and consult a cannabis cultivation expert for tailored advice. If you want me to search for more studies, focus on a specific aspect (e.g., glycine’s role in terpene synthesis), or provide application protocols for a particular cannabis strain, let me know!"""

Wow what a beautiful monster she has become. I think (hope) she is done stretching. I have had to support most of the colas this week. A few had lain over towards other plants and if they were not blocking them then I left them lain over. The buds are really looking great and they are getting fatter on the daily. Thanks again for following me on my adventure with making my own seeds.