2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

On day 1 I changed out my reservoir. I lowered my nutes to 650 ppm(25% reduction). I adjusted the PH down to 6.1. My left plant has some burnt tips from either PH shift or over fertilization. My hope is that the lower ppm will help to resolve the issue before it spreads. On day 2 I adjusted the reservoirs PH from 6.3 to 5.9. On day 3 the reservoirs PH is 6.0. I installed my new 200 watt HLG Rspec. I adjusted PPFD readings to 700-1300 PPFD on all tops. On day 4 I adjusted the reservoirs PH from 5.8 to 6.2. On day 5 I adjusted the reservoirs PH from 6.4 to 6.0. I diluted my nutrients in my reservoir by removing one gallon of nutrients from the rez and and adding 2 gallons of straight tap water to the rez. This brought the nute concentration from 770 ppm to 540 ppm as the plants will be entering the ripening stage in a few days and they have too much nitrogen judging by the extremely dark green leaves and burnt leaf tips on some branches of the left side plant. The buds seem slightly smaller then my last run which may be due to an excess of nitrogen and maybe not enough light as some of my tops are taller then the others making proper PPFD challenging. All in all the girls are coming along nice and I look forward to the final weeks of flowering 😀 On day 6 I adjusted the reservoirs PH from 5.9 to 6.2. I think I have halted the nute burn as I haven't seen it progressing any more on the leaves. I will continue to monitor closely during these final weeks. The trichome production is increasing with some trichs on top of colas starting to go cloudy. The pistils are turning brown with roughly 40-50% are turning brown all signs I'm getting closer to the harvest window 😆. I dimmed my light to give 600-1000 PPFD to tops. On day 7 I reduced my temps to 75 during the day and 68 at night. I adjusted the PH of the reservoir from 6.6 to 6.1. My reservoirs ppm was too high so I diluted the rez with 2 gal of tap water. This brought it down to 480ppm. The average ppfd to all 14 tops is 604 with no top receiving more than 1000 ppfd

Ok here we are, bloom begins. All running smooth. Veggie was crazy hope to keep it up. Pheno #1 (right) seems to be more sativa than #2 love this 💐

She started taking off!! Super fast growth. In this stage i did pull down some of the branches and tied them down

one of the plants was eaten by snails 😕☹️ i have a spare of the same strain that hatches in pellets

Esta semana, poca cosa a añadir, tan solo que continuamos como la semana pasada. Riegos alternos entre abono y agua, según tabla de fertilización de JUJU Royal by BioBizz. Hemos aumentado la potencia de extracción para evitar olores.

Flushingtime😝😁

💩Alrighty Then Growmies We Are Back At it 💩 Well folks we just finished up the last run and so we are back to do it all over again 😁 So what do you say we have some fun 👈 We got some Gorilla Punch 👊 👊 👊 DAY 21 👉 Its been a really good week , lots of growth , shes just now decided to show her preflowers 👌 I believe the stretch is on 👈 So this week I've started her nutrients regiments 👌Just need to remind myself to take it easy 🤔 FC4800 from MarsHydro Lights being readjusted and chart updated .........👍👉Added an RU45 too the mix 👍 www.marshydro.ca 👉I used NutriNPK for nutrients for my grows and welcome anyone to give them a try .👈 👉 www.nutrinpk.com 👈 NutriNPK Cal MAG 14-0-14 NutriNPK Grow 28-14-14 NutriNPK Bloom 8-20-30 NutriNPK Bloom Booster 0-52-34 I GOT MULTIPLE DIARIES ON THE GO 😱 please check them out 😎 👉THANKS FOR TAKING THE TIME TO GO OVER MY DIARIES 👈

Was a bit of rain, but she handled it quite nice decided to start LST this week.

Seventh week of flowering started. I've changed the water into the pots and the nutrients ratio (more bloom and micro, less grow). The fan is turned three hours on, one off. Inkbird parameters are unchanged (20° -3° H +2°C). Plants are healthy and started to be bushy and taller. Light power @ 100%. Extractor @ 100%. D42: changed the water into the pots. Added 15 ml grow, 30ml micro and 40ml bloom. pH@6 EC 1.5 D44: added 1.5 liters of water to each pot. The plants are growing a lot, much more than I expected. Hydroponics is getting me astonished D46: added 2 liters of water to each pot.

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Buds putting on weight

Running into nutrient deficiency with magnesium and just the nutrients as a whole. I guess I didn't make my soil hot enough. Added extra topdress with BuildasoilBloom.

Was an awesome plant to grow and one of my favourite to this day , the smell of it is like you’re a kid again eating purple fun dip it’s unreal . He grew very odd at first but ended up taking off when it was around flowering 💯😁 the look of this girl is so eye catching and I cannot wait to try it out .

Week 5: A little yellowing on the leaves this week. We believe we have a Mag deficiency. Will adjust when watering. Did a flush due to this as well with Cal/Mag in the water. Defoliation: We took a lot more off this week due to the growth spurt. LST: using paper clips for LST and it is working amazingly. They have really adjusted well to it. Height (inches): GSC1 21.5; GSC2 15.5; GSC3 15; Delight 17 7.19.22: The back left GSC grew 3 inches overnight - crazy!!! Raised the light to accommodate it and placed the other girls on a box to be at her level.

7/13 It's maine-ing out. Overcast and misting. I guess it rained last night but not much. Today is my watering day although I think they could've made it until tomorrow. These drastic temp changes makes it hard to get a STEADY routine in place. I watered everything in the 20's a gallon the 10's 1/2 gallon. The 10th planet in the front was still heavy so I didn't water it. I'll keep an eye on it. Temps are much milder. I noticed a few more septoria leaves on the 10th planet which I promptly defoliated. This pheno is flowering. I could've put this week as flowering as several plants have started. I also saw ONE leaf on my chem dog that had septoria on it. ONE f-ing leaf! I was planning on treating this early anyway so I mixed one gallon of plant doctor 3 tsp gallon/gallon which was the recommendation. I'll monitor this plant and continue treating it the next two weeks. I may do foliar too but the mixture is different. It's a systemic so I don't think that matters as much. Considering getting mulch to protect from spores on the soil. My plants look GORGEOUS. I Hate to use such a nice plant as a guennie pig but I'm not going to sit here and watch it progress. I'm lucky it's really not that bad and that it's on a reveg. There's so many leaves that I would be forced to prune anyway. I'm seeing two MAYBE three leaves a morning but I want to get this under control. Last year u let a plant with septoria go and it finished but went to extracts. I've got a pretty good feeling about this plant doctor. I've used it before with good results. Only time will tell though.🤞 EDIT: WENT OVER AT ABOUT 1PM. ITS 2 NOW AND BLUE SKIES AND SUNNY. IM SUPER GLAD I WATERED AND EVEN MORE SO THAT I APPLIED THE PLANT DOCTOR HOW I DID. THE GIRLS ALL LOOKED AMAZING. THE TENTH PLANET I TREATED WITH PLANT DOCTOR LOOKS THE BEST IVE EVER SEEN IT. I FOUND ON LEAVE DEEP ON THE INTERIOR WITJ A SMALL SPOT BUT ITS SOMETHING IM SURE I MISSED THIS MORNING. IF THIS WORKS THIS GOOD ILL BE APPLYING IT TO EVERYTHING EVEN IF ITS JUST PREVENTATIVE.

D36-rain has been coming down every single day it seems. Plant is flowering along, going to spray neem oil by end of the week to deter pests. D37-neem oil sprayed on plants medium and fabric pot. D38-rain and humidity the past couple days. Weeks outlook though is a high of 97, sunny and night temp of no lower than 60. Summer season is officially here :) I have really been doing nothing to this plant except making sure it was watered and in the sunlight. Adding nutes per schedule helps tremendously also with one extra step taken out of the equation. D39-plant is growing and showing good bud sites. Nothing but abundant sunshine this week. Noticed a good amount of trichomes already forming on some leaves, as well as at the base of the side branches. D40-hand watered half gallon as it is brutally hot and sunny. Plant looks good. D41-noticed fungus gnat infestation. Applied neem like crazy to saturate medium. Heck..