Sour Diesel Haze is coming along nicely, lots of upward growth and node development. I gave her a FIM pinch this week. Expect the flowering stretch to start at any time. 👍

week intel: everything is perfect! stresses : just a little E.C stress around 1.7 once a week feeding: i feed them 3 times this week with this order : day 1 : i feed them heavy with silicate +base nutrients(calcium & micros + Bloom) about 818 ppm - 1.7 e.c to cause a little stress. day 3 : i feed them low dose of Feeding Booster + Karbo Boost around 382 ppm - 0.7 e.c to let them recover a little but not fully recover still a little stress will caused. day 5 : i feed them with low dose of Top-Max + B-52 around 250 ppm - 0.5 e.c to let them recover the stresses to get ready for another stress next week. guide of the week : know your plant tolerance limit of E.C stress once you saw the very very little yellowing on tips of leaves , that's the sign to don't go further , never reach that limit if you want a high quality buds, in my case tolerance is 1.8 and i'll reach to that level only once a week to cause precision stress , love your plants and have a happy growing!

Photo taken on day 68 / Flowering 35 ------------------------------------------------------------ 💧Watering On average I water each pot with: Day 64 : 1 Liter Day 67 : 1.5 Liter with Bio Enhancer I add Bioenhancer once every 14 days +/-. with 1 gram per liter I add very few nutrients to my water because I have already placed Bio Nutrients directly in my soil 😉 (BioGrow & BioBloom added on week 1 & 5) ------------------------------------------------------------ 🔥❄️Temperature of the week : Day : 21-24°C (Humidity : 55-70%) Night : 16-19°C ------------------------------------------------------------ 🚀Equipment of the week ⭐️ : Lamp Led 300W : power 95% at 30cm Extractor+ carbon filter ON 24/24h 2 fans ON 12/24h ------------------------------------------------------------ 📜Links : Banana Krumble seeds 🍌🌲 : https://shop.greenhouseseeds.nl/feminised-cannabis-seeds/usa-genetics/banana-krumble/ Food for your plants 🔥🔥👍 https://www.greenhousefeeding.com/ 👨‍🚀My Instagram 🌱❤️️: https://www.instagram.com/hou_stone420/ ------------------------------------------------------------ ☮️Thanks for your visit💚☮️

2 days b4 harvest

Not much going on, the buds are getting fatter. There is now a faint smell. Some buds now getring some colour. Still taking feed probably at least 2 weeks left.

----------------------------------------- ~BARNEYS FARM DOS SI DOS 33™️~ ----------------------------------------- This strain from 'Barneys Farm’s Cali Collection gets another jolt of super power with this explosive collection of terpenes fused together from some of the world’s most astounding US cannabis phenotypes. Dos Si Dos was developed from OG Kush Breath, a knockout Girl Scout Cookies pheno crossed with Face Off OG, then crossed again with their legendary Cookies Kush (OG Kush pheno x GSC) AND THAT’S ONLY HALF OF THE STORY. Add in a subtle blend of Gelato #33 to the pot with its star struck Sunset Sherbet x Thin Mint GSC heritage and boom! A new champion was born: a sativa burst of euphoric energy is followed by tranquility with sedative properties capable of happily tranquilizing an elephant, if you’re overzealous or uninitiated! DOS SI DOS 33™️ Cannabis Seeds Strain Specifications ~Type: Feminised ~Photoperiod: Normal ~Genetics: Dos Si Dos x Gelato #33 ~Effect: Relaxed, Sedative, Happy ~Outdoor Yield (g): up to 2000 ~Indoor Yield (g):up to 700 gr/m² ~Flowering Time (days): 60 - 65 ~Feminised Outdoor Harvest Month: October ~Feminised Outdoor Harvest Month Week: 1st-2nd week ~Height Indoor (cm): 90cm ~Height: Short ~Indica %: 60% ~Sativa %: 40% ~Indica/Sativa: Mostly Indica ~Taste: Mint, Lime, Spice ~Aroma: Cookies, Sherbet, Lime *Above information credit to Barneys Farm __________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ THE SETUP: ~Planted into Jiffy Peat Pellets that were hydrated with de-chlorinated water with SuperThrive added then ph'd to 6.0 @ 80℉ ~Grown 100% organic in 10g fabric pots with Mother Earth 70/30 Coco/Perlite medium amended with 2tbs/g of Down To Earth 4-4-4 / 2 cups/g of Earthworm Castings / 1tbs/g of Dr. Earth Flower Girl 3-9-4, 1tbs/g of Dr. Earth Bat Guano, 3/4 cup of Down To Earth Azomite and 1 tsp/g Down To Earth Fish Bone Meal. ~24hr light cycle during Germination / 19/5 light cycle for Vegetation and 12/12 for Flower ~Straight water ph'd @ 6.2-6.8 when needed and weekly Compost Tea's. __________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ Weekly Updates: 9/20- 💥BOOM!💥 Week Six of flower is here and my girl's in high gear, stacking her flowers and pumping out trichomes! Today I watered her with 1.5g de-chlorinated water with 5ml/g of Botanicare CalMag+ added, then ph'd to 6.2 @ 72℉. I turned her pot and plucked a couple of yellow shade leaves...the basic daily maintenance. 9/22- I didn't water her yesterday as she looked great and was 'praying' hard. Today she was given 1.5g of de-chlorinated water which was ph'd to 6.2 @ 72℉ which I gave her through her drip pan (bottom chuggin) and I also gave her pot a turn. She continues to pile on the trichomes and is developing exquisite reddish purple hairs in her flowers that look absolutely wicked! She is definitely one of the most photogenic cultivar's I've grown and is looking lovelier by the day! 😍 9/24- We're getting close to wrapping up Week Six of flower in a couple of days, which will put her around two or three more weeks from finishing, with the most exciting weeks yet to come! I didn't water yesterday and today I went ahead and Top Dressed her with 2 tbsp/g Dr. Earth Flower Girl 3-9-4, 1 tbsp/g Dr. Earth Gold Premium 4-4-4, 1/2 cup Down To Earth Bio-Fish, 1/3 cup Down To Earth High Phosphorus Bat Guano and 2 cups of Worm Castings. I watered in the Top Dress with 1.5g of de-chlorinated water which was ph'd to 6.2 @ 72℉ and let her enjoy her meal! 😜 9/26- After her heavy watering on the 24th I didn't water yesterday and when I checked her today at 'lights on' and she still had some weight to her pot and her leaves were praying hard so I held off on watering today and will hit her tomorrow with her usual 1.5g watering. ~Thanks for stopping in! Things should be getting a lot more interesting in the coming weeks...Stay lifted and be Blessed! 😎🙏~

This Starwberry Gorilla Auto is getting huge on me just under 30 inches tall at this point and flowers are starting to come in beautifully. Little worried about high humidity right now but I just may need to get a better dehumidifier for the tent 💚 This is the way

fifth week of flowering Cc

Everything is going smoothly, the buds are getting bigger day by day All the girls are bug free, they are still looking green The G13 has the strongest smell followed by the Runtz and the GDP doesn’t smell like anything for now

Chery Cola is one of my favorites and I just could not fix this one. I canceled this grow and threw most of it away. I vegged wrong and something happened to the dirt. grow a total loss... all the plants from this grow were junk, all from bugs. I had transplant issues, I got Bud Trainer cups for next time. I should have topped her but I didn't she was really really small. I have never grown this strain with any success at all.....I am starting over with a new one.... I fail at every attempt at Banana Purple Punch.... I will get it right next time!! even screwed up Apricot and that is almost impossible.... I think I will re-do this entire grow. The last one turned out beautiful.

She is getting soo wide, like i topped her and use lst, but nope. Its the nature grow of the super lemon haze, i love it. We will see how big she is getting.

@twenty20mendocino Ateam R&D Update ~ Let’s go day 91 an these girls are doing amazing! Looking nice an healthy we will let these girls recover for a few more days from the heavy defoliation and on Sunday we will be giving them the big flip , Let’s go flower!! Updates coming soon ! Sangria has been doing her thing , been drying for12 days now so Sunday we will be giving her the trim and jar her up, but updates coming soon yall!! Let’s have an amazing productive day as well as a great safe weekend, peace love an positive vibes to y’all Cheers an blaze on 😶‍🌫️💨💨💨💨

Moved the light 20cm up and it seems like she appreciated the change. Now that the temperature and the humidity level are more stable, she should grow faster. UPDATE: TOPPED! My 2 Royal Highness are still seedlings, while all the other plants (Gorilla included) are getting bigger and bigger every other day. Let’s say I had to top these ladies (also) to wait the Highnesses. Come on babies!! ❤️

Giorno 67 La pianta sta bene come puoi vedere dal colore delle foglie. Le temperature stanno scendendo e ha piovuto molto. Speriamo di non doverla raccogliere 🤞 A settimana prossima 🖐️

Week 1 Day 1 - Clones #1 and #2 are looking alright, not super strong and slight nutrient deficiency issues, but OK. Will just observe for now. - These clones are currently under a very mild CFL light (20 cm away and about 200 ppfd measured roughly by phone app - Tent Buddy.) - Clone #3 still need to be covered to maintain moisture. Yesterday I un-cupped the clone and it wilted a lot. This morning it looks better again after a night of rest under the plastic cup. - I've checked the run off and these clone's pH are so low, while TDS are so high (pH 5.7 and TDS 800pm). This evening, I flushed them with pH 6.5 water and brought the runoff to about pH 6.0 and 350 ppm) Week 1 Day 2 - Just foliage spray with mild CalMag this morning. Soil is still moist, so no watering. Week 1 Day 3 - Just minor watering today. Glad to see the clones are doing well as they are growing a bit everyday. Will transfer them to 3 gallon airpots with new soil mix soon. Week 1 Day 4 - Foliage spray with mild CalMag and Seaweed. Add 5ml of 250 ppm of balanced NPK nutrients AN B52 for each plant. Week 1 Day 5 - Foliage spray with mild CalMag and Seaweed. Transfer them to 3 gallon airpots. Week 1 Day 6 - Foliage spray with mild CalMag and Seaweed. Skip watering. The clones responded OK to the new airpots. Week 1 Day 7 - TBA

Had some issues, she got too big for her 5 gallon pot. Her roots were poking out of the bottom of the fabric. Had to transplant and prune the roots. Now she’s in a 20 gallon and she’s being flipped to flower. As you see she bounced back just fine.

7/28 1220AM MONDAY.... Bubbles are looking ok, I need to trim them back to 4 colas, I am really stressing them with small space limitations, and temps at 80 to 81 7/29 Tuesday 5:45 AM💦💦 6:15 AM water with methylene blue two pills of nitric boost amino acid, five drops of Quick Sorb and I added bud factor X today and a quarter mL per liter of rhino silica💦💦💦. 1030 AM I don’t know what I’m gonna do. It turns out the bubbles plants are terrible. The stems are all very weak and they’re growing bushy at the top. I didn’t get them separated so all of the side branches grew up really long it’s completely useless. It is absolute this plant. It will be thrown in the garbage. Can you fucking believe I have to throw another entire plant in the fucking garbage😡😡😡😡. TREVOR plant cut down to 2 colas only, very top heavy not liking my grow space, had 2 more than I wanted so it's all fucked.... will remove shitty small colas and get maybe 2 good colas per plant, not expecting much. 1219pm....HOLY SHIT I FUCKED THESE.....TOOK OFF SOME COLAS.....NOT ENOUGH SPACE AND I PUSHED THEM TOO FAR.....I CAN FIX IT 7/30 1215am found a GREAT science link::: ""https://www.frontiersin.org/research-topics/8125/cannabis-genomics-breeding-and-production/magazine"". 345am PROLINE WILL BE HERE TODAY.ALL RESEARCH INDICATES PROLINE WILL PROTECT FROM HEAT STRESS!!!! 11:30 AM they are good again they are recovering from heavy defoliation half of the plant was taken off of one of them. I pushed it a little far but today they are recovering from heat and lack of air and lack of space the leaves are reaching up and they are not folding under heat stress. They look good today. Fast recovery heavy amino yesterday.👍👍👍👍🚨🚨🚨🚨 🚨🚨🚨🚨🚨🚨🚨🚨 GOT THE AMINOS AND HERE IS THE GROK RESULT OF MY MIX.......🚨🚨🚨🚨🚨🚨🚨🚨 The effects of applying essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine to cannabis plants can be significant, as these compounds act as biostimulants, influencing growth, stress resilience, and secondary metabolite production. The available research, primarily from studies on amino acids in plants, including cannabis, provides insights into their roles, though specific data on this exact combination is limited. Below, I summarize the effects of each compound on cannabis plants, drawing from relevant studies, and infer potential synergistic effects based on their known roles in plant physiology. Note that TMG (betaine) has less direct research in cannabis, so some effects are extrapolated from other plants. ### 1. Essential Amino Acids (EAAs) EAAs (e.g., leucine, lysine, methionine, etc.) are critical building blocks for proteins and play roles in plant metabolism and stress response. In cannabis: - **Growth and Development**: EAAs enhance protein synthesis, supporting shoot and root growth. Foliar or soil application of EAAs (e.g., 150–300 mg/L) increases leaf chlorophyll, shoot biomass, and root development in various plants, likely applicable to cannabis.[](https://www.nature.com/articles/s41598-022-21273-6) - **Nutrient Uptake**: EAAs like methionine and lysine improve nutrient absorption (e.g., nitrogen, iron) by acting as chelators, potentially enhancing cannabis nutrient efficiency in hydroponic or soil systems.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) - **Stress Mitigation**: EAAs, particularly methionine, support antioxidant production (e.g., glutathione), reducing oxidative stress from drought, salinity, or high light, common in cannabis cultivation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2020.00928/full)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cannabinoid/Terpene Production**: Limited evidence suggests EAAs may influence secondary metabolism. For example, methionine could enhance sulfur-containing compounds, potentially affecting terpene profiles in cannabis.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) **Application Notes**: EAAs are often applied via foliar spray (150–300 mg/L) or soil drench. Overuse may lead to nitrogen imbalance, so moderation is key.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### 2. Proline Proline is a non-essential amino acid that accumulates in plants under stress and serves multiple functions: - **Stress Tolerance**: Proline acts as an osmolyte, stabilizing cell membranes and scavenging reactive oxygen species (ROS) during drought, salinity, or temperature stress. In cannabis, exogenous proline (e.g., 50–100 mM) may enhance tolerance to environmental stressors, improving resilience in outdoor or controlled environments.[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW)[](https://pmc.ncbi.nlm.nih.gov/articles/PMC3548871/) - **Limited Growth Effect**: Proline alone is a poor nitrogen source for growth. Studies on Arabidopsis show it has a neutral or inhibitory effect on growth when used as the sole nitrogen source, suggesting it’s better for stress response than biomass accumulation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Cannabis-Specific**: Proline helps cannabis maintain water balance, potentially improving resistance to extreme temperatures, which is critical for outdoor grows.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) **Application Notes**: Foliar or root application (50–100 mg/L) is effective for stress mitigation. High doses may inhibit growth if not balanced with other nutrients.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full)[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW) ### 3. Trimethylglycine (TMG, Betaine) TMG is less studied in cannabis but known as an osmoprotectant and methyl donor in other plants: - **Stress Protection**: TMG stabilizes proteins and membranes under salinity, drought, or temperature stress, similar to proline. It may protect cannabis plants in harsh conditions, maintaining photosynthetic efficiency.[](https://www.mdpi.com/2311-7524/9/7/807) - **Methylation Support**: TMG donates methyl groups, potentially enhancing secondary metabolite synthesis (e.g., cannabinoids, terpenes), though direct evidence in cannabis is lacking. Its role in methylation could support metabolic pathways linked to EAAs and glutamine. - **Growth Effects**: Limited data suggest TMG may improve chlorophyll content and photosynthesis, indirectly supporting biomass in cannabis. **Application Notes**: TMG is typically applied at 0.5–2 g/L via foliar spray. Its effects in cannabis are inferred from other crops, so careful experimentation is needed. ### 4. Glutamine Glutamine is a conditionally essential amino acid central to nitrogen metabolism: - **Growth Promotion**: Glutamine (10–1000 mg/L) significantly enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and hibiscus, likely benefiting cannabis vegetative growth. In cannabis, it may support lush foliage and robust rooting in hydroponics.[](https://www.scielo.br/j/oh/a/GZxMgqXb9wm7bsCTv4WwPdc/?lang=en)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html?lang=en) - **Nitrogen Assimilation**: As a primary nitrogen acceptor, glutamine improves nitrogen use efficiency via the glutamine synthetase/glutamate synthase cycle, enhancing nutrient uptake in cannabis.[](https://academic.oup.com/jxb/article/58/9/2339/544408)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Stress Response**: Glutamine supports proline synthesis, boosting stress tolerance. It also contributes to chlorophyll and protein synthesis, improving photosynthesis and yield potential in cannabis.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Secondary Metabolism**: Glutamine is a precursor to glutamate, which influences GABA, arginine, and proline synthesis, potentially affecting cannabinoid or terpene production indirectly.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) **Application Notes**: Foliar sprays (250–1000 mg/L) or root application twice weekly are effective. Glutamine at 250 mg/L combined with other amino acids (e.g., glycine) shows synergistic growth effects.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) ### Synergistic Effects on Cannabis - **Growth and Yield**: The combination of EAAs, glutamine, and proline may enhance vegetative growth and biomass. Glutamine and EAAs drive protein synthesis and nitrogen uptake, while proline supports tissue integrity under stress. TMG likely amplifies these effects by improving cellular hydration and methylation, potentially increasing leaf area and bud quality.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.nature.com/articles/s41598-022-21273-6) - **Stress Resilience**: Proline and TMG act as osmoprotectants, while EAAs and glutamine bolster antioxidant systems, collectively improving cannabis resilience to drought, salinity, or temperature extremes common in cultivation.[](https://www.mdpi.com/2311-7524/9/7/807) - **Secondary Metabolites**: Glutamine and TMG may enhance cannabinoid and terpene production by supporting metabolic pathways (e.g., methylation, nitrogen metabolism). EAAs like methionine could influence sulfur-based terpenes. However, direct evidence for cannabinoid enhancement is sparse.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Nutrient Efficiency**: Glutamine and EAAs improve nitrogen and micronutrient uptake, while TMG may enhance nutrient transport, optimizing cannabis growth in nutrient-limited systems like hydroponics.[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) ### Potential Risks and Considerations - **Inhibition at High Doses**: High concentrations of proline or certain EAAs (e.g., lysine, valine) can inhibit growth if used as sole nitrogen sources. Balanced mixtures mitigate this, as glutamine can antagonize inhibitory effects of proline or other amino acids.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Nitrogen Overload**: Excessive EAAs or glutamine may lead to nitrogen toxicity, causing leaf burn or stunted growth in cannabis. Monitor plant response and maintain balanced nutrient solutions.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **TMG Metabolism**: TMG may increase trimethylamine production in the soil microbiome, potentially affecting cannabis root health if not managed. Healthy soil microflora can mitigate this.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **Application Timing**: Foliar sprays (150–500 mg/L for EAAs, glutamine, proline; 0.5–2 g/L for TMG) during vegetative growth or early flowering maximize benefits. Avoid overuse during late flowering to prevent nutrient imbalances affecting bud quality. ### Cannabis-Specific Insights - **Hydroponics and Soil**: In hydroponic systems, EAAs and glutamine ensure efficient nitrogen delivery, while proline and TMG support stress resistance in fluctuating pH or nutrient conditions. In soil, maintaining microflora is crucial, as it naturally supplies amino acids; supplementation should be moderate.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cultivar Variability**: Cannabis cultivars differ in nutrient demands and stress responses. Sativa-dominant strains may benefit more from proline and TMG for stress tolerance, while indica strains may respond better to EAAs and glutamine for dense bud growth. Testing is needed. - **Secondary Metabolites**: While amino acids influence secondary metabolism, direct effects on THC or CBD levels are unconfirmed. Glutamine and TMG may indirectly enhance terpene diversity via improved photosynthesis and methylation.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) ### Practical Recommendations "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨 - **Dosing**: Apply EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) via foliar spray or root drench twice weekly during vegetative growth and early flowering. - **Combination**: Use a balanced mix (e.g., 250 mg/L EAAs + 250 mg/L glutamine + 50 mg/L proline + 500 mg/L TMG) to avoid inhibition. Test on a small batch first.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Monitoring**: Check for signs of nitrogen toxicity (dark green leaves, clawing) or stress relief (improved vigor, greener leaves). Adjust doses based on plant response. "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to enhance uptake.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient lockout. In soil, ensure healthy microbial activity to complement amino acid supplementation.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### Evidence Gaps - **Specific Combination**: No studies directly address EAAs, proline, TMG, and glutamine together in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid Impact**: Limited data on how these compounds affect THC/CBD levels. More research is needed on secondary metabolite pathways.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **TMG in Cannabis**: TMG’s effects are extrapolated from other crops. Cannabis-specific trials are needed to confirm benefits. ### Conclusion Applying EAAs, proline, TMG, and glutamine to cannabis plants can enhance growth, stress tolerance, and nutrient uptake, with potential benefits for yield and secondary metabolite production. EAAs and glutamine drive biomass and nitrogen efficiency, proline and TMG bolster stress resilience, and their combination may optimize cannabis health in challenging environments. However, careful dosing and monitoring are crucial to avoid inhibition or toxicity. For best results, test small-scale applications and consult with a horticulturist familiar with cannabis cultivation. If you want more details on application methods, specific cultivars, or further searches for cannabis-related studies, let me know!""🚨🚨🚨🚨 🚨🚨 7/31 125am!!!!! Adding L-glycine to the combination of essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine can further influence the effects on cannabis plants, as glycine is a non-essential amino acid with unique roles in plant physiology. Below, I outline the potential effects of L-glycine alone and in combination with the previously mentioned compounds, focusing on their impact on cannabis growth, stress response, and secondary metabolite production. Since specific studies on this exact combination in cannabis are lacking, I’ll integrate findings from plant science and infer synergistic effects based on known mechanisms. I’ll keep it concise and tailored to cannabis cultivation. ### Effects of L-Glycine on Cannabis Plants L-Glycine is a simple amino acid involved in protein synthesis, stress response, and metabolic pathways: - **Growth Promotion**: Glycine (50–500 mg/L) enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and rice, likely benefiting cannabis vegetative growth. It serves as a nitrogen source and supports protein synthesis, complementing EAAs and glutamine. - **Stress Tolerance**: Glycine acts as a precursor to glutathione, a key antioxidant, reducing oxidative stress from drought, salinity, or high light. In cannabis, it may improve resilience in challenging environments (e.g., outdoor grows or high-intensity indoor setups). - **Secondary Metabolism**: Glycine is a precursor to purines and chlorophyll, potentially enhancing photosynthesis and energy metabolism. It may indirectly influence cannabinoid and terpene production by supporting metabolic pathways, though direct evidence in cannabis is limited. - **Nutrient Uptake**: Glycine chelates micronutrients (e.g., iron, zinc), improving their availability, which is critical for cannabis in hydroponic or nutrient-poor soil systems. **Application Notes**: Foliar sprays or root drenches (100–500 mg/L) are effective, often applied twice weekly during vegetative growth or early flowering. ### Synergistic Effects with EAAs, Proline, TMG, and Glutamine Adding L-glycine to the mix may enhance the effects on cannabis plants in the following ways: 1. **Enhanced Growth and Biomass**: - **Glycine + EAAs/Glutamine**: Glycine complements EAAs and glutamine in protein synthesis and nitrogen metabolism. Studies show glycine (250 mg/L) combined with glutamine enhances shoot and root growth in plants, likely amplifying cannabis vegetative vigor and bud development. - **Synergy**: Glycine’s role in chlorophyll synthesis boosts photosynthesis, supporting the energy demands of EAA-driven protein synthesis and glutamine’s nitrogen assimilation, potentially increasing leaf area and yield. 2. **Improved Stress Resilience**: - **Glycine + Proline/TMG**: Glycine and proline both contribute to glutathione production, enhancing antioxidant defenses. TMG’s osmoprotectant properties further stabilize cells under stress. Together, they may improve cannabis tolerance to drought, heat, or salinity, maintaining growth in adverse conditions. - **Cannabis-Specific**: This combination could be particularly beneficial for outdoor cannabis or indoor setups with inconsistent temperature/humidity, reducing leaf wilting or nutrient stress. 3. **Secondary Metabolite Production**: - **Glycine + Glutamine/TMG**: Glycine and glutamine are precursors to metabolic pathways (e.g., GABA, purines) that may influence cannabinoid and terpene synthesis. TMG’s methyl donation could enhance these pathways by supporting enzyme activity. While direct evidence is sparse, this stack may increase terpene diversity or cannabinoid yield in cannabis buds. - **EAAs Contribution**: Methionine (an EAA) and glycine may support sulfur-containing compounds, potentially enriching terpene profiles. 4. **Nutrient Efficiency**: - **Glycine + EAAs/Glutamine**: Glycine’s chelating properties enhance micronutrient uptake, complementing EAAs and glutamine’s role in nitrogen assimilation. This could optimize nutrient use in hydroponic cannabis systems, reducing nutrient lockout. - **TMG Synergy**: TMG’s role in cellular hydration may improve nutrient transport, amplifying glycine’s effects on micronutrient availability. ### Potential Risks and Considerations - **Inhibition at High Doses**: High glycine doses (1000 mg/L) may inhibit growth by disrupting nitrogen balance, especially if combined with EAAs or glutamine. Studies on Arabidopsis show glycine can be toxic as a sole nitrogen source, so balanced mixtures are crucial. - **Nitrogen Overload**: Adding glycine to EAAs and glutamine increases nitrogen input, risking toxicity (e.g., leaf burn, stunted growth) in cannabis. Monitor for dark green leaves or clawing and adjust doses. - **Competition for Absorption**: Glycine, EAAs, and glutamine may compete for root or foliar uptake, reducing efficacy. Split applications (e.g., glycine in the morning, EAAs/glutamine post-light cycle) can help. - **TMG Interaction**: Glycine and TMG both influence methylation pathways, but excessive methyl donors could disrupt metabolic balance in sensitive plants. Stick to moderate doses (e.g., 100–500 mg/L glycine, 0.5–1 g/L TMG). - **Cultivar Variability**: Cannabis strains (e.g., sativa vs. indica) differ in nutrient and stress responses. Glycine’s benefits may be more pronounced in stress-prone sativas or nutrient-sensitive hybrids. ### Practical Recommendations - **Dosing**: Combine glycine (100–500 mg/L), EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) in a balanced foliar spray or root drench. Apply twice weekly during vegetative growth and early flowering. - **Application**: Use foliar sprays for rapid uptake during vegetative stages or stress events. Root drenches suit hydroponic systems. Test on a small batch to assess plant response. - **Monitoring**: Watch for nitrogen toxicity (dark, curled leaves) or stress relief (greener, more vigorous plants). Adjust concentrations based on cultivar and growth stage. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to maximize glycine’s chelating benefits. Ensure adequate magnesium for chlorophyll synthesis. - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient imbalances. In soil, maintain healthy microbial activity, as microbes naturally produce glycine and other amino acids. ### Evidence Gaps - **Specific Combination**: No studies address glycine combined with EAAs, proline, TMG, and glutamine in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid/Terpene Impact**: While glycine may support secondary metabolism, direct effects on THC/CBD or terpene levels are unconfirmed. Cannabis-specific trials are needed. - **TMG and Glycine**: Their combined effects on methylation in cannabis are speculative, as most TMG research focuses on other crops. ### Conclusion Adding L-glycine to EAAs, proline, TMG, and glutamine likely enhances cannabis growth, stress tolerance, and nutrient efficiency by supporting protein synthesis, antioxidant production, and micronutrient uptake. The combination may improve vegetative vigor, bud quality, and resilience to environmental stressors, with potential (but unconfirmed) benefits for cannabinoid and terpene production. Careful dosing and monitoring are essential to avoid nitrogen toxicity or absorption competition. Start with low doses, test on a small scale, and consult a cannabis cultivation expert for tailored advice. If you want me to search for more studies, focus on a specific aspect (e.g., glycine’s role in terpene synthesis), or provide application protocols for a particular cannabis strain, let me know!"""

Harvested the more mature plant, it had lost it's top to the wind, harvested a few days ago and has had a couple spots with a little rot removed. Seems stable enough and mature. Going to let the remaining one run out a bit as it seems a bit slower.