This week i defoliated a bit, as i wanted to have more light reach lower parts of the plant to increase bud sights and increase air flow through out the plants. Also I put in the net again in a nother attempt to even out the cannopy which didn't really work

Great harvest, I was impressed with the quality of this strain. After the drying and curing process, I'll give my feedback! 💪

Eccoci qui... Tutto va per il meglio, questa settimana si inizia a vedere la resina e ora si inizieranno a formare le cime, per questo aggiungo Sugar Shot di @xpertnutrients vedremo l'evoluzione settimana prossima. Odore intenso, fortunatamente ne ho messi 2 di semi ed ora posso godermi queste bellezze che sono SPAZIALI. Mi fanno impazzire sia per l'odore che per l conformazione di entrambe. Grazie a tutti per il supporto🔥🌲❤️

The third week is over the stress of repotting is well absorbed and growth is in full swing. Light Power: 50% Day 16 Photoshooting

_______________________________________________________ Mi. 25.3. - 3,5 Liter pro Pflanze gegossen (ca. 300ml Drain) - Rainpoint Bewässerungssystem eingesetzt - 1L auf 3 Pflanzen Nährlösung per Hand angegossen - Programm: 3min. ON / 3min. PAUSE / 3min. ON - 2L pro Zyklus. - 1L klar spülen zur Systemreinigung 1,2 ml / Liter "Calmag" 1,5 ml / Liter "Bio Grow" 4,0 ml / Liter "Bio Bloom" 4,0 ml / Liter "Top Max" Ph 6,2 / 790 ppm _______________________________________________________ So. 29.3. - 3,2 Liter pro Pflanze gegossen (ca. 200ml Drain) - 1L Nährlösung per Hand angegossen - 8L Nährlösung per Gießsystem - 0,5L klar spülen zur Systemreinigung 1,2 ml / Liter "Calmag" 1,5 ml / Liter "Bio Grow" 4,0 ml / Liter "Bio Bloom" 4,0 ml / Liter "Top Max" Ph 6,2 / 770 ppm _______________________________________________________

week 4 plants look nice slight nitrogen def pumped up the dosage on the nutes

Este próximo día 14/06/23 entraremos en la 3º semana de crecimiento. A día 12/06/23 llevamos 28 días desde que se empezó a germinar. ya están todas trasplantadas a la macetas definitiva de 7 litros. Las he regado con roots + crecimiento + calmag + enzym. Todo de BOOM nutrients. He realizado el trasplante en 1 hora y 50 minutos en la mañana del lunes 12/06/23 en directo, en twitch.tv/xmackobox

This plant was fairly easy to grow, I know I can get better results with the strain but I need to go bigger. Im going to pop another Red Devil Auto seed from WSE and redo this diary right away.

Created a new setup..took time away from my diary keeping... But everything is good..buds are thick and dense... Flush begins Day 5. Flush for 1 week and harvest

All the plants are loving the conditions in the greenhouse getting alot of movemnt after feed and loads of watering done due to greenhouse neing so hot hit with biobizz bloom and grow some. Rockdust aswell

Defoliación leve🍀👨‍🌾🏻

Week 10: Buds are stacking and the frost is building! Observations: Week 10 is in the books and the girls are looking beautiful! The bud sites are really starting to fatten up and stack nicely. We are seeing some great trichome production developing on the sugar leaves, giving them a nice frosty look. Looking closely at the pistils, we have a mix right now. Many are still bright white and shooting straight out, which means the calyxes are still swelling and the plant is actively packing on weight. However, on some of the more mature colas, the pistils are just starting to darken, turn orange/brown, and curl inward. Plant Health & Nutrients: Overall, the canopy looks healthy and vigorous. The fan leaves are a very deep, rich, dark green. I did notice some very minor nutrient burn (crispy brown tips) on the edges of some leaves. It's nothing major to worry about, but it tells me the plant is right at its absolute limit for nutrients. I might dial back the feeding/EC slightly (especially the Nitrogen, given how dark the leaves are) so I don't stress her out as she finishes up flower. Next Steps: Going to keep the environment dialed in, keep an eye on those leaf tips, and just let her do her thing! The smell is really starting to come through now.

Getting bigger and looking healthy, only around 150 PPFD They seem to be sensitive to strong light, still too little Day 21 - topped the bigger girl, hopefully she catches up since she was already moving faster

Got a 2nd alaskan purp thas goin weird in mr. Other then thst all lookin good. Buds comin in everywhere. Bringing out the tent fabric and ladybugs to make sure ee finish strong and disease/mold free 😁. Been waitin on the rain before i bring out the clear plastic but not sure if ill need it this year. Gotta love this michigan weather.

This last week has been a good one. I've been watching the girls get so much fatter, it's actually awesome haha All 3 ladies look beautiful & are starting to display signs of the end in the leaves. I imagine next weeks flush will exacerbate these signs too. I dropped my feed down slightly as per the Professors Nutrients feeding schedule & pumped up the watering to 1L per day. The plants had no issues with taking the extra water & I would imagine it's helping the buds get that much thicker. I started defoliating mid week & particularily on Plant 3 to clear out any bigger leaves & leaves gaps to hopefully be filled + give the bigger buds some air. She's gotten the least amount of defoliation this grow as she's at the back of my tent. But she also has by far the bigger buds... so a good lesson learned for future grows. I also cut all LST wires at the end of this week & now all plants are holding their own. All plants trichs are looking nice & Plant 3 has more amber in the colouring, it also happens to have the bigger colas 😏 Plant's 1 & 2 trichs however appear to be more dense. The change of light cycle from last week has seemed beneficial with no negatives sighted, which has been good. It's hard to believe that this coming week is the start of the flush & my next post will be harvest. It's exciting! Check back for the harvest ✌️🏼

👌🏻👌🏻

5/23/2023 Week 7- Day 1 of Flower (Day 110 overall) (Day 49 of Flower) There was a couple of small super small sucker buds on the lower stocks that needed to be cleaned off but that was it. Just watching them Flower!! 5/24/2023 Week 7- Day 2 of Flower (Day 111 overall) Tucked a few fan leaves below lower buds but didn't see any blatant offenders that needed to be removed. I typically do start my checks in Week 8 but since this is a new strain I decided to go ahead and do a Trichome check just to see what was what.. Just as I thought nothing but clear glassy Trichomes. I will check again in one week.... 5/25/2023 Week 7- Day 3 of Flower (Day 112 overall) Just letting them grow today!! Smell is getting stronger I would say it is a hint of Pine and a heavier fruity smell. 5/26/2023 Week 7- Day 4 of Flower (Day 113 overall) I did Nothing but took some pics and I enjoyed it!!!! It is nice to be able to just sit back and watch them on auto pilot.. :-) Tomorrow Water Change Day!! 5/27/2023 Week 7- Day 5 of Flower (Day 114 overall) I admit it.. I had to defoliate a little bit.. I looked in while changing the water today and there were just a couple blatant offenders blocking the light from lower bud sites and those leaves they just had to go. Other than that I changed the water and this week is where I drop everything and start preparing them for this last phase of the grow. Loving a few of these buds they are starting to beef out very nicely. I added 36 gallons Mammoth Silica = .5Mil/Gal= 18Mil Root DRIP = 1Mil/Gal= 36Mil CalMag= .5Mil/Gal= 18Mil FloraMicro = 3.8Mil/Gal= 137Mil FloraGro= 2.8Mil/Gal= 100Mil FLoraBloom= 4.6Mil/Gal= 166Mil LiquidKoolBloom= 2.5Mil/Gal= 90Mil TPS SIGNAL= 4.0Mil/Gal= 144Mil ORCA = .5Mil/Gal= 18Mil Water Temp= 70.1 Tent Temp= 76 Humidity =64 ** I have two dehumidifiers running on Ink bird controllers to try and keep the humidity down, In late in flower I like to keep the humidity above 45 but below 60. 5/28/2023 Week 7- Day 6 of Flower (Day 115 overall) Opened the back of the tent today and boy did she need some defoliation love in the back. Other than that I took off a few little sucker buds trying to form. I did start to setup my Dry space as well as did a Trichome Check today and the brown tips are coming in nicely. 5/29/2023 Week 7- Day 7 of Flower (Day 116 overall) Found a few baby sucker buds trying to form so I removed them. Other than that I am enjoying watching her mature. I also started my Reverse of the Clone I took 6 weeks ago in Apr from #3. I am uploading footage of the reversing process for your viewing pleasure.

All content on this diary is for inspirational and educational purposes only. The ideas shared are not a substitute for professional advice. This diary/account is not officially affiliated with Alan Watts or his estate. All materials are used under the principles of fair use. I honor the legacy of Alan Watts by sharing his wisdom respectfully and with the intention of inspiring awareness and self-understanding. 2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.