First grow ever, may the auto gods bless this grow 🙏

Finally decent temperatures and we can start the flower! They recovered well from the temperature shock and show some growth. They got a massive haircut and trained. Now they can enter flower stage. Each plant got 50 g of Wizzy's Flower 6-8-10 organic fertilizer, 3 g / 10 L of Plant Booster and 1g / Liter Weedtamines. A few days ago. Tomorrow they'll get their dose of Pflanzengeflüster (Plant Whisper) to increase flower production.

| 📅 Week 3 | Days 15 - 21 | 💡 562w 📏38' | | 🌡️️🌞 78 - 82 | | 🌡️️🌑 73 - 77 | 11/23 Reservoir swap with increased nutrients and PH adjusted to 5.8. The plants have been spread out a little more to give them space. 11/24 Temperatures are fine however humidity could use a bump. PH in reservoirs adjusted to 5.6. 11/25 PH adjusted to 5.8 and reservoirs topped off with water. The PH fluctuations have been rough for this strain. It doesn't seem to like the Hydros wavering PH. 11/26 The light was raised a few inches to match growth. PH adjusted to 5.8 and the humidity is still lower than I would like it to be. 11/27 Reservoirs topped off and PH set to 5.8. Fans adjusted to better circulate the air around the tent. 11/28 Daily PHing and reservoir topping as usual. The back right plant is still doing its silly genetic thing as i'm sure it will all grow. Debating on cutting it early and saving the time and tent space. 11/29 PH adjusted to 5.8 and a single reservoir needing topped off. Plants appears to be rebounding from PH fluctuations but its to early to know for certain. This marks the end of week 3 and the growth was fine and fast like expected from this breeder. The plants have started showing sign of PH fluctuations as these genetics don't seem to enjoy hydroponics to much, however I'm confident in them developing resiliency as they grow and getting past it. I am debating doing a PH twice daily to minimize the PH window. One shows nitrogen toxicity. With these being Autoflowers anything that slows them down is a problem so we are going to see what we can do to minimize the effects the fluctuations have on these plants. I'm confident they will be dank in a few weeks 😎

Ph meter is broken so I’ve just been feeding plain water, going to try order one ASAP. I also forgot to lower the light a few days ago, checked on them two days ago and three of them had fallen over so I had to tie them up with garden ties and adjust the distance of the light. Fastberry seems to be a bit behind the others, I might have overwatered a little bit the other day, hopefully it bounces back sooner than later. Ph meter has come will be feeding nutrients for the first time with their next feed

Day 44 - March 25, 2021 Started noticing a couple gnats in our house. It's been warm outside and they are starting to come out. Unfortunately saw some in the room as well and can only assume there are many more feeding on the ladies. Gave foliar spray using Bee Safe Organicide. Gave all the girls a thorough haircut today and worked on trying to get canopy under control. Vegatation was very thick. Doesn't look like deficency issue with F7 is spreading. Continuing to watch for any changes indicating the problem is still ongoing. Gave foliar spray (1:00AM): * Bee Safe Organicide Watering: 03:00AM-3:10AM ~0.33 gallons per pot 11:00AM-11:10AM ~0.33 gallons per pot 7:00PM-7:10PM ~0.33 gallons per pot Reservoir: * tested 10:45PM * 2.21 EC * 5.9 PH * 73.94F (with ambient temps being higher, res temp is higher as well) * ~13-gallons remaining before topping off * Added ~5-gallons (solution mixed 3/24) (11:00PM) * 2.16 EC (after topping off) * 5.9 pH (after topping off) * 73.40F (after topping off) * ~18-gallons remaining (after topping off) Lights: * Gavita LEDs On 20-hours (8:00AM - 4:00AM) ** 8:00AM Lights On (100%) ** 1:00AM-4:00AM (Reduced light intensity to 50% for foliar spray) * Gavita LEDs Off 4-hours (4:00AM - 8:00AM) * UV Bars Off 24-hours Air control: 69F-82F (80F AVG) (Lights On) 66F (Lights Off) 50%-65% (56% AVG) humidity (Lights On) 60%-70% RH (Lights Off) ~1181PPM CO2 (Lights On) ~690PPM CO2 (Lights Off) Day 45 - March 26, 2021 Didn't do much in the garden today. Tucked a couple branches under trellis netting and that's pretty much it. Hoping will have the trellis on each cage filled out by next weekend. Nutrient deficiency with F7 seems to be resolved. New growth not showing any issues. Found a dead gnat on a leaf. Bee Safe foliar spray seems to have done the trick. Continuing to keep close eye out for pests. Mixed of nutrient solution (11:00PM): ** 50-gallons RO water (0.06 EC) ** 10ML h2o2 per gallon ** 5ML Botanicare Cal-mag+ per gallon (0.54 EC after Cal-Mag) ** 0.5ML Mills Nutrients Vitalize per gallon ** 10ML Mills Nutrients Basis A & B per gallon ** 4ML Mills Nutrients Start-R per gallon ** 1.25ML pHup per gallon (bring PH from ~5.1 to 5.8) ** ~2.02 EC ** 5.8/5.9 pH Watering: 03:00AM-3:10AM ~0.33 gallons per pot 11:00AM-11:10AM ~0.33 gallons per pot 7:00PM-7:10PM ~0.33 gallons per pot Reservoir: * tested 11:30PM * 2.16 EC * 5.8/5.9 PH * 73.94F (with ambient temps being higher, res temp is higher as well) * ~10-gallons remaining * Didn't top off today Lights: * Gavita LEDs On 20-hours (8:00AM - 4:00AM) ** 8:00AM Lights On (100%) * Gavita LEDs Off 4-hours (4:00AM - 8:00AM) * UV Bars On 8-hours (12:00PM - 8:00PM) Air control: 69F-82F (81F AVG) (Lights On) 66F (Lights Off) 50%-65% (60% AVG) humidity (Lights On) 60%-70% RH (Lights Off) ~1268PPM CO2 (Lights On) ~620PPM CO2 (Lights Off) Day 46 - March 27, 2021 Did some more pruning to remove thick foliage and open up nodes. Continuing to tuck branches through trellis. Expect will be ready to go into flower in apprxomately a week. CO2 tank ran out and co2 levels dropped to 330PPM for the first couple of hours of lights on. Replaced tank at 10AM. Watering: 03:00AM-3:10AM ~0.33 gallons per pot 11:00AM-11:10AM ~0.33 gallons per pot 7:00PM-7:10PM ~0.33 gallons per pot Reservoir: * topped off with ~18-gallons * tested 9:30PM * 2.06 EC * 5.9 PH * 73.94F (with ambient temps being higher, res temp is higher as well) * ~20-gallons remaining Lights: * Gavita LEDs On 20-hours (8:00AM - 4:00AM) ** 8:00AM Lights On (100%) * Gavita LEDs Off 4-hours (4:00AM - 8:00AM) * UV Bars On 8-hours (12:00PM - 8:00PM) Air control: 69F-82F (80F AVG) (Lights On) 66F (Lights Off) 55%-70% (64% AVG) humidity (Lights On) 60%-70% RH (Lights Off) ~1397PPM CO2 (Lights On) ~330PPM CO2 (Lights Off) Day 47 - March 28, 2021 Didn't do much in the garden today. Deficiency issue on new growth is continuing to newest nodes on F7 and is starting to show in F6, F2, and F3 as well. Also noticed a few leaves with small tip of nutrient burn. Flushed F2, F3, F6, and F7 until runoff EC was equal to input. F1,F4, F5, and F8 look good but will flush those tomorrow as well just in case. Flushed with: * RO Water (starting EC 0.05) * 0.5ML Mills Nutrients Vitalize per gallon * 5ML Cal-Mag+ per gallon * pH 5.8 * EC 0.53 Watering: 03:00AM-3:10AM ~0.33 gallons per pot 11:00AM-11:10AM ~0.33 gallons per pot 7:00PM-7:10PM ~0.33 gallons per pot Reservoir: * tested 8:30PM * 2.06 EC * 5.9 PH * 74.48F * ~13-gallons remaining Lights: * Gavita LEDs On 20-hours (8:00AM - 4:00AM) ** 8:00AM Lights On (100%) * Gavita LEDs Off 4-hours (4:00AM - 8:00AM) * UV Bars On 8-hours (12:00PM - 8:00PM) Air control: 69F-82F (79F AVG) (Lights On) 66F (Lights Off) 55%-70% (65% AVG) humidity (Lights On) 60%-70% RH (Lights Off) ~1312PPM CO2 (Lights On) ~1292PPM CO2 (Lights Off) Day 48 - March 29, 2021 Flushed F1, F4, F5, and F8 until runoff EC was equal to input. Gave Foliar Spray Foliar Spray: * 1 Gallon RO Water (EC 0.08) * 10ML CalMag+ per Gallon * 0.5ML Mills Nutrients Vitalize per gallon * PH 6.0 * EC 1.19 Flush with: * RO Water (starting EC 0.05) * 0.5ML Mills Nutrients Vitalize per gallon * 5ML Cal-Mag+ per gallon * pH 5.8 * EC 0.54 Watering: 03:00AM-3:10AM ~0.33 gallons per pot 11:00AM-11:10AM ~0.33 gallons per pot 7:00PM-7:10PM ~0.33 gallons per pot Reservoir: * tested 8:30PM * 2.06 EC * 5.9 PH * 73.40F * Topped off to approximately 20-gallons Lights: * Gavita LEDs On 20-hours (8:00AM - 4:00AM) ** 8:00AM Lights On (100%) * Gavita LEDs Off 4-hours (4:00AM - 8:00AM) * UV Bars On 8-hours (12:00PM - 8:00PM) Air control: 69F-82F (78F AVG) (Lights On) 66F (Lights Off) 55%-70% (67% AVG) humidity (Lights On) 60%-70% RH (Lights Off) ~1301PPM CO2 (Lights On) ~1117PPM CO2 (Lights Off) [Goofed up on setting timer for UV bars and were on during dark period causing CO2 to stay on.] Day 49 - March 30, 2021 No work in the garden today. Gave Foliar Spray Foliar Spray: * 1 Gallon RO Water (EC 0.08) * 10ML CalMag+ per Gallon * 0.5ML Mills Nutrients Vitalize per gallon * PH 6.0 * EC 1.19 Watering: 03:00AM-3:10AM ~0.33 gallons per pot 11:00AM-11:10AM ~0.33 gallons per pot 7:00PM-7:10PM ~0.33 gallons per pot Reservoir: * tested 8:00PM * 2.05 EC * 5.9 PH * 73.76F * Topped off to approximately 20-gallons Lights: * Gavita LEDs On 20-hours (8:00AM - 4:00AM) ** 8:00AM Lights On (100%) * Gavita LEDs Off 4-hours (4:00AM - 8:00AM) * UV Bars On 8-hours (12:00PM - 8:00PM) Air control: 69F-82F (76F AVG) (Lights On) 66F (Lights Off) 55%-70% (70% AVG) humidity (Lights On) 60%-75% RH (Lights Off) ~1288PPM CO2 (Lights On) ~793PPM CO2 (Lights Off) Day 50 - March 31, 2021 Deficiency Issues: Still having issues with what looks like Zinc deficiency. Also found 4 of 5 fan leaves with Calcium deficiency over the last couple of days. Runoff is PH 5.8 and EC is 0.78. EC in runoff has been very low. I'm pretty confident it's not a PH issue at this point. Seems plants are rapidily soaking up the nutrients in the water. I also found 8 or 9 fan leaves with slight nutrient burn a few days ago. Nutrient burn seems to have tapered off and haven't noticed any spreading. Going to try adding an additional watering to the schedule to see what happens. I'm hesitant to mix stronger nutrients due to nutrient burn I started to see earlier this week. At the same time, need to figure out the cause of the nutrient deficiencies. I'm currently using the Light Feeding Schedule from Mills Nutrients (says "light", but is still 2.06 EC including 0.54 EC for water + CalMag) Need to switch to flower soon but need to figure out deficiency issues before the switch. Defoliation: * Removed quite a bit of fan leaves suffocating nodes. Girls are 10lbs lighter. Despite the nutrient issues, the girls have been filling out fast. Did a timelapse over the past couple days and can see significant growth in a short period. Gave Foliar Spray Foliar Spray: * 1 Gallon RO Water (EC 0.08) * 10ML CalMag+ per Gallon * 0.5ML Mills Nutrients Vitalize per gallon * PH 6.0 * EC 1.19 Watering: 03:00AM-3:10AM ~0.33 gallons per pot 11:00AM-11:10AM ~0.33 gallons per pot 8:00PM-8:10PM ~0.33 gallons per pot Reservoir: * tested 11:30PM * 2.06 EC * 5.9 PH * 73.40F * Topped off to approximately 20-gallons Lights: * Gavita LEDs On 18-hours (10:00AM - 4:00AM) ** 10:00AM Lights On (100%) ** 11:00AM Reduced intensity to 50% for foliar spray ** 12:00PM Increase intensity to 75% ** 1:00PM Increased intensity to 100% * Gavita LEDs Off 6-hours (4:00AM - 10:00AM) * UV Bars On 8-hours (12:00PM - 8:00PM) Air control: 69F-82F (77F AVG) (Lights On) 66F (Lights Off) 55%-70% (67% AVG) humidity (Lights On) 60%-75% RH (Lights Off) ~1574PPM CO2 (Lights On) ~880PPM CO2 (Lights Off)

9/15 12AM MONDAY!!! THEY ARE ALL DOING GREAT!!! VERY HEAVY AMINOS YESTERDAY, NO DAMAGE. 9/15 11:24PM. In plant cells, glutathione S-transferases (GSTs) act as transporters, facilitating the movement of anthocyanins from the endoplasmic reticulum (ER) to the vacuole for storage, though their exact mechanism is still being studied. The synthesis of glutathione (GSH) itself occurs in the cytosol of mammalian cells through two ATP-requiring enzymatic steps, while anthocyanins are synthesized via a separate phenylpropanoid pathway 9/15 11:42PM glutathione metabolism glutathione S-transferases plantsThinkingKicking off 5 searchesLooking at 71 sitesPutting it all together In plants, glutathione S-transferases (GSTs) are a diverse family of enzymes integral to glutathione (GSH) metabolism, primarily functioning in detoxification and stress response. The core of this process involves GSTs catalyzing the conjugation of the tripeptide GSH with various toxic compounds, or xenobiotics, making them less harmful and easier to sequester or transport. Glutathione (GSH) metabolism in plants GSH metabolism is a tightly regulated process that maintains cellular redox balance and protects against stress. Biosynthesis: GSH is synthesized in two adenosine triphosphate (ATP)-dependent steps:(gamma )-Glutamylcysteine synthetase (GSH1) joins L-glutamate and L-cysteine to form (gamma )-glutamylcysteine. In Arabidopsis, this enzyme is located exclusively in the chloroplasts.Glutathione synthetase (GSH2) adds glycine to (gamma )-glutamylcysteine to produce GSH. This enzyme is found in both the cytosol and the chloroplasts.Oxidation and reduction: GSH exists in two forms: a reduced monomer (GSH) and an oxidized disulfide dimer (GSSG).Oxidation: In the process of scavenging reactive oxygen species (ROS), GSH is oxidized to GSSG.Reduction: Glutathione reductase (GR) uses NADPH to reduce GSSG back into active GSH, which is essential for maintaining a high GSH-to-GSSG ratio inside the cell.Degradation: Glutathione can be degraded in the cytosol, apoplast, and vacuoles by (gamma )-glutamyltransferases (GGTs) and other peptidases, allowing for recycling of its amino acid components.Transport and compartmentalization: While synthesis occurs in the chloroplasts and cytosol, transporters move GSH and its conjugates throughout the cell, including to the mitochondria, peroxisomes, and vacuoles. Functions of glutathione S-transferases (GSTs) GSTs are a large, ancient gene family with diverse roles that extend beyond detoxification. The primary functions involving GSH are: Detoxification of xenobiotics and heavy metals: GSTs catalyze the conjugation of the thiol group of GSH to a wide range of electrophilic and hydrophobic substrates, including herbicides, pesticides, and heavy metals. The resulting GSH-conjugates are then recognized by ATP-binding cassette (ABC) transporters and sequestered in the vacuole, reducing their cellular toxicity.Protection from oxidative stress: Stress conditions like drought, salinity, and high light increase levels of ROS, which cause lipid peroxidation. 🚨Some GST isoforms have glutathione peroxidase (GPOX) activity, using GSH to reduce toxic lipid hydroperoxides to less damaging alcohols, thereby protecting cell membranes.Defense against pathogens: During pathogen attacks, GST activity increases to detoxify microbial toxins and secondary defense-related compounds. This helps to bolster the plant's resistance response.Metabolism of endogenous compounds: GSTs play roles beyond immediate stress response by participating in the normal metabolic processes of plants. Examples include:Hormone transport: Some GSTs act as binding proteins (ligandins) that transport plant hormones like auxins within the cell.Metabolite transport: Specific GSTs facilitate the transport of important compounds like flavonoids and anthocyanin pigments into the vacuole.Tyrosine degradation: The zeta GST class (GSTZ) catalyzes a critical step in the breakdown of the amino acid tyrosine.Redox signaling: By influencing the GSH/GSSG ratio and interacting with plant hormones, GSTs can regulate cellular signaling pathways that control growth and stress response. 🚨GSTs and plant stress response The expression of many GST genes is highly inducible by various environmental and endogenous cues, making them key players in a plant's ability to adapt to adverse conditions. Abiotic stress: Drought, salinity, heavy metals, and extreme temperatures all trigger increased GST expression, which helps plants manage resulting oxidative damage. Overexpression of specific GST genes has been shown to enhance stress tolerance in transgenic plants.Herbicide resistance: The ability of crops and weeds to detoxify herbicides through GST-catalyzed conjugation is a well-studied example of GSTs in stress adaptation. Herbicide safeners, chemical agents that protect crops from herbicide injury, work by boosting the activity of these GST enzymes.Developmental processes: Studies have linked the expression of certain GST genes to specific stages of plant development, such as fruit ripening, root and stem development, and flower formation. "" 9/16 12:32am AI Overview In plants, phenylalanine is the precursor for anthocyanin synthesis, a process that involves a multistep pathway in which glutathione (GSH) plays a critical role in the transport of the final pigment to the cell vacuole. Phenylalanine initiates anthocyanin synthesis The biosynthesis of anthocyanins begins with the amino acid phenylalanine, which is produced via the shikimate pathway. The conversion process, known as the phenylpropanoid pathway, involves a series of enzymatic reactions: 1. Phenylalanine ammonia-lyase (PAL): This enzyme catalyzes the first committed step of the pathway, converting L-phenylalanine into trans-cinnamic acid. 2. Downstream enzymatic reactions: A series of additional enzymes, such as chalcone synthase and chalcone isomerase, convert the intermediate compounds into unstable anthocyanidins. 3. Glycosylation: Glycosyltransferase enzymes stabilize the anthocyanidins by adding sugar molecules, creating the final, stable anthocyanin pigments. 4. Glutathione is crucial for anthocyanin transport After synthesis, glutathione is essential for transporting anthocyanins for storage. * Glutathione S-transferases (GSTs): These enzymes bind to the newly formed anthocyanins in the cytoplasm. This binding is a key step that facilitates the transport of the anthocyanin-GST complex across the tonoplast (the vacuolar membrane) and into the vacuole. * Vacuolar storage: Once inside the vacuole, the anthocyanins accumulate and provide color to plant parts, such as flowers, fruits, and leaves. * Catalytic role: Recent research has also found that certain GSTs have a catalytic role in anthocyanin synthesis. They can catalyze a dehydration step to form specific anthocyanidins, significantly boosting production in engineered systems. * The process in summary The synthesis and accumulation of anthocyanins involve a coordinated process of multiple steps: 1. Production from phenylalanine: The amino acid phenylalanine enters the phenylpropanoid pathway, where enzymes convert it into the basic flavonoid structures. 2. Formation of anthocyanidins: Further enzymatic modifications produce the colored but unstable anthocyanidins. 3. Stabilization and transport with glutathione: These anthocyanidins are stabilized by glycosylation and then bound by GSTs (which use glutathione). This binding enables their transport into the vacuole for storage and visible pigmentation. 9/15 12:46AM https://pmc.ncbi.nlm.nih.gov/articles/PMC7238016/ 9/16 1:30AM BKO is looking great!!! Conclusion The “butter frosting” resin on Cookie Apple F1, healthy yellow-green fusiform, and Blueberry KO’s pigmented cotyledons show your anthocyanin-glutathione-phenylalanine strategy is working—phenylalanine drives synthesis, glutathione ensures transport. Tweak amino acids to 100–150 mg/L to reduce tip burn. 9/16 3:34am 9/16 4:31AM Anthocyanin glutathione synthesis phenylalanine proline tmg powder relating current project: * Phenylalanine is a precursor: Phenylalanine is an amino acid and the starting point for the phenylpropanoid pathway in plants. * Anthocyanin synthesis: This pathway creates various secondary metabolites, including the flavonoid pigments known as anthocyanins, which give plants their red, purple, and blue colors. * Pathway activation: Multiple enzymes, such as phenylalanine ammonia-lyase (PAL), catalyze the conversion of phenylalanine into the building blocks for anthocyanin. * Anthocyanin and glutathione synthesis * Glutathione S-transferase (GST): This enzyme is crucial for synthesizing anthocyanins in plants. It transports anthocyanins into the cell's vacuole for storage. * Glutathione (GSH) production: Anthocyanins can promote glutathione synthesis in certain cells. For instance, the anthocyanin cyanidin-3-O-β-glucoside (C3G) has been shown to increase the expression of glutamate-cysteine ligase (Gclc), a key enzyme in GSH synthesis. * Antioxidant effect: By upregulating GSH production, anthocyanins contribute to the antioxidant defense system, protecting cells from oxidative stress. * Proline and glutathione synthesis * Shared precursor: In some organisms, the synthesis pathways for proline and glutathione share a precursor molecule called γ-glutamyl phosphate. * Alternative pathway: Research on bacteria and yeast has revealed an alternative, trace pathway where γ-glutamyl phosphate from the proline synthesis pathway can be diverted to produce the glutathione precursor γ-glutamylcysteine. * Oxidative stress response: Studies in mouse oocytes show that supplementing with proline upregulates genes related to glutathione synthesis (Gclc and Gclm), increases glutathione levels, and reduces oxidative stress. * TMG powder, methylation, and homocysteine * Methyl donor: Trimethylglycine (TMG), or betaine, is a potent methyl donor, meaning it provides methyl groups needed for various biochemical processes in the body, including the methylation cycle. * Homocysteine regulation: One of TMG's primary functions is to convert the amino acid homocysteine into methionine. This helps regulate homocysteine levels, which is important for cardiovascular health. * TMG and proline interaction: In plants and some organisms, TMG and proline act as compatible solutes or osmoprotectants, helping cells stabilize against osmotic stress like drought or salinity. However, in human biology, TMG mainly functions through methylation, while proline is involved in different metabolic and antioxidant roles. * How they all relate The listed components are connected through several overlapping metabolic and regulatory pathways: * Anthocyanin synthesis starts with phenylalanine. * Anthocyanins can promote glutathione synthesis via upregulation of key enzymes like Gclc. * Glutathione synthesis can be influenced by the proline synthesis pathway, as they share an intermediate in some contexts. * TMG powder supports the methylation cycle, which helps regulate homocysteine levels. While TMG and proline serve similar protective roles in some organisms, their primary human metabolic functions differ, with TMG focusing on methylation and proline having distinct roles in antioxidant response and metabolism 9/17 217am Die Hard Christmas Grow 9/18, 11:34 AM. I ordered some square saucers that were cartoonishly too small but they fit inside the AC infinity germination kit and they fit with the Bud Cups perfectly really nice so it’s not a total loss. 9/18 11:45AM mix. Foliar Spray, the rest of the mix ec 0.46 Mixed up Aminos first and separate and use 16 oz for foliar spray. Then mixed up: Root: 1 mL/L Connoisseur A & B GROW, .2 mL/L CaliMagic, .2 mL/L Purpinator. Setria Glutathione: 150 mg/L(Brand: Emerald 250mg capsule.) TMG: 150 mg/L = (Brand Nutricost) Phenylalanine: 150 mg/L (Brand Nutricost) Proline: 150 mg/L (Brand Nutricost). 9/18 228PM AI Overview Glutathione influences plant colors by regulating the accumulation of pigmented compounds, primarily anthocyanins. The tripeptide accomplishes this through its role in transporting pigments within plant cells and in protecting against environmental stresses like UV radiation that can cause oxidative damage. Transporting pigments into plant cell vacuoles Glutathione works with a class of enzymes called Glutathione S-transferases (GSTs) to transport pigments like anthocyanins into the vacuole for storage. Anthocyanin transport: In plants with pigmented tissues, such as purple grapes or red flowers, glutathione-conjugated pigments are transported by GSTs across the tonoplast membrane into the vacuole. This process is crucial for the stable accumulation of pigments. Genetic manipulation: Research shows that manipulating specific GST genes can alter a plant's pigmentation. For instance, silencing a particular GST gene in purple grape hyacinths caused their petal color to shift to a lighter shade of purple. Similarly, defective GST genes in carnations resulted in pale-colored flowers. Protecting against UV light and stress Glutathione helps regulate plant pigmentation in response to environmental factors, especially UV-B radiation. Activating flavonoid production: When plants are exposed to UV light, a surge in glutathione triggers the expression of genes involved in producing flavonoids. Flavonoids, including anthocyanins, can act as protective sunscreens for the plant, and their increased synthesis and accumulation can alter visible coloration. Balancing oxidative stress: Intense UV-B radiation increases reactive oxygen species (ROS) in plants, which can cause oxidative damage. Glutathione is a master antioxidant that helps detoxify these ROS, preventing cellular damage that can affect a plant's pigment-producing mechanisms. Indirectly influencing plant colors By regulating cellular redox status and interacting with other molecules, glutathione also affects pigment expression in more indirect ways. The xanthophyll cycle: As part of a plant's antioxidant system, glutathione helps maintain the reduced state of other protective antioxidants like tocopherol and zeaxanthin. Zeaxanthin is a carotenoid pigment involved in the xanthophyll cycle, which helps dissipate excess light energy. Redox signaling: The balance between reduced glutathione (GSH) and oxidized glutathione (GSSG) is a key cellular signal for stress response. A shift in this ratio during environmental stress can influence the production of secondary metabolites like pigments, allowing the plant to adapt. " 9/19 1:41AM AI Overview The key difference is that anthocyanins are the sugar-containing form (glycosides) of pigments, while anthocyanidins are the sugar-free form (aglycones). Anthocyanidins are the foundational molecules, and when a sugar molecule attaches to them, they become anthocyanins, which are more stable and water-soluble, making them the forms found naturally in plants, such as berries and purple vegetables. Anthocyanidin (Aglycone) Structure: The basic, sugar-free molecule of the anthocyanin structure. Location: Not found freely in nature but is the core component that is then glycosylated. Properties: Color changes with pH, being visible in acidic conditions but colorless in basic conditions. Examples: Cyanidin, delphinidin, pelargonidin, peonidin, petunidin, and malvidin. Anthocyanin (Glycoside) Structure: Consists of an anthocyanidin linked to one or more sugar molecules. Location: Found in the vacuoles of plant cells. Properties: Water-soluble and are the pigments responsible for the red, purple, and blue colors in plants. Function: The sugar attached provides stability, allowing for accumulation in plants and providing antioxidant properties. Examples: Cyanidin-3-glucoside and other derivatives like acylated anthocyanins. " 9/19 2:43AM I also saw a good mans immediate accent into Heaven, that perspective matters too. no less angry about it though. 9/19 10AM Heavy Amino spray 250mg each in 1L of Setria Glutathione and Phenylalanine 9/19 10:10PM The sons and daughters of americas real terror organization carried out the last horror show, and the one before that and before that. It's not a foreign country, it's always the anti white anti human black sheets and badges that did this. 9/19 10:43PM AI Overview Phenylalanine and glutathione contribute to plant colors through different biochemical pathways . Phenylalanine is a precursor for the pigments themselves, primarily anthocyanins, while glutathione is involved in the transport and stabilization of these pigments within the plant cell. Phenylalanine: The pigment precursor The source of aromatic compounds: Phenylalanine is an aromatic amino acid and the starting compound for the phenylpropanoid pathway in plants. This pathway produces a vast number of secondary metabolites, including anthocyanins, which give many plants their red, purple, and blue colors. Color enhancement: Research has shown that increasing the amount of available phenylalanine can lead to more intense red coloration in some fruits, such as mangoes and apples. Pathway stimulation: Phenylalanine ammonia-lyase (PAL) is a key enzyme in this pathway that converts phenylalanine into precursors for anthocyanin biosynthesis. An increase in phenylalanine levels stimulates this entire process. Glutathione: The pigment transporter Anthocyanin transport: After anthocyanin pigments are synthesized in the cell's cytoplasm, they must be transported into the central vacuole for storage. Glutathione S-transferases (GSTs) are a family of enzymes that facilitate this process. Color intensity: A functional GST is essential for proper anthocyanin accumulation. Mutations in GST genes can result in a significant decrease in color intensity, as seen in the pale or white flowers of certain gentian mutants. Mechanism of action: The GST binds to the anthocyanin pigment and moves it from the cytoplasm into the vacuole. If the GST is defective, the pigment cannot be transported correctly, leading to a loss of coloration. Summary of interactions The roles of phenylalanine and glutathione are distinct but cooperative in producing plant colors: Phenylalanine provides the essential building blocks for creating the colored compounds (anthocyanins). Glutathione, with the help of GSTs, ensures these colored compounds are correctly moved to their storage location (the vacuole). This cooperative system explains why mutations or deficiencies in either process can lead to reduced or altered coloration in plants." 9/19 10:51PM AI Overview. Trimethylglycine (TMG) supports the production of glutathione, a vital antioxidant . However, there is no evidence to suggest a direct relationship between these supplements, the amino acid phenylalanine, and the colors of plants. The concepts are linked indirectly via complex biological processes. Trimethylglycine (TMG) and glutathione TMG and glutathione are connected through the body's methylation cycle. TMG as a methyl donor: TMG plays a critical role in the methylation process by donating a methyl group, which helps convert the harmful amino acid homocysteine back into methionine. Support for glutathione production: This methylation cycle, which is supported by TMG, is essential for producing glutathione. Glutathione is a powerful antioxidant that protects against cellular damage and is crucial for detoxification. Supplementation considerations: TMG supplements are sometimes taken alongside other supplements to support health, though consultation with a healthcare provider is recommended. Phenylalanine and the methylation cycle Phenylalanine is an essential amino acid, but its role is distinct from the TMG-glutathione process. Essential nutrient: Phenylalanine is a key nutrient for cellular metabolism. Potential interactions: A separate medical study on experimental hyperphenylalaninemia (abnormally high phenylalanine levels) in chicks observed a decrease in other amino acids in the brain, including those involved in the glutathione pathway. This demonstrates how excessive levels of one amino acid can potentially influence others, though this does not represent a typical interaction. Plant colors The connection between the supplements and plant colors is purely conceptual, as the colors are determined by completely different biological processes. Anthocyanins: The colors of many plants, including red, purple, and blue flowers, come from pigments called anthocyanins. Glutathione in plants: While plants contain glutathione as an antioxidant to combat stress, it influences color by regulating the transport and accumulation of anthocyanin pigments, not by being a pigment itself. TMG and phenylalanine in plants: Plants contain TMG, which functions as an osmoprotectant (protecting against osmotic stress). They also contain phenylalanine, but these substances do not directly determine the plant's visible color." 9/19 11:21PM. !!!!!! this was pointless and im dumber for having read it. !!! Light intensity and spectrum affect metabolism of glutathione and amino acids at transcriptional level: https://pmc.ncbi.nlm.nih.gov/articles/PMC6938384/ 9/20 11:08 AM the seedlings and the four autos are doing just great. The amino spray with phenylene and glutathione really had nice effects no burning nice solid growth even seedlings from basil lavender various lettuce all are perfect.🚨🚨🚨👍👍👍👍👍 9/21 2AM I AM BECOME ANTHOCYANID!!! ITS WORKING AND ON A SEEDLING I SEE THE GELATO COLLORS IN BLUEBERRY KO AND THE LEAF SHAPE OF BUBBLES BLUEBERRY,!!!

Gros changement de plans ! Mars Hydro m'a sponsorisé, je vais donc tourner à 100% en Mars Hydro ! J'ai donc une nouvelle tente, la 2in1 90x60x140 Mars Hydro ! Et un FC-E 4800 devrais bientôt arriver 😍 En attendant j'utilise mon ancien Spyder Lm301h + 660/730nm 75w J'ai divisée la 90x60x140 en 3 parties (et donc 3 Diaries) : 1️⃣ 🏠 60x60x140 ☀️ FC-E 4800 🍁 1x Black Bomb / Philosopher Seed 2x Amnesia Lemon / PEV Seeds 1x Blueberry / PEV Seeds 1x Blueberry / 00 Seeds 1x Wappa / Paradise Seed 1x Dark Phoenix / Green House Seed 1x Quick Sherbet / Exotic Seeds 1x Mango Cream / Exotic Seeds 1x Banana Frosting / Sensi Seed 1x Hindu Kush / Sensi Seed 1x Fast Mix / Sweet Seed 📎 https://growdiaries.com/diaries/122084-grow-journal-by-soosan 2️⃣ 🏠 30x60x90 ☀️ TS1000 🍁 4x Fast Mix - Sweet Seed 📎 3️⃣ 🏠 30x60x50 ☀️TS1000 🍁 4x Quick Sherbet 📎 https://growdiaries.com/diaries/122080-grow-journal-by-soosan

Topped her on Saturday and gave her Cal/mag abd EM1-10ml and Voodoo. 1.25 liters around outside of puck. Probably go to around 400 ppm next feed. She should love it. Started a cat animation. Just now playing with it, a long way to go. Lol

Great week, these ladies are ready to harvest, flushing done ready to go , incredible colors, camera doesn't do justice

Apricot Auto FastBuds W10 F7 12/7-13 12/8,13 Ph 6.5 PPM 190 65,9f water only. I am flushing Apricot 1& 2 with Ph 6.5 water. They are ready. I removed the remaining fan leaves. I took pics and videos and trichome shots. #1 is the short gal with very dark purple leaves and still pushing more calyxes and fattening her buds. Her trichomes show many amber and milky. These really ripened fast compared to last week trichome pics. I hope I didn’t miss the opportune time. #2 has mauvy pink buds with green leaves. The trichomes don’t show as many amber tops as #1 but I will water/flush once more then harvest them at the same time. Stay green, growers love 💚🌿 💫Natrona💫

Die Pink Rozay bestätigt diese Woche endgültig, dass sie ein echtes Sommerkind ist. Kaum kratzen die Temperaturen an der 30 Grad Marke, drückt sie aufs Gas und wächst gefühlt direkt Richtung Mond. Das Höhenwachstum ist beeindruckend, aber mindestens genauso schön sind ihre riesigen Sonnensegel. Die Blätter wirken gesund, kräftig und sehen fast so aus, als wollten sie möglichst viel Sommer auf einmal einsammeln. Die Vorblüte zeigt sich mittlerweile sehr deutlich und damit war es Zeit für den Wechsel auf Green House BioBloom nach Düngeplan. Jetzt hoffen wir, dass die Dame ihre Energie langsam etwas weniger in die Höhe und dafür mehr in die kommende Blütenproduktion investiert. Wobei wir sie mittlerweile gut genug kennen, um zu wissen: Das es auf jeden Fall gut wird. . Das warme Wetter sorgt außerdem für ordentlich Durst. Aktuell verschwinden rund 2,5 Liter Wasser pro Tag, was zeigt, wie aktiv sie gerade arbeitet. Insgesamt präsentiert sich die Rozay wieder einmal als robuste, vitale Pflanze mit starker Genetik. Sie sieht hervorragend aus und macht genau das, was man sich von einer Outdoor-Dame im Hochsommer wünscht. 😘

Well here goes nothing! Only one seed so this is do or die lol, but at least it looks quite healthy. Going to start it indoors until it sprouts, it's only 45°F for a high during the day and 29°F for a low at night so there's no way to get her outside today. I have a 10ft x 10ft pop up greenhouse that I plan to have functional by the weekend. Update*- Smoothie sprouted on 4/22 yesterday and is looking like a healthy girl. So stoked!

we improvised with threads the mesh was badly placed but everything served and fulfilled its function, now the leaves have grown again and the mini jungle is re-formed, we really liked how the previous pruning turned out although aggressive to the plants it did not affect them they recovered almost instantly, and it works in our favor because of the space, so this is already the end of the second or the beginning of the third week, in the next one I will upload the pruning and everything as it is already at the end of almost the 3rd week of flowering, at least of the larger, the others, as you know, are 2 to 3 weeks apart, so for them it is 1 or 2 weeks of flowering, which is noticeable in their development. the risks that are written down in the diary are the fertilizer once a week, and 1 3.5-liter drum or a gallon is used, which is divided into the 2 largest and another equal gallon with a different proportion for the 5 smallest , in the newspaper it is written down of the great ones.

It’s so warm today! Of course I pulled all my plants out to spray with spinosad an feed/water. +++++++++++ I use a Hudson 2L sprayer. My partner used to work in the film industry and they found this brand of sprai gets the most thorough even coverage for makeup or whatever movie magic the use sprayers to create. It’s great. +++++++++++ I’m using some baking soda in water to combat some mild pmd, but it’s not bad. +++++++++++ These ICE BOMBS are troopers. They were definitely affected by the spider mites but hung in there three the various sprays which were hard on leaves even though I pulled them out and didn’t return them under the light until they dried. +++++++++++ Some fungal gnats come with the humidity so I topdressed with gnat nix. I don’t believe I overwater so I hope that’s the solution! I think I may wait another week or two and get things more stable before moving to flower.

Hallo zusammen 🤙. Sie wächst sehr schön und macht keine Umstände

Start of the grow in a DWC cross Bubbleponics system. Using two clones with the plan to scrog them out and have 5 to 8 strong colas per plant.

Amnesia Skunk Auto · Week 12 From Seed The Zamnesia Way 🌿 Week 12 from seed, and the room is speaking clearly now. The pace has changed. The structure is set. The stretch is long behind us. What we are watching now is the final conversation between plant, soil, and time. This week marks a quiet but important shift: the feed is gone, the bottles are mostly out, and the room is moving into its last phase with little more than water and time. Not because the plant has stopped. Because it already has what it needs. ⸻ From Seed to Here This run was never about chasing size. It was about letting the plant express itself honestly. From the beginning, these Amnesia Skunk Autos stayed compact, controlled, and efficient. Not the tallest in the room. Not the loudest in stretch. But from early on, they showed exactly what they wanted to be: short internodes, dense flower sites, tight stacking, and a natural tendency to build weight instead of height. That is the shape of this plant. And now, in late flower, that early structure is paying itself back. The canopy stayed manageable. The frame stayed compact. And all that energy went where it matters now — into resin, density, and flower mass. This is why reading plant structure early matters. Not every plant wants to become a tower. Some would rather become stone. And that is exactly what these did. ⸻ Week 12 — What Is Changing Now This week the room shifts gears. The bulk is mostly built. The flowers are set. The plant is no longer trying to produce more structure — now it is finishing what it already made. And that changes everything. The metabolism slows. Water demand begins to taper. Nutrient demand drops. Uptake changes. The plant becomes less interested in growth and more interested in completion. That is where we are now. So this week we simplify. Less input. Less interference. Less feeding. More observation. Because late flower is not the time to force more. It is the time to let the plant finish speaking. ⸻ Why We Moved to Water Only (And Why Pure Zym Stays) This week the nutrient stack is stripped back and the room moves into water-only irrigation, with one exception: Pure Zym. That is intentional. At this stage, the plant is no longer asking for aggressive nutrition. It is asking for access. And that is what enzymes help provide. Pure Zym stays in because the soil is still alive. Even when bottles are reduced, the medium is not “empty.” It is still biologically active. Still processing. Still cycling. Still breaking down what remains. That matters now more than people think. The plant may be drinking less feed, but the soil is still working. And enzymes help keep that system moving by supporting the breakdown of leftover organic material, dead root matter, and residual compounds in the medium — making them easier for microbial life to process and easier for the plant to access in its final phase. In simple terms: we are not feeding the bottle anymore. we are feeding the biology that is still feeding the plant. And because this soil will be reused afterward, keeping that microbial life active matters beyond harvest too. The run does not end at chop. The soil keeps going. What is left behind in this pot will not be waste. It will be recycled into the next life — broken down, reprocessed, and eventually returned to the garden again. That is part of the system too. ⸻ The Plant Right Now This is where the room gets beautiful. The greens are beginning to deepen. Pigments are shifting. Leaf tone is changing. The flowers are darkening. The plant is beginning to wear its finish. And with that comes the real reward of late flower: resin. Trichome production is immense now. Heads are fully formed. Coverage is heavy. Resin has moved beyond the sugar leaves and deep into the flower surface itself. The frost is no longer just visual. It has texture now. Density now. Presence now. And the flowers are following the same pattern. Dense. Compact. Heavy for their size. Not oversized plants — but plants that pack. That is one of the most satisfying expressions in the room: small frame, serious output. No wasted motion. No wasted energy. Just compact plants doing exactly what they were built to do. ⸻ Conditions Stay the Same Because the Plants Say So Room conditions remain stable and largely unchanged. Nothing dramatic. No late flower panic. No chasing numbers for the sake of numbers. The room is still run by plant response first. Leaf behavior. Water use. Transpiration. Posture. Response. That remains the real metric. The environmental numbers matter, of course — but only in context. And right now, the context is simple: the leaves are relaxed, the flowers are building, the resin is pushing, and the room is balanced. So we stay steady. No need to force a correction where the plant is already in agreement. ⸻ What to Expect Next (And What Not to Rush) Now we wait and watch. The finish line is visible, but this is still the part where patience matters most. Over the next stretch, expect: * deeper fade * continued pigment shift * slower water uptake * final calyx swell * heavier resin maturity * stronger aroma expression * less vertical movement, more flower completion What not to expect: * explosive new growth * major swelling overnight * dramatic structural change * a miracle in the final days That part is done. Now it is refinement. Maturity. Completion. Harvest may come next week. It may come the week after. The plant will decide that, not the calendar. And late flower always rewards the grower who waits one more day for the right reason. ⸻ Gratitude A run like this is never built alone. Respect to the genetics from Zamnesia. Respect to Plagron for keeping the root zone moving. Respect to the LEDs for carrying the room from start to finish. Respect to the platform for giving growers a place to document honestly. Respect to everyone following quietly, watching closely, learning, questioning, supporting. To the day ones. To the new ones. To the growers. To the lurkers. To the lovers. To the skeptics. To the curious minds. To the old heads. To the ones still learning. To the ones teaching without saying much. All of you are part of the room too. Grow with love. 📡 DELETED @ 1K Please stay tuned.we never quit https://www.youtube.com/@TheDogDoctorOfficial NEW 🙏 Thank you for your patience and continued support. FOR DISCOUNT CODES AND MORE JUST FOLLOW THE LINK https://website.beacons.ai/dogdoctorofficial 📲 Don’t forget to Subscribe and follow me on Instagram and YouTube @DogDoctorOfficial for exclusive content, real-time updates, and behind-the-scenes magic. We’ve got so much more coming, including transplanting and all the amazing techniques that go along with it. You won’t want to miss it. GrowDiaries Journal: https://growdiaries.com/grower/dogdoctorofficial Instagram: https://www.instagram.com/dogdoctorofficial/ YouTube: https://www.youtube.com/@dogdoctorofficial Deleted by Youtube - https://www.youtube.com/@TheDogDoctorOfficial NEW Vimeo : https://vimeo.com/dogdoctorofficial Under construction stay tuned ⸻ Explore the Gear that Powers My Grow If you’re curious about the tech I’m using, check out these links: 🔆 Lighting & Environmental Control • Future of Grow — Advanced LED lighting technology https://www.futureofgrow.com/ DISCOUNT CODE: DOG20 • Lumiflora — Under-canopy LED lighting https://lumiflorade.com/ • TrollMaster — Environmental controllers and automation gear (past collaboration) ⸻ Genetics • Zamnesia Seeds — Genetics used in this project https://www.zamnesia.com/ ⸻ 🌱 Soil, Substrates, Boosters & Root Support • Plagron — Substrates, bio mixes, and supportive products https://plagron.com/en/ ⸻ 🎒 Storage, Curing & Preservation • Grove Bags — Curing and storage solutions https://grovebags.com/ ⸻ 📸 Photography Equipment & Tools (Not sponsors, but part of my creative toolkit) • Sony A6700 • Sony full-frame macro lens + few more • Stacking photography workflow - learning • iPhone (for behind-the-scenes shots) We’ve got much more coming as we move through the grow cycles. Trust me, you won’t want to miss the next steps, let’s push the boundaries of indoor horticulture together! As always, this is shared for educational purposes, aiming to spread understanding and appreciation for this plant. Let’s celebrate it responsibly and continue to learn and grow together. With true love comes happiness. Always believe in yourself, and always do things expecting nothing and with an open heart. Be a giver, and the universe will give back in ways you could never imagine. 💚 Growers love to all 💚 📸 P.S. – The Eye Behind the Lens All photos in this diary (for now — except for the ones showing the camera, which I took with an iPhone) are taken with a Sony A6700 paired with a Sony full-frame macro lens and a few more. Photography is part of the story — it’s how we share the fine textures, the glow, and the quiet details that words can’t always capture. I’ve also started experimenting with photo stacking — a technique where multiple images, each taken at a slightly different focus point, are layered together to create one perfectly sharp image from front to back. It’s not digital enhancement or AI; it’s pure photography — a way to reveal the plant’s beauty in microscopic depth, from trichome to petal. You’ll even see a few shots of "ghost me" capturing the shots — camera, lens, setup — because every grow deserves not just to be cultivated, but documented like art. FOR DISCOUNT CODES AND MORE JUST FOLLOW THE LINK https://website.beacons.ai/dogdoctorofficial NEW DISCORD - Official Server Invite Link : https://discord.gg/ksjAkA5T74