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This week is another step towards the end goal of getting to see these beautifull buds. Gave them a dose of build a flower on day 17. Gave them a heavy dose of molasses on day 21 mild on 17 with flower. 1 pheno is getting covered in resin early. All have a musky gas smell as of now. Something sweet them fist open the tent.
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@Andres
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she is growing healthy ... in her fourth week ... outdoors she has grown in form ... moderately ... and just waiting for her to show her great potential.......
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@BudBeezy
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This week I decided to top the plant. It's the first time I've topped a plant and applied LST. It hurt to just cut the plant down but hopefully it will be worth it in the end. It tolerated it well. I have tried to make cuttings from the topped part. We'll see if they make it. The amount of watering remains unchanged for now as the soil stores enough water. I will water every 3 days from now on. This week I used the nettle manure in a mix with rainwater in a ratio of 1:20. Unfortunately, the weather has got a bit worse this week. The daytime temperature fluctuated between 18 and 21 degrees.
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@Valedor
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semana de crecimiento de tricomas, ademas las flores presentan un crecimiento muy bueno, ya están madurando de verdad, el próximo cultivo lo documentaré con fotos mas claras, he aprendido mucho de tenerlas en hidroponia, me gusta y lo voy a seguir usando y explotando
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@Hawkbo
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Day late on the update, pics/vids were taken on time tho just been a crazy week of harvests and trimming and keeping up with these. I linked up with Rain Science Grow Bags on Instagram and got them to offer all my followers and friends a discount of 10% off entire order from their site with the code ' bangdang ' so if anyone is in the market for a pot upgrade use that code. I got them in the mail 3 days after I ordered. Reason I went with Rain Science is because they offer identical air flow for rapid growth as the radicle bags, just using a different material and a tighter knit so water doesnt flood out the sides during feeds and when you pick these up when the coco is dry, it wont fly all over the tent like with the radicles. They're the optimal bag for autoflowers especially. These 2 are doing great should get interesting in the next few weeks. I actually ran 3 of these in 1 gallon pots a few months ago and 2 atleast came out pretty good so the bus shots are from the 2nd pheno of those.
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@Kushizlez
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Day 56-63 (Day 57) I might have to fix the light to the roof of the tent so I can get another 3 inches up. The ppfd is just too intense at this height. The node stacking is super tight, I’m seeing some droop on almost every plant and the plants just look stunted. I might have to take out the big shelf I just paid 90$ for and raise the light to get the ppfd level down. This happened my last grow as well. It’s a great light for flowering but sucks ass for veg unless it’s hung 5’ above the canopy. I’ve seen videos of others with the same light and they have to fix it to the roof. The dimming function only works with a master controller I don’t have the money for right now. Pulling bbb #2. I’m seeing very small but clear pollen sacks. It’s also drooping like crazy. The roots don’t look as healthy as some of the other males I pulled earlier. They have a slight brown tinge but still mostly white. I wonder if I accidentally gave top dressed it too many times when I mixed them all up a while ago. I’m going to transplant bbb’s #1, #3, #6 & #7 into my 10 gallon destiny/coco pots lightly water. This is kind of a gamble because 2 of them are not showing definitively. Although I’m about 75% sure they’re fems. If not, I got my two backups. (Day 58) I was reading about symptoms of high PH and I definitely have it indicated by the twisted and stunted growth on top. I’m going to try to water in a full gallon at 5.8 and see if I get a good response. I sprayed the pump nozzle directly into the soil after it was empty to put some oxygen into that big 10g pot. The blackberry I gave the lowered PH water too noticeably responded within a few hours. The clawing has pretty much stopped and it now looks like the healthiest plant in the tent. I’m going to see how it responds over the next few days then try it with my other plants if everything looks good. I’m going to try it with bbb #5 in the 1.7g pot and see if it responds in the same way. I ran a gallon of 5.8 water through 1.7g and got less than a 100ml of runoff which is a little surprising. The runoff measured 1750ppms which seems a little on the high side but isn’t insane for peat based soil. (Day 59) Wow. The plants responded to that little PH adjustment insanely well. #5 isn’t dropping at all and the leaf wrinkles are actually flattening out. It’s starting to look like one of the healthiest plants in the tent. I’m still hoping for #5 to show female. 5 plants would cut my veg time down and give me another opportunity for a keeper pheno. All 4 plants are looking like they’ve already recovered from their transplant. #1 in particular was drooping like crazy yesterday and today it’s bounced back and looking happier than ever. I’m seeing a bit of tip droop on a few plants but they still seem fine I wonder if because my soil is 50% coco I should try watering on the acidic side, around 6.0-6.2 from now on. I’m almost certain that solved all the issues I was having. (Day 62) Plants are all looking really healthy. They all seem to have outgrown the initial light stress actually but node stacking is still really tight and I hope to raise the light up next week sometime. I’m going to drop the humidity to 60% for the rest of veg. These pots are massive and I’m still a little worried about dry downs but they aren’t showing any signs of overwatering or lack of oxygen. BBB#3 is starting to show some weird discoloring like an mag or nitrogen deficiency. Which is weird because it’s freshly transplanted into nitrogen rich soil. Guess I will give it a kelp foliar spray. I’ve been looking for a good organic source of K without an absurd amount of magnesium and sulfur like langbeinite has. I found some 0-0-15 kelp/seaweed extract in soluble crystal form. So from time to time I will water that in at half strength or with compost teas to keep my soil K dominant. (Day 63) I’m still waiting on #6 to show sex. I am shocked it hasn’t shown sex yet. Although it was started a week and a half after the others but still. I’m going to be so pissed if it ends up being a male. I’m taking a gamble but I’m confident it will show female when the time comes. bbb#3 got a little more color back from that foliar spray. Hope that doesn’t get worse because it’s my favorite pheno so far. I will give a N boost in the form of compost tea tomorrow afternoon to both of my tents. I’m starting to utilize the scrog net and boy does it make training way easier. This seems to be a much smarter style of growing for yield and quality. I’m getting tired of these pH fluctuations every time I water. I’ve been eyeballing for the most part and I need a consistent formula. I found the perfect mix to stabilize my RO water and stabilize the pH at 6.2. I moistened the top soil of each plant and will watch for any reactions. Water recipe: 60ml bottled water 2000ml RO water 3ml calmag 2ml microbes
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This week has been great progress on the plants, as u can see, giving your plants the oxygen it needs and they will flourish I wish I've gotten a better pump from the beginning, but o well learning curve lol, it has been getting warmer now that spring is arriving, the inside of my tent is at 80° so the only issue I see in the future is water temp. rising but ama try to keep her as cool as possible. (Not running a chiller) I did started to see some pistols coming out so, she starting to flower. Ama switch her nutrients to bloom A&B next week as with some Nirvana see how that goes. Am still checking the pH twice a week (Once in the morning, and again late afternoon.)
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Week 0: Germinated her the old-school way...two paper towels, between 2 saucers.. Fastbuds: I was so impressed with the packaging ..even though I was not able to get the original packaging (Discreet).. Week 1: just sprinkled H20 on her.. Midweek she sprouted. Week 2: Added some AgriSil K50 for her root development..She showed her first true leafs like yesterday..
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@AutoCrazy
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Week 11 Week 4 of Flower This lady is pretty happy right now. She did get a bit of tip burn this week but nothing too serious. This plant really stretched out. Even more so than her tent mate the Mimosa EVO. There are loads of bud site now so the fun has truly begun. I am excited for the weeks ahead. Cooler internals: 5.9pH 650ppm 67F solution temperature Onward 😎🍻
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@Prof_Weed
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9.Vegetationswoche, nun 50 cm hoch und 85 cm breit. Bis 15.3.hoffe ich auf 60 cm Höhe und dann wird auf 12/12 umgestellt. Musste nun schon das 5.Mal entlauben, das wächst wie Unkraut. Seit 3 Tagen benutze ich den WaterTrim, Osmose Wasser.
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@Sunofa420
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Showing lots of white hairs on the bud sites it’s alive
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@Mimi420
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Hi guys! My two Kushes are growing great!I lollipoped them al litle,and started to remove the leafs and branches under the net.And I toped all the highest tops.One or two weeks of veg to go.Stay green!
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-Sensi Seeds Research: The Sensi Seeds Research breeding project has created eleven cannabis seed varieties. How? By combining new cannabis cultivars with a selection of strains from their long-established cannabis gene bank. For the first time in thirty-six years, they are opening the doors of the Sensi Seeds Research and Development Department. Week #15, week #7 of Flowering. Fade is coming out, plants are near to the end of their life cycle. This where new growers start to panic, thinking their ladies are having deficiencies... but no panic ! It’s organic ! Everything is normal 😉 -ScrOG II: #741 , #1318 , Shiva Skunk are ready and will be flushed at the beginning of next week ( tomorrow ). -ScrOG I: #11, Northern Lights, Black Domina, Super Skunk are good for 9 weeks of Flowering, perhaps more, and aren’t ready yet ! I have stopped the Alga-Max ( remember to always stop any P-K Boosters 2 weeks before harvest ) for the ScrOG II since last week and have given the last dose at the beginning of the week to ScrOG I. I must recognize that I’m really fan of the Mars-Hydro SP 250, I can’t wait to see how it works from seedlings to harvest 😎 I would like to finish this week by saying thanks to the brother or brothers who has increased the views on this week, I was wondering what was happening 😄 After discovering their was a contest on the most views of the week 😂😂😂 I knew something was not normal... hahaha so funny 🤣 A BIG THANKS 👍😄 ( I’m looking for a job in the Cannabis industry as Master Grower, Mineralogist, Quality Control 🐞)
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Second week, The leaves are wider, and the plant is taller than last week, I'm going to start the apical cut. The odor is kinda mild but more intense. EC 1.5 PH 5.8 Floraflex 6ml Louder Powder Grow A & B
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@Mazgoth
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The color is amazing,we are talking about a real purple color,the smell is so sweet and smells like a banana tree with milk and fruits.You need a good carbon filter for this strain
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@Budhunter
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As I did the flushing in day 68 I was watching the trichomes and on day 87 I could see 25-35% amber so I decided to cut it. I cut the whole plant on the base and hang them all to dry. For this reason I will update my findings later when it gets dry and trimmed. So far really happy with the results. It won’t yield much but the quality is impressive
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ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.