Hey folks. No new updates really. still on cruise control. Plants are crazy thirsty. Using 10 gal every 2 days or so. So that ends up at 1.2 gal per plant per day! Insane. These air pots do thoer job as advertised. Next update we are going to amend these babies with the power bloom mix!

I still have hope that they will all grow the weather/wind is making things difficult. The new Grow room is being framed out that will give me a more insulated space. nectar of the gods is on the right the leaf's look much bigger.

Looks like the plant is reaching its final weeks and has been doing great in my garden and is looking fantastic from now on pH water and calmag. looks Like it is within 1% of Amber so still a few days to go normally I will cut out all the fan leaf and a few Sugar Leaf then take down the entire plant and hanging upside down but because the place where I dry is the same tent but this time around I'm probably going to need to create a dry box cut them down into more manageable size because the other girls in my tent well take more than a few weeks I also can see how much of a difference does it make between hang them upside down and drying them like this thank you for reading I will continue to update have a happy grow

Everything is still fine, the oldest moved to the largest pot of 11 liters or 3 gallons and the one that grew more than the others in the pot of 2 gallons or 7 liters approx, they began to look for pots for the others later. apical pruning will be done to several practicing, as well as LST or something like that experimenting haha

Переехали в теплицу. Температура днём +27, ночью + 12.

A friend gave me a clone of his Black Domina. First week i added the recommended dose for a clone of the nectar line. I got a little nute burn so i flushed it a little with ph to 6,3 water. Looking a little better. We will see what happens in the next few days. Pictures are about a day and a half after i gave it the recommended nute dose for clones.

Colombia jack is still stretching and drank a lot of water this week. Noticed some variegation started up top so i dimed the light from 100 to 90 percent. I also added a little more far red to see if i can get it to stretch a little more. the vivo sun tent a little warmer than the other tent this week, but temps should be dropping and a little less intense going forward. I wanted to see where the plants would stretch to before seeing if i needed to make any adjustment. just a little too powerful up top at full blast. Got my temps dialed a little cooler while having lights going full blast because the plants seem to be handling it well. They stretch themselves right into the canopy where is reading over 1400 PPFD. I'm running the light so intense the lower buds at the top of the first net are growing almost the same rate as one closer to the top of second net. The plants have been drinking a ton. I watered at least 3 times about 7 gallons each time. I wanted to give them some compost tea last week, but they were drinking so quickly, I didn't have a day in between to let my water air/bubble to get it prepared for my brew. I was able to get and extra day in between and a nice dry back followed by a top dress of rock dust watered in with my compost tea with a little bit of everything I got. Each plant received top dress each 1 cup azomite,1/2 cup glacial and basalt, and 1/4 cup Langbeinite. Plants got another trim everything below the first net beforehand. I took out a few leaves between the two nets but for the most part left the plants untouched except for the a few of the oldest leaves.

Empezamos la recta final, ya corte las primeras dos, exceso de calor y agua dura (sin controlar ph) generaron defectos en las puntas de las 🍁.

After defoliating both Barbarian (=AK-47 x Barbara Bud) plants after week 11 they now develop more and amore flower clusters, since the light can penetrate deeper int the canopy now. The plants are producing trichomes like crazy, even the bigger sugar leaves are covered in them! VERY FROSTY PLANTS! The flower clusters clearly form colas now along all side branches and both plants fill up nicely. The smell is now strong and sweet with a clear peach aroma to it, it makes me smile every time I look into the tent. The plants love the Advanced Nutrients feeding schedule and are getting the recommended dose every watering.

So amazing strain

72 grams of dried and semi-cured creamy buds. smooth smoke already despite the need to drop humidity below 59% fully. not much larf surprisingly but will be fun to see how much hash each strain gets considering others in the tent had way more. Harvest: somewhat early, but solid nugs and yield - easy trimming and quick to dry compared to other leafy varieties terps: creamy dough mostly with hint of sweet cinnamon sugar vanilla - just like the pack says, but still not impressed really Drama about these seeds: got store credit on the pack of 3 seeds just because of website change to outdoor only after purchase date. excited to grow other HSC strains next year.

- Day 1 week 2 flow: watered them with 1,5 L ph 6,4 to 6,5 following biobizz schedule. Growing big and tall right now. - Day 2 week 2 flow: just checking factors and controlling humidity and air flow - Day 3 week 2 flow: not much to do, checking the tent to watch them grow:) - Day 4 week 2 flow: watered them with 1,5 L ph 6,4 to 6,5 following biobizz schedule. Defoliation has been made and humidity is the biggest challenge. Small tent and difficult air flow. - Day 5 week 2 flow: had to adjust light height and change it to full power. The limits of my setup but it’s ok not planing to do something too big :) - Day 6 week 2 flow: beautiful girls, hairs are coming ou - Day 7 week 2 flow: watered them with 1,5 L ph 6,4 to 6,5 following biobizz schedule. They have grown a lot this week, love how they can just almost double their size in flow.

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Day 36 (Bettis). Just starting to flower. Doing some minor defoliation and supercropping. Very satisfied with her progress. Day 41. Continuing with minor defoliation and supercropping.

Welcome to week 13🤠 This week I have been doing a bit of LST every day, so I've been bussy. I have also trimmed her quite a bit She's stretching a lot, almost every day I have to tie her down. She also started showing more pistils, so I'm getting excited Watered her with ph7.19 water, run-off water was at ph5.79, So I still have to raise the pH, best before full-flowering Weather was worm this week and very sunny, but it should cool down again She's not that symmetrical, but still looks nice, I'll try on making her more good looking I've also got all the material for my drying box, so hopefully I'll make it soon And I'll be making a small roof so my harvest doesn't get ruined So I think this is all to be said, I'll edit this week if I forgot to say something or made some mistakes Otherewise see you soon!🤠🤠

Für einen Steckling macht Sie sich richtig gut. Man merkt gar nichts mehr davon. Sie passt voll ins Bild.

Wow from start to finish was a great strain to grow the buds were covered in the most frost ...from the get go these beans were fast to germinate and grow with no issues when it came to high humidity or light intensity . The team at fast buds does an amazing job with there genetics .The plants were well taken care of and as a result she gave me super dense nuggets that are musky and sticky so dam sticky ..I would recommend this strain to any one who is interested in getting there first auto grow going or even a pro grower the company won't disappoint ...