Day 1 Week 10. Added AN Big Bud to the feeding as per Advanced Nutrients feeding calculator. Still fighting to keep the stretch under control. And the RH is hitting 80% leaving the door partially unzipped. Ordered a small Dehumidifier to help. Changed the cover picture in celebration of an election. Day 3 week 10. Added a dehumidifier to help bring the RH inline. Sofia and Claudia are starting to show their flowers. Happy girls make happy grower. Day 5 week 10. Each morning a defoliation, and the next day grown in again. The girls have grown enough to see where they are joined from their single seed.

This girl is loving life right now since her burn. Her root system has flourished since she was transplanted in her solo which she has left on 4.15

Mulberry Day 42 All is well. This little girl has been in stretch and now stands 38 inches tall.up until now she has been a pretty standard grow with no major issues. Using coco nutrients in soil so dosing has been a challenge. Now that we’ve come out of stretch I will start a pk13/14 regiment as I have found with autoflowers that is when they start looking for those extra flowering nutrients. Only growing the one plant anticipating the size was a good idea. *** Day 44 *** Trichomes!!! Covering everything!!! ***Day 46 *** All is NOT well today!!! Some leaves are displaying some sort of strange texture and color. Need to figure it out fast!!! Will do some research and maybe ask grow diaries a question. So thinking it’s a deficiency of some sort the plan is to water with extra calmag and a dose of nutrients with added pk13/14. Fingers crossed! ***Day 47 *** Better. She responded overnight positively to the watering and related issues. Will repeat at the next watering. 5 ml calmag. 1 tbsp coco A&B. 1 tbsp pk13/14 Ph 6.2

Those kind lights make all the difference... Especially when Power is included in rent. Lol. Not quite my pound goal, but still from just one plant. Can't complain. Gonna lose some weight during cure, I'm sure. Can't wait to smoke.

31/8/21 Day 29 #1 Ph 5.7 E.C 1.0 #2 Ph 5.7 E.C 1.0 #3 Ph 5.7 E.C 1.0 3/9/21 Day 32 #1 Ph 5.7 E.C 0.9 #2 Ph 5.7 E.C 1.0 #3 Ph 5.7 E.C 1.0 5/9/21 Day Day 34 #1 Ph 5.7 E.C 1.2 #2 Ph 5.7 E.C 1.0 #3 Ph 5.7 E.C 1.0 6/9/21 Day 35 #1 Ph 5.7 E.C 0.9 #2 Ph 5.7 E.C 1.0 #3 Ph 5.7 E.C 1.0

End of the 3rd week for the Purple Lemonade. I did some LST on her and she just got fed Goldleaf feterlizer. It's been a slow process with ups and downs. I encounter a few issues, but she's still hanging in there. Hopefully week 4 is better. I still got faith in her.

5ª settimana di fioritura per la nostra bella BISCOTTI 🍪 La benedizione di ZAMNI🗿💙 assieme ai vostri complimenti, stanno facendo diventare i bud delle pallone da golf⛳ Spero gradiate i video 😘 Grazie a tutti di 💚

The cookies have officially went into flowering and started a little bit and four but I documented it this week for sure. I’m trying to let everything catch up before I end up lollipop the bottom of my plant but so far so good. It’s looking very healthy. I did incorporate, bud candy into my nutrition, but everything‘s going well.

Läuft.........

Growing great. been adding calmag to the mix and she bounced back. Still a little yellow but the buds are growing nicely. Great trichome production To the touch it smells SO much like blueberries Im blown away and theres still more maturing to do! Excited to see how much more she fills out.

Sometimes it may be good, sometimes it may be shit. When you don't care for your plants it do be shit. Let this be a lesson for the future and also for those who think growing its just placing a seed in the ground, if you don't care for it, you get weed but not the highest grade. Altho i must say all of the auto's from this round were pretty good at smoking with the exception of one that tasted like a fart in a jar lost in the ocean for a decade, that meanwhile was eaten and shat by a whale, not good, but the others were pretty good, enjoyable to say the least.

On day 56 the girls were 70/30 cloudy to amber trichomes (my preference) they were ready for harvest. I took clones from pheno 3 and pheno 5. I will be monster cropping these clones to preserve their genetics. the girls went into 48 hours of darkness right after. On day 58 the girls were chopped. The tent was cleaned top to bottom and we started our drying process. We will be shooting for 60-65 percent RH with a temp of 60 degrees. I expect this dry to go 10-14 days. I will update a final dry weight, pics, and smoke test in a few weeks after the cure. I’ll also be posting my last seed to harvest episode of this series on my YouTube channel that can be found in my profile link or “probation2propagation” on YouTube. Stay tuned.

Already showing some flower buds. It's developing well. Did some hard defoliation. smells good

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.