Este es el avance finalizando la 3ra semana, tienen 21 días, ahora les haré una defoliación y una poda de bajos, van maravillosas las Gorilla Banana de BSF Seeds. Planeo pasarlas a floración el día 31 para cerrar con 30 días de vegetación. Saludos @farmer.skr 🤙

All content on this diary is for inspirational and educational purposes only. The ideas shared are not a substitute for professional advice. This diary/account is not officially affiliated with Alan Watts or his estate. All materials are used under the principles of fair use. I honor the legacy of Alan Watts by sharing his wisdom respectfully and with the intention of inspiring awareness and self-understanding. 2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

3 erma cutted

Starting week 5 of flower. Plants are starting to bulk up! Nanaz x BBC smells fruitty and gas, Headband #2 smell citrusy and Headband #1 smell les citrusy and more like grass

Hello This is the end of week 10 and the beginning of week 11 of veg. Going good in here. Got some trimming done. Got the bottom trimmed up and sucker branches taken off. I've taken off enough leaves that you can barely see through the plant now. Don't take off too much. It will take a few days for the plants to get over the trim up and heal up, and then I'll start to flower next week. OK. Be Cool. Chuck.

Starting to fill out nicely now

this wedding cheesecake ff is the healthiest plant i have grown with massive fan leaves these two girls have been in bloom for 1 week can’t wait for the buds to start forming

Flushing begins for Do-si-dos. Her buds are swollen AF and she's got a strong fuel, industrial smell. Not much else to say about this girl. Thanks for checking out my grow 👽🌳

June 26 Getting bigger and bigger! Makes me wonder if I should flip her to flower here soon to control the size since she's not an autoflower which is usually smaller, genetically. She hasn't used up all of the nutrients in the bucket yet either. June 27 Plant was a bit droopy, so a half gallon of PH'd water did the trick. June 30 Some lower leaves are getting yellow and dying. I'm taking that as a sign that the soil is almost out of nutrients. Mixed up a 1/8th strength of General Organics GO Box nutrients (week 1 to avoid nute burn), but upped the CalMag to 1/4 strength. Fed one gallon at a PH of 6.7 to slight runoff.

heat alarm: 2 days outside temp is 40˚C, in tent 31°C / 65 rh (is in acceptable vpd-range -> no change) signs of heat stress (1 ww+bb/1 bg) and few mould on surface of soil running tent open with add. osc. fan very few nute burn every 2 days basic water; weekly basic water + nutes 2 more bgs heat stress ____________________________________________ light- and watering schedule: see photos now: 16 plants 3 x Auto Orange Bud > Dutch Passion (early80's) 3 x Haze Berry Automatic > Royal Queen Seeds (2018) 2 x Auto Euforia > Dutch Passion (late 90's) 2 x Auto Bubblegum > TH Seeds (late 80's) 2 x Original Auto BubbleGum > Fast Buds Company (late 80's) 1 x Strawberry Pie Auto > Fast Buds Company 1 x Gorilla Cookies Auto Seed Stockers (2015) 2 x Auto White Widow x Big Bud > Female Seeds setup + strategy: 18 seeds 3 x Auto Orange Bud > Dutch Passion (early80's) 3 x Haze Berry Automatic > Royal Queen Seeds (2018) 3 x Auto Euforia > Dutch Passion (late 90's) 2 x Auto Bubblegum > TH Seeds (late 80's) 2 x Original Auto BubbleGum > Fast Buds Company (late 80's) 2 x Strawberry Pie Auto > Fast Buds Company 1 x Gorilla Cookies Auto Seed Stockers (2015) 2 x Auto White Widow x Big Bud > Female Seeds setup: 18 x gronest 2 liter 60 cm x 120 cm x 180 cm (2 x 4) growtent 4 l humidifier 25 watt axial fan 15 watt clip fan ro-filter bath room with a 50 watts room fan (-> carbon filter not necessary) ...and a lot of odds and ends. grow strategy: max yield by stressing with: 1. tiny shoes (2 l fabric pots standing on 3 plastic rings (4 cm) for max oxygen) 2. tiny growspace (18plants on 0.72 sqm) 3. many strains (8 strains) 4. annoying neighbourhood (mixing old - i.e. bubblegum (late 80's) - with young- i.e. hazeberry (2018) - genetics) 5. no stress by light or food or water 6. unintentionally too much food plus: - no lst - no hst - just leaf tucking - positioning bigger strains (euphoria, orange bud, haze berry) or just bigger phenos on less intense light spots (end of tent + edges) - music-rotation: 24 hrs reggae (for sativas) - 24 hrs classicals (because it's scientifically proofed) - 24 hrs traditionals from the hindukush region (for the indicas) max efficiency (min electricity - max yield): - light: 23 h on - 1 h off - keeping the distance of 18" (45 cm) and dimming to the right par (lux) - value depending on growing stages (see sheet: beginning 185 par ( 10000 lux/100 watt )/end 340 par ( 18000 lux/175 watt )) => ends up in approx. 170 watts in average over max 15 weeks temperatur management: - using approx. max. half of what the lamp can do keeps temperature low: my tsl 2000 is pulling max. 360 watts of the wall - i need only 175 watts, results in less temperature than using a 175 watts lamp - adjusting the temperature by using a humidifier outside of tent: blown in humidity is soaking degrees and is transported out by fan, works much better than doing the same with pure air summer extreme: no humidifier: 36°C / 35 rh - with humidifier: 29°C / 65 rh works for 1-2 weeks of real hot summer days, no mould, no signs of stress in two summers germination: seeding in waterglass spraying "basic"-water: ro-water (22 ppm) + calmag to 180 ppm every 4 - 8 hours until sprouted man versus fungus gnat: 5 days before seeding i'm running the tent with "basic"-watered pots as if there were plants inside - led on, fans on, humidifier on. Fungus gnats coming with the soil might take the chance to come out now for breeding. i'm waiting with a 9 mm rifle. in the past i found 6 gnats in 2 of 8 x 50 l biobizz lightmix bags coming out of the soil. i could eliminate every single gnat successfully ;) rotating harvest: the little ones will be harvested completely when ready. at this point only the top branches of the big ones will be cut...and the new (basic-watered) pots of the new grow will move into the tent. 2-3 weeks later the secod half of the big ones will be cut and the second half of the new grow will move in... nutes: biobizz / soil: biobizz lightmix bloom,grow,topmax,activera,algamic + aminopower instead of bio heaven (too expensive) individual feeding schedule/once a week: veg: 200 - 600 ppm bloom: 600 - 900 ppm water: ro-water with: veg: 100-150 ppm calmag bloom: 150 ppm calmag

So close yet so far

Hello everyone week 2 has passed for this LSD-25 auto by Fast Buds 😎 this plant is doing very well growing at fast pace and with a beautiful green colour on the leaves. Topping & LST for my girl 😎 wish you all happy growing!

Lower leaves died and so I needed to defoliate plants grew quite a bit in this stage and preflowering shows they are girls temp dropped outside finally and so dehumidifier is constantly running. With a nice 78 degrees 50 rh

Purified water and 1/16 of a scoop of great white mix well. 3 seedsman zkittles auto seeds in a piece of reverse rolled up 210 grit sand paper. Shook the beejezus out of it till my arm got tired to scour the shell a bit. Tossed it in the jar of tea. 24 hrs later she popped. I replanted in rapid rooter plugs on oct 6, and with in 1 day 1 plug popped, The next day the other and on the 11th I noticed the 3rds cotyledons we’re stuck in the plug. I helped her a little and by the 12 all 3 were ready to be planted in their final home, A 3 gal fabric pot filled with my mix of 1 bag ff ocean forest : 1 bag of ff light warrior. Each lady was 2” high with the first set of true leaves and nice little roots peaking through. I’m having an issue with my purified water taking 24 hrs sitting before I get a stable ph so when I measured the res water in my dome, what I thought was 5.5 now showed 8.0 😐. I fixed that and watered all 3 with the correct ph water.

A wonderful genetic thank you Fast Buds ❤️‍🔥🤩 The trim was super easy the buds are FAT & FROSTY full of resin 🤤 I’m very happy with the harvest 96g !! For the light i’ve used the Spider Farmer SE-7000💡