Last week!! Flash Clean and water only

November 6, 2021 They are both still going. The Blackberry is starting to foxtail. I still don't see any amber trichomes and I have not seen the calyx's swell yet. These plants have 2 more weeks max. As soon as the photos are ready to flip to flower, I'm harvesting these autos - ready or not.

So far she looks like she's doing well. I'm not getting any leaf curling from either over watering or nutes so I'm happy with that. Just some minor leaf discoloration. I feel like she exhausted her nitrogen early.

Week 3 is done and this girl had an interesting week. I noticed her yellowing tips on day 17 but only on the older growth? I'm stumped, surely it isn't the light intensity, still only at 450ish umols. The pot is always weighty, could it be an over water? Her sisters don't seem phased at all. As for environment, today I've turned the ventilation fan on 24hrs with no breaks. I started getting paranoid that hour on hour off is not doing them any favors and I'm just gonna have to suck it up and bare the brunt of the power bill. This will also drastically reduce the RH, which I'm not totally sure of the effects it will have on the girls. Low RH has got to be better than RH too high, right? 🤷‍♂️

Hello growers This week was a good week. The plants are still growing good. Good color. Happy. In a week i will switch! See you

05/01/2021 Better pics coming in the morning, bringing her outside for natural light see how she looks. I never realized how frosty she is, and still believe her to be the lemony lime pheno, which will be great for my outside grow! Two clones taken one for outside one to keep as a mom.

Plant is fine, except one stormy night the weather is just fine. Heard that is gonna be a nice September, hope they are right. I plan to cut the tree on the second/third week of September. Yes some yellowing leaves, I still think is the aging process and a little bit of K deficiency, that should have decreased, and looks like it has, new growth are just fine and there is no worsening. Still watering with: Big bud, 2 ml x L CalMag 2 ml x L Bio Bloom 3 ml x L silica, one tablespoon neem oil propolis aloe

@ 1.2-1.3 kPa

Germination and Preparation The Do-Si-Dos seeds from WeedseedExpress arrived well-packaged in a discreet envelope with bubble wrap and a branded shopping bag. The seeds look promising—large, with beautiful marbling. I started by soaking the seeds in water with a pH of 5.8. After just a few hours, one seed already sank to the bottom, not a good initial sign. Despite this, I kept all three seeds in the germination process, and all of them developed roots. The seed that initially sank seems to be doing fine, so I’m giving it a fair chance. The seeds are now placed in Jiffy pellets with a pH of 6.0. The temperature is maintained at around 24°C (75°F). My plan is to monitor these seedlings closely and select the most vigorous one for cultivation. Grow Plan I will be using the Sea of Green (SoG) technique in an 80x80 cm space. To maximize the yield and structure, I intend to extend the vegetative stage to about 6 to 8 weeks, possibly up to 10 weeks. I’m also using organic nutrients, and the motto "Don't Panic, It's Organic" fits my grow style perfectly. Dosidos End of Seedling Phase Report Environmental Conditions: Humidity: 70-80% initially, adjusted to 60-70% during hardening. Temperature: 19°C (night), 23°C (day). Lighting: Lamp positioned 40 cm above seedlings. Observations & Actions: All three seeds germinated successfully. One seedling was removed for poor growth and exposed root. Greenhouse lid removed on day 7 for airflow; seedlings adapted well. By day 9, roots fully developed, signaling readiness for transplant. Transplantation: Transferred to 7L pots with light watering and BioBizz Root Juice to ease stress. Performance Feedback: The two selected seedlings show robust growth, forming a solid foundation for the planned Sea of Green setup. dein-onlineheadshop.de/ Use my referral code 420Shop at checkout to get a discount on your purchase. By using this code, you also support me, as I receive a small reward for each use. Thank you for your support!

Hello everyone , Farmers friends I had to bend my sweet little girl because she approached too much to light .... the result? An explosion of ever seen colors !!! This is my first red genetics and it's great to see these gems color himself

Lilly x White Widow is growing great. I did a solution change on her 4 days ago. She is bulking now, and starting to give off a aroma. She has a nice layer of frost started as well. Thank you Spider Farmer, Athena, and Ripper Seeds. 🤜🏻🤛🏻🌱🌱🌱 Thank you grow diaries community for the 👇likes👇, follows, comments, and subscriptions on my YouTube channel👇. ❄️🌱🍻 Happy Growing 🌱🌱🌱 https://youtube.com/channel/UCAhN7yRzWLpcaRHhMIQ7X4g Spider Farmer Official Website Links: US&Worldwide: https://www.spider-farmer.com CA: https://spiderfarmer.ca UK: https://spiderfarmer.co.uk EU: https://spiderfarmer.eu AU: https://spiderfarmer.com.au G5000 Light Amazon Link: amzn.to/4643esa UVR 40: https://www.amazon.com/dp/B0BR7SGTHS Discount code: saveurcash (Stackable)

I have a very small bit of powdery mildew on the girls. I have been using CuSo4-copper sulphate about every 3 days to keep it at bay. It seems to be working really well. They look great, are happy and are working hard on plumping up their flowers. I have 4-6 weeks until harvest would be my guess. Fingers crossed I can keep the effing PM from taking over. 😬

Now our girls are looking a little bit better. A few of the plant louses survived the treatment with the garlic brew. So we choose another average to beat them. We made a mix of Kali soap (Sapo kalinus) with water and grain alcohol. We use it yesterday in the morning, also today and we really hope it works now 💪

After 72h darkness time to cut this ladies. Smell is stronger that I imagine. Now 7/10 days drying, aiming for a slow dry and then a proper cure.

On day 70 they both went down. The trichomes showed the were ready. The last week I kept the girls in the shade a bit during the day. We´ve had temperatures close to 40 degrees C. I didn´t want to expose them to too much heat.

This week marks the end of Week 10, and Do-Si-Dos Auto is deep into her final ripening phase. Trichomes have shifted to mostly cloudy with a few ambers starting to show, signaling the early harvest window. Her fade is well underway with noticeable yellowing and some purpling on the fan leaves and bracts — all expected at this stage. She experienced one dryback early in the week that caused temporary leaf droop, but she perked back up quickly after a good watering. Temperatures peaked at 86°F with humidity holding around 45%, keeping the environment within range. Buds are dense, frosty, and continuing to swell. On the final night of Week 10, she was watered with Cal-Mag (1 ml/gal) and the auto-watering base was refilled. Harvest is likely coming within the next week depending on trichome development.

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. 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Day 64: unquestionably in flower. Added one gallon of pH 6.5 water with nutrients. Day 65: No picture. Day 66: yikes! Left her in the dark for 24 hours. Forgot to turn the light back on after day 64's Glamour shots. On the opposite side, the damaged branch is upright. Perhaps it won't be lost. Day 67: looking thirsty. Added one gallon of water with early flower nutrients. One leaf has holes in it (not pictured). Will have to watch for pests. Day 68: Man this plant is thirsty. I don't want root rot so I will wait one more day for watering. One leaf has odd holes in it. It is directly in the path of the fan so I wonder if it is related to that. Day 69: added one gallon of ph6.0 water no nutrients Day 70: Nothing remarkable to report