She is exploding!! Unfortunately can’t be at home as I’m away from work to take care of her. She needs some training but I don’t think I’m going to get home in time to do it.. may have to just let it do it’s thing. I’m glad I topped it though. I’m a little worried I’m going to run out of vertical space.

6 Blütewoche: In der 6 Blütewoche habe ich bei zwei meiner Ladys Cal-Mag Mangelerscheinungen festgestellt, die ich mit jeweils 1.25 ml cal-mag Lösung in 1l Wasser gegeben habe. Ansonsten wachsen und gedeihen die hübschen, die Buds fangen an frostig zu werden, ich hoffe das in den letzten 2 Wochen der Blüte die Buds noch an Größe zunehmen. Ansonsten bin ich sehr gespannt wie sich die Ladys noch entwickeln werden. Ich werde euch auf den laufenden halten, viel Dank an euer Interesse. Bis next Week

Day36, 37, 38: Seems to be growing a set of leaves per day now. Super growth! Stretching to the top. Day39: I had a hiccup with the camera. For some reason it stopped responding at 04:30 in the morning. I only realized it much later. I still don't really know why, but reseating the data ribbon fixed the issue. Day40: After fixing the camera, I decided to take this view again. The Lady is starting to show some nice growth and this view will show it best. If you look carefully, you'll see that her branches are not green, but rather reddish. Definitely related to genetics :) Everything else is within limits. Plus, the leaves look super healthy. Day41: Here you can see the stem colour better.

The second plant did not respond nearly as well to some drought stress tests. We use it for comparison. Consider this an experiment in stunted growth due to damage in early veg.

So this week I added nutrients for the flowering stage. I gave her a little trim as well as added her netting. I flipped her on last Sunday PP

This little girl is comming in good on begining her 2 week of veg she's growing awesome under this fold 6 from medic grow an she's growing good using this cultured biologix nutrients Purple Goat Cheese from Goat Genetics is doing great too growing noticeably more everyday under this medic grow led

En esta semana se bajaron los niveles de ph y ppm. De 6.5 de ph bajamos a 6.2,6.3 Y las ppm de 700 la llevamos a 1080. Se aumento la dosis de las bases de 2 ml/L a 3ml/L. Se realizaron todos estos cambios para mejorar la calidad de las hojas, las puntas están con un color amarillo, y no se si es algún exceso o carencia a causa de la calidad del agua. Pero en términos generales esta muy bien la primer semana de floracion,quedan muchas semanas más por delante.

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Y diganme que problema tienen con la yerba del Rey no me dejan fumar y me persiguen con su "ley" El Rasta solo quiere fumarse un "may" A su-play, everyday Castiguenme si hago daño a alguien y si no dejenme se lo digo claro no lo repetire El Ganja esta bendito por el Dios de Moises persigan asesinos y no creyentes. Read more: Morodo - La Yerba Del Rey Lyrics | MetroLyrics

Beginning of week 2(Bloom): This girl has exploded! She is getting huge fast. I’m really happy with the progress of this girl. Pictures this week are taken 1 week after 12/12 light started. A few of my leaves were looking somewhat waterlogged, that’s the best way I can describe it. I’m thinking I’m over watering her, but then again the growth has been great. I’m going to cut the water to half of what I’ve been giving and see how she looks in a few days. This girl looks like she will be quite a big bigger than my last grow. I did some defoliating to her last night. I’m trying to stay on top of the new growth under the canopy. I’m not sure how many times I should be removing the growth. But If I notice something decent sized under the canopy that will just leech I’ll clip it. Hope it’ s not too hard on her. Thanks for checking out my grow!😊

Update Day 14 Flowering: Pog is ambling along stacking her neat little buds up ready for long colas i hope. Temps here have been crazy hot for the UK. 34°c at points which is slowing them a little but with lots of airflow incoming , the rh is not too bad at 70% and i am watching for mould like a sheepdog for wolves.lol Another happy Barneys Farm Girl

Day 22 Update: LST rechecked and adjusted as necessary. All three got dechlorinated tap water, PPA1 and PPA2 each got nearly a L, and PPA3 got about 1/2-2/3rds. Day 24 Update: LST rechecked and adjusted as necessary. All three got dechlorinated tap water; PPA1 and PPA2 each got nearly a L, while PPA3 received about 2/3rds. Pictures show decent foliage filling in over the past two days, so I adjusted again. One, maybe two more good adjustments and then I'll mostly let em grow. PPA3 seems to be recovering from the topping well, but I wouldn't know the difference yet anyways. Its fun to watch them grow regardless! Day 25 Update: Just took a picture of each today. Day 26 Update: Took a picture of each in the tent. They will likely all be getting water tonight. I think just regular water again tonight. The tip with 2 sets of stems broke off PPA2 so they’ve all been topped if inadvertently. I always had my doubts because of the soil PPA2 is in, but this will drastically affect the outcome. Day 27 Update: I watered them with about a .75 L each tonight. Decholorinated tap. Maybe add some Tiger Bloom next time? Pretty sure the soil was still pretty hot at least with nitrogen from whatever I had growing before, so maybe just water again honestly. Updated pictures of each today. PPA2 is going to be severely limited. I looked through the pictures, you can see the break on PPA2 on day 24. I probably could have saved if I'd noticed, so I've learned to keep a better eye on the training and not force the main stalk so much. I don't know if I'll adjust the training anymore. Day 28 Update: Just a new picture of each. There’s also a video showing off my whole tent. Light dose of Tiger Bloom tomorrow night is the plan.

Start of week 5, the girls are looking promising. Trichome production is in full flow now, leaves and buds getting very frosty. Slowly increasing the EC in 0.1 increments to avoid overfeeding and nutrient burn but still push the genetics. Also added a dehumidifier to help keep the humidity down and in range. Although not a whole lot to do, pH and EC parameters are still very important. Will be checking in, the next few days.

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Я так и не понял FastBuds, может потому что в моей стране война, каждый день были ракетные обстрелы и я не смог нормально выращивать. Обновления 13.05.2023 русские террористы продолжают убивать мирных людей. Очередной ракетный обстрел Украины 🇺🇦🇺🇦🇺🇦. Моё утро началось не с кофе а из 13 взрывов ракет. россия и русские это террористы, убийцы, преступники.

When should they start flowering?

Opium week 16 7/22-28 This week has been rainy so the outside girls only got watered and fed once. with all the rain we've had, all the girls grew a bit. I top dressed all girls with Bloomin Soil from Elm Dirt. Photo Opium is now 75 inches and stacking nicely. Happy girl means happy mom.