Growth has been steadier, and I’m particularly pleased with the new watering system. It didn’t take long at all for the root system to attach to the ropes

ordered 10 gallon pots for the 2 blue dream and pineapple express they are going into a different tent. Plan on replanting Friday. Autos all are doing fine, think I've been having issues with cal mag hopefully I can get it sorted out. All the plants are pretty big at this point except the gelato #33 its so small lol. Very crowed in a 4x4 and the plants need more room maybe moving the photos out will help the autos out and let them spread out more. Ordered some Recharge, excited to try out. I heard about it on dude grows lol great podcast 👏 Picked up some blue planet liquid seaweed and also their gold shield silica, also excited to try. Lastly got some Dynomyco to add in. Both blue dream and pineapple express are in their new home another 4x4 tent. Only running 1 halide at 400 watts and a small mars led. Will boost the light up significantly during flower with 1200 actual watts and change out to super lumen hps. Running a terrabloom carbon scrubber out of the second hid hood all powered by a 6 inch AC Infinity cloudline venting outdoors. Also using another 6 inch to bring in fresh air and have a portable ac to keep it nice and cool I think its important to mention after every grow I like to go in and clean out my tent. Vacuum up leaves/dirt, Wipe down all surfaces of the tent(I use paper towels and water in a spray bottle) and also clean off my lights. Wipe them down make sure the glass is cleaned(It makes a big difference). I grow strictly for my own personal consumption and I want the cleanest product.

Week 6 2nd week of flowering: Slowly but surely, the whole thing had taken shape. And in the way I'm usually used to. "Sow love, reap happiness." Hello dear plant friends, please forgive me for my time delay in the grow diary. A lot is happening in real life for me right now and I had to get used to it. Unfortunately, one of my beloved cats died suddenly for a reason unknown to me. A long-time friend and companion. And in addition, I am very busy modernizing my grow room, expanding capacities and the current grow also requires a lot of attention, as I am passionate about new crazy training methods. All this distracts me from my grief and the loss of my dear furry friend and helps me a lot to get back on my feet mentally. Well, I don't want to keep you in suspense any longer, so here's a little preview of what will change and why.... 🤩 But that was only a short fitting and both lamps will of course come in a new generous Homebox 100 Q + I think the time has now come for me to leave the tried and tested old shell and go new exciting ways. I feel ready for the next challenges in this wonderful field of gardening. Dear people, be curious about what is to come. I can't wait to share it all with you. And now back to week 6 and last summer's Grow... Enjoy! 😊 And again my Biotabs was my secret weapon: the fermented compost tea an outstanding game maker. I usually start at the end of flowering week 2 and combine it alternately with the enchanting Bio PK - Booster from Biotabs until shortly before the end. I am firmly convinced that these two components complement each other super nicely.

So Friday will be day 70. I messed up a lot, did lst and used big pots so I'm thinking these might go 3-5 more weeks. They smelled so amazing a few times today though. First the purple punch, then the sour diesel, then the girl scout cookies, each gave off aromas one after the other a few times today. The purple punch smalls so sweet and delicious.

Just as difficult to grow as the first time I done Afghan kush but still managed to get some nice purple bud out of it!!!

Die Seni Seed ist übelst abgegangen hat sehr schöne Ergebnisse geliefert, es haben sich zahlreiche dicke Blüten entwickelt😜 Bin gespannt wie es noch weiter geht mit ihr! @SensiSeeds Mega Sorte 🤗👌

Started lsting both purple punches

Time lapse is from 7th to 12th May. Environment it good so far. No issues...all looking good! I'll pot these up end of the week when they're in the main tent. Hopefully see some rapid growth once in there! Peace.

05/18/22 changed nutrient water PH 6.20 TDS 870 now on bloom nutrients. Will give foliar feeding tonight at lights out. 05/19/22 PH 6.13 TDS 890 gave LST to even canopy and get some airflow. 05/20/22 PH 6.19 TDS 878 05/21/22 PH 5.95 TDS 906 05/22/22 PH 6.15 TDS 750 05/23/22 PH 5.95 TDS 780 05/24/22 PH 5.90 TDS 850

Day 21 I defoliated all the purple stem fan leaves. Now feeding full strength additives. I will leave them in two litre pots too finish up.

Cinderella became a big bud . Already giving her plain water for a few days and on Saturday is harvest day. Last defoliation of sugar leaves for o allow more airflow

800ppfd

Die feminisierten stehen kurz vor Beginn der Blüte und wachsen immer langsamer in die breite. Critical Auto ist fast fertig. Noch ein paar Tage (gutes Wetter ist angesagt) und dann wird auch sie geerntet. Creamatic ist geerntet und trocken mit 23g besser im ertrag als erwartet. Es läuft gut 👍 Bis nächste Woche ✌️

They got moved into the 4x4 with the jimmy rigged 180w quantum board light built from ceiling tile grid. The tnb co2 is newish but I gotta get the heat up and bring in a more powerful light to take full advantage.

16.2.25: I decided to check out the lower canopies of all plants to see if I need to get rid of any foliage. I did get rid of a few small branches and leaves. However, whilst I was doing this, I saw more garden pegs from my LST remaining. There were about 6 or more in Pink Mist alone. Additionally, on Watermelon, there were some left in, too. I'm so annoyed to see that because the plants are really stretching, and I could've potentially disrupted this by leaving the pegs in for all this time without realising it. 🤞 that I haven't compromised things too much. We'll see. I watered today with 2ltrs of dechlorinated water PH'd to 6.3 containing the following nutrients; ♡ .8g Green Leaf Nutrients PK booster ♡ .5g Ecothrive Biosys I ordered quite a few things for the garden. I got Greenleaf Nutrients Sea K(elp) and Mega Crop Parts A+B. To go with their PK Booster I got last month. I'm excited to try it all together. Next run, maybe just using these. We'll see how it goes. 18.2.25: The plants are going crazy for water! Everything is getting used right up so fast! Today, I decided to add some more Black Strap Molasses to add some carbs and other micronutrients. I'll add the jar with the label in the photos section above. I watered a very small amount to each plant. What I put in: ♡ Black strap molasses 150g ♡ 2g Sea K(elp) Greenleaf nutrients. I dissolved everything in 4ltrs of dechlorinated water PH'd to 6.4. 19.2.25: I received the majority of the garden purchases that I made. I'm still waiting for the Ecothrive Life Cycle. I wanted to top dress, but it's been delayed unfortunately. I am using my Greenleaf nutrients products which I bought on Amazon. I got the Mega Crop 2 part system Part A and Part B. I have the Sea K(elp), and the bud explosion PK booster. I really wanted to get some of their sweet candy asking read many positive reviews. Unfortunately, for me, this is unavailable to buy currently. So that's a little disappointing. I needed to do a good watering so when my nutes were delivered today, I got excited 🤗 I watered 2ltrs of dechlorinated water per plant, PH'd to 6.4, containing the following nutrients: ♡ 1g Mega Crop Part A ♡ 1g Mega Crop Part B ♡ .5g Sea K(elp). The plants drank this up within a few hours. I'm going to try and hold off on watering in hopes that my Ecothrive Life Cycle will arrive so I can top dress and water it in then. 20.2.25: My Ecothrive Life Cycle arrived yesterday, and the plants are ready for their top dress and a good watering in. I have some Biobizz Light Mix, Canna Coco,and perlite. I'm going to use this as a base to mix my amendments in. I'm going to fill my 5 gallon bucket with about 4.5 gallons of my top dress mix. I will distribute this across 6, 4-5 gallon pots. Then I will water in well with Greenleaf nutrients Mega Crop Parts A+B and Sea K(elp). I've made a crude attempt to video mixing my top dress. Don't listen to the audio. lol, my YouTube didn't stop playing whilst I recorded this 😂 So anyway, I added the following amendments to the above base mix of 4.5 gallons; ♡ 3 TBSP Ecothrive Life Cycle ♡ 3 TBSP Vitalink Bat Guano ♡ 3 TBSP Ecothrive Charge ♡ 1 TBSP RHS Mycorrhizal Fungi granules ♡ 6 TBSP Ground Cinnamon.

All good apart from the summer heat, but nothing I can do about that, plus in seedling stage the young ladies can handle it really well so no issues! 👍🏾🌱💚

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.