Week 2 of Flower (Day 56 since sprouted) Just maintaining temps and adjusting light distance. Fed with 1/2 gallon of feed water PH 6.5 -TPS CalMag 1 mil -General Hydroponics Trio -TPS Signal 5 mil per 1 gallon Light defoliating

Defoliated and doing their own thing. Fattening right up I just love it!

|»» LOG DISCLAIMER: Not intended as a grow guide. Grow log entries are usually posted 3 weeks later and are for my learning reference. ««| Introduction - Week 26 Summary - Ensign's log, stardate 17608.3, Wow what a week! Drying is always pretty simple and been checking daily. Buds are drying nicely and terps are smelling pretty dank!! Most of the week has been in the veg tent with tissue culture! Mixed another batch of establishment media and took proper explant cuttings. Also super-cropped the mothers to even out the canopy. Ahh yea! Getting hyped for trim day next week! Next week will also be the last week for this grow log, but am already planning out the next cycle. Anyways! Onwards to next week! [START OF WEEK 26] ========================================================== ---------- [ 4x4 MAIN TENT ] ---------- Heady OG BX1 #1 [Round4 ~Wk19] - WEEK 26 - Dry Wk1 ----------- [ 3x3 VEG TENT ] ------------ TKO - WEEK 09 - Full Veg Gorilla Goat #1 - WEEK 05 - Full Veg Heady OG BX1 - WEEK 09 - Full Veg Garlic Budder OG#1 - WEEK 05 - Full Veg 10/13/25 → 10/19/25 This Week's Estimated Goals ---------------------------------------------------------------------------------------------- DLI: ~0 mol/m²/d PPFD: ~0 μmol/m²/s VPD: ~1.5 kPa This Week's Meter Readings & Averages ---------------------------------------------------------------------------------------------- Light On/Off: 00:00 - 00:00 | Wattage: ~000.0W [LV00] Distance From Canopy: ~00” DLI: ~0.00 mol/m²/d PPFD: ~0 μmol/m²/s VPD: ~1.17 kPa Soil %: [47.4, 34.1, 35.0, 55.7, 43.7, 43.6, 44.0, 43.3] ========================================================== Day 01 - Monday - 10/13/25 - Total Days: 183 ----------------------------------- Daily Averages ~[+01:00] - Temps: 78.8°F [26.0°C] - Humidity: 64.4% - VPD: 1.19 kPa - Soil %: [49, 34, 35, 57, 44, 44, 44, 42] ----------------------------------- - [22:05]: Night check on the 4x4 tent. --- Ca-Caw!! Looking great! --- Heady OG BX1 #1 is drying along and no signs of mold. Also airflow and RH is stable and within the 60-70% range. --- Getting stoked! Trim day is 9 days away! - [22:09]: Checked the Ecowitt soil moisture sensor data: ------------------ [ 4x4 MAIN TENT ] --------------- ------ 48% - [CH01 FT » G30 - Heady OG BX1 #1] ------ 35% - [CH02 FB » G30 - Heady OG BX1 #1] ------ 35% - [CH03 BT » G20 - TKO] ------ 56% - [CH04 BB » G20 - TKO] ------------------ [ 3x3 VEG TENT ] ---------------- ------ 46% - [CH05 BL » GR7 - TKO] ------ 40% - [CH06 BR » GR7 - Gorilla Goat #1] ------ 40% - [CH07 FL » GR7 - Heady OG BX1 #1] ------ 40% - [CH08 FR » GR7 - Garlic Budder OG#1] --------- Veg: 38% - 45% | Flower: 45% - 55% --------- General: 30% [Dry] «| 45 - 60% [Moist] |» 70% [Wet] - [22:18]: Checked the 3x3 veg tent. --- Looking healthy in the veg garden! --- All of the vegging mothers are looking great and the canopy is starting to even out. --- Also clones look healthy and no need to water yet. --- The stems, err, I mean, explants, don’t show any signs of contamination. --- Staying positive, glad the growth media is sterile and the overall procedure was successful. --- Anyways! Plan to create more establishment media and cut proper explants soon! - [22:21]: Checked the humidifiers. --- Tanks are still full and no leaks found. Day 02 - Tuesday - 10/14/25 - Total Days: 184 ----------------------------------- Daily Averages ~[+01:00] - Temps: 76.9°F [24.9°C] - Humidity: 64.8% - VPD: 1.11 kPa - Soil %: [46, 34, 35, 56, 43, 43, 44, 43] ----------------------------------- - [21:44]: Night check on the 4x4 tent. --- Looking good in the dry tent! --- Heady OG is drying along and humidity levels look great! --- Nothing to do but maintain and wait to trim in 8 days! - [21:55]: Checked the Ecowitt soil moisture sensor data: ------------------ [ 4x4 MAIN TENT ] --------------- ------ 45% - [CH01 FT » G30 - Heady OG BX1 #1] ------ 34% - [CH02 FB » G30 - Heady OG BX1 #1] ------ 35% - [CH03 BT » G20 - TKO] ------ 56% - [CH04 BB » G20 - TKO] ------------------ [ 3x3 VEG TENT ] ---------------- ------ 41% - [CH05 BL » GR7 - TKO] ------ 40% - [CH06 BR » GR7 - Gorilla Goat #1] ------ 47% - [CH07 FL » GR7 - Heady OG BX1 #1] ------ 46% - [CH08 FR » GR7 - Garlic Budder OG#1] --------- Veg: 38% - 45% | Flower: 45% - 55% --------- General: 30% [Dry] «| 45 - 60% [Moist] |» 70% [Wet] - [21:59]: Checked the 3x3 veg tent. --- Looking healthy and growth is raging! --- All mothers are looking beautiful! --- Clones are getting taller and looking healthy! --- Gorilla Goat #2 needs watering. Let’s go Ensign! - [22:03]: Here is the starting initial watering range: ------ Gorilla Goat #2 » 255.4g → 300.3g and was last watered 4 days ago. --------- General Solo Range: [Dry] «| [Initial Cup Weight] + ~55-100g |» [Wet] --- Took the starting weight before watering, ------ Gorilla Goat #2 » 230.8g --- While on a scale, --- Gently misted her straw mulch with RO water. --- Tried to keep the top growth dry. --- Misted 5-10 sprays. Waited a few seconds, then misted again. --- Made sure to reference the watering weights. --- Repeated this process until the cup weighted at least 300g. --- There was no runoff. - [22:23]: Took the saturated weight after watering, ------ Gorilla Goat #2 » 345.7g --- Marked the watering date on the side of the cup. Also marked the saturated weight on the back. --- Placed her back into the tent. --- Decided to train and super-crop both clones. --- Bent the taller branches to lower the canopy. - [22:38]: Checked the humidifiers. --- Tanks are still full and no leaks found. --- Onwards Ensign! Day 03 - Wednesday - 10/15/25 - Total Days: 185 ----------------------------------- Daily Averages ~[+01:00] - Temps: 75.6°F [24.2°C] - Humidity: 63.8% - VPD: 1.09 kPa - Soil %: [48, 34, 35, 56, 44, 43, 44, 42] ----------------------------------- - [+01:33]: Quick night check on the 4x4 tent. --- Looking great in the dry tent! --- Also starting to smell dank! --- 7 more days until the big trim! - [+01:37]: Checked the 3x3 veg tent. --- Veg garden is healthy and growth is green! --- Both the Heady OG BX1 #1 and TKO mothers will need another topping soon. --- Clones in the propagation tray also look great and no need to water. --- The explant media is still looking sterile with no signs of contamination. --- Easy night in the garden. Nice! - [+01:40]: Checked the humidifiers. --- Tanks are still full and no leaks found. --- Okay! Tomorrow plan to dive back into tissue culture and prep more growth media. --- Onwards Ensign! Day 04 - Thursday - 10/16/25 - Total Days: 186 ----------------------------------- Daily Averages ~[+01:00] - Temps: 74.6°F [23.7°C] - Humidity: 62.2% - VPD: 1.11 kPa - Soil %: [46, 34, 35, 56, 43, 44, 44, 45] ----------------------------------- - [11:41]: Alright! Back to tissue culture and time to start another batch of establishment media! --- Took the plant growth regulators out of cold-storage (my fridge). ------ [ » SMOKES A BOWL « ] --- While the products warmed up, gathered the necessary supplies and setup my workstations. --- Currently, focusing on: ------ PHASE 01 » ESTABLISHMENT --------- » DAY 01 » MEDIA - PART 01 - Mixing the growth media --------- » DAY 01 » MEDIA - PART 02 - Sterilization of media and tools [via autoclaving] --- Going to use the same establishment formula, but doubling the yield. --- Also using RO water instead of distilled water. --- Here's the establishment media formula: --- [» VER.0001 |» INITIAL TROPICAL PLANTS FORMULA |» 0.5L (500mL) RO WATER «] ------ MS [Muashige & Skoog] --------- » 2.22g ------ Sucrose [Cane Sugar] --------- » 15g ------ BAP [6-Benzlaminopurine | Cytokinin] --------- » 0.5mg » 0.5mL » 500μL ------ IBA [Indole-3-Butyric Acid | Auxin] --------- » 0.05mg » 0.05mL » 50μL ------ AGAR [Jelling Agent] --------- » 3.5g ------ PH [Potential Hydrogen] --------- » 5.7 --- [» 1mg/mL CONCENTRATION ratio | 1mg = 1mL | 1mL = 1000μL «] - [12:59]: Time to prep the growth media! --- Made sure to turn OFF the lung-room's oscillating fan. --- Filled a measuring cup with 400mL of RO water. --- Dropped a clean magnetic mixing bar into the measuring cup. - [13:05]: Placed the measuring cup on the magnetic stirrer. --- Turned the unit ON and have the unit on LOW. - [13:11]: Using a precision scale (sensitive to 0.01g) along with a micro-pipette (1000μL capacity along with tips) and disposable (100mL) weigh boats, ------ Added 2.22g of MS [Muashige & Skoog] ------ Added 15g of Sucrose [Cane Sugar] ------ Added 500μL of BAP [6-Benzlaminopurine | Cytokinin] ------ Added 50μL of IBA [Indole-3-Butyric Acid | Auxin] ------ Added 3.5g of AGAR [Jelling Agent] ------ Then filled the measuring cup to the 500mL line before covering the top with plastic wrap. --- Alright! - [13:46]: Let the media mix on the magnetic stirrer for a bit. --- Have the unit set to LOW with a steady vortex. ------ [ » ~20 MINUTES LATER « ] - [14:09]: Okay! Time to pH the establishment media! --- Gently removed the bottom cap of the AC Infinity PH Meter PRO [AC-PHM7]. Placed the cap in the storage case for later. Covered the top with plastic wrap (KCL solution still inside the cap). --- Swirled the probe in a cup of RO water to rinse off the storage solution. --- Shook the excess water out and wiped the unit with a clean paper towel making sure all mineral buildup was removed (kept away from the very tip of the probe). --- Temporarily placed the meter back into the cup of RO water. --- Removed the plastic wrap of the establishment media. - [14:13]: Using the AC Infinity PH Meter PRO [AC-PHM7], --- Took the establishment media's initial pH reading. --- The pH was at 5.14 --- Using the micro pipette, ------ Added pH Up solution. --- Made sure to let the media solution mix for a bit before checking for pH readings. - [14:21]: Adjusted the media's pH until it stabilized to 5.58 --- For this batch, tried to go for a range of 5.4 to 5.8 pH. --- Also glad that I did not need to add PH down. - [14:43]: Poured the pH'd establishment media into separate polypropylene containers. --- Made sure to re-mix the media solution between container fillings. --- Ended up splitting the media solution into 12 containers. ------ 8x » 125mL Polypropylene Bottles [with screw cap lids] ------ 4x » 236mL (0.5pt) Glass Mason Jars [with polypropylene jamming lids] --- Have the lids of all vessels loosely on and set each aside for later autoclaving. --- Also filled 12 empty polypropylene bottles with RO water and placed the lids on loosely (4 bottles are re-used). - [14:58]: Prepped the tools for sterilization. --- Took a new 5.25in x 10in sterilization pouch and added the following tools: ------ 3x stainless steel forceps ------ 3x stainless steel scissors --- Before placing into the pouch, wiped each tool with a paper towel sprayed with 70% isopropyl alcohol. --- Sealed the bag and marked the date. --- Time to autoclave everything in the pressure cooker! --- Filled a Presto 23qt-Pressure Cooker with 12 cups of RO water. - [15:06]: Added a few aluminum foil balls to the bottom of the pressure cooker. --- Placed the first rack on top of the foil. --- Made sure the rack was level and stable resting above the water. --- Gently placed the containers of media into the pressure cooker and onto the first rack. Lids are still loose. --- Made sure to give space for each container and kept them away from the sides of the pressure cooker. --- Placed a second pressure cooker rack on top of the containers of media. --- Placed the containers of RO water onto the second rack. Lids are still loose. --- Placed the third rack on top of the containers of water. - [15:16]: Lastly, placed the sterilization bag of tools on top of the third rack. --- Took the pressure cooker lid and made sure the release vent was clear of debris before placing it on. --- Sealed and secured the lid. --- Pressure vessel has been sealed! --- Alright! Time to sterilize! --- While keeping the vessel level and straight up, moved the sealed pressure cooker to the kitchen for heating. - [15:23]: Placed the pressure cooker onto a butane-gas stove. --- Turned the stove ON and set it to HIGH. ------ [ » ~20 MINUTES LATER « ] --- Once steam was venting out of the release vent, --- Placed the metal rocker onto the vent. --- Shortly afterwards, the pressure top-lock sprung up to indicate the vessel was under pressure. --- Then the pressure gauge started to rise. ------ [ » ~10 MINUTES LATER « ] --- At 15psi, the metal rocker started to rock! - [15:56]: Turned down the stove down to LOW. --- Set a timer for 15 minutes and began monitoring the pressure gauge. --- Maintained the 15psi by making minor adjustments to the stove. ------ [ » ~15 MINUTES LATER « ] - [16:12]: Turned the stove completely OFF and waited until the pressure cooker's top-lock dropped down which indicated that pressure levels were safe to open. ------ [ » ~40 MINUTES LATER « ] --- Once the pressure cooker was at safe levels (0psi) and the top-lock was down, - [16:48]: Moved the pressure cooker into the lung room. --- The pressure cooker was still quite hot so was careful to keep the vessel straight up and level during transport. --- Used towels for insulation. --- Rested the hot pressure cooker onto a stack of 5-gallon buckets covered with layers of towels and folded paper bags. --- Gently opened the pressure cooker and set the lid aside. - [16:57]: While wearing nylon gloves, sprayed my hands with 70% isopropyl alcohol. --- Gently removed the bag of sterile tools placed it onto a Pyrex dish. --- Removed the top rack and set it aside. --- Then one container at a time, --- Gently tightened and secured the lids of each bottle of sterile water before placing onto the Pyrex dish. --- Removed the middle rack and set it aside. --- Then one container at a time, --- Gently tightened and secured the lids of each bottle and jar of sterile media before placing onto the Pyrex dish. --- Throughout the entire process, kept the gloves on and sprayed my hands regularly with 70% isopropyl alcohol. --- Decided to store all the bottles in the Pyrex dish. - [17:16]: Have the dish resting on a shelf in the lung room. The jars of media are set right next to the Pyrex dish on a towel. --- To prevent dust, covered everything with towels. --- Done for now and will check on the growth media tomorrow. --- If all goes well, should be taking new explant cuttings in a couple days! - [17:39]: Night check on the 3x3 veg tent. --- Looking beautiful and healthy in the veg garden! --- All moms are getting bushy and getting pretty close to the grow light. --- Since I plan to take explant cuttings soon, not going to top or train quite yet. --- Clones also looking healthy and seem unaffected by the super-cropping. Nice! - [17:57]: Checked the Ecowitt soil moisture sensor data: ------------------ [ 4x4 MAIN TENT ] --------------- ------ 45% - [CH01 FT » G30 - Heady OG BX1 #1] ------ 34% - [CH02 FB » G30 - Heady OG BX1 #1] ------ 35% - [CH03 BT » G20 - TKO] ------ 55% - [CH04 BB » G20 - TKO] ------------------ [ 3x3 VEG TENT ] ---------------- ------ 43% - [CH05 BL » GR7 - TKO] ------ 45% - [CH06 BR » GR7 - Gorilla Goat #1] ------ 42% - [CH07 FL » GR7 - Heady OG BX1 #1] ------ 44% - [CH08 FR » GR7 - Garlic Budder OG#1] --------- Veg: 38% - 45% | Flower: 45% - 55% --------- General: 30% [Dry] «| 45 - 60% [Moist] |» 70% [Wet] - [17:59]: Checked the 4x4 main tent. --- Ca-Caw!! I got Headies! --- Wow! Heady OG BX1 #1 is drying along beautifully and she is smelling dank! --- Also no signs of mold and RH levels look stable! --- 6 more days until the big trim! - [18:11]: Checked the humidifiers. --- Tanks are still full and no leaks found. --- Wow what a day! --- Still gots more to do! --- Let’s go! Let’s grow! --- Onwards Ensign! Day 05 - Friday - 10/17/25 - Total Days: 187 ----------------------------------- Daily Averages ~[+01:00] - Temps: 76.0°F [24.4°C] - Humidity: 62.0% - VPD: 1.16 kPa - Soil %: [48, 34, 35, 55, 44, 44, 44, 42] ----------------------------------- - [22:57]: Night check on the 4x4 tent. --- Ca-Caw! Headies is smelling dank! --- RH levels are getting a bit dry, but are okay. --- Wow buds are frosty with trichomes! --- With 5 days until trim day, getting stoked! - [23:04]: Checked the Ecowitt soil moisture sensor data: ------------------ [ 4x4 MAIN TENT ] --------------- ------ 47% - [CH01 FT » G30 - Heady OG BX1 #1] ------ 34% - [CH02 FB » G30 - Heady OG BX1 #1] ------ 35% - [CH03 BT » G20 - TKO] ------ 55% - [CH04 BB » G20 - TKO] ------------------ [ 3x3 VEG TENT ] ---------------- ------ 46% - [CH05 BL » GR7 - TKO] ------ 40% - [CH06 BR » GR7 - Gorilla Goat #1] ------ 45% - [CH07 FL » GR7 - Heady OG BX1 #1] ------ 50% - [CH08 FR » GR7 - Garlic Budder OG#1] --------- Veg: 38% - 45% | Flower: 45% - 55% --------- General: 30% [Dry] «| 45 - 60% [Moist] |» 70% [Wet] - [23:11]: Checked the 3x3 veg tent. --- Looking healthy in the veg garden! --- Mothers are getting bushy and tall! --- Super-cropped and trained the taller branches down to lower the canopy. --- Plan to take tissue culture cuttings tomorrow so glad there are plenty of tops to choose. Still deciding on which mother to preserve next. --- Clones in the propagation tray look healthy! --- The original Garlic Budder #2 clone needs watering. - [23:26]: Here is the starting initial watering range: ------ Garlic Budder OG#2 » 256.4g → 300.2g --------- General Solo Range: [Dry] «| [Initial Cup Weight] + ~55-100g |» [Wet] --- Took the starting weight before watering, ------ Garlic Budder OG#2 » 258.9g --- While on a scale, --- Gently misted her straw mulch with RO water. --- Tried to keep the top growth dry. --- Misted 5-10 sprays. Waited a few seconds, then misted again. --- Made sure to reference the watering weights. --- Repeated this process until the cup weighted at least 300g. --- There was no runoff. - [23:40]: Took the saturated weight after watering, ------ Garlic Budder OG#2 » [000.0]g --- Marked the watering date on the side of the cup. Also marked the saturated weight on the back. --- Placed her back into the tent. - [23:45]: Checked the humidifiers. --- Tanks are still full and no leaks found. Day 06 - Saturday - 10/18/25 - Total Days: 188 ----------------------------------- Daily Averages ~[+01:00] - Temps: 76.8°F [24.9°C] - Humidity: 60.3% - VPD: 1.25 kPa - Soil %: [47, 34, 35, 55, 44, 44, 44, 47] ----------------------------------- - [15:19]: Decided to decrease the VPD level a bit. --- Within the AC Infinity App, --- Changed the schedule name from 'Flower [12/12]' to 'Drying [0/24].' Oops. --- For the Main Tent [4x4] controller, --- Adjusted the 'Drying [0/24]' Schedule for the exhaust fan. ------ Decreased the VPD LOW trigger value from 1.5kPa to 1.4kPa --- Adjusted the 'Drying [0/24]' Schedule for the humidifier. ------ Decreased the VPD target value from 1.5kPa to 1.4kPa --- This should increase the relative humidity a bit and will maintain a 60-65% range - [16:59]: Evening check on the 3x3 veg tent. --- Wow the veg garden is raging with healthy growth! --- Even with the lower veg temps, growth has been perky and bushy! --- The TKO mother has been looking a bit on the purple-side, but I plan to increase the temps back after trim day. - [17:23]: Okay! Time to continue the tissue culture journey! --- Checked on the sterilized media, water, and tools. --- Ooo yea! The growth media in the glass jars have solidified nicely! --- The media in the bottles also look great! Found only one that did not gel properly. --- Hmm. The sterile water bottles are not looking sterile. Unlike the first batch (using distilled water), each RO bottle had tiny white particles floating in the water. --- I think this was due to the water source. The RO water used was from the plant reservoir. For the next sterilization, will try using water straight from the tap. Will discard the entire batch and try again later. - [17:34]: Anyways! Time to cut and sterilize another batch of explant cuttings. --- Using the similar protocols as last time, but with a few changes. --- Instead of distilled, using reverse-osmosis (RO) water. --- Also simplifying the workflow and will not be using the sterile water. --- Only taking cuttings from one cultivar. --- Today, attempting to preserve Heady OG BX1 #1 --- Unlike the last attempt, going to be cutting apical nodes and will leave all leaf growth on. --- Last attempt was more for monsteras and not cannabis. - [17:35]: Made sure the lung room's oscillating fan was OFF during the entire process. --- Put on a fresh pair of nitrile gloves. --- Okay! Let's grow Ensign! - [17:47]: Filled a glass beaker with 180mL of RO water. --- Using a micro-pipette, ------ Added 20mL of Clorox bleach --- Mixed the solution. - [17:52]: Poured the bleach solution into a glass mason jar. ------ Added 1 drop of Dawn dish soap. --- Dropped a magnetic mixing rod into the jar before setting it onto the magnetic stirrer. --- Turned the unit ON and set it to a low setting. --- Placed a polypropylene lid loosely on. - [18:02]: Rinsed and filled the glass beaker with 70% isopropyl alcohol. --- Filled another glass beaker with RO water before adding a drop of Dawn dish soap. Mixed the solution. --- Okay! Time to take the cuttings! --- Using a clean pair of trimmers, - [18:09]: Gently cut two clone cuttings from the Heady OG BX1 #1 mother. --- Stripped each cutting down to 2-inch apical nodes making sure to leave about 0.5 inch of stem growth for planting. ------ Prepared a total of 2 nodal cuttings. --- Dropped the nodal cuttings into the cup of soapy water. --- Swirled the cuttings in the soapy water for a bit. --- Opened the jar containing the 10% bleach solution. --- Using clean tweezers, --- Took each cutting out of the RO water and carefully submerged it into the beaker of 70% isopropyl alcohol for about 30 seconds. --- Then transferred the cutting from the isopropyl alcohol straight into the jar of bleach solution. --- Once both cuttings were in the jar, sealed the top with the polypropylene lid. - [18:23]: Flipped and set the entire bleach jar upside down onto the magnetic stirrer. --- Started using the magnetic stirrer. --- But the cuttings were getting hit by the bar so ended up turning the unit OFF. --- Instead, made sure to swirled the bottle every few minutes to agitate the cuttings. --- For a proper sterilization, the cuttings should be agitated in the hypochlorite (bleach) solution for at least 20 minutes. --- While waiting for the explants to sterilize, prepped the still air box. - [18:39]: Unfolded and placed a Safloga still-air box onto my desk. --- Thoroughly misted the inside of the air box with 70% isopropyl alcohol making sure to coat the entire chamber. --- Then isopropyl misted and placed the following items inside of the still air box, ------ x1 » Jar of Establishment Media ------ x1 » Bottle of Establishment Media ------ x1 » Glass-Bead Sterilizer ------ x1 » Roll of Plastic Wrap [pre-cut] ------ x1 » Sterilization Bag Containing: --------- x3 » Stainless Steel Forceps --------- x3 » Stainless Steel Scissors --- Made sure to fully coat the outside of the tool bag and each of the containers with 70% alcohol. --- The bag had a torn corner (Oops), so made sure to spray the inside with a few mists of isopropyl alcohol. --- For the glass-bead sterilizer, ------ Wiped the entire unit with isopropyl alcohol. ------ Before filling the unit with the included beads, misted the heating chamber with a spray of isopropyl alcohol. ------ Misted the glass beads again with a spray of isopropyl alcohol. --- Set the cleaned glass-bead sterilizer into the still air box. - [19:02]: Still air box fully prepped for explants! --- Okay! Explant nodal sterilization complete! --- While still wearing gloves, --- Misted the outside of the explant jar with 70% isopropyl alcohol before placing it into the still-air box. --- Turned the glass-bead sterilizer ON. --- Made sure to re-seal the chamber. - [19:05]: Okay! Finally time to start the tissue culture transplant! --- Let's go Ensign! --- Sprayed my gloved hands with 70% isopropyl alcohol (also put on a pair of cloth arm sleeves), --- Placed my hands through the arm holes and into the still air box to begin the process. --- Started by laying out my initial workspace: ------ » Far Left Quadrant » Jar of Sterile Establishment Media ------ » Far Center » Bottle of Sterile Establishment Media ------ » Far Right Quadrant » Glass-Bead Sterilizer ------ » Mid Center » Bleach Jar with Explant Cuttings [Main Workspace] ------ » Near Left Quadrant » Empty [Trash Area] ------ » Near Right Quadrant » Bag of Sterile Tools & Roll of Plastic Wrap --- Opened the bag of tools and placed the forceps and scissors into the pre-heated glass-bead sterilizer. --- Set the bag in the trash area (near left quadrant). --- Loosened the lids of both containers of sterile media but did not remove the lids. The lids were resting on top and could easily be lifted. --- Also took lid off of the bleach jar and gently set it on the table. --- Took the jar of sterile media and set it in the center of my workspace (to the right of the bleach jar). --- Using my left hand, took a set of scissors from the glass-bead sterilizer and gently cut the bottom of the first cutting. --- Once the bottom cut was made, placed the scissors back into the glass-bead sterilizer. --- Using my left hand, lifted the lid off of the jar of sterile media and inserted the trimmed end of the cutting straight into the growth media (using my right hand). --- Covered the jar with the lid and placed the forceps back into the glass-bead sterilizer. --- Tighten the lid of the jar and set it aside. ------ [ » REPEATED THE ABOVE PROCESS FOR THE BOTTLE OF GROWTH MEDIA « ] --- Once the last cutting was transferred from the bleach jar into the sterile media, --- Turned the glass-bead sterilizer OFF. --- Cleaned up my workplace and placed the scrap stem tips and bleach jar out of the way (near left quadrant). - [19:28]: Wrapped the lid of each explant container with plastic wrap before setting it in the center of the workspace. --- Explant transplant completed! - [19:59]: Using a label printer (Brother DT410), made a set of labels for each container: ------ Heady OG BX1 #1 | 04.24.25 | Photo | Reg ------ Formula: v0001 | ExBatch: 0002 | Lot: 01 | Pod: 01 ------ Establishment Media | Sealed On: 10/18/25 --- Opened the still air box. --- Then one container at a time, took an explant out and wrapped the lid with a label. --- Set the labeled containers onto the propagation tray (in the 3x3 veg tent). - [20:07]: Done! ------ The explants are around 37 μmol/m²/s which gives a DLI of 2.40 mol/m²/d under an 18/6 light cycle. --- Will check daily for contamination. --- Ca-Caw!! Ahh yea! --- Heady OG BX1 is now officially my first true attempt at cannabis tissue culture! --- Using this new workflow, I may try reusing the TKO media. Do plan to take new cuttings soon. May even try using the liquid media. --- Shout out to microclone and Bill Graham! His 'Tissueponics' video contains tips and tricks of the trade that have straight up changed the way I think of DIY cannabis tissue culture. --- Plan to further refine my process and will continue to refine the workflow with each attempt. - [20:15]: Checked the Ecowitt soil moisture sensor data: ------------------ [ 4x4 MAIN TENT ] --------------- ------ 54% - [CH01 FT » G30 - Heady OG BX1 #1] ------ 34% - [CH02 FB » G30 - Heady OG BX1 #1] ------ 35% - [CH03 BT » G20 - TKO] ------ 55% - [CH04 BB » G20 - TKO] ------------------ [ 3x3 VEG TENT ] ---------------- ------ 43% - [CH05 BL » GR7 - TKO] ------ 48% - [CH06 BR » GR7 - Gorilla Goat #1] ------ 42% - [CH07 FL » GR7 - Heady OG BX1 #1] ------ 45% - [CH08 FR » GR7 - Garlic Budder OG#1] --------- Veg: 38% - 45% | Flower: 45% - 55% --------- General: 30% [Dry] «| 45 - 60% [Moist] |» 70% [Wet] - [20:56]: Checked the 4x4 tent. --- Ca-Caw! Looking great my ladies! --- Humidity levels are still a bit on the dry side, but still okay. - [21:08]: Checked the humidifiers. --- Tanks are still full and no leaks found. --- Wow what a day! --- Oh yea, 4-more days until trim day!! Ca-Caw! Let’s go! Day 07 - Sunday - 10/19/25 - Total Days: 189 ----------------------------------- Daily Averages ~[+01:00] - Temps: 76.7°F [24.8°C] - Humidity: 59.4% - VPD: 1.27 kPa - Soil %: [48, 35, 35, 55, 44, 43, 44, 42] ----------------------------------- - [07:08]: Quick morning checked on the 3x3 veg tent. --- The veg garden is healthy and perky! --- All moms and clones are healthy and raging with growth! --- Both Heady OG BX1 #1 explants also look alive and green! - [19:26]: Night check on the 4x4 tent. --- Ca-Caw! We got MENDO DOPE in the trunk! --- Wow terps are getting loud and buds are drying along nicely! --- Looking frosty and coated in trichomes! --- Three more days until trim day!! - [19:33]: Checked the Ecowitt soil moisture sensor data: ------------------ [ 4x4 MAIN TENT ] --------------- ------ 46% - [CH01 FT » G30 - Heady OG BX1 #1] ------ 35% - [CH02 FB » G30 - Heady OG BX1 #1] ------ 35% - [CH03 BT » G20 - TKO] ------ 54% - [CH04 BB » G20 - TKO] ------------------ [ 3x3 VEG TENT ] ---------------- ------ 40% - [CH05 BL » GR7 - TKO] ------ 43% - [CH06 BR » GR7 - Gorilla Goat #1] ------ 47% - [CH07 FL » GR7 - Heady OG BX1 #1] ------ 41% - [CH08 FR » GR7 - Garlic Budder OG#1] --------- Veg: 38% - 45% | Flower: 45% - 55% --------- General: 30% [Dry] «| 45 - 60% [Moist] |» 70% [Wet] - [19:35]: Checked the 3x3 veg tent. --- Vegging along beautifully in the garden! --- Mothers are getting taller and probably could train, but will wait until tomorrow. --- Clones and explants also look healthy and green! --- Gorilla Goat #2 needs watering! - [19:44]: Here is the starting initial watering range: ------ Gorilla Goat #2 » 255.4g → 300.3g and was last watered 5 days ago. --------- General Solo Range: [Dry] «| [Initial Cup Weight] + ~55-100g |» [Wet] --- Took the starting weight before watering, ------ Gorilla Goat #2 » 244.9g --- While on a scale, --- Gently misted her straw mulch with RO water. --- Tried to keep the top growth dry. --- Misted 5-10 sprays. Waited a few seconds, then misted again. --- Made sure to reference the watering weights. --- Repeated this process until the cup weighted at least 300g. --- There was no runoff. - [20:04]: Took the saturated weight after watering, ------ Gorilla Goat #2 » 345.6g --- Marked the watering date on the side of the cup. Also marked the saturated weight on the back. --- Placed her back into the tent. - [21:41]: With a few days before the big trim, decided to sample a few buds! --- Cut and trimmed branch for sampling and wow she’s got terps! --- Buds are dense and have a gassy diesel funk! Ca-Caw!! Wow! --- Without even curing, pretty sure she’s the one! --- Plan to grow out another cycle (in clone of the same pheno), but I think I found my female for my first breeding project! - [22:01]: Checked the humidifiers. --- Tanks are still full and no leaks found. --- Ahh yea! Three more days until the big trim! --- Also next week will be the last for this log. --- But already stoked for the next! --- Planning on popping more seeds and continuing the pheno hunt for another female! --- Since I lost TKO this harvest cycle, plan to grow a clone cutting of the same pheno (#2). --- As for seeds, popping more regulars and going to attempt to grow some of Mr. Grow-It’s Chillout OG! --- Got a pack of F3s and am stoked to give them a try! --- Also going to sprout some beans of Dead Ops OG from Zaza Genetics! --- Chillout OG, Dead Ops OG, and another round of TKO! Let’s go! Let’s grow! --- Hunters of the dank! Ca-Caw! --- Anyways! --- Onwards to next week! [END OF WEEK 26]

Another 8 days have gone by and they still love it. Done some more defoliating and treated with nematodes due to fungusgnat population got to big. Should be a smooth ride from here on.

Blackberry moonrocks both plants are still some of the best seedling in the tent so far. The better if the 2 is for sure the #1 plant in the tent. It has had the best start of a seedling i have seen in a wile. The leaves are angled like an airplane and they just seem thicker than the average leaf. They both seem like there going to have middium to longer indernode spacing. It's looking like a good start to a week the plants are strong they are getting to the point I can let the dried dry out completely. I'm thinking by the end of this week the plants will be starting to be sold. Last week I put all the plants into my bigger 2x4 tent with my medicgrow mini sun 2 the 500w version. Only a few of the plants were ready for that light. Seems like the only plants that really have good resistance and have a strong start are the weedseedsexpress.com seeds. Shout out to weedseedsexpress.com for the strong plants. I ended up putting all the plants back into my 2x2 tent with the 55w amazon light it has alot more blue light in its spectrum. It's kinda weird b4 I switched the plants to the 2x4 tent they were getting 220umol under my amazon 55w led then when I put them under the 500w light 25% strength about 50in from plants and they were getting only about 195umol in that tent but it was stressing most the plants. I assume a larger light has more side lighting hitting the plants. I think when useing larger lights it's good to measure umols from the top but also coming from the sides. I think durring seedling stage they only need about 50% the umols coming from the side the plants as the top is receiving. When I put the plants back in the small tent about the same umols as they were getting b4 the switch and they were still a little stressed. So for a few days I put the small light at the top the tent giving them 100umols for a few hrs then 130umols the rest the day.

hello growmies! day 51 finally the pre-flowers begin to appear. I apologize for the few photos but unfortunately they really filled the box and I find it difficult. overall I have to say they look in perfect shape! I will keep you updated thanks for passing by. like and comment! good day and beautiful growth to you 🌳🌱

D19 (10/12/2020): Beginning of week 3. I saw little yellow/brown spot on big leaves and new leaves, I need to check this closely. I fed with half of the recommended dose but 1.5-1.75L of water per plant. I was giving 1L max and I think it was not enough for the size of my pots. RH of the grow tent is now down at 60%. - temps: 26C - water: PH6.3, PPM: 410 - RH: 60% D20 (11/12/2020): Today I saw thrips larva crawling on a leaf (see video above). That confirm my problem is not a nutrients deficiency. I know how I got them and it's a rookie mistake from me. At the beginning of my grows, I placed a plant in my tent that was originally outdoor during fall. I did not think that it would be infested with bugs. I removed it when I saw a little bugs fly in my tent but the damage was probably already done. I read about thrips and you can get rid of them if you act fast and soon in the infestation. I will go to my local horticulture store today and ask advices and ways to exterminated them. I'm a newbie in growing cannabis (plants in general) and I want the best for my plants. I'm learning from my mistake and that's what I like! Overall, both plants are growing fast and they react really well to LST. I readjust the tie 2-3x a day because the small leaves are growing so fast! - temp: 26C - no water - RH: 60% D21 (12/12/2020): I bought insecticidal soap and gave a treatment. RH is now at 55% and that increases air flow. -temp: 26C - no water - RH: 55% D22 (13/12/2020): The lamp is now 100% and at 28inch form the plants. They received 430 PPFD (33 000 lux). Temperature is under control around 26C with a new oscillating fan place on the floor. This air flow will help against bugs. LST continue every day to expose new leaves. I gave water with only Max Minerals. - temp: 26.5C - water: PH6.4, PPM: 275, approx.: 1,75L per plant - RH: 55% D23 (14/12/2020): LST continue, I try to bend one branch every day and readjust the other ones. I did not see more bugs but some of the new leaves are affected so I know they are still there. I ordered nematodes to help me prevent new larvae. They are suppose to arrive in two days. - temp: 26C - no water - RH: 55% D24 (15/12/2020): I removed leaves at the bottom of the pants last night. Probably 3-4 leaves per plant that were not receiving light. This morning I can see that it gave a good boost on the new leaves! Continuing LST as usual. The nematodes arrived earlier then expected. I will apply them tonight as soon as the lights turn on! - temp: 26C - no water - RH: 54% D25 (16/12/2020): I did a lot today! First, I sprayed another treatment of insecticidal soap on all my plants. Second, I removed one inch of soil in each pot and replaced it with new soil (same soil). I saw sign of what I think is phosphate deficiency. I was really shy on nutrients since beginning of the grow. Third, I fed full recommended dose for week 3 of veg. I gave 1.5L of water with nutrients and approx. 0.5L of tap water when I placed the nematodes. Fourth, I placed one tea pouch of nematodes in each pot as recommended. I also opened 2 tea pouch of nematodes and sprinkled them over the soil of all plants. Finally, I continued LST. It was a big week of up and down. Both plants grew like crazy and I discover an infestation. I think Banana Kush is in pre flower but I'm not sure. It's a bit early but they got so much stressed this week. -temp: 26C -water: PH6.5, PPM: 725 -RH: 55%

Week 8 (9.2225--9.29.25) took photos on 9.24.25

Hey y'all! I'm back with an early update because my biggest two plants are flowering! Hindu Kush looks like she's gonna grow some really thick buds. I'm super excited to see it flower. The GSC pistils are some clear, some amber. It makes me really excited for how she's gonna look as she develops buds. I wanted to give a better tour of how the plants look so I took a video to share as well! I am super excited, and I'm starting to get impatient quite frankly! Lol! But really, I can't wait to watch my plants grow day in and day out. I need to find a way to control the humidity, as it is getting hotter in my home and when my tent is closed the humidity climbs up to 82% and some leaves that touch condensate on each other. Not good! I leave my tent open for hours at a time to tame the humidity but this is not a full time solution. if anyone has suggestions please let me know! I will be asking a question soon on it!

They started blowing up last week we're in the beginning of the 4th week and there looking good spraying them with Dr zymes ones a week

Comments in the video, I'm time challenged so way easier.

The end is in sight, likely in 2 weeks, they are producing compact and sticky buds, looks like it is going to be easy to trim, so little leaves in those buds! They smell fruity, earthy, fuel and a very strong kush base with lemon on one of my pheno, the second one is a little smaller and she smells more like carame&peaches in a kush sauce lol absolute beauties!

Bien aquí esta el día esperado la cosecha, es mi primer cultivo y considero que lo logre. En muchos medios de lectura y en especial en vídeos mencionan que no puedes esperar mucho es tu primer cultivo. En mi caso es falso y lo demuestro ya que si aprendes, investigas por cuenta y lees lo suficiente, obtendrás un alto porcentaje de que los resultados se den. Muy contento con mi plantas y sus tonos morados. Puntos a mejorar tener una carpa de cultivo y por su puesto un extractor de humedad para que baje la humedad preferiblemente a 45 grados Vendrán mejores contenidos y cosechas mas abundantes. Gracias a todos por su vistas y comentarios. Saludos y bendiciones!

She smells and tastes great 🔥🔥🔥🔥she also looks good with her purple buds.

** video in 2 parts Sweet and sour showing interesting gold color on her leaves and some curled tips so i flushed her with canna flush and will dry her for a few days.. i dont know whats the provlem except the cold nights for the last few days. Would like to hear opinions.. Grapefruit from dinafem is amazing smell - very fruity and sweet.. Sweet and sour and spliffs strawberry share almost the same smell but sweet and sour is more hubba bubba chewing gum, intensive sweetnes... Remo chemo still no distincitve smell, just sweet.. amazing trichome production. Ayahuasca isnt purple and smells like a bag of nuts and petroleum. Very odd i am afraid i wont like.her...

Almost time for harvest, can't wait to taste the fruits of my labour........... Hope it tastes and gives me a really good high! But we will find out once I do the review. Happy Growing guys