Nute mix is now 150ml A, 150ml B, 50ml PK13/14, 100ml Rhizo, 25ml CalMag and 25ml cannazyme. Nute mix information PH: 6.4 EC: 2.0 Temp: 21c They are doing far better than I would have thought but the proof's in the pudding. Not that smelly except for lights off.

NYC Diesel Auto (Was Hey Abby) W20 F9 2/1-7 New York City Diesel is ready for harvest. I plan to harvest this weekend, time permitting. I flushed her with 5.5 gal of water since 1/24. Not as much as I would have liked. 2/5 trichome pics show a moderate amount of amber but in some colas, nearly 80%. This maturation was very fast as I was planning for the 15th. Given the amount of amber now showing, I have grown past what I prefer for maturity, this will now lean more indica. We had our first big snow this week following a light dusting last week. Pics show my kitties investigating ( but not for long) Stay green, growers love 💚🌿 💫Natrona💫

Huge growth! Looking super good going to be a thick one!

This week was no less stressful, but the previous week prepared us. More and more snow turns white. The microscope is ready. Nutrient solution at the maximum recommended parameters. But there is a problem with the tips of the leaves, they turn yellow first and then dry up and turn brown. I think this is the consequence of the maximum ppm. direct sunlight, a running giant fan at a distance of 20 cm, and an unstable ph lvl. There were significant changes in temperature from 16 at night to 31 during the day. The funnest is when it rains after a hot day. and they turn off the electricity at night. A humid environment of 65-70% without a fan is my nightmare in reality. So a piece of cardboard and energetic hand movements created the wind and the impression that I crazy But there are often good times, when the temperature is 26-27 degrees and the humidity is 45% without a humidifier. The girls smell amazing 😎

22. Oktober Alle drei Samen sind was geworden :) Gibt nicht viel zu erzählen außer das ich mich freue dass wieder etwas wächst :D 27. Oktober Es ist schon der erste Mist passiert 😅 meine Lampe war leider nur auf 1% Leistung eingestellt und ich habe es nicht gemerkt x) habe vergessen die Ei Stellung im Timer zu ändern. Das Endergebnis ist Spargel der sich kaum tragen kann. Aber noch ist nichts verloren

Increased EC

Bonjour à tous, ces derniers jours ce sont bien passé pour mes petit bébés elles grandissent doucement mais sûrement.C’est la première fois que je travail avec cette gamme de produits (je vous est mis des photos des produits 😉) pour l’instant j’en suis satisfait après, il faut voir le résultat final ce qui est moins facile 😂. Mes petit bébés commence leurs 5ème semaine (je me suis trompé d’une semaine 😕 désolé 😉) trop pressé!!! 😂 de mettre de nouvelles photos 😜. Je vous dis à là semaine prochaine vivement 😉 bonne journée à tous. Je vous remercie 😉.

Girls are doing ok. Will be increasing the nutrients slightly this week as we are now in week 2 of flower. Day 9, roots are super healthy which is key to some good fruits.

Final week of flower for some and we will start the harvest. Also we flushed the plants at the start of the week. All the plants got flushed and will be given the final weeks as required. All looks good and frosty. Lemon Z and Peyote Cookies look out of this world. Gorilla cookies look good but plenty of variation in phenos

1/20 12am Monday Keeping them in veg for another week, they look great. 12pm good 1/22 3am water raised light up a couple inches, still at 48W. 1/23 moved the CBD plant into this grow, its not an Auto. so this is unexpected, I cut her in half topping yesterday. I will be experimenting Witches Wedding in a smaller container, good thing im out of room. 1/24 Witches Wedding experiment worked. I put her in the dirt a little bit sooner because im more confident about how seeds work now.

18/05/21 giorno 57 tutto procede bene le ragazze sono delle vere bombe! Anche in questa ultima settimana stanno continuando a ingrassare e gettare fuori resina...l odore e molto intenso...per fortuna il filtro fa bene il suo lavoro...💚 21/05/21 giorno 60 messo nei serbatoi acqua a ph regolato inizia il lavaggio 💚🌻🌻🌻non vedo l'ora di raccogliere i frutti del mio lavoro...💨😋 24/05/21 raccolto!👍🌱💚🌻 messo il tutto a asciugare...attendiamo con ansia di provare il risultato....🥁

Temperatures ran very high during week 2, I had to invest in a closed loop ac system so I wouldn't lose any Co2 through ventilation. The plant itself is showing signs of heat stress but is beginning to get its legs. The stem is extremely week at the soil line, I'm hoping some strong air movement will fix that problem in no time.

1/12: impatiently waiting for lemon auto to finish up. Still not sure what the northern lights is trying to do, but..the little buds are slowly growing. S l o w l y... 1/14: things are getting out of hand in here. Northern lights is bushy AF again but the budlets appear to be increasing in size. Lemon is getting closer every day. Looks like I've got milky trichomes and the pistils are darkening. Still a bit early, but I'd be lying if I wasn't anxious for the space.. 1/15: I've accepted that I have at least a week ish left on Lemon auto,but she's getting there! I did some rather risky defoliation on northern lights and holy shit, she's got potential. I'm hoping she's just a slow grower and those buds will really fatten up! Perhaps lemon is just a really fast growing plant? I've also decided that since space is limited, my best bet is to probably rotate the plants every other day to allow all bid sites an opportunity for direct light. I'm reigning myself in. Once lemon auto is harvested I need to NOT immediately plant another. I've got some mephosito seeds I want to get going, but I've learned my lesson. This tent fits two 5 gallon mature plants... Uncomfortably. Three is too many 😂 1/17: wondering if northern lights has is it her to fatten up. Gave her a good flush &feed. Popcorn buds or not, I'm invested (#forscience). Lemon auto is still finishing up. Can't get good enough pics with phone to see trichomes. Clearly, I have a minimum of a week left, but I got a digital microscope so I can keep track! Should be here for next update.

Hey guys:) We‘re finally done with this grow, I‘m pretty happy with the results, but sadly I had one more problem I didn‘t realise until the end. Apparently the white truffle hermed and produced pollen. I think it was due to heat stress, I couldn‘t control the temperature this time around and it was constantly over 32c during the day. Didn‘t know that could stress them so much that they produce pollen… Anyways the white truffle is full of seeds, the other 3 seem to be less impacted with the blue zushi looking the best. Overall they produced much less than last time when I had the triploids, but in terms of trichome coverage and smell they seem superior this time around. I‘m looking forward to their full potential once curing is done. In terms of smell, the white truffle is definitely the most unique and my favorite of the bunch, so sad I messed them up, but that‘s how you learn:) On to the next run, this time I‘m going for sativa dominant strains:)

another resin monster from seedstockers, with a very special scent, full of flavors! grinded it smelled like peppermint, and vaped it has a great sweet creamy taste!

So sativa. Braucht n bisschen länger denke ich wie angegeben. Hat jetzt endlich ihren verdienten Platz im Raum 😌

**Encontrarás la traducción a español al final de la descripción** From/Desde: 26/01/19 || To/Hasta: 01/02/19 From day/Desde día: 57 || To day/Hasta día: 63 ----WEEK SUMMARY---- 27/01/19: Video 1 (Homemade CO2 bottle) 29/01/19: Photos 2 to 11 Wonderful crystals over the buds, also these week buds started to be dense. Smell at home it's obvious this week too, not excessive but can't be ignored (smells like heavens). The day 27 i created a CO2 botlle, i can't measure how much the CO2 growed in the room (already ordered a sensor to handmade a wifi CO2 sensor with an arduino), but i suppose it's not too much. I used a 5l bottle with 4l water + 1.600 gr sugar and 4 small packets of yeast (chemical yeast will not work). ----IRRIGATION CALENDAR---- 28/02/19 (Day) I prepared a solution with 3l water and (Silica Power, Fast Food, Auto Stimulator, Bud Candy, Big Bud, Bud Factor X, B52 & Bloombastic) @ 2.0 E.C. PH6.4 ~750ml for each BlueBerry Mazar 31/02/19 (Day) I prepared a solution with 3l water and (Rhino Skin, Sensizym, Bud Factor X, Big Bud, Bud Candy, Pro Active & Bloombastic), EC fix with Fast Food and Auto Stimulator @ 1.9 E.C. PH6.4 ~750ml for each BlueBerry Mazar *****ESPAÑOL***** ----WEEK SUMMARY---- 27/01/19: Vídeo 1 (Creación de botella de CO2 casera) 29/01/19: Fotos 2 a 11 Empiezan a aparecer maravillosos cristales sobre los cogollos y estos empiezan a ser densos. El olor en casa se hace obbio esta semana, no es excesivo pero es difícil no olerlo desde fuera de casa si se abre la puerta (huele de maravilla). El día 27 cree una botella casera de CO2, de momento no puedo medir como ha afectado al CO2 general del armario (ya he pedido un sensor para hacer un medidor de CO2 wifi con un arduino), yo supongo que mucho mucho no ha afectado. Para hacer la botella he usado una botella de 5 litros con 4l de agua, 1.600 gr de azúcar y 4 paquetes pequeños de levadura (orgánica, la química no funciona). ----CALENDARIO DE RIEGO---- 28/02/19 (Día) Preparo un solución con 3l de agua y (Silica Power, Fast Food, Auto Stimulator, Bud Candy, Big Bud, Bud Factor X, B52 y Bloombastic) @ 2.0 E.C. PH6.4 ~750ml para cada BlueBerry Mazar 31/02/19 (Día) Preparo un solución con 3l de agua y (Rhino Skin, Sensizym, Bud Factor X, Big Bud, Bud Candy, Pro Active y Bloombastic), corrijo la EC con Fast Food y Auto Stimulator @ 1.9 E.C. PH6.4 ~750ml para cada BlueBerry Mazar

All content on this diary is for inspirational and educational purposes only. The ideas shared are not a substitute for professional advice. This diary/account is not officially affiliated with Alan Watts or his estate. All materials are used under the principles of fair use. I honor the legacy of Alan Watts by sharing his wisdom respectfully and with the intention of inspiring awareness and self-understanding. 2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

20/5/2018