2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Last few days now, i gave them one feed with canna flush. I’ll just give them ph balanced water for now. Then put them in 48hr darkness with no water. I moved them to my other tent in preparation for harvest.

seeds germinate in rockwool untill roots hit the water i only use calmag and small amount of nutes

(11/25-12/01) Hope everyone enjoys the daily progressions of overhead and side profile (Organized Chaos) Unfortunately they have too much of a toll for me to keep up with with 3 sets of grows going now, so this was the last week I am doing them on this set. I will try to add a video and black back or cover pics by end of week every week. Week 3 Notes & Observations: As mentioned last week, Plant 1 remains the supreme plant now and is producing nice stretch in the node spacing but is still showing a different type of leaf shape than plant 2. No issues from the mainline topping and they seem to be going okay. I will be topping again this weekend for the second of 3 total topping sessions. This topping will be 2 tops off the ends of the last and will produce 4 main colas. **OOPS** was being a little too aggressive in my HST while trying to set the mainline on both plants today. P1 was just a little rip of the skin, I taped her up and suspect she will be fine. However, on P2, I nearly snapped the whole branch off. I taped it and crossed my fingers hoping it works out in a week or two, but have serious doubts it will be able to mend such a large break. We'll see because I am posting ALL of the grow, the good and the bad. VPD and PPFD: This week I will hold the humidity in the tent to about 70-75% and temps will be monitored for 70- 75F daytime and 66-71F overnight. Im not adjusting my lights and look to control them 350ppfd max. Meaning slight increase over last week but mostly just growth increase. Feed & Monitor: ***All feeds with nutes use either a whole ratio or combination of "Veg Mix" and "Bloom Mix"concentrates DILUTED in water until a total ppm of add-in is reached using a (Total Dissolved Solids) TDS Meter measured in PPM (parts per million). The "Veg Mix" concentrate will eventually be added in smaller ratios and "Bloom Mix" concentrate will eventually replace the "Veg Mix" concentrate entirely. The ppm and ratios of each feed will be listed when I feed. Veg mix recipe is on week 2. Bloom Mix recipe will be noted in this top message of the week that I make it.*** Day 28 (last feed was day 22) Tested and Calibrated my ph pens. Starting weight from each pot was 18 lbs and 8 oz, P1 weighed 15lbs 12oz and P2 was 15lbs 4oz before feed. Each plant got 1 gallon of de-chlorinated tap water with 300ppm Veg Mix concentrate added (recipe on week 2 and makes 1 gallon at about 3600 to 4000ppm concentrate to dilute each feed, i.e. I only fed 300ppm above the purified water ppm this feed) The ph on this feed was balanced to 6.0ph to combat the original higher runoff ph from the first runoff feed. After feed P1 weighed 22lbs 3oz (21lbs 2oz after last feed) and P2 weighed 21lbs 14oz (20lbs 14oz after last feed). I got about 5cups of runoff on P1 (9 cups last feed) and 5 cups on P2 (7 cups last feed) . Runoff for P1 ph was 6.45 with 1170ppm (6.45ph with 980ppm last feed) and P2 was 6.45 with 1230ppm (6.45 with 1130ppm last feed). Top soil tested at: P1 6.35,6.3,6.33,6.33 for an avg of 6.327 (6.497 avg after last feed) and P2 tested at 6.47,6.37,6.26,6.51 avg 6.402 (6.46avg after last feed) - next feed will be 6.3ph as Im liking where the ph is so I dont see any issues, but will continue to monitor this way. Day 34 (last feed was day 28) Tested and Calibrated my ph pens. 1st Starting weight from each pot was 18 lbs and 8 oz. Before feeding this time, P1 weighed 14lbs 14oz (15lbs 12oz before last feed) and P2 was 14lbs 7oz before feeding (15lbs 4oz before last feed). Each plant got 1.5 gallons of de-chlorinated tap water with 98ppm Veg Mix concentrate added to flush any salt builds in the soil (recipe on week 2 and makes 1 gallon at about 3600 to 4000ppm concentrate to dilute in each feed, i.e. I only fed 98ppm above the de-chlorinated tap water ppm this feed) Due to this soil showing a possible calcium deficit, I am starting a 1ml per gallon add-in to test on all plants using this soil, so I added 1.5ml of CaliMagic (General Hydroponics 1-0-0) to each plant's feed then I ph balanced before feeding. The ph on this feed was balanced to 6.3ph. I used knitting needles to help both aerate the soil and create new water pathways for the roots. (a practice I may consider a new feeding standard) After feed, P1 weighed 21lbs 9oz (22lbs 3oz after last feed) and P2 weighed 21lbs 7oz (20lbs 14oz after last feed). I got about 11cups of runoff on P1 (5 cups last feed) and 11 cups on P2 (5 cups last feed). Higher runoff volume was expected with the feed volume increase for flush this round. Runoff for P1 ph was 6.65 with 838ppm (6.45 with 1170ppm last feed) and P2 was 6.60 with 879ppm (6.45 with 1230ppm last feed). Top soil tested at: P1 6.58,6.57,6.58,6.67 for an avg of 6.600 (6.327 avg after last feed) and P2 tested at 6.47,6.61,6.64,6.64 to avg 6.590 (6.402 avg after last feed) - next feed will be 6.0ph as I'm still liking where the ph is, but rather see it closer to 6.3 or 6.4 top and bottom. I dont see any issues with the feed's data other than the possible calcium deficit and I will continue to monitor runoff ppms as I expected this to be higher with more runoff. Hope everyone enjoys the daily progressions of overhead and side profile (Organized Chaos) Unfortunately they have too much of a toll for me to keep up with with 3 sets of grows going now, so this was the last week I am doing them on this set. I will try to add a video and black back or cover pics by end of week every week.

Everything’s looking great with these two really happy with these perfect height for me, I’m defiantly going to be doing a lot of Mephisto strains in the near future. ✌️🏻 Still looking good, starting to frost up now also added an 4” air intake and hung it up to blow air across the canopy.

Just letting her do her the now . She looks and smells amazing at the moment.

Dopo circa sei giorni a 12 ore di luce, le parti apicali delle diramazioni hanno iniziato a cambiare forma e a breve appariranno i primi peletti, molto compatte e piene di rami, in ottima salute promettono grandi emozioni! Tra una settimana inizio a rimuovere le foglie più grandi di sotto in modo da fare arrivare più luce.

Our friends at Zamnesia have been working on strains for years and although they are great lovers of experimentation, novelties and know how to keep up with the times like few others, they also know the value of traditions and historical pieces, Blue Dream is one of these, great quality in genetics and stability in fact the plants started very well. In this diary you will find the plant that we will let grow straight, applying only a few techniques that do not involve cutting to preserve its characteristics of speed. After taking the first fertilizers the plant is really doing very very well at a great pace and we expect great things the internodes are growing very well and we are practically almost in pre-flowering. The other one you will find in the next diary worked with Topping / Main Lining / Main Folding Comparison is the salt of experience. We have started the Plagron fertilization program, we are in 100% organic configuration, the soil is recycled Promix + 1/3 fresh soil + 10% Perlite + RQS Mycorrhiza Mix (4gr in the mix, 1 gr under the small fiber pot). We are administering // 1 ml/l of Power Roots - 1 ml/l of Pure Zym - 1 ml/l of Sugar Royal - 3 ml/l of Alga Grow I have doubts about the compatibility of Pure Zym with mycorrhizae. If in doubt, ask the experts, do not mix too much at random. https://plagron.com/en Doubts about Pure Zym / Mycorrhizae compatibility have been dispelled, they can go well together according to Plagron experts. If someone tells you that enzymes eat mycorrhizae, which in principle may seem possible, explain to them that it is not true. https://www.royalqueenseeds.it/growing/452-easy-roots-mix-di-micorrhize.html Try this strain it promises more than good ---- // https://www.zamnesia.io/en/3271-zamnesia-seeds-blue-dream-feminized.html Short Description Zamnesia // Exceptionally tasty and potent, Blue Dream by Zamnesia Seeds is a hybrid with a distinctive and sophisticated character. A blend of Haze and Blueberry strains that manages to bring out the best of both worlds. Its short flowering time of just 9 weeks far surpasses other Haze strains! You can find the whole world of growing at Zamnesia and much more take a tour of the site and you will find "all the best that nature has to offer" in various shapes and colors. The new strains are exceptional and the old ones are no different... p.s. for now zero Hermaphrodites, growers can you say the same? ---- // www.zamnesia.com

The AK loves the Canadian weather cool at night warm during the day just started to show sex she is tall with bog branch spacing

Еще неделька!

Love every plant each one is growing differently stacking different even the visual difference of one strain growin two different ways combined smells of diesel, pez candy and lemons

48 hours of darkness starts today for the last two the smell is more diesel then the first two trichromes look perfect chopping on Saturday The first two smoke sample report after a 6 day dry and week and a half cure earthy diesel inhale and diesel fruity exhale very couch lock half a bout I’m KO definitely night time smoke full report after these two cure thanks for stopping by if you like leave a 👍

This plant just got flushed so all old salts are gone, tommorow i start watering with just finalpart at 3ml/L she will be ready one week later, 10 days max. So next update is harvest.

So this week has been interesting for the gorilla and blueberry. They are packing on some weight especially the gorilla the buds are getting super fat. The zkittlez has been a lost cause for a while now. I decided to cut this down once I started to notice some amber trichs. I'll update and put in a harvest once this is dried and trimmed in about 7 days. There wont be much there. Will mainly make hash from this and edibles. My screens are on the way to make some nice sift. I genuinely think I'll be lucky to pull 3.5gs of dried smokable bud. I am now flushing with plain water both of the remaining plants and I am hoping the gorilla can hold off for another 2 weeks so I can cut these both down at the same time and utilize my tent for drying. These should more than make up for the disappointment of the zkittlez. I had 3 oz in my head as a good yield from this first grow and I'm confident I could make 4 by the looks of these plants. There is some nice fading appearing on the blueberry leaves as you can hopefully tell but they defo need to fatten up. Trichs are half cloudy on both plants maybe slightly further on the gorilla.

Day 89 - pH thing has been quite steady the last few days, but I think part of the reason is I have been keeping it over 6 (between 6.1 & 6.3) which makes me think it may not be able to feed on something so keeps pH stable (This pH issue could be root acidification with too much Potassium; it seems to coincide with the PK Warrior being added - pH needs to be below 6 for Potassium uptake to happen so I artificially blocked it by keeping pH above 6!!!). EC also going up but not as quick as it was...drops back further than it was when adding RO water (i.e. if it drank 10L and EC went from 1.7 to 1.9, adding 10L back takes it to 1.6). So, as I have been promising myself for the last couple of days, I did a full tank change. Have dropped the feed substantially for the start of week 7 (Which is technically Monday coming). New Nute Strength: Silicon - 1ml/L Sumo Boost - 2ml/L Bloom A - 1.2ml/L Bloom B - 1.2ml/L PK Warrior - 0.4ml/L Sensizym - 2ml/L Fulvic - 2ml/L EC 0.8, pH 5.9 Did have A & B @ 1ml/L but EC was barely 0.7 (Background EC of water is 0.2) so added the touch more A&B so as not to super starve them. Will now monitor pH/EC for the next few days and try to keep levels nice and even for the next 9-10 days before going to week 8 force and week 9 flush and the finishing bulb! ************************************************* Day 85 - Dayum this pH management getting funky now!!! For the last week, pH keeps taking a nose dive (0.5-0.7 a day) with water level dropping and EC rising. Can't work out what the girls are at at all. Apart from lower leaf loss due to light being blocked, the plant is creating some new leaf matter (As you would expect) where light is getting reflected down low but can't work out the pH drops. No matter what EC i set them too, or pH, they only appear to be taking on water...or something micro (Ca/MG or the likes) and leaving the macro's. Topped up tank with some RO (Reclaimed from dehumidifier) water and only added calmag since tank change 3 nights back (It has some N in it for the new green and hopefully just gives what it needs with Ca/Mg). Apart from that, just keeping an eye on things to make sure no burn...dont want to put them on a starve on the beginning of Flower week 6!!!

This week repot to 3L and change spectrum

What's up guys...                    The smallest of the three COOKIES sisters has already been in a 12/12 flowering phase for five days...                                She is starting to find out how to get her flowers as beautiful as those of the three landlords, the PEYOTE CRITICAL, which you can find in two other diaries...                At the time of the photos, I gave her 1ml of fish-mix for the first time , in order to give her some extra energy for the pre-bloom phase..                                             PH around at 6.5                     See you in a week to see how much has changed..                     For now the smell of cookies is still far away...but the oven is heating...so I keep some for you😉🍪..                                       If you like the idea, let me know 😁..                                      Bye  everyone and all your plants✌️

Getting bushy now couple have shown sex. Going quite well just a few caterpillars around the garden occasionally. Hopefully have most in greenhouses before flowering starts properly.

This run didnt went the way I expected, loosing half tent yield because that freack plant (46 gr) frostiest and densest the the big one though, well thats life Overall very happy with bud quality