On the first day of this week, I topped the plants after the 5th node. 3 days later I switched to 12/12. The reservoir wasn't quite empty yet, so I didn't fill it this week, but I probably will at the start of the next one. Plants are growing quickly now, the yellowing in the BJ and NYC has also pretty much recovered, nutes seem to be fine at the moment. I fear they might grow taller than I'd like them to, but I guess we'll see. I just hope I don't have to put in the net this time, as I like to reserve the option of taking them out of the tent. I also bought an RO filter, which I am probably going to use the next time I fill the reservoir.

Lemon Cake week 18 7/28 - 8/4-10 I am late for my outside ladies diaries updates. Life has been getting the best of me lately. I’ve been packing my daughter’s room and moving her things to her new home. For those that have followed my since my beginning here, Cancer left me with neuropathy and arthritis in my hands. I can no longer do my glass fusing or flame work. To close this chapter of my life, I’ve been inventorying glass, tools, equipment, molds and supplies to sell. The most stressful has been appealing Medicare claims and receiving the second denials on the same claims. They are the last payer of record so good luck getting any reimbursement you are entitled to. My apologies to all of you for being so brief on your diaries and not providing many comments. Photos are from 7/28- 8/9 for both weeks. For my outside ladies, Opium, Auto Opium, Fractal, and Lemon Cake not much changed. They continue to live in sweltering conditions. Temps are in the upper 80-90s with humidity over 105. In addition to the heat, Hurricane Debby started up the coast but was downgraded to a tropical storm by the time it got to us. Expecting our normal thunderstorms, I waited til morning to bring the ladies inside. Overnight, we had wind gusts of 25mph. Yeah, then I brought all gals into the house. The photo gals are strong and with thick stalks were able to withstand the winds. I top dressed all ladies with Bloom Soil for organic fertilizer. Buds are fattening up, trichomes are minimal at this time but some fragrance is starting to be noticed. Thanks for the visits, likes and comments, I appreciate all the plant love💚. Have fun & love what you grow 💚 Sending you good vibes of love, light, and healing 💫 💫Natrona 💫

Hello everyone friends farm Welcome Back ! We lowered this beautiful girl's apex peak!

Day 78: This week I only gave them water with regulator, humic-blast and ezymen because they where ready to harvest. Those others from last week where kept inside a dark room for some days before cutting them down. By doing this, your plants will increase making thc. In the middle of this week i also putted some plants inside the dark/drying room to make them ready to harvest. These will also be in the dark for a few days before I harvest them. At the end of this week all other plants are in the dark as well for a couple of days, i will keep them in the grow room because there is no point to move them to the other room lol. Because i put them into the dark for days instead of cutting them down, I can cut them fresh and put them in drying nets instead of putting the full plant upside down on ropes. I will use a cutting machine for this.

The Mandarin Squeeze sisters are doing okay. The neem oil treatments have them a little stressed. I am not sure if the issue is solved if not. I will have to go buy a insecticide soap or something for whatever the neem is not eliminating. Just not sure what. I just know it has to stop prior to flowering. 🤞🏻it is solved. Thank you Terpyz Mutant Genetics, and Spider Farmer. 🤜🏻🤛🏻🌱🌱🌱 Thank you grow diaries community for the 👇likes👇, follows, comments, and subscriptions on my YouTube channel👇. ❄️🌱🍻 Happy Growing 🌱🌱🌱 https://youtube.com/channel/UCAhN7yRzWLpcaRHhMIQ7X4g

NOTES: Keeping pH at 5,8-5,9 through the entire Vegetation-phase. EC is now around 1,2 and i'm increasing it if needed. I'll be checking the levels regularly from this week since all of the hydroponic systems are now running. I'm going to add some ice cubes in to the nutrient tanks to cool nutrient solution a bit always when I remember to, even though temperatures haven't been an issue so far. Can't do any harm so why not I quess. I'm also adding a CO2-bag in to the tent above the plants. Also I'll begin training my plants this week by topping them. Day 22 (8.11.) Lights are set to 70%, water pumps are now on for 24/7 (EBB's drain water is on lowest setting possible to avoid drowning the roots) and pH is 5,8-5,9 / EC 1,2-1,3 on every hydroponic system. I topped all of my plants and cut off all of the lowest leafs with single tips and some of the small nodes starting from them. Day 23 (9.11.) pH-level keeps on raising so I'm gonna be measuring it pretty often. I'm correcting it by adding some pH- while filling up the nutrient tanks with small amounts of fresh nutrient solution at a time. Everything's going well and I can't wait to flower these! Day 24 (10.11.) Lights are now set to 80% power at 70-75cm height from the tops. I cut off the rest of the lowest nodes which I had left to some of the plants while topping them. I don't think they would get enough light when they are bigger. Day 25 (11.11.) Removed the lowest (three-pointed) leafs of each plant. Day 26 (12.11.) I moved the Led's a bit higher (now at ~90cm above plants) and set them up to full power. Plants are now around 11-14cm height and growing like crazy! Day 27 (13.11.) The growing has exploded and these plants are getting bigger and bigger. I'm going to defoliate them lightly and remove the lowest nodes so the plants have around 6 nodes each going forward, hopefully I'll get an even canopy. Day 28 (14.11.) I changed new nutrient solution in to the EBB-flood system which has the Ayahuasca's on it. I'm keeping the EC at around 1,3-1,4 and pH at 5,8-5,9 on every system. Also added small amount of Cal-Mag in to both NFTs.

Week 3 day 16, topped today & will start mainlining her, going good.

After 2 weeks I chose my favorite plant of the three and prepared her for the Autopot. Now she needs to root this pot for another 2 weeks before we can turn on the Aqua Valve and the tank. DLI 30

Big stretching...i think too far from the lights. she has begun flowering and smells good. Not getting orange, more lemon. She is leggy and I live how her leaves grow.

April 20-26 This week was a Frost explosion. Smells like you just walked into a candy store. Resin coverage extends into the fan leaves and the trichomes are a solid milky white. It's probably moving from the bulking phase into the ripening phase. I regret not doing any defoliation because the lower buds aren't where they should be but fine. 5 gallon pot in 2x2 is not enough space to bend branches to the max. I'm still satisfied.

live now! FLOweek 6day41 she so cool! #fallen angel #clones #thaiweed #420bkk #livingsoil #organic #growergreenroom #ganja

Starting week 2 of flower. Slowly trimming branches that reach far from the main cola stem.

Thursday 16th march day 59 Harvested a couple days earlier then planned as my humidity was getting a bit high and worried about risking mold I decided to chop, have to get a good dehumidifier soon, everything looking lovely, nice and dense with very strong fruity potent smell definitely the best looking plants I have grown to date not the biggest buds but they look quality, chopped plants whole gonna do my best to keep the temp and rh% in the dry room as close 60/60 as I can to get a nice slow dry, 🍁😎 I only took the wet weight from 1 plant back left plant was 270g with large fan leaves removed

5ª SEMANA DE FLORACIÓN. COGOLLOS YA COMENZANDO A ENGORDAR. PARAMOS CON EL BIG BUD. AUMENTO DE LA POTENCIA DEL LED AL 100% PARA QUE ENGORDEN AL MÁXIMO LAS FLORES.

A few more days and its a wrap on these two gorgeous phenos.

She's showing signs of a phosphorus deficiency. will feed the plant fox farm big bloom to correct the issue.

Welcome to Flower Week 5 of Kannabia Zoap Rosé I'm excited to share my grow journey with you from my Kannabia Seeds Project . It's going to be an incredible ride, full of learning, growing, and connecting with fellow growers from all around the world! Check out Kannabia Seeds with my link [https://www.kannabia.com/de?ref=61966] and grab the germination device or the strains I used . Trust me – it’s worth it for sure ! Get another 20% Discount at all products using the code [GGD] at the checkout. For this Project , I’ve chosen the Feminized Photo Strain Zoap Rosé : Here’s what I’m working with: • 🌱 Tent: 225x150x150 • 🧑‍🌾 Breeder Company: Kannabia Seeds • 💧 Humidity Range: 45 • ⏳ Flowering Time: 56-63 Days • Strain Info: 22%THC • 🌡️ Temperature: 26 • 🍵 Pot Size: 20 • Nutrient Brand: Hy-Pro • ⚡ Lights : 600W x 2 A huge thank you to Kannabia Seeds for allowing me to try my Best with this amazing collection from Photo Strains they managed to Sponsore . Big thanks for supporting the grower community worldwide! Your genetics and passion speak for themselves! Let’s grow together – and don’t forget to stop by again to see the latest updates! Happy growing! Stay lifted and stay curious! Peace & Buds!

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

The start of flower wars very good, we got the first flower nutrients. It will be a Monster plant again

Fifth week and booming I wanna say chop next week but might hold out extra two weeks start flushing yesterday en she's pack with trics sticky as hell well happy I bought purpanater it's the don swer by it now