💜 8×8 Adventure – Week 12 Purple Haze by Zamnesia – Pheno B The Little Frosty Queen Is Still Shining… Even When She Hides From The Camera 😂❄️💜👑 Welcome back to another chapter of the 8×8 Adventure! 🌱💚 And... well... I have a small confession to make. 😂📸 Somehow... SOMEHOW... I managed to miss the individual photographs of our Purple Haze Pheno B this week. Again. 🤦‍♂️😂 I know. I know. After all the talk about how much I love documenting these girls, here we are... with no dedicated photo stack of our little Frosty Queen. Apparently, she decided: "You've photographed my sister enough. This week, I'll be mysterious." 😂💜❄️ But before anyone thinks she's disappeared... she hasn't. She's right there in the room. She's growing. She's beautiful. She's developing. She's doing exactly what she has been doing throughout this entire adventure. And thankfully, we do have general room photographs and a video showing the whole 8×8. So if you look closely... you can still find our little Purple Haze girl in there. 👀💜🌿 And I may still find the missing photographs. I've been digging through different folders because I genuinely believe I took them. So... if they suddenly appear from some mysterious photographic dimension... I'll come back and update this week with her individual photographs. 😂📸🕵️‍♂️ For now, though... we work with what we have. And honestly? Even without the dedicated portraits... I know this girl. She's still beautiful. She's still shining. She's still growing. She's still doing her thing. She's simply doing it a little slower than her sister. And that's perfectly okay. So let's give our little Frosty Queen the spotlight anyway. ❄️💜👑🌿 ──────────────────────── 🌱 WEEK 12 – THE GIRL WHO DOESN'T NEED TO RUSH ──────────────────────── Purple Haze Pheno B continues to be exactly who she has shown us throughout this adventure. Compact. Beautiful. Frosty. And... slow. 😂💜 Especially when you put her next to her sister. Purple Haze Pheno A has apparently discovered the accelerator pedal and refuses to let go. 🔥💜🚀 Pheno B? She's driving with the windows down and enjoying the scenery. 😂 And that's okay. Because when I look at Pheno B, I don't see a plant that's failing to keep up. I see a plant following her own rhythm. She's developing. She's flowering. She's continuing to build. She's maintaining her beautiful appearance. And she's slowly becoming more substantial. She's simply doing it on her own timetable. That's one of the most important things this 8×8 Adventure continues to teach me. Not every plant needs to move at the same speed. ──────────────────────── 💜 SMALLER THAN HER SISTER – BUT STILL A BEAUTY ──────────────────────── There's no question about it. Purple Haze Pheno B is smaller than her sister. And right now, she's also somewhat behind her in visible flowering development. But that's not a criticism. It's an observation. And there's a huge difference. Her sister is moving extremely quickly. The flowers are becoming enormous. The week-to-week changes are dramatic. Pheno B is taking a different road. She's more compact. She's slower. She's developing at a more measured pace. And yet... she's beautiful. That's what matters. Because if I walked into the room without knowing which plant was which, I wouldn't look at her and think: "What's wrong with this plant?" I'd look at her and think: "There's our little Purple Haze." 😂💜🌿 She has her own appearance. Her own structure. Her own rhythm. Her own identity. And that's exactly what we want to document. ──────────────────────── ❄️ THE FROSTY QUEEN IS STILL FROSTY ──────────────────────── If there's one characteristic that has remained impossible to ignore with this girl... it's the frost. ❄️❄️❄️ Even without this week's individual macro session, I know what I'm seeing when I look at her in the room. That beautiful resin-covered character is still there. The flowers continue developing. The surrounding sugar leaves continue carrying that frosty appearance. And when the room lighting hits her from the right angle... she catches the light beautifully. She's not the biggest girl. She's not the fastest. But she has something that makes you stop. You see her. You look closer. And there she is. Our little Frosty Queen. 😂❄️👑 That's her identity now. And honestly... I wouldn't change it. ──────────────────────── 👭 TWO SISTERS – TWO COMPLETELY DIFFERENT CLOCKS ──────────────────────── The contrast between Purple Haze Pheno A and Pheno B continues to be one of my favourite things about this project. Same genetic line. Same room. Same lighting. Same general environment. Same irrigation philosophy. Same nutritional foundation. And yet... completely different development speeds. Pheno A is flying. 🚀🔥💜 Pheno B is cruising. 🚗💜😂 And that's exactly why having both in the same room is so valuable. If I only had one Purple Haze plant, I might look at her development and think: "This is how Purple Haze behaves." But because we have two phenotypes sharing the same environment... we can see that there isn't necessarily one single expression. There are differences. There are personalities. There are rhythms. There are individual plants. And Pheno B is giving us a beautiful example of that. ──────────────────────── 🌿 SLOW DOESN'T MEAN STOPPED ──────────────────────── This is something I want to repeat because it is important. She's slower. She's not stopped. 😂💜 There's a massive difference. She's still developing flowers. She's still changing. She's still growing. She's still using the environment. She's still building. She's simply doing it more slowly than the girl standing beside her. And honestly? That's okay. Plants don't read our weekly schedules. They don't know that we've labelled something "Week 12." They don't look at the diary and think: "Shit, better hurry up." 😂📖🌿 They have their own biological timing. And this girl has made her timing pretty obvious. She takes her time. She does her thing. And eventually... she gets there. ──────────────────────── 📸 THE GREAT PHOTO HUNT ──────────────────────── Now... let's talk about the elephant in the room. 😂📸 Where the hell are her photographs? I genuinely think I took them. I've been looking through folders. Different folders. Old folders. New folders. Folders that apparently contain everything except the photographs I'm actually looking for. 😂🗂️📸 So I'm still hunting. And if I find them... I'll come back and update this week's diary with her individual photographs. Because she deserves them. But for now, we have the general room photographs. And we also have a video showing the entire room. And she's in there. You can see her. You can find her. You just need to play: "Where's Purple Haze Pheno B?" 😂🔎💜 It's basically a grow-room version of Where's Wally. Except Wally is a frosty little Purple Haze plant. 😂💜❄️ And honestly... I'm sorry, girl. You deserved your own portrait session this week. ──────────────────────── 🎥 SOMETIMES THE WHOLE ROOM TELLS THE STORY ──────────────────────── The funny thing is... although I always prefer having dedicated photographs of each phenotype, the general room photographs and video actually give us something different. They show context. They show where she sits. They show her relationship with the rest of the canopy. They show the scale of the room. And they remind us of something important: This isn't twelve separate grows. It's one 8×8 environment. Twelve individual plants living inside it. And Purple Haze Pheno B is part of that larger picture. She's there. She's growing. She's developing. She's simply not standing in front of the camera this week saying: "Look at me." 😂 Maybe she's shy. ──────────────────────── 💡 SAME PHOTONS – DIFFERENT RESPONSE ──────────────────────── The environment hasn't changed specifically for her. The upgraded lighting remains in place. The two additional Future of Grow Black Series 600W fixtures continue providing the extra lighting capacity. We've kept the output controlled rather than simply running everything at maximum. We're still working around: 💡 ~59% power from the plug with approximately: ☀️ ~1,500 PPFD across the upper canopy and naturally varying intensity according to height and position. And Pheno B is receiving those same photons. Just like her sister. Just like the rest of the room. But look at the result. Her response is different. Her development speed is different. Her structure is different. Her timing is different. And that's the beautiful part. The photons are the same. The plant's interpretation isn't. 😂☀️💜 ──────────────────────── ☀️ MORE LIGHTING CAPABILITY – CONTROLLED ──────────────────────── I continue to really like the additional lighting setup. The point was never simply: "Let's make the room as bright as physically possible." 😂☀️🔥 The point was to increase our capability. More available lighting. More flexibility. More control. And then find the level that makes sense for the room. The additional Future of Grow fixtures gave us that headroom. We don't have to run them flat out. We can control the output. And that means we have more capability without automatically turning that capability into wasted electricity. Purple Haze Pheno B gets the same opportunity as everyone else. What she does with that opportunity? That's her story. ──────────────────────── 🌸 THE FLOWERS ARE STILL MOVING ──────────────────────── Even without the dedicated photographs this week, the general room images and the video show us something important. She's still developing. She's not suddenly standing still because her sister is moving faster. She's still part of the flowering progression. The structure is continuing to develop. The flowers are continuing to gain definition. And her overall appearance remains beautiful. That's what I want to emphasize. We shouldn't confuse: "not moving as quickly as Pheno A" with: "not progressing." Those are completely different statements. Pheno B is progressing. She's just doing it at her own speed. And that's okay. ──────────────────────── ⚗️ NUTRITION – NOW WE START EASING BACK ──────────────────────── As we move further into the later stages of the flowering cycle, we're also beginning to change the nutritional approach. And this is something I personally prefer to do gradually. I don't like the idea of working with mineral nutrition and suddenly going: "Right. Zero everything tomorrow." 😂 That's just not how I prefer to approach the transition. Instead, I like to start reducing the nutritional input progressively as the plants move toward their final stages. Small steps. Controlled changes. Watching the plants. Allowing the transition to happen rather than slamming the door shut overnight. And that's what we're beginning to do across the room. Pheno B is included in that gradual transition. We're not trying to force her because she's slower. We're simply allowing the entire room to move forward while continuing to observe each plant individually. Her development may be slower than her sister's... but her needs are still her needs. And that's something I always want to respect. ──────────────────────── 🌡️ WEEK 12 – ROOM CONDITIONS ──────────────────────── The room continues to remain broadly stable around: 🌡️ Day temperature: ~26.5°C 🌙 Night temperature: ~26°C 💧 RH: ~61% 🌱 Substrate temperature: ~25°C 💦 Nutrient solution: ~25°C ⚗️ pH: ~6.02 💨 CO₂: ~693 ppm 💡 Light schedule: 12/12 💧 Irrigation: ~1.45 L/plant/24h ? Pot size: 15 L The important thing here is that the room remains the room. We're not changing everything because one plant is slower. We're maintaining the overall environment while paying attention to the individual responses. And Purple Haze Pheno B continues to tell us: "I'm good. Just give me time." 😂💜 Fair enough, girl. ──────────────────────── 🌿 DOING LESS – SEEING MORE ──────────────────────── This is becoming one of the biggest themes of the 8×8 Adventure. Earlier in the grow, there was plenty to do. Training. Canopy management. Defoliation. Positioning. Adjustments. Decisions. Now? A lot of the job is observation. And I actually love that. Because when the plants are healthy and developing, you don't need to invent work. You need to pay attention. Look. Measure. Listen. Watch. And if something is needed... respond. Otherwise? Let the plant grow. Purple Haze Pheno B doesn't need me constantly touching her. She needs time. And she's getting it. ──────────────────────── 💜 HER SISTER MAY BE FLYING – SHE'S STILL SHINING ──────────────────────── This is probably the biggest thing I want to leave with everyone this week. Yes... Pheno A is absolutely flying. 😂🚀🔥 Her flowers are becoming enormous. Her weekly changes are dramatic. She's moving incredibly quickly. But that doesn't make Pheno B less interesting. Quite the opposite. Pheno B gives us the comparison. She's showing us another expression. Another pace. Another architecture. Another flowering rhythm. And when you put both girls into the same room... that's when the experiment really comes alive. One says: "Let's go!" The other says: "Relax." 😂💜 And both are beautiful. ──────────────────────── 🔬 THIS IS WHY THE 8×8 ADVENTURE EXISTS ──────────────────────── This is exactly why I wanted to run these plants together. Not to prove that one phenotype is better. Not to make every plant behave identically. But to watch what happens when different plants receive a shared environment. The room becomes our constant. The genetics become the variable. And the plants provide the answers. Purple Haze Pheno B is giving us a particularly interesting answer. She's smaller. She's slower. She's frosty. She's compact. She's beautiful. And she's completely herself. That's enough. More than enough. ──────────────────────── 👑 THE LITTLE FROSTY QUEEN STILL DESERVES THE CROWN ──────────────────────── Even without the photographs... I'm not taking the crown away from her. 😂👑❄️ Because she continues to shine. She's not the biggest girl. She's not the fastest. She's not trying to compete. But she has her own presence. She has her own look. And when you know the plants well enough... you can spot her. You know her structure. You know her rhythm. You know that frost. You know that little compact personality. She's become recognizable. And that's one of the coolest parts of following a plant for twelve weeks. You stop seeing a label. You start seeing a personality. Purple Haze Pheno B. The little Frosty Queen. 💜❄️👑 ──────────────────────── 🔮 WHAT COMES NEXT? ──────────────────────── More development. More flowers. More resin. More evolution. And hopefully... more photographs. 😂📸 I'm going to keep looking. And if I find her missing photographs hiding somewhere inside the chaos of my folders... they're coming straight into this diary. But even without them, the journey continues. We'll keep watching her through the general room photographs. We'll keep watching the videos. We'll keep watching the plant itself. And most importantly... we'll let her decide her own pace. No rushing. No forcing. No pretending she's moving at the same speed as her sister. She's not. And that's perfectly fine. ──────────────────────── 💭 FINAL THOUGHTS – STILL SHINING WITHOUT THE SPOTLIGHT ──────────────────────── Week 12. And Purple Haze Pheno B? She's still here. 😂💜❄️ She's still beautiful. She's still developing. She's still frosty. She's still growing. She's still doing her thing. She's simply doing it at a slower pace than her sister. And this week... we somehow managed to forget her photographs. 😂📸🤦‍♂️ My apologies to everyone following her diary. And especially to the little Queen herself. If I find those photographs hiding somewhere in the endless folder jungle... I'll update this week. Until then, we have the general room photographs and the video showing the whole 8×8, where our little girl is still there doing exactly what she's supposed to be doing. And honestly? That's what matters. Because the plant doesn't stop being beautiful just because I forgot to take her portrait. 😂💜 She's still shining. She's still evolving. She's still catching photons. She's still building flowers. She's still showing that beautiful frosty expression. And she's still giving us one of the most interesting contrasts in the entire room. Her sister is flying. Pheno B is taking the scenic route. But both are telling us something valuable. Same genetic family. Different expression. Same room. Different rhythm. Same photons. Different response. And that's the 8×8 Adventure. 💜🌿❄️👑 So keep shining, little Frosty Queen. Take your time. Keep building. Keep doing your thing. We'll be watching. And next week... I promise I'll try to find the damn camera roll. 😂📸 ──────────────────────── 🙏 THANK YOU ──────────────────────── As always, thank you so much to everyone following the 8×8 Adventure. 💚🌱 Thank you to GrowDiaries for giving us the space to document these individual journeys and share these stories with growers around the world. Thank you to Zamnesia for the Purple Haze genetics and for giving us the opportunity to discover two such different expressions from the same genetic family. Thank you to Plagron for supporting the room with the growing media and nutrition throughout this adventure. A huge thank you to Future of Grow for supplying the additional Black Series 600W fixtures and allowing us to explore this expanded lighting setup in the 8×8. 💡☀️🌿 Thank you to Lumiflora for helping bring additional light into the deeper and lower areas of the canopy. Thank you to TrolMaster for helping us monitor and manage the environment behind the scenes. And finally... thank you to everyone following along. Every comment. Every question. Every message. Every person stopping by to watch these girls develop. It genuinely means a lot. 💚 Even when I occasionally forget to photograph one of them... 😂📸🤦‍♂️ This adventure continues to teach us something every single week. Twelve plants. One room. One shared environment. And twelve completely different stories. Purple Haze Pheno B is writing hers slowly. Quietly. Beautifully. And with plenty of frost. 💜❄️👑 So let's give her the spotlight anyway. Let's let her shine. Let's let her take her time. Let's keep those photons flying. And let's see what this little Frosty Queen decides to show us next. And if I find those missing photographs... you'll be the first to know. 😂📸💜 Growers Love, my friends. 💚🌱 See you next week in the 8×8 jungle... where one Purple Haze sister is apparently racing toward the future... while the other is still enjoying the scenery. 😂💜👑❄️ And honestly? I wouldn't have it any other way.

Well I found out that she is having a bit of Cal Mag defficiency thats why the leaves are getting too yellow and fast. But so far so good. I hope she stays all good. I think this week she will be ready to harvest.

Las flores se desarrollan perfectamente aunque la 8 ball al ser 100% indica se va a quedar bastante atrás pero las red mandarin me tienen sorprendido para bien, sobre todo la de color rojo que está preciosa. En la próxima semana mostraré los días 9, 11 y 13 de noviembre, ayer dia 9 empecé con el engorde de boom nutrien y a partir de ahora un riego con abonos con las cantidades que lleva y el siguiente riego solo la mitad de nutrientes además de ser la última semana que le hecho el bio-grow para dejar solo el bio-bloom. También quité la malla por última vez para hacer un poco de limpieza y las cambié de sitio además de levantar un poco más la maceta de la 8 ball para que quedara un poco más a la altura de las otras mañana dia 11 grabaré un video para ver como siguen y el día 13 otro aunque el riego creo que será el día 12 que son de 750ml por maceta cada tres dias aprox.

She’s budding up nicely finally done stretching and starting to smell strong

Sie wächst super und bekommt endlich diese Woche ihren ersten Dünger. Diesmal Dünge ich wieder mit meinen Favoriten vom MaryJanes Growshop.

RESUMEN DE CULTIVO: Esta cepa es brutal, resiste muy bien las manipulaciones y genera cogollos grandes y prietos, también soporta una fertilización poco controlada y ligeramente excesiva. Perfecta para alguien con pocos conocimientos (este es mi primer cultivo) OLOR: No tiene un olor fuerte, una buena opción para quien necesita disimular el olor del cultivo. SABOR: Su perfil de terpenos es muy bueno pero suave, varia en funcion de la planta, algunas tenían un sabor ligero, especiado y tirando a lo picante pero una en particular si sacó matices de olor y sabor muy dulces y afurtados entre el mango y el platano realmente buenos, me encantaría tener una madre de ese ejemplar. EFECTOS: Tiene altos porcentajes de THC, a tumbado a más de un amigo, me parece una buena opción si buscas una variedad potente. LO MALO: Poco malo tengo que decir, aunque tampoco es tan sabrosa como me hubiera gustado. Seguiré buscando otras variedades pero seguro que repito.

This plant will be grown again for sure. From start to finish there wasn’t a single issue and she performed well under my Optic LED and LST. Can’t wait to grow this again.

Strawberry 🥧 I feel like her milky trichomes were starting to turn a little amber on some of the more mature buds so I chopped her down.Indica dominant strain so I wanted to let her go as long as possible.Smells great I’ll update a smoke report after she dries and cures.I think I’m going to use the majority of this one for some Rick Simpson oil and see how she turns out :)

Empecé la germinación el 23/01/23 en un vaso de agua durante 12 horas, después al taper con servilleta húmeda 24 horas más. Radícula de 2cm y las planto en maceta de 1L Actualización estado de germinación 03/02/23: Meto fotos ya con las macetas rellenas al 10º día y fotos del 7º día comparando las macetas y los tallos. En cuanto el 2º par de hojas sea mas que evidente empezamos las semanas de crecimiento. Dentro de 2 o 3 semanas quizás, trasplantamos a maceta definitiva de 7Litros. Lo veremos. Actualización 06/02/23: Meto unas fotos de las plantas mas grandes, 3 días después para que se vea la diferencia de crecimiento. Además un video mostrando el cultivo entero, la luminaria y la extracción. Llevamos 13 días desde la germinación. Actualización 08/02/23: A partir de aquí voy a empezara a contar ya las semanas de crecimiento. Hemos tenido 15 días desde la germinación. Venga va, día 10/02/23 meto unas fotos mas, llevamos 17 días desde la germinación

Week5 one plant is doing pretty good. Decided to go for topping then bending, I also cut the lowest branches. The other plant seems to have had too much heat stress and have not grown much.. Especially two of them are suffering so I put them back inside and trying now to make them recover by providing them some humidity with plastic bottles, should work hopefully

Lemon Cake harvested

Late flowering

Day 53 has broken for the ladies, and I am very pleased with their progress. The buds are visibly getting stronger every day. A few brown leaf tips appeared, but at this stage I don’t care much for them. Overall, they still look very much alive. Day 57: Uppermost leaves are curling up. Could be they are saturated with light and I should reduce the lighting period a bit, could be that the water drop installment provided them with a bit too much water, although they don’t show any more fresh signs of over-watering or fertilisation. But I had to remove water that flooded the bottom of the tank … I’ll reduce their lighting period to 14 hours. Rest of the ladies looks very healthy so I don’t want to stress them too much. It would be better to raise the left light a bit but I cannot. So, although the label says they would grow taller, I can be glad they did not gain more height. Day 58: Buds are really getting massive, and they started to smell a bit more. They even gained 1 cm and tallest bud is now at 89. It’s a very sweet but deep odour, really enjoyable. Lady #2, while being a bit smaller, looks like being in winter. Sugar leaves are enormous on her. I added a detail photo from timelapse video showing some of the curled and slightly burnt leaves. They are not many, and damage does not seem to progress. Anyway, ladies are already late in the game and I am rather surprised they still look very healthy.

My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems.

Week #4 KICKASS AUTO / KANNABIA Week #4 Veg she stated to show signs of pre flower late this week additionally she starting to stretch so I'm sure that she about to go into pre flower. She doing really well outside no issues this week to report! Kannabia.com KICKASS AUTO

D65 Just a few days until the harvest, I'm checking the evolution of the trichomes and buds, we'll see what happens, I spent a lot of energy and hours I hope it will be worth it, meanwhile in my house there is the scent of ripe oranges and strawberries I hope to find the same aroma in the smoke. D68 Tomorrow early morning I will harvest Purple Lemonade, no words for describe this magnificent plant! D69 Tomorrow early morning I will harvest Banana Purple Punch and Cream cookies

Week 11 day 78, you can see buds are really fattening up, some good trichome production as well, The smell is really awesome , sort of a sweet flowerish cannabis smell. All my other plants has grown big as well so there is not many room left. i did some defoliation but you hardly notice.😁 i gave a PK boost again this week also