Giorno 63 Alcune piante a breve verranno raccolte altre proseguono la loro fioritura. A settimana prossima 🖐️ ❤️

We are going to put the smaller clones higher (on a table or whatever) and soon we will start to flower, i would have flowered earlier but these girls are sharing room with other plants

Smell is sweet and fruity, but dark...... Starting to pack in on , buds are so dense

The DoSiDos is still growing more pistils daily. The buds are swelling very well and it looks like she still has a week or 2 left before harvest. The humidity in the tent is a bit high (56%) so I moved the de-humidifier in the room to keep it below 50% and prevent budrot. The smell is just great, and still getting stronger each day as she matures. Because the DoSiDos is still growing quite a few white pistils, I have added a 40W red Led to the tent. The red light should stimulate the DoSiDos to speed up the blooming. Trouble in paradise this Saturday.I noticed a strange looking brownish leaf in the main cola. On further inspection it was full of budrot 😱. Luckily it was the top part of the main cola and I managed to keep most of the plant and none of the other buds had any mould inside. It's a real shame, but this can happen when experimental manoeuvres like dragging your plant inside again in the late stages of bloom. Well, let's see if I can keep her in one piece for at least a week more. The trichromes are still mostly transparant so she needs more time to mature.

Plant is growing well. Probably the prettiest one outta the bunch so far.

At the start of my first proper week of growth she seemed to be doing really well. I noticed her stretching quite a bit, so I moved to the light a bit closer. This seems to have done the trick. Thanks to everyone on Grow Diaries who answered my question about burying some of the stem when transplanting. I decided to move to a slightly bigger pot (from 1litre to 3.8 litres) so that I could bury some of her stretchyness. This was based on advice from you guys - so thank you! She seems to have taken to the transplant well. I made sure the EC and PH of both mediums were very close.

All content on this diary is for inspirational and educational purposes only. The ideas shared are not a substitute for professional advice. This diary/account is not officially affiliated with Alan Watts or his estate. All materials are used under the principles of fair use. I honor the legacy of Alan Watts by sharing his wisdom respectfully and with the intention of inspiring awareness and self-understanding. 2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

04/11 - Both plants are coming along accordingly on time - As stated previously I got careless with the PH and they suffered a harsh PH burn - Since then they have been flushed and nutes in coco stripped - Began introducing nute in micro-dosing stages. 04/18 - Both ladies have rebounded from PH burn - They will run for about another 2 weeks in the 1 gallon pots and then be transplanted into 5 gallon pots - I have decided to "NOT" clone these and run them as pure mothers.

Im changed my lamp from 450w cob diy led to 600w hps sylvania grolux :) will see

Eccoci qui... Siamo quasi alla fine del cultivo, odore, resina e colore ci sono. Attendiamo solo la maturazione delle cime che richiederà 1/2 settimane.... NON VEDO L'ORAAAA... Seguiranno aggiornamenti, grazie a tutti per il supporto🔥🌲❤️

Pre-flowering

Fo' Twenny here with an update on our little Gorilla x Cookies cultivar from Expert Seeds! 9/16 - Day 12: She is a beautiful shade of green and looks happy. I was one square pots short and when I bought this one, I accidentally got the 7" x 7" instead of the 6" x 6". It will bug me, because I am weird, but I planted her in it anyway. I put 1 tbsp Mycos, .5 tsp Recharge, and .25 tsp of Azos in the whole at the root zone. Sprinkled some White Dutch Clover seed after transplanting. Watered with plain water. BAD NEWS: Half of the blue diodes have gone out in the TMLAPY light. This light was actually gifted to me shortly after a friend purchased it from Amazon and realized it was too small for their space. It seemed to be serving it's purpose well for vegging photos and running bumper crops of autoflowers but it failed only four months after the warranty period ended! Weak-Sauce! GOOD NEWS: So, I upgraded the 24"x48" tent to Samsung LM301H boards. I ordered a 320w straight single piece heatsink with 3 boards from Geeklight. I went with the 3k spectrum and switchable UV/IR since all my autoflowers bloom under this light. $306 USD after all is said and done. https://app.alibaba.com/dynamiclink?touchId=1600101815945&type=product&schema=enalibaba%3A%2F%2Fdetail%3Fid%3D1600101815945%26ck%3Dshare_detail&ck=share_detail&shareScene=buyer 9/17 - Day 26: Installed new light NUTRITION: Mixing rundown Foliar Feed & Nutrient Solutiin 2 gal tap h2O run through x2 KDF filters Start ppm 630 Start ph 7.8 1 tsp/gal Gold Shield Silica Supplement 1 tsp/gal Fox Farm Gringo Rasta Cal-Mag 8.1 ph .33 tsp gal ph down pH: 6.5 PPM: 730 Serenade 3 tsp/gal (Always adjust pH prior to adding any microbial innocclants) Filled Spray bottle with 32 oz of the 2 gal mixed solution. Add 2 tsp/gal of Fox Farm Flower Kiss (.5 tsp 32oz) 6 tsp/gal Optic Foliar Transport PPM: 749 pH: 6.4 Added 1 tsp Recharge to the remainder of feed solution (to 1.75 gal) in the sprayer. Thanks for stopping by my garden! Please check out my other diaries & give me a follow! Happy growing my friends! ☺️🌱 Peace, ☮️✌️ Love, 💚🤟 & Frosty Nugs! ❄️🌲 - Fo' Twenny

Things looking good bud getting bigger smell getting stronger !

huge cannopy for only 104watts sick light

Checked the trichomes of both Guava and Frostbanger and they aren't ready yet. Still about 75-80% milky/cloudy and 20-25% clear. The only amber trichomes are on the sugar leaves and fan leaves. I'm surprised how purple/black Guava has gotten since it's not supposed to be that color and is supposed to be sativa-dominant, not short like it is, but certainly not complaining because this pheno will get an A+ for bag appeal! Gelato has some nice purpling now too. I've been flushing Guava for a while but I'll probably start flushing the others since they're probably only a week or two behind Guava. The Zamnesia strains seem to be maturing the slowest of the lot, but still seem perfectly healthy. I've been removing yellow fan leaves and tying up sagging buds as needed. Included a video this time, so check it out!

7

Plantas sanitas .

Today 5/7/25 she turn exactly 14 weeks Some leaves were brown and crunchy, didn't want it to get to buds Also a bit of lightdamage on the top, light was a bit too close, too bad I'm going to leave the bottom for a few days, see if a lot changes or she gains She is still swelling a bit, some parts are sproutin She is gaining nicely, getting her bottom to fat 😛 Her bottom buds have grown nicely in size. 13/07/25, cut bottom, most... another 70 gr, very happy! Leave smallest buds 1 day Tea comin up. Her stem is 100% citrus yummy smell!! Coocked everthing wit saffraan and a big spoon of honey. Ir tasted wondeful! Good tea man ❤️ Bottom crack was another 20gr wet In total 46gr

Just perfect harvest thanks to Platinium for their great fertilizer and to barney's farm for this strain that I recommend a just incredible taste🤤🤤🙏