09/06 - DAY 43 - Slight yellowing and spotting on a few of Mo’s older fan leaves, thinking Mag deficiency. Everyday more and more trichomes coming in. - Forcing Jane to open up by pulling her out. 09/08 - DAY 45 - Top dressed Mo w/ Worm Castings & Kashi Blend. Can see feeder roots everywhere under the cover, worms are active, and she’s sucking dry her reservoir every 1.5 days. - Lowered light to 500-560PPFD at canopy. Humidity ~58%. - Jane is starting to share her strawberry aromas. Mo smells like a sweet sugary treat 09/11 - DAY 48 - Switched over to only blooming/fruiting inoculants & top watered w/ compost tea. Hopefully get ahead of any deficiencies. - Mo is starting to drink 2 Gal in less than a day, along with the weekly top waterings. Thirsty girl 😃

Last week, first of flowering, was outstanding, I could water the girls regulary and the work paid off they stretched a lot and are looking super healthy. The girl right below the upper fan is suffering a bit with the shadows and is notoriously shorter than her sisters, I will need to rethink the air circulation for the next grow. Another concern of mine is their height, hoping they dont stretch anymore as the lights are already at maximum possible height inside tent and there is only 20cm gap between it and the plants.

Good

Day 14 - 3 out of 5 are growing a little slow/weird, all 5 seem to be growing kinda slow compared to my first run of autos but hopefully everything does good🤞🏼 Happy growing friends🌱✌️🏼 Day 17 - Still have 2 extremely weird looking ones, really hoping they bounce back🙏🏼🤞🏼 Day 18 - 3 out of 5 still going strong, the other 2 havnt changed much!

Hi! As expected I harvest both plants on the same day. I removed the biggest leaves and now their drying inside the same tent they grew. Humidity is about 62%, which is an very good humidity level, I’m not using humidifier nor ac unit, temperature are between 25-28 degrees Celsius. I amended the soil with wormcasting, some to dust and organic nutrients to prepare for the next cycle, the bed is covered waiting the plants to finish drying and the new light panel to be installed. I’m expecting a drying period of 10 days since humidity is stable at a good range. Thanks for the comments and followers on this cycle. Stay tuned for the next one! Peace!

Today the ladies finally are cooling off a break in the extreme temperatures of the summer after a long Heatwave

Plants are getting big enough to train through the fence. Im thinking a vertical scrog, but its gonna be a bitch to harvest . We will see, still dunno if they are even female.

420 Fastbuds Amnesia Haze Auto Week 8 This week has been an interesting one to say the lease. It's been in the 100's in my grow tent even with air circulation. So I decided since my outdoor has been growing good I put them with um. Over all still watering straight well water 2 liters daily with no nutes. I do spray once a week for preventive maintenance with no issues. All in all Happy Growing.

🌱 Grow Diary Update 🌸 What’s up! It’s Week 7, Day 1 of flower, and the girls are keeping me on my toes. 🌿 After getting the PPMs back in range last time, they’ve crept up again, so it’s flush time once more. 💧 I think letting the coco dry out too much between waterings might’ve been the culprit. Lesson learned—keeping a closer eye on that from now on! Besides that little hiccup, everything’s looking good. These buds are getting juicier and stinkier by the day, and there’s no serious nutrient burn, so we’re still in the clear. 🙌 Wish me luck as I ride out these last couple of weeks—hoping to finish strong! 🍀 Catch you in the next update! 💚

My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems.

Hey Growmies, Let’s kick off another competition journey — this time with Auto Glue XXL from Izzi Seeds! Every time I see “Glue,” my expectations automatically go up 😄 let’s see what this girl has to offer. It’s my first time running seeds from Izi, so curiosity is high. Let the journey begin 😉🌱

This plant is getting close to the finish line. She doesn’t have the tightest buds I’ve seen but what she lacks in tightness she makes up with loads of trichomes and a nice sweet skunky smell. I will likely take her down in the next couple days. She has been a pleasure to grow. Zero issues with mould/mildew or bugs! A true winner when it comes to resistance! Cant wait to chop!! Thanks for supplying the genetics sweet seeds!! 😎🍿🍻🌱

Day 49 update (Sunday 7/14/19): Almost time to chop! I think my humidity is finally going to be manageable for the drying- the second dehumidifier helped enough it seems. I cut out the base nutes (which for a few days were at quarter strength). I decided to stop with sugaree. No more cal/mag - I guess I should have cut that out weeks ago even in coco? So many different trains of thought. So I’ve now been flushing for a couple days using only water and canna boost and cannazyme along with mammoth p and hydroguard (ppm is at 200). I’m not PH correcting the reservoir tank and it’s up to 6.9/7.0 ph (I actually added a tiny bit of ph down before I decided to not worry about it) - I’m not sure if I like this strategy (seems to be an unsettled debate about ph correcting during final flush) I’ll switch to FloraKleen and water only (and still not ph corrected) for the last few days (in a few days or so - maybe a bit longer) THOUGHTS ANYONE?? I went to bed last night looking at pictures of bud rot and when I woke up I was convinced I had some. I actually chopped my first bud off the plants! one of my possible mold suspects (and one of the largest). Manhandled it - dug around - (I did use gloves and I did sorta try to be careful but...) I probably lost and damaged a bazillion trichomes - but.... it’s fine! No mold/bud rot at all. Beautiful actually. Some of the little fan leaves just turned prematurely yellow awhile ago and some extra areas of red hairs that concerned me but It’s great! Super stinky. Super nice. Dense. Pretty. I’m hanging that one nice big bud in the flower tent above the lights and in the corner away from a direct fan breeze (this is not at all ideal - the main harvest will be done in darkness; with no other humid plants in the tent; with proper air flow; lower temps; and they’ll have had a full flush unlike this nice cola I chopped with its “partial” flush only (see above) . It’ll be nice to experiment with taste before and after a flush since there seems to be so much debate about this. But I suppose my dry will differ too so not a very controlled test. This bud seems to be plenty mature and ripened - some amber trichomes - so I think this one bud chop may turn out to be a nice experiment caused by my neurosis about non existent bud rot) Woot.