2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Bueno resumiendo cookies gelato es una variedad híbrida muy fácil de cultivar pero ojo, cuidado con los últimos riegos si no queréis perder cosecha por moho. Lo demás de 10 pegada heavy sabor increíble, cogollos duros como rocas y bañados de una gran capa de polen. El ambiente del secado se mantuvo en 23 grados de media y la humedad estuvo por debajo de los 45% en todo momento. Poco más la verdad estuve encantado de poder cultivar una genética tan potente . Un saludazo que paséis un final de año increíble y por supuesto buenos humazooos💨💨💨.

This week everything is going perfect , purple punch is growing a very big top from other 6 seeds that i plant 10 days later 1 gorilla punch and wedding cheesecake are already flowering. I just found out that during preflower and first weeks of flower I must cut perfect ph micro to 50 % and perfect ph grow to 75 % to prevent N excess . They are growing big and healthy I still don't know what yield I should expect from these plants . Any idea

**** WEEK 10 **** both hulks shaping up to be some real beasts. just can’t beat good ol sunlight

Moving right along, each girl is on her own pace. Tent was so crowded I had to remove the heater therefore I bought a bigger tent and will use use this one for drying. Thanks for looking..

14 day dry | Curing in Grove bags, filled 7 QP bags & got 56g of keif out the trim bin from only 2 plants! More pictures coming! STAY FROSTY GROWMIES

Had great results, especially when i upgraded to RO water.. if theres one thing i could of changed it would of been using the reverse Osmosis water sooner. The rest of the grow was pretty easy, didn't even plant train as this was suppose to be a hassle free run. It was just that. Some good genetics from seedstockers too. Good stuff!

Venga familia que ya viene la cosecha de estas Apple Fritter de RoyalQueenSeeds, que ganas que tenia ya de darles machetazo. No veas que pinta que tienen estas plantas. Las flores aparte de prietas se ven bien resinosas. a sido una genética con la que disfruté mucho cultivarla, es algo complicada cultivarla pero merece la pena si eres cultivador con experiencia no te será problema cosechar. Agrobeta: https://www.agrobeta.com/agrobetatiendaonline/36-abonos-canamo Mars hydro: Code discount: EL420 https://www.mars-hydro.com/ Hasta aquí es todo , espero que lo disfrutéis, buenos humos 💨💨.

the plant has grown very well and its growth has accelerated

Now this baby was a delight to grow and grew real big. I decided to leave this lady to do her own thing and she ended up finishing just short of 6ft! The main cola was huge and juicy and all round the end result was really nice and super strong just the way it should be.

Overall this plant was great to grow, she just grew and was happy doing it the entire time, pretty good yield off a plant that was only about 12inches tall. Seemed to be a lot more resistant to deficiencies then the other two strains this grow. Definitely a great mid-day Smoke!

Week 6........... Getting frosty Edit: on day 41,I already have amber but it's still packing on weight. I don't know what to do at this point.

29.8. Plants look just amazing! I dont add any nutritions... Just bionova powder nutritions in start which i get from zamnesia 💪 maybe i found right nutritions for my plants 31.8. I will leave those beautys for few days more 😍 i cant wait to taste this buds! 2.9. Another week more and they are done 🤟 so far im impressed 🚀

Disculpen las fotos, es que las saque para consultar. Bueno primero que nada fue una semana de mucha tormanta, lluvias y humedad. lo peor que nos puede pasar en floracion. Segundo intente hacer supercroping ya que las ramas seguian superando el tejido y les llegaba luz en la noche. Y me salio desastrozo, mi primera vez. Pero los otros punteros que superaban la altura, los hizo mi compañero que sabia hacerlo (No tengo foto, luego actualizo). Y Tercero que debido a la humedad se ven en las fotos como aparecio hongos, por lo que pude observar era solo ese poco, lo cual ya fue removido vamos a ver como continuamos.

This Update came a little bit late cuz time issues but here is it :) Last week i had solution issues and the plants go very fast in bad leaf color. The Solution got too warm in the tanks and the water got a bad smell too. I changed the water and made again the 3rd week nutrition and turned up the fan to get a few degrees colder it worked very well. Roots are so far doing nice and have no burns or rotten parts on nice and white. Then i installed the second light in. The week before i give them 400 W MH and 100 W LED. Now we have 2x 600W and 100 W LED each one turned down to 250 W and the going good. But i had only 1 bulb m MH and so im now have a mixed light end of this week i give them 1 tsp Mineral Magic over the stem to get them stronger Mostly of them are Topped and have not bad mooding on the growing stage of the plants. I hope on get a few Tips on what we can do better on the System Best Regards :) and Good Groow @all

Sorry for the late post had a very busy period. The buds are great, good feedback from patients. First time I've been able to taste a fully organic bud and I can say I won't be going back!

I removed the LST ties about 6weeks in and added Bloom when she was showing pre flowers although it states in the nutrient list I was adding both bloom basic and biobizz bloom I actually cut out the biobizz at week 9 and replaced it with Atami bloombastic.

Semana del flush 🚿🤩 empecé a hacer el flush desde la semana pasada, desde el miércoles, y la coseche el día viernes de esta semana, ahorita ya las tengo curándose, se secaron muy rápido en cuestión de 3 días 😥 Pero se ven muy bien, en una semana subiré los resultados finales 😄

Calyxes are stacking and getting fat outside. Definitely in prebloom Indoor plants are still adjusting to grow lights so I'm gonna give them a few more days Day 1 of flower for indoor is august 9th