Days 92 - 98 (from sprout) 8/23/24 - 8/29/24 Loompa's Headband x TK fading hard this week - checking trichome color through a 15x loupe, needs more time The Good Shit drinking a gallon of water a day but slowly backed off near end of week - my guess would be a 14-week flowering period for this genotype before harvest quality I'm pretty much watering daily a 1/4 gallon to Blue Nose Pit or less to keep mulch layer from drying out Sweet 16 S1 takes the win for most trichome coverage out of the garden, has the most up-front aroma out of the geno hunt PAR is all over the place on this one and totally messed up, after this week I'll raise the light and focus on the appropriate PAR for canopy of The Good Shit Pest Management slipping too, fungus gnats are repopulating and it seems the ecosystem has encountered a serious imbalance of beneficials and predators that normally kept soil/mulch in-check Plan on short-term remedying this with a top-dress of remaining compost + em bokashi and a moderate drench of EM5 following with repeated treatments of enzymes (tweetmint) Long term solution may need a predator/beneficials kit and/or higher quality vermicompost after knocking them back with enzymes

Die Buds sind bis jetzt noch nicht so sehr groß geworden. Was aber nicht ist, kann noch werden. Sehr intensiver süßer, leicht gasischer Geruch.

Week 7 of 9 for Do-si-dos, this strain is running 1 week longer than the others. Overall she's the least nutrient hungry of the group and shows the least amount of deficiency. Still, I fed her and extra liter last week. August 1 - added close up pictures.

Hello my friends 👨‍🌾👩‍🌾, I've transplanted the girls in 11l pot today, did a defoliation. The girls started to be really unhappy 🙁 in 2l pot. I continue my training for the scrog. In the 11l pot, in the down, Plagron Batmix, on the top Plagrin light mix. It will be the definitive pots. 💦 I give water each 2 or 3 days 500 ml/plant Water + Rhizo + Terra vega Water + Rhizo + Cannazym After Transplanting 0.95 l/plant Water + Rhizo PH@6 I've down the Lamp @90% Bisous 💋😘, and see you next week. Thanks community for follow, likes, comments, always a pleasure 👩‍🌾👨‍🌾❤️🌲 Mars Hydro - TS 1000 https://www.mars-hydro.com/ts-1000-led-grow-light Mars Hydro - FC3000 https://www.mars-hydro.com/fc-3000-samsung-lm301b-led-grow-light Mars Hydro - SP3000 https://www.mars-hydro.com/sp-3000-samsung-lm301b-greenhouse-led-grow-light The High Chameleon - Bisous Au THC 💋💋🌲🌲😘😘 https://www.thehighchameleon.com/shop/bisous-au-thc-83

23.10. Day 53 of flower. The buds become flesh. Still wonderful sticky. She really likes it moist. Lifted the lamp and switched the white Light off. Just for a while. Because, i think she began to be grilled. Just a little relax for her. Switched to 16_8h light nightschedule. And agaun with white light, FULL POWER. With greetings from the unexpected colorful Trans Syberia..😛 💪

May have added more nutrients forgeting the pot already came with its own nutrients for the seed. So just add 6.5 water. I’ve decided to add Fish shit to boost the growth along the way. Don’t know if it’s needed or not. The sprout is growing its first set of fan leaves which appear to have a couple spots. I left the soil uncovered for about a week so nutrients may be for concentrated at the top of soil causing slight nutrient burn spots… I’ll just keep using decholinated 6.5 water

Good week. On day 50 I Defoliated. Removed lowers, Taped up the room for light blockage Day 56 from Complete reservoir change Room temp/humidity- 67/70 Plant temp/humidity-70/65 CO2 level-922 CO-47 Water ph- 5.9 Water ppm- 1200 Water temp- 73

———GENERAL COMMENT ——— Flushing for around 10 days, they will fall asap. ———BLUEBERRY COMMENT——— Taking off the cage i broke a cola stem for that she ended looking unbalanced... stems are stiff as iron.

Been flushing in anticipation of cutting down. Day 100 (yes I'm late) chopped the two main colas. They are very dense and heavy!!!!! Just a few more days for the rest of the plant.

Día 14 comenzando la 3ra semana de vida, todas tienen un buen desarrollo, solo hay 1 que presenta una coloración distinta y una morfología diferente a las demás, probablemente sea solo un fenotipo distinto, además plante una nueva semilla en la maceta que estaba vacía

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Привет друзья. Моей растихе сегодня 48 дней. Начал применять LST технику на 19 дне и продалжаю применять её через день, а 18 августа добавил ДЕФОЛИЗАЦИЮ С 20.08 ДЕФОЛИЗАЦИЮ делаю каждые 3,4 дня С 20.08 LST технику делаю каждые 4.6 дней На сегодняшний день влажность 50% Три дня назад заметил высокий Ph 7.9 С сегодняшнего дня Ph 5.8 Заказал погладитель влажности, отпишусь в следующем репорте. Не смотря на высокую влажность и высокий Ph, растение растёт хорошо, генетика радует. Всем мира и добра! https://t.me/smail_seeds #Smail_Seeds

My permanent marker plants are looking really interesting! The short and stocky one with the purple leaves is super vibrant. I love that pop of color against the green of the other plants. It's a little different from the taller plant, which has this really cool stacked-up look to it. I'm curious to see if those stacked-up leaves will change color too. The past few weeks have been so beautiful, watching the plants grow and change. It's amazing how something so small and fragile can turn into something so strong and vibrant. Now that they're getting ready to bloom, it feels a little bittersweet. I'm so excited to see the finished product, but I'll definitely miss having them around while they're growing.

I busted myself up so havent been able to get into the grow much for a couple weeks. They are definitely thirsty and not getting enough water so will water more deeply next time around a gallon per tote a day.

Plants in containers seem to be 2 weeks ahead of plant in ground, marigolds dping great

Hi guys, this week has been great for plant growth..they are 85cm tall and I think they will grow a little longer. You can see the future buds that will be covered with flowers. From this growth I am learning that the FIM technique is better because the buds will all be at the same height and look much more (as you can see in plant number 4). Pistils begin to appear and in the next week I hope to see more pistils. Stay Tuned 🦍